BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,611,666	G→T	6.7%	A27A (GCG→GCT)	yjjJ →	putative protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,611,666	0	G	T	6.7%	889.8 / 23.4	238	A27A (GCG→GCT)	yjjJ	putative protein kinase
Reads supporting (aligned to +/- strand): ref base G (118/104); new base T (12/4); total (130/108)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 238 total aligned reads displayed.

GCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATT  >  CP009273/4611520‑4611812

gCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTAGTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGa                                                                                                                                                   <  1:1504529/148‑1 (MQ=255)

  tataCTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACg                                                                                                                                                 >  2:1680070/1‑148 (MQ=255)

     aCTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTtc                                                                                                                                              >  1:1847279/1‑148 (MQ=255)

     aCTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTtc                                                                                                                                              >  2:195550/1‑148 (MQ=255)

      cTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTtct                                                                                                                                             <  2:202805/148‑1 (MQ=255)

      cTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTtct                                                                                                                                             <  2:202391/148‑1 (MQ=255)

       ttttCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTtctc                                                                                                                                            <  1:260013/148‑1 (MQ=255)

       ttttCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTtctc                                                                                                                                            <  1:3540491/148‑1 (MQ=255)

         ttCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCAc                                                                                                                                          >  1:604613/1‑148 (MQ=255)

           cAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGc                                                                                                                                        >  2:892778/1‑148 (MQ=255)

            aGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGcc                                                                                                                                       >  2:2783773/1‑148 (MQ=255)

            aGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGcc                                                                                                                                       <  2:210189/148‑1 (MQ=255)

              tGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCtt                                                                                                                                     <  2:3181968/148‑1 (MQ=255)

               gAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttg                                                                                                                                    <  1:2685412/148‑1 (MQ=255)

                aaaTGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttgt                                                                                                                                   >  1:638290/1‑148 (MQ=255)

                 aaTGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTTATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttgtt                                                                                                                                  <  2:1469272/148‑1 (MQ=255)

                  aTGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCttgttg                                                                                                                                 >  2:140615/1‑148 (MQ=255)

                   tgtgTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGc                                                                                                                                >  1:43687/1‑148 (MQ=255)

                   tgtgTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGc                                                                                                                                >  1:1843697/1‑148 (MQ=255)

                       ttAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCaga                                                                                                                            >  1:1017976/1‑148 (MQ=255)

                       ttAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCaga                                                                                                                            >  1:1019060/1‑148 (MQ=255)

                        taataaATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCagag                                                                                                                           <  1:2182804/148‑1 (MQ=255)

                        taataaATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCagag                                                                                                                           <  1:2532374/148‑1 (MQ=255)

                         aataaATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAgaga                                                                                                                          >  2:1799585/1‑148 (MQ=255)

                           taaATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGagaag                                                                                                                        >  2:2371833/1‑148 (MQ=255)

                           taaATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGagaag                                                                                                                        >  2:588258/1‑148 (MQ=255)

                            aaaTCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGagaaga                                                                                                                       <  1:2112856/148‑1 (MQ=255)

                            aaaTCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGagaaga                                                                                                                       >  2:3056367/1‑148 (MQ=255)

                            aaaTCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGagaaga                                                                                                                       <  1:3459372/148‑1 (MQ=255)

                              ctCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATc                                                                                                                     <  2:3368703/147‑1 (MQ=255)

                               tCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCg                                                                                                                    <  1:936134/148‑1 (MQ=255)

                                 tATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCggg                                                                                                                  >  2:2045888/1‑148 (MQ=255)

                                   ttCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTg                                                                                                                <  1:2796708/148‑1 (MQ=255)

                                   ttCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTg                                                                                                                <  2:3005709/148‑1 (MQ=255)

                                       aGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTc                                                                                                            >  1:929976/1‑148 (MQ=255)

                                        gTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCg                                                                                                           <  2:3208571/148‑1 (MQ=255)

                                          aTCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCt                                                                                                         <  1:1473440/148‑1 (MQ=255)

                                            cTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCttt                                                                                                       >  2:3361263/1‑148 (MQ=255)

                                            cTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCttt                                                                                                       <  2:2524331/148‑1 (MQ=255)

                                               ttCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGt                                                                                                    >  2:1138913/1‑148 (MQ=255)

                                               ttCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGt                                                                                                    >  1:1715025/1‑148 (MQ=255)

                                                     aaTCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCa                                                                                              >  1:311486/1‑148 (MQ=255)

                                                          aTTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGc                                                                                         >  1:2061385/1‑148 (MQ=255)

                                                               ttAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAAcgc                                                                                    >  1:1630060/1‑148 (MQ=255)

                                                               ttAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAAcgc                                                                                    >  2:186987/1‑148 (MQ=255)

                                                                 aaTGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGa                                                                                  <  2:1831029/148‑1 (MQ=255)

                                                                  aTGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGat                                                                                 >  2:528187/1‑148 (MQ=255)

                                                                       cgagcTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCa                                                                            <  2:2455053/148‑1 (MQ=255)

                                                                          gcTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCActg                                                                         <  2:3481117/148‑1 (MQ=255)

                                                                           ctgactgaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCActgc                                                                        <  1:129791/148‑1 (MQ=255)

                                                                             gactgaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCActgctg                                                                      >  2:1076844/1‑148 (MQ=255)

                                                                              actgaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACtgctgc                                                                     >  2:750394/1‑148 (MQ=255)

                                                                                 gaTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTc                                                                  >  1:178714/1‑148 (MQ=255)

                                                                                  aTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTcc                                                                 >  1:2237013/1‑148 (MQ=255)

                                                                                  aTCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTcc                                                                 <  2:3487313/148‑1 (MQ=255)

                                                                                   tCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGTGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCt                                                                >  1:428849/1‑148 (MQ=255)

                                                                                    cTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCtt                                                               >  2:748146/1‑148 (MQ=255)

                                                                                    cTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCtt                                                               >  1:2716997/1‑148 (MQ=255)

                                                                                    cTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCtt                                                               >  2:447012/1‑148 (MQ=255)

                                                                                     ttttACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTa                                                              <  2:1843675/148‑1 (MQ=255)

                                                                                     ttttACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTa                                                              >  2:2611748/1‑148 (MQ=255)

                                                                                      tttACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTAt                                                             >  1:1746331/1‑148 (MQ=255)

                                                                                            gCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGa                                                       >  1:2321573/1‑148 (MQ=255)

                                                                                            gCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGa                                                       >  1:785875/1‑148 (MQ=255)

                                                                                            gCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGa                                                       >  1:2339581/1‑148 (MQ=255)

                                                                                             cAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGaa                                                      <  2:779710/148‑1 (MQ=255)

                                                                                               aGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCACCGCGATATGCACTGCTGCGTCCTTATCGCGGAAtt                                                    >  1:1690060/1‑148 (MQ=255)

                                                                                                gggCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTg                                                   >  1:538486/1‑148 (MQ=255)

                                                                                                gggCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTg                                                   >  2:3371801/1‑148 (MQ=255)

                                                                                                 ggCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGa                                                  <  1:2191458/148‑1 (MQ=255)

                                                                                                 ggCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGa                                                  >  1:1560293/1‑148 (MQ=255)

                                                                                                 ggCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGa                                                  <  1:1153129/148‑1 (MQ=255)

                                                                                                    cgcgTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCg                                               >  2:1232059/1‑148 (MQ=255)

                                                                                                      cgTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTa                                             >  2:3551444/1‑148 (MQ=255)

                                                                                                         tCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTc                                          >  1:35069/1‑148 (MQ=255)

                                                                                                         tCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTc                                          <  2:2403282/148‑1 (MQ=255)

                                                                                                             ccccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGt                                      <  2:2185641/148‑1 (MQ=255)

                                                                                                               ccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCt                                    >  1:1908028/1‑148 (MQ=255)

                                                                                                                  gAATTGCGCCAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGc                                 >  2:2788082/1‑148 (MQ=255)

                                                                                                                  gAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGc                                 <  1:1680070/148‑1 (MQ=255)

                                                                                                                     ttGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCggg                              <  2:1379126/148‑1 (MQ=255)

                                                                                                                        cgcCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTgg                           <  2:264733/148‑1 (MQ=255)

                                                                                                                          ccAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGAc                         >  2:2533582/1‑148 (MQ=255)

                                                                                                                           cAGCGTCTGGCAATCAGTCAGGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACg                        <  2:226428/148‑1 (MQ=255)

                                                                                                                             gCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGAt                      >  1:3066750/1‑148 (MQ=255)

                                                                                                                              cGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATa                     <  1:2652196/148‑1 (MQ=255)

                                                                                                                                      aaTCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAg             >  1:2802157/1‑148 (MQ=255)

                                                                                                                                      aaTCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAg             <  2:338568/148‑1 (MQ=255)

                                                                                                                                        tcagtcagGCTACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGgc           >  1:1971107/1‑148 (MQ=255)

                                                                                                                                        tcagtcagGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGgc           >  2:2672123/1‑148 (MQ=255)

                                                                                                                                             cagGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATa      <  1:3037072/148‑1 (MQ=255)

                                                                                                                                              agGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATaa     >  1:894998/1‑148 (MQ=255)

                                                                                                                                              agGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATaa     <  1:723281/148‑1 (MQ=255)

                                                                                                                                               ggCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATaaa    <  2:1748182/148‑1 (MQ=255)

                                                                                                                                               ggCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATaaa    <  2:929976/148‑1 (MQ=255)

                                                                                                                                                gCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAAt   <  1:2326942/148‑1 (MQ=255)

                                                                                                                                                gCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAAt   <  2:3055452/148‑1 (MQ=255)

                                                                                                                                                gCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAAt   <  2:3133626/148‑1 (MQ=255)

                                                                                                                                                 cGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAAtt  >  1:1315201/1‑148 (MQ=255)

                                                                                                                                                 cGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCGTTGGTAAAGCACGGGCAACGCGATATGCACAGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAAtt  >  1:2218407/1‑148 (MQ=255)

GCTATACTTTTCAGTGAAATGTGTTAATAAATCTATTCAAGTATCTATTCACGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATT  >  CP009273/4611520‑4611812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`