Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,545,107 G→T 12.4% D239Y (GAT→TAT)  uxuR → fructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,545,1070GT12.4% 804.7 / 58.9 249D239Y (GAT→TAT) uxuRfructuronate‑inducible hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (113/105);  new base T (9/22);  total (122/127)
Fisher's exact test for biased strand distribution p-value = 2.10e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 249 total aligned reads displayed.

ACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCAGGG  >  CP009273/4544961‑4545253
                                                                                                                                                  |                                                                                                                                                  
aCAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGa                                                                                                                                                   >  2:248943/1‑148 (MQ=255)
aCAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGa                                                                                                                                                   >  2:2407791/1‑148 (MQ=255)
  aGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGata                                                                                                                                                 >  2:3160492/1‑148 (MQ=255)
   gCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCTAACGTTGACGatat                                                                                                                                                >  1:2674289/1‑148 (MQ=255)
     cTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGAtattt                                                                                                                                              <  2:731091/148‑1 (MQ=255)
      tGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGAtattta                                                                                                                                             >  1:1670477/1‑148 (MQ=255)
       gTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGAtatttat                                                                                                                                            <  1:2275699/148‑1 (MQ=255)
        tATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTAtatttatt                                                                                                                                           >  1:3485754/1‑148 (MQ=255)
         aTCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATatttattt                                                                                                                                          >  2:1055524/1‑148 (MQ=255)
         aTCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATatttattt                                                                                                                                          <  1:1115294/148‑1 (MQ=255)
            gCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGa                                                                                                                                       >  1:2962613/1‑148 (MQ=255)
             cAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGAt                                                                                                                                      >  2:866703/1‑148 (MQ=255)
               aaGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGAttg                                                                                                                                    >  1:2162772/1‑146 (MQ=255)
               aaGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGAttg                                                                                                                                    >  1:2163933/1‑146 (MQ=255)
                agagTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGc                                                                                                                                   >  2:979647/1‑148 (MQ=255)
                        tGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGtt                                                                                                                           <  2:2756252/148‑1 (MQ=255)
                        tGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGtt                                                                                                                           <  2:2038348/148‑1 (MQ=255)
                          ggCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTg                                                                                                                         >  1:534079/1‑148 (MQ=255)
                          ggCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTg                                                                                                                         >  2:713631/1‑148 (MQ=255)
                            cGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGAt                                                                                                                       >  1:3407861/1‑148 (MQ=255)
                              aTCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTc                                                                                                                     >  2:2844157/1‑148 (MQ=255)
                                cacaAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCAt                                                                                                                   >  1:3365135/1‑148 (MQ=255)
                                cacaAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCAt                                                                                                                   >  1:2229096/1‑148 (MQ=255)
                                  caAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCCATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATgg                                                                                                                 >  1:538164/1‑148 (MQ=255)
                                   aaaCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGc                                                                                                                >  2:1035141/1‑148 (MQ=255)
                                     aCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCg                                                                                                              >  2:2616169/1‑148 (MQ=255)
                                     aCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCg                                                                                                              <  1:2055505/148‑1 (MQ=255)
                                     aCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCg                                                                                                              >  1:3266862/1‑148 (MQ=255)
                                     aCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCg                                                                                                              >  1:1314796/1‑148 (MQ=255)
                                     aCAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCg                                                                                                              >  1:2125125/1‑148 (MQ=255)
                                       aGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCt                                                                                                            >  1:2653300/1‑148 (MQ=255)
                                          tCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGa                                                                                                         <  1:1552673/148‑1 (MQ=255)
                                          tCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGa                                                                                                         >  2:2937057/1‑148 (MQ=255)
                                             tCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATaa                                                                                                      <  2:2363632/148‑1 (MQ=255)
                                               gccgcGTTAATCAAAAAAGATGACCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAg                                                                                                    <  2:2504300/148‑1 (MQ=255)
                                                ccgcGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGt                                                                                                   <  1:1083382/148‑1 (MQ=255)
                                                 cgcgTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTc                                                                                                  <  1:180410/148‑1 (MQ=255)
                                                   cggtAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGa                                                                                                >  2:1210479/4‑148 (MQ=255)
                                                   cgTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGa                                                                                                <  1:2371819/148‑1 (MQ=255)
                                                   cgTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGa                                                                                                >  1:2428555/1‑148 (MQ=255)
                                                     ttAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACg                                                                                              <  2:114518/148‑1 (MQ=255)
                                                      tAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATCTTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGc                                                                                             >  2:2111711/1‑148 (MQ=255)
                                                       aaTCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGcc                                                                                            <  2:2414598/148‑1 (MQ=255)
                                                             aaaaGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATCAAGTCGACGCCTGACtt                                                                                      >  2:1837437/1‑148 (MQ=255)
                                                              aaaGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACtta                                                                                     <  2:3445859/148‑1 (MQ=255)
                                                                aGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACttatt                                                                                   <  1:2115735/148‑1 (MQ=255)
                                                                  aTGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTtattat                                                                                 >  1:3251668/1‑148 (MQ=255)
                                                                   tGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACTTTAAGCAACGTCTTCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGTCCGCTGGATAAAGTCGACGCCTGACTTATtata                                                                                <  2:1209556/148‑1 (MQ=255)
                                                                   tGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATtata                                                                                >  2:656212/1‑148 (MQ=255)
                                                                     cccGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATCAAGTCGACGCCTGACTTATTataat                                                                              >  2:3000307/1‑148 (MQ=255)
                                                                         aGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGACGATGGATAAAGTCGACGCCTGACTTATTATAATAAgc                                                                          >  1:3534024/1‑148 (MQ=255)
                                                                          gcggcgAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATTGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAtcg                                                                         >  1:611917/1‑148 (MQ=255)
                                                                             gcgAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCaa                                                                      >  2:576109/1‑148 (MQ=255)
                                                                                   cTGGCAATGTGGCAGCATCTGGAAAACGTTCAGCAACGTCTGCTGGAAGTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTaa                                                                <  2:2457765/148‑1 (MQ=255)
                                                                                   cTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTaa                                                                <  2:357384/148‑1 (MQ=255)
                                                                                    tGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTaaa                                                               <  1:3450846/148‑1 (MQ=255)
                                                                                     ggCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAAc                                                              >  2:193355/1‑148 (MQ=255)
                                                                                       cAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGt                                                            >  2:1050313/1‑148 (MQ=255)
                                                                                            tgGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCtt                                                       <  1:710408/148‑1 (MQ=255)
                                                                                             ggCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTg                                                      <  1:372121/148‑1 (MQ=255)
                                                                                             ggCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTg                                                      <  2:954987/148‑1 (MQ=255)
                                                                                                agcaTCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTgcgc                                                   <  1:3228676/148‑1 (MQ=255)
                                                                                                   aTCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCttt                                                <  1:3160492/148‑1 (MQ=255)
                                                                                                    tCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTc                                               <  2:3485754/148‑1 (MQ=255)
                                                                                                    tCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTc                                               <  1:3413832/148‑1 (MQ=255)
                                                                                                     cTGGAAAACGTTAAGCAACGTCTTCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCt                                              >  2:2085805/1‑148 (MQ=255)
                                                                                                     cTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCt                                              >  2:1856790/1‑148 (MQ=255)
                                                                                                     cTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCt                                              >  2:816337/1‑148 (MQ=255)
                                                                                                        gAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTaa                                           <  1:277116/148‑1 (MQ=255)
                                                                                                         aaaaCGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTaaa                                          <  2:3523765/148‑1 (MQ=255)
                                                                                                          aaaCGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAAt                                         >  2:1675722/1‑148 (MQ=255)
                                                                                                             cGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTaa                                      >  1:1275844/1‑148 (MQ=255)
                                                                                                                tAAGCAACGTCTGCTGGAATTCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTaagaa                                   <  2:2847416/148‑1 (MQ=255)
                                                                                                                 aaGCAACGTCTGCTGGAATTCTCTAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTaagaag                                  <  1:2531517/148‑1 (MQ=255)
                                                                                                                 aaGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTaagaag                                  <  2:2743665/148‑1 (MQ=255)
                                                                                                                 aaGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTaagaag                                  <  2:1977210/148‑1 (MQ=255)
                                                                                                                 aaGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTaagaag                                  <  2:848005/148‑1 (MQ=255)
                                                                                                                  aGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGt                                 <  1:995208/148‑1 (MQ=255)
                                                                                                                      aCGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCa                             <  2:3320058/148‑1 (MQ=255)
                                                                                                                      aCGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCa                             <  2:110711/148‑1 (MQ=255)
                                                                                                                       cGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCaa                            <  2:141378/148‑1 (MQ=255)
                                                                                                                         tCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATg                          >  1:997568/1‑148 (MQ=255)
                                                                                                                          ctgctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGa                         <  1:2407791/148‑1 (MQ=255)
                                                                                                                          ctgctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGa                         <  1:946195/148‑1 (MQ=255)
                                                                                                                          ctgctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGa                         <  1:2027340/148‑1 (MQ=255)
                                                                                                                            gctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGt                       >  1:2327551/1‑148 (MQ=255)
                                                                                                                            gctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGt                       <  1:3121766/148‑1 (MQ=255)
                                                                                                                             ctgGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTa                      <  1:2486945/148‑1 (MQ=255)
                                                                                                                               ggAATTATCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTAtt                    <  1:1016531/148‑1 (MQ=255)
                                                                                                                                   ttCTCGAACGTTGACTATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACtt                <  1:1751226/148‑1 (MQ=255)
                                                                                                                                       ttcctattGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTa            >  2:1882627/7‑148 (MQ=255)
                                                                                                                                          aCGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAAt         <  1:85957/148‑1 (MQ=255)
                                                                                                                                            gTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTg       <  2:534079/148‑1 (MQ=255)
                                                                                                                                            gTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTg       <  1:2937057/148‑1 (MQ=255)
                                                                                                                                             ttGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGc      >  2:3411822/1‑148 (MQ=255)
                                                                                                                                              tGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCa     <  2:408796/148‑1 (MQ=255)
                                                                                                                                              tGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCa     <  1:2617630/148‑1 (MQ=255)
                                                                                                                                              ggACGTTATTTATTTTGAGGGCTATCTGTTTGATTCATGGCCGCTGGAGAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTGTTTAATTAAGAAGTCGCAATGAGTATTAGTTTGTAAATTgga     >  2:1384976/2‑146 (MQ=255)
                                                                                                                                               gACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCAg    <  2:271427/148‑1 (MQ=255)
                                                                                                                                                 cGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCAggg  >  2:1736020/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACAGCCTGTATCGCAAAGAGTGGTTGGGCGATCACAAACAGATCCTCGCCGCGTTAATCAAAAAAGATGCCCGAGCGGCGAAGCTGGCAATGTGGCAGCATCTGGAAAACGTTAAGCAACGTCTGCTGGAATTCTCGAACGTTGACGATATTTATTTTGATGGCTATCTGTTTGATTCATGGCCGCTGGATAAAGTCGACGCCTGACTTATTATAATAAGCGCAAGGGTAAACGTTCCTTGCGCTTTCTTAAATTAAGAAGTCGCAATGAGTATTACTTTGTAAATTGCAGGG  >  CP009273/4544961‑4545253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑