Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 437,672 T→A 100% V223E (GTG→GAG)  thiI → tRNA s(4)U8 sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273437,6720TA99.5% 617.2 / ‑4.5 195V223E (GTG→GAG) thiItRNA s(4)U8 sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (91/103);  total (92/103)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

ACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGC  >  CP009273/437530‑437816
                                                                                                                                              |                                                                                                                                                
 cccAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTGCAGTTATATGTTGATTCGTCGCGGCTGCCGCGTGCATTACTGCTTCGTTAAACTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCa                                                                                                                                            >  1:2551021/1‑148 (MQ=255)
   cAGGAAGATGTGCTGTCGCTCCTTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCAtt                                                                                                                                          >  2:4173/1‑148 (MQ=255)
     ggAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTAt                                                                                                                                        <  1:931196/148‑1 (MQ=255)
      gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATTCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                       >  1:514574/1‑148 (MQ=255)
      gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATTCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCAGTATc                                                                                                                                       >  1:1726766/1‑148 (MQ=255)
      gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                       >  2:878954/1‑148 (MQ=255)
      gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATc                                                                                                                                       >  1:1760176/1‑148 (MQ=255)
        aGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTCTCtg                                                                                                                                     >  2:2882464/1‑148 (MQ=255)
        aGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCtg                                                                                                                                     <  1:473142/148‑1 (MQ=255)
        aGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCtg                                                                                                                                     >  1:2958959/1‑148 (MQ=255)
         gATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCtgt                                                                                                                                    >  1:2366397/1‑148 (MQ=255)
            gtgCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGa                                                                                                                                 <  2:2369929/148‑1 (MQ=255)
            gtgCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGa                                                                                                                                 <  1:3023742/148‑1 (MQ=255)
             tgCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGaa                                                                                                                                >  2:1452525/1‑148 (MQ=255)
                  tCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCt                                                                                                                           <  2:599722/148‑1 (MQ=255)
                   cGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCtt                                                                                                                          <  2:384070/148‑1 (MQ=255)
                   cGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCtt                                                                                                                          >  2:1130931/1‑148 (MQ=255)
                   cGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCtt                                                                                                                          >  2:1671873/1‑148 (MQ=255)
                      tCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTgg                                                                                                                       >  1:1076500/1‑148 (MQ=255)
                      tCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTgg                                                                                                                       >  1:2765036/1‑148 (MQ=255)
                       cATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGc                                                                                                                      >  2:7172/1‑148 (MQ=255)
                          ttCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGc                                                                                                                   <  2:1726607/148‑1 (MQ=255)
                             cGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTcc                                                                                                                <  1:2087170/148‑1 (MQ=255)
                                  gtTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACcg                                                                                                           >  1:3532375/1‑148 (MQ=255)
                                   tttCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACcgc                                                                                                          >  1:2944763/1‑148 (MQ=255)
                                        aCTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtgcg                                                                                                     >  2:3050413/1‑148 (MQ=255)
                                         cTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtgcgt                                                                                                    >  2:3089785/1‑148 (MQ=255)
                                         cTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtgcgt                                                                                                    <  1:1744711/148‑1 (MQ=255)
                                          tCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGtt                                                                                                   <  1:1238040/148‑1 (MQ=255)
                                          tCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGtt                                                                                                   >  1:1809939/1‑148 (MQ=255)
                                           ccGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCgcgtgcgtgt                                                                                                  >  2:2563902/1‑146 (MQ=255)
                                                gtTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCANGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGCACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCg                                                                                             <  1:1802527/148‑1 (MQ=255)
                                                 tttCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGc                                                                                            >  2:1144446/1‑148 (MQ=255)
                                                        ttATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAt                                                                                     >  1:2715366/1‑148 (MQ=255)
                                                         tataTGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAtt                                                                                    <  1:7172/148‑1 (MQ=255)
                                                          atatGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                   <  2:3548968/148‑1 (MQ=255)
                                                          atatGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                   <  2:2185886/148‑1 (MQ=255)
                                                          atatGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAAttt                                                                                   <  1:500506/148‑1 (MQ=255)
                                                            atGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCg                                                                                 >  1:494664/1‑148 (MQ=255)
                                                             tGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGa                                                                                <  1:118756/148‑1 (MQ=255)
                                                             tGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGa                                                                                <  1:2939694/148‑1 (MQ=255)
                                                              gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGaa                                                                               <  1:2843452/148‑1 (MQ=255)
                                                               ttGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAc                                                                              <  2:967973/148‑1 (MQ=255)
                                                                 gATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCg                                                                            <  1:1604774/148‑1 (MQ=255)
                                                                  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCgg                                                                           >  2:1697198/1‑148 (MQ=255)
                                                                  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCgg                                                                           >  2:1615206/1‑148 (MQ=255)
                                                                  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCgg                                                                           <  1:1671873/148‑1 (MQ=255)
                                                                  aTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCgg                                                                           <  1:647319/148‑1 (MQ=255)
                                                                    gCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGgtc                                                                         <  2:936947/148‑1 (MQ=255)
                                                                        cgcgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGtcgtcg                                                                     <  1:1025763/148‑1 (MQ=255)
                                                                         gcgGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgg                                                                    <  2:514574/148‑1 (MQ=255)
                                                                           ggCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgggg                                                                  <  2:1976747/148‑1 (MQ=255)
                                                                           ggCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCgggg                                                                  <  2:1294934/148‑1 (MQ=255)
                                                                                ccGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAAtt                                                             >  2:2922266/1‑148 (MQ=255)
                                                                                ccGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAAtt                                                             >  1:57858/1‑148 (MQ=255)
                                                                                 cgcgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATtc                                                            <  1:1859037/148‑1 (MQ=255)
                                                                                     tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCga                                                        <  1:1458867/148‑1 (MQ=255)
                                                                                     tGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCga                                                        <  1:878954/148‑1 (MQ=255)
                                                                                      gCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCgag                                                       >  2:2345530/1‑148 (MQ=255)
                                                                                      gCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCgag                                                       >  2:2345516/1‑148 (MQ=255)
                                                                                       cATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCgaga                                                      >  2:1505874/1‑148 (MQ=255)
                                                                                        aTTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaa                                                     <  1:1329557/148‑1 (MQ=255)
                                                                                         ttACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaaa                                                    >  2:2120470/1‑148 (MQ=255)
                                                                                          tACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaaaa                                                   <  1:39038/148‑1 (MQ=255)
                                                                                          tACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGaaaa                                                   >  2:2505336/1‑148 (MQ=255)
                                                                                           aCTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAAt                                                  <  2:2762307/148‑1 (MQ=255)
                                                                                             tGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcg                                                >  1:298756/1‑148 (MQ=255)
                                                                                             tGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcg                                                >  1:2093698/1‑148 (MQ=255)
                                                                                               cttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgac                                              <  2:3224426/148‑1 (MQ=255)
                                                                                                ttcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacg                                             >  1:2252077/1‑148 (MQ=255)
                                                                                                 tcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacga                                            <  2:2555378/148‑1 (MQ=255)
                                                                                                 tcttTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATcgacga                                            <  2:1031770/148‑1 (MQ=255)
                                                                                                   tttAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGacgacg                                          >  1:617862/1‑148 (MQ=255)
                                                                                                     tAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGt                                        <  2:3394925/148‑1 (MQ=255)
                                                                                                        ccTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAg                                     >  1:859678/1‑148 (MQ=255)
                                                                                                           cggcggCGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCTTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATg                                  <  1:1008094/148‑1 (MQ=255)
                                                                                                            ggcggcGCGGCGCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCGGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATgg                                 <  1:1430931/148‑1 (MQ=255)
                                                                                                             gcggcgcggcGCATGAAATTGGCGTGCGTCAGGAGGCGAATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATggg                                <  1:3117469/148‑1 (MQ=255)
                                                                                                              cggcgcggcgCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGc                               >  2:2575841/1‑148 (MQ=255)
                                                                                                              cggcgcggcgCATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGc                               >  2:3098055/1‑148 (MQ=255)
                                                                                                                   cggcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTAt                          <  2:530447/148‑1 (MQ=255)
                                                                                                                     gcgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATcc                        <  2:1072300/148‑1 (MQ=255)
                                                                                                                      cgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCt                       <  2:1782890/148‑1 (MQ=255)
                                                                                                                      cgcATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCt                       <  1:789152/148‑1 (MQ=255)
                                                                                                                        cATGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAACTTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCa                     >  2:1669446/1‑148 (MQ=255)
                                                                                                                         aTGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                    >  1:437426/1‑148 (MQ=255)
                                                                                                                         aTGAAATTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                    <  1:2593602/148‑1 (MQ=255)
                                                                                                                             aaTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGt                <  2:2918444/148‑1 (MQ=255)
                                                                                                                              aTTGGCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTa               >  1:3434676/1‑148 (MQ=255)
                                                                                                                                 ggCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatga            <  1:1738228/148‑1 (MQ=255)
                                                                                                                                 ggCGTGCGTCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatga            <  1:1738329/148‑1 (MQ=255)
                                                                                                                                  gcgtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatgat           >  2:1909930/1‑148 (MQ=255)
                                                                                                                                  gcgtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCGCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatgat           <  2:1388459/148‑1 (MQ=255)
                                                                                                                                   cgtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTatgatg          <  1:2882464/148‑1 (MQ=255)
                                                                                                                                    gtgcgtCAGGAGGCGCTTTATCTGTGCAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGCCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGATAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATgg         <  1:1328479/148‑1 (MQ=255)
                                                                                                                                    gtgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCTTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGTAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATgg         <  1:4108/148‑1 (MQ=255)
                                                                                                                                     tgcgtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgt        >  1:839810/1‑148 (MQ=255)
                                                                                                                                        gtCAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcg     <  1:1489269/148‑1 (MQ=255)
                                                                                                                                          cAGGAGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcgtg   >  1:1137588/1‑148 (MQ=255)
                                                                                                                                           aggaggCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGgtgcgtgc  >  1:3288488/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                
ACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGC  >  CP009273/437530‑437816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑