Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,585,338 A→T 100% S111T (TCT→ACT)  yjjM ← yjjMN operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,585,3380AT99.5% 678.6 / ‑4.6 205S111T (TCT→ACT) yjjMyjjMN operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (118/86);  total (118/87)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

GGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGT  >  CP009273/4585198‑4585481
                                                                                                                                            |                                                                                                                                               
ggCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGtt                                                                                                                                          <  1:1816428/148‑1 (MQ=255)
    tAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTccc                                                                                                                                      <  1:2962376/148‑1 (MQ=255)
    tAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTccc                                                                                                                                      >  1:1286889/1‑148 (MQ=255)
        aTTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTgg                                                                                                                                  >  1:2823881/1‑148 (MQ=255)
          tGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGc                                                                                                                                >  2:222187/1‑148 (MQ=255)
            aTCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGt                                                                                                                              >  2:2360422/1‑148 (MQ=255)
             tctaaTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTa                                                                                                                             <  1:2313289/145‑1 (MQ=255)
             tCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTa                                                                                                                             >  2:2353842/1‑148 (MQ=255)
             tCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTa                                                                                                                             <  1:2733381/148‑1 (MQ=255)
             tCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTa                                                                                                                             <  1:956252/148‑1 (MQ=255)
              cGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTaa                                                                                                                            <  2:2322316/148‑1 (MQ=255)
              cGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTaa                                                                                                                            <  2:3187072/148‑1 (MQ=255)
                aaTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACt                                                                                                                          >  1:3351436/1‑148 (MQ=255)
                aaTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACt                                                                                                                          >  1:132085/1‑148 (MQ=255)
                 aTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTg                                                                                                                         <  1:1373473/148‑1 (MQ=255)
                 aTTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTg                                                                                                                         <  1:228161/148‑1 (MQ=255)
                  ttGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTgg                                                                                                                        >  1:2779762/1‑148 (MQ=255)
                  ttGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTATCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTgg                                                                                                                        >  1:1730168/1‑148 (MQ=255)
                   tGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGgc                                                                                                                       >  1:1849442/1‑148 (MQ=255)
                     cTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGgcgc                                                                                                                     >  2:2014718/1‑148 (MQ=255)
                        cATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTgg                                                                                                                  <  2:2928605/148‑1 (MQ=255)
                              ccACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatc                                                                                                            <  2:303528/148‑1 (MQ=255)
                               cACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatca                                                                                                           <  2:866079/148‑1 (MQ=255)
                                  tGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCg                                                                                                        >  1:615341/1‑148 (MQ=255)
                                    gCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTg                                                                                                      <  1:2863346/148‑1 (MQ=255)
                                          cTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGaaaaaa                                                                                                >  1:1061689/1‑148 (MQ=255)
                                           ttttCGCTGTGAATAAGATTATCACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAg                                                                                               <  1:2884053/148‑1 (MQ=255)
                                           ttttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAg                                                                                               <  1:3173592/148‑1 (MQ=255)
                                            tttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGc                                                                                              <  1:2087302/148‑1 (MQ=255)
                                            tttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGc                                                                                              >  1:1902406/1‑148 (MQ=255)
                                             ttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                             <  1:1922048/148‑1 (MQ=255)
                                             ttCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc                                                                                             <  1:223450/148‑1 (MQ=255)
                                               cGCTGTTAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTg                                                                                           <  1:2999652/148‑1 (MQ=255)
                                               cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTg                                                                                           <  2:2872634/148‑1 (MQ=255)
                                               cGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTg                                                                                           <  1:750124/148‑1 (MQ=255)
                                                 cTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCt                                                                                         >  2:530780/1‑148 (MQ=255)
                                                    tgAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaa                                                                                      >  2:3486640/1‑148 (MQ=255)
                                                     gAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCaaa                                                                                     >  1:3240288/1‑148 (MQ=255)
                                                       aTAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAaca                                                                                   >  2:1404645/1‑148 (MQ=255)
                                                        tAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAacac                                                                                  <  2:1011346/148‑1 (MQ=255)
                                                        tAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAacac                                                                                  <  2:2629889/148‑1 (MQ=255)
                                                         aaGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACt                                                                                 <  2:1512425/148‑1 (MQ=255)
                                                            aTTATAACGGCCGAATTTTACAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAAAGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttg                                                                              <  1:1103208/148‑1 (MQ=255)
                                                            aTTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttg                                                                              >  1:2231946/1‑148 (MQ=255)
                                                              tataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt                                                                            >  2:3154645/1‑148 (MQ=255)
                                                               ataACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgttt                                                                           <  2:1985767/148‑1 (MQ=255)
                                                                      ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCAc                                                                    >  1:491503/1‑148 (MQ=255)
                                                                      ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTAGTTTTGTTCAc                                                                    >  2:1915391/1‑148 (MQ=255)
                                                                         aaTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCa                                                                 >  1:1139255/1‑148 (MQ=255)
                                                                          aTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCAt                                                                <  2:2420702/148‑1 (MQ=255)
                                                                          aTTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCAt                                                                >  2:417483/1‑148 (MQ=255)
                                                                           ttttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCAtt                                                               >  1:1236685/1‑148 (MQ=255)
                                                                           ttttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCAtt                                                               >  1:651197/1‑148 (MQ=255)
                                                                           ttttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCAtt                                                               >  1:2683771/1‑148 (MQ=255)
                                                                             ttAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGa                                                             <  1:3329352/148‑1 (MQ=255)
                                                                              tacaaGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGaa                                                            <  2:231553/145‑1 (MQ=255)
                                                                               aaaaGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAg                                                           <  1:1001659/148‑1 (MQ=255)
                                                                                  aGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCgg                                                        <  2:791317/148‑1 (MQ=255)
                                                                                   gATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgt                                                       <  2:895199/148‑1 (MQ=255)
                                                                                   gATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgt                                                       <  2:2185693/148‑1 (MQ=255)
                                                                                   gATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgt                                                       >  1:1350829/1‑148 (MQ=255)
                                                                                   gATATTCTCTTACGACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgt                                                       <  2:1808522/148‑1 (MQ=255)
                                                                                     tatTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtag                                                     >  1:3504281/1‑148 (MQ=255)
                                                                                     tatTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtag                                                     >  2:3342361/1‑148 (MQ=255)
                                                                                     tatTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGAAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTTTTCACTAATTGAAGCGgtag                                                     >  1:3018014/1‑148 (MQ=255)
                                                                                      atTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtagt                                                    >  1:1733114/1‑148 (MQ=255)
                                                                                       ttCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGgtagta                                                   >  1:338777/1‑148 (MQ=255)
                                                                                        tctcTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTAc                                                  >  1:610455/1‑148 (MQ=255)
                                                                                        tctcTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTAc                                                  >  1:965520/1‑148 (MQ=255)
                                                                                         ctctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACa                                                 >  1:1505519/1‑148 (MQ=255)
                                                                                         ctctTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACa                                                 >  1:1679541/1‑148 (MQ=255)
                                                                                            ttACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGc                                              >  1:1911026/1‑148 (MQ=255)
                                                                                             tactacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCg                                             >  2:3242194/1‑148 (MQ=255)
                                                                                             tactacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCg                                             >  2:1474768/1‑148 (MQ=255)
                                                                                                tacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa                                          >  1:1473393/1‑148 (MQ=255)
                                                                                                tacAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa                                          >  2:1116878/1‑148 (MQ=255)
                                                                                                 acAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAAc                                         >  1:2313629/1‑148 (MQ=255)
                                                                                                     ccGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtc                                     >  2:884887/1‑148 (MQ=255)
                                                                                                      cGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGtca                                    >  2:3320458/1‑148 (MQ=255)
                                                                                                            gACACCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATgg                              <  2:208379/148‑1 (MQ=255)
                                                                                                             acacCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGt                             >  1:2493794/1‑148 (MQ=255)
                                                                                                             acacCCGCGGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGt                             >  1:2387806/1‑148 (MQ=255)
                                                                                                                  cgcgGCCCGAGCCAGGTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGt                        <  1:60294/148‑1 (MQ=255)
                                                                                                                    cgGCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGttt                      <  2:1734475/148‑1 (MQ=255)
                                                                                                                      gCCCGAGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTAc                    >  1:3076220/1‑148 (MQ=255)
                                                                                                                           aGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCa               <  1:2133563/148‑1 (MQ=255)
                                                                                                                           aGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCa               >  1:582833/1‑148 (MQ=255)
                                                                                                                           aGCCAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCa               >  1:837002/1‑148 (MQ=255)
                                                                                                                             ccAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAAt             <  2:270265/148‑1 (MQ=255)
                                                                                                                             ccAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAAt             >  1:2346659/1‑148 (MQ=255)
                                                                                                                             ccAGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAAt             >  2:870773/1‑148 (MQ=255)
                                                                                                                               aGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGTCGAAACCGTCATCATGGTCAGGTTTACGCGCAATaa           >  2:2232121/1‑148 (MQ=255)
                                                                                                                               aGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATaa           <  1:2411160/148‑1 (MQ=255)
                                                                                                                               aGTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATaa           >  2:299308/1‑148 (MQ=255)
                                                                                                                                gTTGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAAc          >  2:1326659/1‑148 (MQ=255)
                                                                                                                                 ttGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACa         >  2:1248228/1‑148 (MQ=255)
                                                                                                                                  tGCAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACAt        >  1:77746/1‑148 (MQ=255)
                                                                                                                                    cAGTTCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAg      <  2:1932166/148‑1 (MQ=255)
                                                                                                                                       ttCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCg   >  2:1827700/1‑148 (MQ=255)
                                                                                                                                        tCAGTAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGt  <  1:1630620/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                               
GGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGT  >  CP009273/4585198‑4585481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑