Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,533,538 A→T 11.5% intergenic (+58/‑7) fimA → / → fimI major type 1 subunit fimbrin (pilin)/fimbrial protein involved in type 1 pilus biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,533,5380AT11.5% 560.9 / 54.0 191intergenic (+58/‑7)fimA/fimImajor type 1 subunit fimbrin (pilin)/fimbrial protein involved in type 1 pilus biosynthesis
Reads supporting (aligned to +/- strand):  ref base A (74/95);  new base T (9/13);  total (83/108)
Fisher's exact test for biased strand distribution p-value = 8.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 204 total aligned reads displayed.

ATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCC  >  CP009273/4533391‑4533685
                                                                                                                                                   |                                                                                                                                                   
aTTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATaaaa                                                                                                                                                     <  2:458430/148‑1 (MQ=255)
 ttCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATaaaaa                                                                                                                                                    >  1:3264728/1‑148 (MQ=255)
 ttCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATaaaaa                                                                                                                                                    >  2:665488/1‑148 (MQ=255)
   ccGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTNCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATa                                                                                                                                                  <  1:1801352/148‑1 (MQ=255)
     gTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGTACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAAc                                                                                                                                                >  1:1853535/1‑148 (MQ=255)
     gTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAAc                                                                                                                                                >  1:513319/1‑148 (MQ=255)
       tCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGa                                                                                                                                              <  1:344156/148‑1 (MQ=255)
       tCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGa                                                                                                                                              >  1:1848991/1‑148 (MQ=255)
          aGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGa                                                                                                                                           <  1:2203405/148‑1 (MQ=255)
           ggCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGaa                                                                                                                                          >  2:447086/1‑148 (MQ=255)
           ggCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGaa                                                                                                                                          >  1:1735159/1‑148 (MQ=255)
            gcgcGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGaaa                                                                                                                                         >  2:2870908/1‑148 (MQ=255)
              gcgTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAg                                                                                                                                       <  1:998726/148‑1 (MQ=255)
              gcgTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAg                                                                                                                                       <  2:2428762/148‑1 (MQ=255)
               cgTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAgg                                                                                                                                      <  1:1328356/148‑1 (MQ=255)
                gTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGa                                                                                                                                     <  2:1733982/148‑1 (MQ=255)
                gTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGACGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGa                                                                                                                                     <  2:1698499/148‑1 (MQ=255)
                  tATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAag                                                                                                                                   <  2:1323233/148‑1 (MQ=255)
                  tATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAag                                                                                                                                   >  2:2317575/1‑148 (MQ=255)
                    ttttGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAagag                                                                                                                                 <  1:411877/148‑1 (MQ=255)
                    ttttGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCCACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAagag                                                                                                                                 <  1:412305/148‑1 (MQ=255)
                      ttGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGat                                                                                                                               <  1:250484/148‑1 (MQ=255)
                       tGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGatt                                                                                                                              >  1:1511536/1‑148 (MQ=255)
                        gCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGatta                                                                                                                             <  1:2006137/148‑1 (MQ=255)
                         cAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGattat                                                                                                                            <  2:1609841/148‑1 (MQ=255)
                           aCCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTAttt                                                                                                                          >  2:2126707/1‑148 (MQ=255)
                           aCCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTAttt                                                                                                                          >  2:642594/1‑148 (MQ=255)
                           aCCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTAttt                                                                                                                          >  1:564632/1‑148 (MQ=255)
                            ccGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTc                                                                                                                         >  2:298153/1‑148 (MQ=255)
                             cGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCt                                                                                                                        <  2:370321/148‑1 (MQ=255)
                               gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTAt                                                                                                                      <  1:1431898/148‑1 (MQ=255)
                               gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTAt                                                                                                                      <  2:587068/148‑1 (MQ=255)
                                     cAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgt                                                                                                                >  2:3484314/1‑148 (MQ=255)
                                     cAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGcgt                                                                                                                <  2:1848991/148‑1 (MQ=255)
                                         cccGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGtt                                                                                                            <  1:919885/148‑1 (MQ=255)
                                              gTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTtgctgc                                                                                                       >  2:424542/1‑148 (MQ=255)
                                                    ctAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGt                                                                                                 >  1:3511598/1‑148 (MQ=255)
                                                     tAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGtt                                                                                                >  1:156353/1‑148 (MQ=255)
                                                      aaTGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGttt                                                                                               <  2:1108276/148‑1 (MQ=255)
                                                        tGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGc                                                                                             >  1:2224281/1‑148 (MQ=255)
                                                          cGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGctc                                                                                           <  1:970364/148‑1 (MQ=255)
                                                          cGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGctc                                                                                           >  2:196745/1‑148 (MQ=255)
                                                           ggATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGctct                                                                                          <  2:2951679/148‑1 (MQ=255)
                                                            gATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTg                                                                                         <  1:1723501/148‑1 (MQ=255)
                                                             aTGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTgg                                                                                        >  1:2968490/1‑148 (MQ=255)
                                                             aTGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTgg                                                                                        <  2:792943/148‑1 (MQ=255)
                                                                   ccTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaa                                                                                  <  1:888707/148‑1 (MQ=255)
                                                                    cTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaa                                                                                 >  2:2005593/1‑148 (MQ=255)
                                                                     ttCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaat                                                                                <  1:1727010/148‑1 (MQ=255)
                                                                      tCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaata                                                                               <  2:721895/148‑1 (MQ=255)
                                                                       cAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataa                                                                              >  1:347297/1‑148 (MQ=255)
                                                                        aaGGTTCAGTATCAATACCCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataaa                                                                             <  1:1524408/148‑1 (MQ=255)
                                                                        aaGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataaa                                                                             <  2:687779/148‑1 (MQ=255)
                                                                         aGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGTAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataaat                                                                            >  1:239325/1‑148 (MQ=255)
                                                                         aGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGaaataaat                                                                            <  1:1259031/148‑1 (MQ=255)
                                                                          ggTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATg                                                                           <  1:2712872/148‑1 (MQ=255)
                                                                          ggTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAACGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATg                                                                           >  2:957622/1‑148 (MQ=255)
                                                                             tCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGaa                                                                        >  1:949071/1‑148 (MQ=255)
                                                                             tCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGaa                                                                        >  1:1964838/1‑148 (MQ=255)
                                                                                gTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATAc                                                                     <  2:2739969/148‑1 (MQ=255)
                                                                                  aTCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGTATGCCCACCCTTGTGCTATAAAAATAACGATGAAAAGGAAGAGAGTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCa                                                                   <  1:2870347/148‑1 (MQ=255)
                                                                                  aTCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCa                                                                   <  1:1778347/148‑1 (MQ=255)
                                                                                  aTCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCa                                                                   >  1:1227299/1‑148 (MQ=255)
                                                                                   tCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCAc                                                                  >  1:385194/1‑148 (MQ=255)
                                                                                    cAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACg                                                                 <  1:2612852/148‑1 (MQ=255)
                                                                                       tAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTg                                                              >  2:3276099/1‑148 (MQ=255)
                                                                                                 aGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGaa                                                    >  2:2782303/1‑148 (MQ=255)
                                                                                                  ggTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGACAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGaaa                                                   >  2:2256875/1‑148 (MQ=255)
                                                                                                    ttCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAata                                                 <  1:2171668/148‑1 (MQ=255)
                                                                                                      cAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATg                                               >  1:2149090/1‑148 (MQ=255)
                                                                                                        gggACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCa                                             <  2:1298187/148‑1 (MQ=255)
                                                                                                        gggACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCa                                             <  1:1489837/148‑1 (MQ=255)
                                                                                                         ggACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCaa                                            >  2:456136/1‑148 (MQ=255)
                                                                                                         ggACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCaa                                            <  1:1280376/148‑1 (MQ=255)
                                                                                                          gACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAAt                                           <  2:2520775/148‑1 (MQ=255)
                                                                                                            cGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAAttt                                         >  1:1386640/1‑148 (MQ=255)
                                                                                                               cATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAg                                      >  1:3185265/1‑148 (MQ=255)
                                                                                                               cATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAg                                      >  2:3329547/1‑148 (MQ=255)
                                                                                                                 ttACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAggg                                    <  2:829927/148‑1 (MQ=255)
                                                                                                                  tACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGc                                   >  1:1878033/1‑148 (MQ=255)
                                                                                                                    cGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGt                                 <  2:2221536/148‑1 (MQ=255)
                                                                                                                     gggCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTc                                <  1:1056793/148‑1 (MQ=255)
                                                                                                                          gggATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCattat                           <  1:285645/148‑1 (MQ=255)
                                                                                                                            gATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTg                         >  1:442157/1‑148 (MQ=255)
                                                                                                                                  cACCCTTGTGCGATAACAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGaaa                   <  2:2935357/148‑1 (MQ=255)
                                                                                                                                  cACCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGATCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGaaa                   <  1:3128082/148‑1 (MQ=255)
                                                                                                                                  cACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGaaa                   <  1:1157909/148‑1 (MQ=255)
                                                                                                                                   aCCCTTGTGCGATAAATATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAAc                  >  1:775040/1‑148 (MQ=255)
                                                                                                                                   aCCCTTGTGCGATAAAAATAACGATTAAAAGGAAGAGATTATTTCTATTAGAGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAAc                  <  1:2317543/148‑1 (MQ=255)
                                                                                                                                   aCCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAAc                  >  2:531514/1‑148 (MQ=255)
                                                                                                                                   aCCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAAc                  <  1:2317613/148‑1 (MQ=255)
                                                                                                                                   aCCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAAc                  >  1:353598/1‑148 (MQ=255)
                                                                                                                                      cTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTg               >  1:1576772/1‑148 (MQ=255)
                                                                                                                                       ttGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCGCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATATCAGGGCGTCATTATTGCGGAAACTTGc              >  2:2330246/1‑148 (MQ=255)
                                                                                                                                        tgtgCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGcc             <  2:3264728/148‑1 (MQ=255)
                                                                                                                                            cGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGAt         <  1:665488/148‑1 (MQ=255)
                                                                                                                                                aaaaaTAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGaa     <  1:2767677/148‑1 (MQ=255)
                                                                                                                                                 aaaaTAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAg    >  1:152558/1‑148 (MQ=255)
                                                                                                                                                  aaaTAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGc   >  1:1697696/1‑148 (MQ=255)
                                                                                                                                                  aaaTAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGc   >  2:2725555/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATTCCGTTCCAGGCGCGTTATTTTGCAACCGGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGACGTCATTACGGGCAGGGATGCCCACCCTTGTGCGATAAAAATAACGATGAAAAGGAAGAGATTATTTCTATTAGCGTCGTTGCTGCCAATGTTTGCTCTGGCCGGAAATAAATGGAATACCACGTTGCCCGGCGGAAATATGCAATTTCAGGGCGTCATTATTGCGGAAACTTGCCGGATTGAAGCC  >  CP009273/4533391‑4533685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑