Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,548,084 A→T 30.8% L8* (TTG→TAG)  hypT ← hypochlorite‑responsive transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,548,0840AT30.8% 294.4 / 185.6 201L8* (TTG→TAG) hypThypochlorite‑responsive transcription factor
Reads supporting (aligned to +/- strand):  ref base A (82/57);  new base T (33/29);  total (115/86)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

Only 100 of 209 total aligned reads displayed.

ATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGCCG  >  CP009273/4547938‑4548231
                                                                                                                                                  |                                                                                                                                                   
aTTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCaa                                                                                                                                                    >  2:655556/1‑148 (MQ=255)
 ttCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCtaa                                                                                                                                                   <  2:1144322/148‑1 (MQ=255)
  tCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCtaaa                                                                                                                                                  >  2:1390202/1‑148 (MQ=255)
     acacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCg                                                                                                                                               >  2:2945232/1‑148 (MQ=255)
      cacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGc                                                                                                                                              <  1:1822944/148‑1 (MQ=255)
      cacCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGc                                                                                                                                              <  1:737818/148‑1 (MQ=255)
       acCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGca                                                                                                                                             <  2:629380/148‑1 (MQ=255)
        ccAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGTCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGcac                                                                                                                                            <  2:462193/148‑1 (MQ=255)
         cAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcacc                                                                                                                                           >  2:1200001/1‑148 (MQ=255)
         cAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGcacc                                                                                                                                           >  2:3352054/1‑148 (MQ=255)
          aaTCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGcacca                                                                                                                                          >  2:2004242/1‑148 (MQ=255)
            tCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACcaca                                                                                                                                        >  1:814578/1‑148 (MQ=255)
            tCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACcaca                                                                                                                                        >  1:2596784/1‑148 (MQ=255)
              gCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGt                                                                                                                                      <  1:3068446/148‑1 (MQ=255)
                   ttCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATcc                                                                                                                                 <  2:1046134/148‑1 (MQ=255)
                    tCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCa                                                                                                                                <  1:1201827/148‑1 (MQ=255)
                    tCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCa                                                                                                                                <  1:2026507/148‑1 (MQ=255)
                      cAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCAtt                                                                                                                              >  2:527089/1‑148 (MQ=255)
                        gcgcACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCAGTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTAc                                                                                                                            >  2:2993299/1‑148 (MQ=255)
                            aCGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGttt                                                                                                                        >  1:3313785/1‑148 (MQ=255)
                             cGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGtttt                                                                                                                       <  1:3400803/148‑1 (MQ=255)
                                   cggcggCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTg                                                                                                                 <  2:1649577/148‑1 (MQ=255)
                                   cggcggCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTg                                                                                                                 <  2:2789447/148‑1 (MQ=255)
                                    ggcggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGt                                                                                                                <  1:3014068/148‑1 (MQ=255)
                                     gcggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTc                                                                                                               <  2:327432/148‑1 (MQ=255)
                                      cggcTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCa                                                                                                              <  1:1488902/148‑1 (MQ=255)
                                            aaTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgccgcc                                                                                                        <  1:2000076/148‑1 (MQ=255)
                                            aaTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAgccgcc                                                                                                        >  1:3519965/1‑148 (MQ=255)
                                             aTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGccc                                                                                                       <  2:2723762/148‑1 (MQ=255)
                                             aTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGccc                                                                                                       <  2:865999/148‑1 (MQ=255)
                                             aTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGccc                                                                                                       <  2:1833019/148‑1 (MQ=255)
                                             aTGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGccc                                                                                                       <  2:2374035/148‑1 (MQ=255)
                                                     tGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGc                                                                                               <  2:793877/148‑1 (MQ=255)
                                                     tGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGc                                                                                               <  2:793900/148‑1 (MQ=255)
                                                        gagaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCAtt                                                                                            <  2:835818/148‑1 (MQ=255)
                                                          gaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCt                                                                                          >  2:429368/1‑148 (MQ=255)
                                                          gaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCt                                                                                          <  2:3458403/148‑1 (MQ=255)
                                                          gaCGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCt                                                                                          >  2:712653/1‑148 (MQ=255)
                                                             gTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACg                                                                                       >  1:473262/1‑148 (MQ=255)
                                                             gTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACg                                                                                       >  1:474362/1‑148 (MQ=255)
                                                              ttGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGt                                                                                      >  2:1479783/1‑148 (MQ=255)
                                                                  gactgactGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCAt                                                                                  <  1:219118/148‑1 (MQ=255)
                                                                  gactgactGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCAt                                                                                  <  2:1866324/148‑1 (MQ=255)
                                                                           ccgccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGggcggc                                                                         >  2:1497007/1‑148 (MQ=255)
                                                                             gccTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTg                                                                       <  1:1940854/148‑1 (MQ=255)
                                                                                tGGGAAAAATTGCGGCATTTTTCCATGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCAGCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACa                                                                    >  1:2084516/1‑148 (MQ=255)
                                                                                 gggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACaa                                                                   >  2:2139308/1‑148 (MQ=255)
                                                                                 gggAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACaa                                                                   >  2:1956196/1‑148 (MQ=255)
                                                                                     aaaaTTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGt                                                               >  2:1256888/1‑148 (MQ=255)
                                                                                         ttGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTaa                                                           >  1:2336224/1‑148 (MQ=255)
                                                                                          tGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAg                                                          <  2:318125/148‑1 (MQ=255)
                                                                                          tGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAg                                                          <  1:1052607/148‑1 (MQ=255)
                                                                                            cGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGcc                                                        >  1:1011226/1‑148 (MQ=255)
                                                                                              gCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGt                                                      <  2:2000162/148‑1 (MQ=255)
                                                                                              gCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGt                                                      <  1:627396/148‑1 (MQ=255)
                                                                                              gCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGt                                                      <  1:2003974/148‑1 (MQ=255)
                                                                                               cATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGtt                                                     <  1:1261314/148‑1 (MQ=255)
                                                                                               cATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGtt                                                     >  2:2380179/1‑148 (MQ=255)
                                                                                               cATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGtt                                                     >  2:2439465/1‑148 (MQ=255)
                                                                                                  ttttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTCCGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCa                                                  >  2:2639085/1‑148 (MQ=255)
                                                                                                  ttttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCa                                                  >  2:330904/1‑148 (MQ=255)
                                                                                                   tttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCaa                                                 <  1:661892/148‑1 (MQ=255)
                                                                                                    ttCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCaaa                                                <  1:1200001/148‑1 (MQ=255)
                                                                                                        aGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGtt                                            >  1:2263189/1‑148 (MQ=255)
                                                                                                         gggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTc                                           <  1:655556/148‑1 (MQ=255)
                                                                                                         gggTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTc                                           >  1:3202067/1‑148 (MQ=255)
                                                                                                             cAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCttt                                       >  2:662658/1‑148 (MQ=255)
                                                                                                              aGAAAATCATAAAGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTc                                      <  1:2773919/148‑1 (MQ=255)
                                                                                                               gAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCa                                     <  2:946622/148‑1 (MQ=255)
                                                                                                                aaaaTCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCAc                                    >  1:1658549/1‑148 (MQ=255)
                                                                                                                        aaaGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCtt                            <  2:2596784/148‑1 (MQ=255)
                                                                                                                        aaaGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCtt                            >  1:2562716/1‑148 (MQ=255)
                                                                                                                          aGCCATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCAATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTg                          >  2:680567/1‑148 (MQ=255)
                                                                                                                          aGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTg                          >  2:1987719/1‑148 (MQ=255)
                                                                                                                            ccATTTGGTTTCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                        >  1:2400240/1‑148 (MQ=255)
                                                                                                                            ccATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                        >  2:2559834/1‑148 (MQ=255)
                                                                                                                            ccATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGcc                        >  2:3076122/1‑148 (MQ=255)
                                                                                                                              aTTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGt                      >  2:2280190/1‑148 (MQ=255)
                                                                                                                               tttGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTc                     >  2:1578385/1‑148 (MQ=255)
                                                                                                                               tttGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTc                     <  2:1240276/148‑1 (MQ=255)
                                                                                                                                  ggTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCttt                  >  2:1780776/1‑148 (MQ=255)
                                                                                                                                    tttCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGa                <  2:256785/148‑1 (MQ=255)
                                                                                                                                     ttCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGAc               >  1:3027384/1‑148 (MQ=255)
                                                                                                                                      tCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGAcc              >  2:2640330/1‑148 (MQ=255)
                                                                                                                                      tCAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGAcc              >  2:2447781/1‑148 (MQ=255)
                                                                                                                                       cAATATTATGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCa             >  1:2246996/1‑148 (MQ=255)
                                                                                                                                       cAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGTCCa             >  2:2731468/1‑148 (MQ=255)
                                                                                                                                         atatTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGGTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATc           >  2:2312287/1‑148 (MQ=255)
                                                                                                                                          tattatGCTAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCa          <  1:1310602/148‑1 (MQ=255)
                                                                                                                                          tattatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCa          >  2:2474444/1‑148 (MQ=255)
                                                                                                                                          tattatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCa          >  1:1410378/1‑148 (MQ=255)
                                                                                                                                          tattatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCa          >  1:1408774/1‑148 (MQ=255)
                                                                                                                                          tattatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCa          >  2:810774/1‑148 (MQ=255)
                                                                                                                                           attatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAg         >  1:1284580/1‑148 (MQ=255)
                                                                                                                                            ttatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGt        <  1:1780776/148‑1 (MQ=255)
                                                                                                                                             tatGCTAAATCGCACCACAGTCATCCCTTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGtt       >  1:2900796/1‑148 (MQ=255)
                                                                                                                                             tatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGtt       >  2:1395286/1‑148 (MQ=255)
                                                                                                                                             tatGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGtt       >  2:1608472/1‑148 (MQ=255)
                                                                                                                                                  aaaaTCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGTCTTTGCCGTCTTTGACCATCAGTTTGCcg  >  1:270667/1‑148 (MQ=255)
                                                                                                                                                  aaaaTCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGCcg  >  2:2400751/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATTCAACACCAATCGCCTGTTCCAGCGCACGGATGCGGCGGCTGAATGCCGGTTGCGAGACGTTGCGACTGACTGCCGCCTGGGAAAAATTGCGGCATTTTTCCAGGGTCAGAAAATCATAAAGCCATTTGGTTTCAATATTATGCAAAATCGCACCACAGTCATCCATTACGTTTTACTCTGTCAGCCGCCCTTGCGGGCATTCTACGTCCATTCGGGCGGCTGACAACCGTTTAAGCCGTTTCAAACGTTCCTTTCACGCAGGCTTTGCCGTCTTTGACCATCAGTTTGCCG  >  CP009273/4547938‑4548231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑