Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 461,499 G→T 100% R424S (CGC→AGC)  ybaE ← putative ABC superfamily transporter periplasmic‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273461,4990GT98.0% 691.6 / 3.4 220R424S (CGC→AGC) ybaEputative ABC superfamily transporter periplasmic‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (95/120);  total (98/122)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 224 total aligned reads displayed.

AGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAC  >  CP009273/461354‑461643
                                                                                                                                                 |                                                                                                                                                
agagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCttt                                                                                                                                                >  2:439308/1‑148 (MQ=255)
agagTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTAGTCCCAGTATTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCttt                                                                                                                                                <  2:251949/148‑1 (MQ=255)
  agTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCt                                                                                                                                              >  2:737679/1‑148 (MQ=255)
  agTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCt                                                                                                                                              >  2:618942/1‑148 (MQ=255)
  agTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCt                                                                                                                                              <  1:2302859/148‑1 (MQ=255)
   gTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTg                                                                                                                                             >  1:1451294/1‑148 (MQ=255)
    tataTTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGc                                                                                                                                            <  1:420796/148‑1 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                          <  2:644539/148‑1 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                          <  1:1969206/148‑1 (MQ=255)
      tatTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCa                                                                                                                                          <  2:1577112/148‑1 (MQ=255)
           cGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTa                                                                                                                                     <  2:2175681/148‑1 (MQ=255)
             gTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATg                                                                                                                                   >  2:2425868/1‑148 (MQ=255)
             gTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATg                                                                                                                                   >  2:3477787/1‑148 (MQ=255)
              tGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGa                                                                                                                                  >  2:3514373/1‑148 (MQ=255)
               gCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGaa                                                                                                                                 >  1:806945/1‑148 (MQ=255)
                cTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAg                                                                                                                                >  2:2780173/1‑148 (MQ=255)
                 ttCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGt                                                                                                                               <  2:981292/148‑1 (MQ=255)
                 ttCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGt                                                                                                                               <  1:2178578/148‑1 (MQ=255)
                 ttCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGt                                                                                                                               <  2:981260/148‑1 (MQ=255)
                  tCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGtt                                                                                                                              <  2:2479084/148‑1 (MQ=255)
                  tCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGtt                                                                                                                              <  2:413221/148‑1 (MQ=255)
                    gCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCt                                                                                                                            <  1:1712170/148‑1 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                           <  2:232121/148‑1 (MQ=255)
                     ccAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTa                                                                                                                           >  1:1117437/1‑148 (MQ=255)
                      cAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTAt                                                                                                                          <  1:262236/148‑1 (MQ=255)
                        aTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCg                                                                                                                        >  2:2922270/1‑148 (MQ=255)
                        aTTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCg                                                                                                                        >  2:1150103/1‑148 (MQ=255)
                         ttAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCgg                                                                                                                       >  2:2021183/1‑148 (MQ=255)
                         ttAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCgg                                                                                                                       >  1:1689687/1‑148 (MQ=255)
                          tAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGt                                                                                                                      >  1:2263547/1‑148 (MQ=255)
                          tAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGt                                                                                                                      <  1:3447431/148‑1 (MQ=255)
                            aTCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAg                                                                                                                    >  1:68229/1‑148 (MQ=255)
                               tGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTg                                                                                                                 <  2:3375430/148‑1 (MQ=255)
                                gTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGa                                                                                                                <  2:890248/148‑1 (MQ=255)
                                 tCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGAt                                                                                                               <  1:1268996/148‑1 (MQ=255)
                                       atcatGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCa                                                                                                         <  1:2071825/148‑1 (MQ=255)
                                         catGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGc                                                                                                       >  2:2450081/1‑148 (MQ=255)
                                           tGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGt                                                                                                     >  1:1585208/1‑148 (MQ=255)
                                                tCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAgg                                                                                                <  2:1709925/148‑1 (MQ=255)
                                                tCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAgg                                                                                                <  1:979354/148‑1 (MQ=255)
                                                   gCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGttt                                                                                             <  2:1353605/148‑1 (MQ=255)
                                                     gtgtGCGTGTAGGTTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTc                                                                                           <  2:3529494/148‑1 (MQ=255)
                                                        tgCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGt                                                                                        <  1:1152505/148‑1 (MQ=255)
                                                          ccTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCAAAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAg                                                                                      >  2:1471977/1‑148 (MQ=255)
                                                           cTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCTTGTAGGGGTTCTTCCATTGTATTAAATTCTATAGGTAGGTTATAAACCAGCGTCAAGGTTTTCGGTAGt                                                                                     >  1:3057717/1‑148 (MQ=255)
                                                                    cgtgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCa                                                                            <  1:2950419/148‑1 (MQ=255)
                                                                     gtgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCAt                                                                           <  2:401522/148‑1 (MQ=255)
                                                                     gtgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCAt                                                                           <  1:2209330/148‑1 (MQ=255)
                                                                      tgtcgtCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATc                                                                          <  1:490555/148‑1 (MQ=255)
                                                                         cgtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCgg                                                                       <  1:2156970/148‑1 (MQ=255)
                                                                          gtcCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGt                                                                      <  2:2045649/148‑1 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAc                                                                    <  1:3103339/148‑1 (MQ=255)
                                                                            cccAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTAc                                                                    >  1:3338168/1‑148 (MQ=255)
                                                                              cAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCt                                                                  >  1:1819826/1‑148 (MQ=255)
                                                                               aGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTg                                                                 >  2:3161993/1‑148 (MQ=255)
                                                                               aGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTg                                                                 <  1:2412288/148‑1 (MQ=255)
                                                                                    tttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAAt                                                            <  2:2275066/148‑1 (MQ=255)
                                                                                     ttGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATg                                                           >  2:13961/1‑148 (MQ=255)
                                                                                      tGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGc                                                          <  2:38765/148‑1 (MQ=255)
                                                                                       gCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCg                                                         <  1:3503934/148‑1 (MQ=255)
                                                                                             tGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAg                                                   <  2:1781421/148‑1 (MQ=255)
                                                                                             tGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAg                                                   >  1:1432599/1‑148 (MQ=255)
                                                                                              gAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGACTAGt                                                  >  1:197565/1‑148 (MQ=255)
                                                                                              gAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGt                                                  <  2:2930687/148‑1 (MQ=255)
                                                                                              gAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGt                                                  <  1:1688557/148‑1 (MQ=255)
                                                                                              gAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGt                                                  >  2:2834188/1‑148 (MQ=255)
                                                                                              gAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGt                                                  >  2:2832879/1‑148 (MQ=255)
                                                                                                 aaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCa                                               >  2:1595292/1‑148 (MQ=255)
                                                                                                 aaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCa                                               >  1:3402147/1‑148 (MQ=255)
                                                                                                 aaaTAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCa                                               >  2:2308042/1‑148 (MQ=255)
                                                                                                   ataatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGc                                             >  2:150337/1‑148 (MQ=255)
                                                                                                     aatTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCt                                           >  2:2198455/1‑148 (MQ=255)
                                                                                                      atTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTg                                          >  1:3181622/1‑148 (MQ=255)
                                                                                                      atTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTg                                          >  1:396494/1‑148 (MQ=255)
                                                                                                       ttGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTgg                                         >  1:851523/1‑148 (MQ=255)
                                                                                                        tgtgAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGACTAGTCCAGCCTGGc                                        >  2:3355641/1‑148 (MQ=255)
                                                                                                           gAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGt                                     >  2:2186420/1‑148 (MQ=255)
                                                                                                           gAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGt                                     <  2:1033709/148‑1 (MQ=255)
                                                                                                             gTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTaa                                   >  1:3420877/1‑148 (MQ=255)
                                                                                                              ttCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAAt                                  >  1:577662/1‑148 (MQ=255)
                                                                                                                cacaGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGc                                >  1:1007165/1‑148 (MQ=255)
                                                                                                                cacaGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGc                                <  1:1542860/148‑1 (MQ=255)
                                                                                                                cacaGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGc                                <  2:1183557/148‑1 (MQ=255)
                                                                                                                  caGCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCAt                              >  1:2987782/1‑148 (MQ=255)
                                                                                                                    gCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGa                            <  2:476169/148‑1 (MQ=255)
                                                                                                                    gCCTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGa                            <  1:2780173/148‑1 (MQ=255)
                                                                                                                     ccTTCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGAc                           >  2:943222/1‑148 (MQ=255)
                                                                                                                       ttCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTg                         >  1:2370872/1‑148 (MQ=255)
                                                                                                                       ttCCGCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTg                         >  2:526511/1‑148 (MQ=255)
                                                                                                                           gCTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCgg                     <  2:3038980/148‑1 (MQ=255)
                                                                                                                            cTGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGt                    >  1:890494/1‑148 (MQ=255)
                                                                                                                             tGCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTg                   <  2:2552678/148‑1 (MQ=255)
                                                                                                                              gCCAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGa                  >  1:2917253/1‑148 (MQ=255)
                                                                                                                                cAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATc                >  1:1627543/1‑148 (MQ=255)
                                                                                                                                cAGTGTCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATc                >  2:1809153/1‑148 (MQ=255)
                                                                                                                                   tgtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAgg             <  1:373654/148‑1 (MQ=255)
                                                                                                                                    gtCGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGt            <  1:622905/148‑1 (MQ=255)
                                                                                                                                      cGCCTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGttt          <  1:446869/148‑1 (MQ=255)
                                                                                                                                         cTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtct       >  1:472345/1‑148 (MQ=255)
                                                                                                                                         cTGTAGGCTTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTtct       <  1:1290584/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGGTACCTGCCAATGCGGAATAGTCCAGCCTGGCAGTAATGCATGACTGGCGGTGATCAGGTTTTCTCCGAC  >  CP009273/461354‑461643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑