Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 456,588 G→T 100% D749Y (GAC→TAC)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273456,5880GT100.0% 552.7 / NA 167D749Y (GAC→TAC) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (76/91);  total (76/91)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

GTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGG  >  CP009273/456444‑456731
                                                                                                                                                |                                                                                                                                               
gtgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACa                                                                                                                                              >  2:1167909/1‑148 (MQ=255)
 tgGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACaa                                                                                                                                             <  1:3335910/148‑1 (MQ=255)
   gCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACg                                                                                                                                           >  2:265842/1‑148 (MQ=255)
      aTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTaa                                                                                                                                        <  1:752948/148‑1 (MQ=255)
      aTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTaa                                                                                                                                        <  1:2950473/148‑1 (MQ=255)
      aTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTaa                                                                                                                                        <  2:2922135/148‑1 (MQ=255)
      aTGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTaa                                                                                                                                        <  2:3235621/148‑1 (MQ=255)
             gTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGa                                                                                                                                 <  1:721798/148‑1 (MQ=255)
             gTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGa                                                                                                                                 >  1:324257/1‑148 (MQ=255)
              tGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGAt                                                                                                                                <  2:2204942/148‑1 (MQ=255)
              tGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGAt                                                                                                                                >  2:799356/1‑148 (MQ=255)
                 gaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTg                                                                                                                             <  2:517524/148‑1 (MQ=255)
                 gaTCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTg                                                                                                                             <  2:518473/148‑1 (MQ=255)
                   tCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGa                                                                                                                           <  1:1157182/148‑1 (MQ=255)
                    cACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGAc                                                                                                                          <  1:797143/148‑1 (MQ=255)
                    cACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGAc                                                                                                                          >  2:354930/1‑148 (MQ=255)
                     aCTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACa                                                                                                                         >  2:1432099/1‑148 (MQ=255)
                      ctctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGACAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACAt                                                                                                                        <  2:372141/148‑1 (MQ=255)
                        ctGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTc                                                                                                                      <  1:2454935/148‑1 (MQ=255)
                         tGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCa                                                                                                                     <  2:1293982/148‑1 (MQ=255)
                             tGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCt                                                                                                                 <  2:2398086/148‑1 (MQ=255)
                               gTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCtgt                                                                                                               <  2:226840/148‑1 (MQ=255)
                                 cAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGa                                                                                                             >  2:876796/1‑148 (MQ=255)
                                  aGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGaa                                                                                                            <  2:2624872/148‑1 (MQ=255)
                                       cTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCa                                                                                                       <  1:2125664/148‑1 (MQ=255)
                                              tCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                <  2:189772/148‑1 (MQ=255)
                                              tCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                <  2:166702/148‑1 (MQ=255)
                                              tCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                <  2:189818/148‑1 (MQ=255)
                                              tCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAAGTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                <  2:1484830/148‑1 (MQ=255)
                                              tCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAAGTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGa                                                                                                <  2:655833/148‑1 (MQ=255)
                                               cGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGaa                                                                                               <  2:2615820/148‑1 (MQ=255)
                                                ggtggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAg                                                                                              <  2:2660129/148‑1 (MQ=255)
                                                ggtggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAg                                                                                              <  1:199248/148‑1 (MQ=255)
                                                  tggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGtt                                                                                            >  2:2771744/1‑148 (MQ=255)
                                                  tggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCACATTGAGGAAGtt                                                                                            >  1:252136/1‑148 (MQ=255)
                                                   ggtTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTc                                                                                           <  2:1698947/148‑1 (MQ=255)
                                                     tttGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTg                                                                                         >  2:341022/1‑148 (MQ=255)
                                                        gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAct                                                                                      <  1:235127/148‑1 (MQ=255)
                                                        gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAct                                                                                      <  1:3159163/148‑1 (MQ=255)
                                                        gaaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGAct                                                                                      <  1:862988/148‑1 (MQ=255)
                                                         aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActc                                                                                     <  1:3235300/148‑1 (MQ=255)
                                                         aaagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActc                                                                                     <  2:227284/148‑1 (MQ=255)
                                                          aagaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGActct                                                                                    >  2:802487/1‑148 (MQ=255)
                                                           agaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTg                                                                                   >  1:108920/1‑148 (MQ=255)
                                                            gaaaAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATAACGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGAAATTGCCGATCTGGACATTCATCCTGAGAAGCTCATTGAGGAAGTACTGACTCTgg                                                                                  >  2:1602743/1‑148 (MQ=255)
                                                             aaaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgc                                                                                 >  1:374714/1‑148 (MQ=255)
                                                              aaaaCTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGgcg                                                                                <  1:2730744/148‑1 (MQ=255)
                                                                  cTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGc                                                                            >  1:879380/1‑148 (MQ=255)
                                                                   tCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAACATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCa                                                                           >  1:467912/1‑148 (MQ=255)
                                                                    ccTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaa                                                                          >  1:920470/1‑148 (MQ=255)
                                                                      tGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCaaaa                                                                        <  2:3228352/148‑1 (MQ=255)
                                                                         cAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGa                                                                     <  2:2960223/148‑1 (MQ=255)
                                                                          aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGaa                                                                    <  2:713942/148‑1 (MQ=255)
                                                                          aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGaa                                                                    <  2:2686993/148‑1 (MQ=255)
                                                                          aGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGaa                                                                    <  2:2513369/148‑1 (MQ=255)
                                                                           gcgcATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAAc                                                                   >  1:91324/1‑148 (MQ=255)
                                                                           gcgcATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAAc                                                                   >  1:3462713/1‑148 (MQ=255)
                                                                            cgcATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAAcc                                                                  >  2:3138850/1‑148 (MQ=255)
                                                                                tCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCt                                                              <  2:938950/148‑1 (MQ=255)
                                                                                 cgcgGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTg                                                             >  2:3219135/1‑148 (MQ=255)
                                                                                  gcggcgGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTgg                                                            <  2:3250164/148‑1 (MQ=255)
                                                                                  gcggcgGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTgg                                                            <  1:727060/148‑1 (MQ=255)
                                                                                   cggcggGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGt                                                           <  1:192979/148‑1 (MQ=255)
                                                                                     gcggGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTAt                                                         >  1:2558283/1‑148 (MQ=255)
                                                                                        ggATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCa                                                      <  2:473327/148‑1 (MQ=255)
                                                                                            tAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGtt                                                  >  2:1098609/1‑148 (MQ=255)
                                                                                             aaaaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtg                                                 <  2:2990384/148‑1 (MQ=255)
                                                                                               aaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgtg                                               >  1:1350907/1‑148 (MQ=255)
                                                                                               aaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgtg                                               >  1:178450/1‑148 (MQ=255)
                                                                                               aaCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTtgtg                                               >  1:352162/1‑148 (MQ=255)
                                                                                                aCAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGa                                              >  2:3015821/1‑148 (MQ=255)
                                                                                                  aGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACt                                            >  1:1825245/1‑148 (MQ=255)
                                                                                                     gCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCa                                         >  2:649744/1‑148 (MQ=255)
                                                                                                     gCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCa                                         <  2:2017692/148‑1 (MQ=255)
                                                                                                       tAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaa                                       <  2:1226026/148‑1 (MQ=255)
                                                                                                       tAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCaaa                                       <  1:2525094/148‑1 (MQ=255)
                                                                                                          ttCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCACAATa                                    >  2:2755206/1‑148 (MQ=255)
                                                                                                           tCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAg                                   >  1:2804645/1‑148 (MQ=255)
                                                                                                           tCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCAGTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAg                                   >  2:1838487/1‑148 (MQ=255)
                                                                                                              gTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGa                                <  1:3507232/148‑1 (MQ=255)
                                                                                                              gTTCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGa                                >  1:1059252/1‑148 (MQ=255)
                                                                                                               ttCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAc                               <  2:2934045/148‑1 (MQ=255)
                                                                                                               ttCGAAAATAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGAc                               <  2:1832250/148‑1 (MQ=255)
                                                                                                                   aaaaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcg                           <  1:2614588/148‑1 (MQ=255)
                                                                                                                    aaaTAAACGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgc                          >  1:3010177/1‑148 (MQ=255)
                                                                                                                          aCGCGATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaaaa                    <  1:2712529/148‑1 (MQ=255)
                                                                                                                             cgATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGc                 >  1:1437107/1‑148 (MQ=255)
                                                                                                                             cgATCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGc                 >  2:283114/1‑148 (MQ=255)
                                                                                                                               aTCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCACAATGCAc               >  2:1932998/1‑148 (MQ=255)
                                                                                                                                tCTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACt              <  1:359677/148‑1 (MQ=255)
                                                                                                                                 cTGGAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACta             <  2:1184611/148‑1 (MQ=255)
                                                                                                                                   ggAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaat           <  1:1648616/148‑1 (MQ=255)
                                                                                                                                    gAAGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata          >  1:2064508/1‑148 (MQ=255)
                                                                                                                                     aaGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaataa         >  2:3289351/1‑148 (MQ=255)
                                                                                                                                     aaGAGATTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaataa         >  1:2540418/1‑148 (MQ=255)
                                                                                                                                       gagaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaa       <  2:334796/148‑1 (MQ=255)
                                                                                                                                       gagaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaa       >  1:2132069/1‑148 (MQ=255)
                                                                                                                                       gagaTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaa       >  2:179301/1‑148 (MQ=255)
                                                                                                                                          aTTCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACa    >  1:2045358/1‑148 (MQ=255)
                                                                                                                                           ttCCTTACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCACAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAg   >  2:1937480/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GTGGCAATGACCGGTGAGATCACTCTGCGTGGTCAGGTACTGCCGATCGGTGGTTTGAAAGAAAAACTCCTGGCAGCGCATCGCGGCGGGATTAAAACAGTGCTAATTCCGTTCGAAAATAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGG  >  CP009273/456444‑456731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑