Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 435,862 A→T 93.1% T232T (ACT→ACA)  ispA ← geranyltranstransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273435,8620AT93.1% 535.6 / 22.5 197T232T (ACT→ACA) ispAgeranyltranstransferase
Reads supporting (aligned to +/- strand):  ref base A (9/5);  new base T (77/102);  total (89/108)
Fisher's exact test for biased strand distribution p-value = 1.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 197 total aligned reads displayed.

CTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAT  >  CP009273/435718‑436006
                                                                                                                                                |                                                                                                                                                
cTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTa                                                                                                                                               >  2:1355133/1‑148 (MQ=255)
cTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTa                                                                                                                                               >  2:3117745/1‑148 (MQ=255)
 tGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTAt                                                                                                                                              <  1:2063192/148‑1 (MQ=255)
  gTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTAtc                                                                                                                                             >  2:500059/1‑148 (MQ=255)
   ttCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCCACCTTGCTGTAtat                                                                                                                                            >  1:1072668/1‑146 (MQ=255)
           gttgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCcaccac                                                                                                                                    <  1:2934524/148‑1 (MQ=255)
             tgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTGCCAACGTTGCTGTATCTCCCACCACAt                                                                                                                                  >  2:1281615/1‑148 (MQ=255)
             tgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAt                                                                                                                                  <  2:363876/148‑1 (MQ=255)
             tgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAt                                                                                                                                  >  1:2854778/1‑148 (MQ=255)
             tgttTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCAGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAt                                                                                                                                  <  2:1455067/148‑1 (MQ=255)
                 tCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAg                                                                                                                              >  2:2063748/1‑148 (MQ=255)
                  cAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAgg                                                                                                                             <  1:2786556/148‑1 (MQ=255)
                  cAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAgg                                                                                                                             <  1:1011635/148‑1 (MQ=255)
                     cGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATg                                                                                                                          <  1:2284966/148‑1 (MQ=255)
                       aCTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTc                                                                                                                        <  1:3117745/148‑1 (MQ=255)
                           aCGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCAGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATcc                                                                                                                    >  1:1144535/1‑148 (MQ=255)
                            cGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCT‑CCACCACATCCAGGATGTCATCCTg                                                                                                                  <  2:2711309/148‑1 (MQ=255)
                              ggCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGa                                                                                                                 <  1:1530329/148‑1 (MQ=255)
                               gCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTTCTGTATATCCCACCACAACCAGGAATTCATCCAGaa                                                                                                                >  1:897864/1‑148 (MQ=255)
                               gCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGaa                                                                                                                <  1:1482764/148‑1 (MQ=255)
                               gCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGaa                                                                                                                >  2:194046/1‑148 (MQ=255)
                                     tcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGa                                                                                                          <  2:304436/148‑1 (MQ=255)
                                     tcgATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGa                                                                                                          >  2:2512564/1‑148 (MQ=255)
                                        aTCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaagg                                                                                                       <  2:2760393/148‑1 (MQ=255)
                                        aTCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaagg                                                                                                       <  2:3374971/148‑1 (MQ=255)
                                         tCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggc                                                                                                      >  1:1877241/1‑148 (MQ=255)
                                          cAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTGTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGACCCTGGaaggca                                                                                                     >  2:53185/1‑148 (MQ=255)
                                           aGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCTGTATCTACCACCACAACCAGGATGTCATCCAGAACCAGGaaggcaa                                                                                                    >  1:111867/1‑148 (MQ=255)
                                             aTCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaagg                                                                                                  <  1:2114962/148‑1 (MQ=255)
                                             aTCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaagg                                                                                                  <  2:1608551/148‑1 (MQ=255)
                                              tCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACCTCCAGGATGTCATCCTGAACCTGGaaggcaaggc                                                                                                 <  2:575847/148‑1 (MQ=255)
                                              tCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTGTTCCCCACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGaaggcaaggc                                                                                                 >  2:1215658/1‑148 (MQ=255)
                                                ccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCg                                                                                               <  2:333744/148‑1 (MQ=255)
                                                ccGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCg                                                                                               <  2:330962/148‑1 (MQ=255)
                                                  gggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGAt                                                                                             >  2:279225/1‑148 (MQ=255)
                                                   ggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATg                                                                                            <  1:186448/148‑1 (MQ=255)
                                                      tttCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGctc                                                                                         >  2:1135441/1‑148 (MQ=255)
                                                           tcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGGTGTATCAACCACCACATACACGATGTCAAGCTGAACCAGGAAGGCAAGGCCGATGCTCTCTGc                                                                                    >  1:1247848/1‑148 (MQ=255)
                                                            ccGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCa                                                                                   <  1:1701679/148‑1 (MQ=255)
                                                             cGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCAGAACCTGGAAGGCAAGGCCGATGCTCTCTGCAt                                                                                  >  1:489987/1‑148 (MQ=255)
                                                                gCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACt                                                                               >  1:2005860/1‑148 (MQ=255)
                                                                gCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAACCCCGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATaat                                                                               >  1:1912570/1‑146 (MQ=255)
                                                                  ttGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTGCCAACGTTGCTGTATCTCCCACCACATCCAGGGTGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTg                                                                             >  1:2159263/1‑148 (MQ=255)
                                                                  ttGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTg                                                                             >  2:1573532/1‑148 (MQ=255)
                                                                  ttGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTg                                                                             <  2:641802/148‑1 (MQ=255)
                                                                  ttGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCATGGCGTTTTGCCAACGTTTCTGTATATCCCACCACAAACACGATGTCATCCAGAAACAGGAAGTCAACGCCCATCCTCACTGCAAACtta                                                                             >  1:2159634/1‑147 (MQ=255)
                                                                   tGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGt                                                                            >  2:1716671/1‑148 (MQ=255)
                                                                     cTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCg                                                                          <  2:2224436/148‑1 (MQ=255)
                                                                      tCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGa                                                                         <  2:2996289/148‑1 (MQ=255)
                                                                         aGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTa                                                                      <  2:2656155/148‑1 (MQ=255)
                                                                         aGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTa                                                                      <  1:3206440/148‑1 (MQ=255)
                                                                         aGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCCGGATGTCATCCTGAACCTGGAAGGCCAGGCCGATGCTCTCTGCATACTTGTCGAGTa                                                                      >  1:2659232/1‑148 (MQ=255)
                                                                          gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                     >  2:1895597/1‑148 (MQ=255)
                                                                          gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCGGTATCTCCCACCACATACAGGATGTCATCCTGAAACAGGAAGGGAAGGCCGATGCTCTCTGCATACTTGTCGAGTaa                                                                     >  1:1523676/1‑147 (MQ=255)
                                                                          gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAAACAGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                     >  1:2508568/1‑148 (MQ=255)
                                                                          gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACCGTGCTGTATCTCCCACCACATCCAGGCTGTCCTCCGGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                     >  1:1613181/1‑148 (MQ=255)
                                                                               aGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGTCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCa                                                                <  2:2369947/148‑1 (MQ=255)
                                                                                 aaGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCACGATGTCATCCGGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACATTTCGAGTACCGGCaga                                                              >  1:165152/1‑148 (MQ=255)
                                                                                  aGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCagag                                                             <  1:1441880/148‑1 (MQ=255)
                                                                                      cAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGGTGTATCTCCCAACACATCCAGGATGTCATCAAGAACCAGGAAGGCAAGGCCGATGCTCTCTGCATAATTTTGGAGTACCGGCAGAGCacg                                                         >  1:751670/1‑148 (MQ=255)
                                                                                      cAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacg                                                         <  2:761900/148‑1 (MQ=255)
                                                                                       aGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacga                                                        <  1:1355133/148‑1 (MQ=255)
                                                                                         ggtaggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGGTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACGTGTCGAGTACCGGCAGAGCacgacg                                                      >  1:2609612/1‑148 (MQ=255)
                                                                                            aggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTcc                                                   >  2:2618667/1‑148 (MQ=255)
                                                                                              gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                 <  2:1206131/148‑1 (MQ=255)
                                                                                              gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCGGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCtt                                                 <  1:794094/148‑1 (MQ=255)
                                                                                              gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTGTTCCCAACGTTGCTGTATATCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATAATTGTCGAGTACCGGCCGAGCACGACGTCCtt                                                 >  1:3041544/1‑148 (MQ=255)
                                                                                                aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATTTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                               >  2:3140818/1‑148 (MQ=255)
                                                                                                aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                               >  2:1819107/1‑148 (MQ=255)
                                                                                                aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACAACCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                               >  1:206861/1‑148 (MQ=255)
                                                                                                 cTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAt                                              <  1:2630426/148‑1 (MQ=255)
                                                                                                 cTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAt                                              <  1:851003/148‑1 (MQ=255)
                                                                                                   tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtct                                            <  1:3354009/148‑1 (MQ=255)
                                                                                                    ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                           <  2:914833/148‑1 (MQ=255)
                                                                                                    ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                           <  2:1484805/148‑1 (MQ=255)
                                                                                                      aCCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATGCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTAGAGTAACGGCCGAGCACGACGTCCTTTATATCCg                                         >  1:3406409/1‑148 (MQ=255)
                                                                                                       ccAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCgg                                        >  2:88367/1‑148 (MQ=255)
                                                                                                        cAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCGTTATCTCCGgc                                       >  2:682943/1‑148 (MQ=255)
                                                                                                         aaGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgcg                                      <  2:1327179/148‑1 (MQ=255)
                                                                                                            ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCtt                                   >  2:2933828/1‑148 (MQ=255)
                                                                                                             tGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                  <  2:2191755/148‑1 (MQ=255)
                                                                                                             tGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTa                                  <  2:45716/148‑1 (MQ=255)
                                                                                                              gCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCCGAGCACGACGTCCTTTATCTCCGGCGCTTaa                                 >  2:1636018/1‑148 (MQ=255)
                                                                                                              gCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTaa                                 <  2:3319515/148‑1 (MQ=255)
                                                                                                               cTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAt                                <  2:2620742/148‑1 (MQ=255)
                                                                                                               cTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAAt                                >  1:2954441/1‑148 (MQ=255)
                                                                                                                 ggTCGGCACCCTGGCGTGTTCCCCACGTTGCTGTATCTCCCACCACATCCCGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACCACGTCCTTTATCTCCGGCGCTTAATGc                              >  2:620529/1‑148 (MQ=255)
                                                                                                                  gTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                             >  2:212225/1‑148 (MQ=255)
                                                                                                                  gTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                             >  2:1156497/1‑148 (MQ=255)
                                                                                                                     ggCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCa                          <  1:1229715/148‑1 (MQ=255)
                                                                                                                     ggCACCCTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCa                          <  1:521927/148‑1 (MQ=255)
                                                                                                                          ccTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCg                     <  1:1307262/148‑1 (MQ=255)
                                                                                                                           cTGGCGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGa                    <  2:2882281/148‑1 (MQ=255)
                                                                                                                               cGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcgg                >  1:3266428/1‑148 (MQ=255)
                                                                                                                               cGTTTTCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcgg                <  2:2903627/148‑1 (MQ=255)
                                                                                                                                    tCCCAACGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGNATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGgcggcg           <  1:1802761/148‑1 (MQ=255)
                                                                                                                                          cGTTGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATc     >  1:1568080/1‑148 (MQ=255)
                                                                                                                                             tGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt  >  1:1955625/1‑148 (MQ=255)
                                                                                                                                             tGCTGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGACAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt  >  2:2233948/1‑148 (MQ=255)
                                                                                                                                             tGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAt  <  1:2216874/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CTGTTCAGCCAGTTGTTTCAGCGACTGACGGGCATCGTCGATCAGATCCCGGGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAAT  >  CP009273/435718‑436006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑