| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 482,757 | G→T | 91.7% | A256S (GCG→TCG) | mscK → | mechanosensitive channel protein, intermediate conductance, K+ regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 482,757 | 0 | G | T | 91.7% | 505.8 / 39.4 | 193 | A256S (GCG→TCG) | mscK | mechanosensitive channel protein, intermediate conductance, K+ regulated |
| Reads supporting (aligned to +/- strand): ref base G (7/9); new base T (90/87); total (97/96) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.13e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
Only 100 of 195 total aligned reads displayed.
AGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC > CP009273/482614‑482900 | agGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGt < 2:824336/148‑1 (MQ=255) gTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAAc < 1:1029076/148‑1 (MQ=255) tGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAg > 1:2275201/1‑148 (MQ=255) cTGAATGCTGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAAAGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCa < 1:3208680/148‑1 (MQ=255) cTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCa < 1:2632211/148‑1 (MQ=255) cTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCa < 2:1570014/148‑1 (MQ=255) cTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCa < 2:3501202/148‑1 (MQ=255) cTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGAGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACATCa < 1:1075489/148‑1 (MQ=255) tGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAgcgc > 2:2936994/1‑148 (MQ=255) gCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGcc < 2:238934/148‑1 (MQ=255) gaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATAGGTAAACAGCAAGCGCCTGACt < 2:427323/148‑1 (MQ=255) gaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACt < 1:2064546/148‑1 (MQ=255) tGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTa > 1:3020164/1‑148 (MQ=255) tGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATAGGTAAACAGCAAGCGCCTGACTTTa < 1:3019733/148‑1 (MQ=255) gACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTaa < 2:625798/148‑1 (MQ=255) cagcagCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTACACAGCAAGCGCCTGACTTTAACCg > 2:2162685/1‑148 (MQ=255) cagcGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaa > 2:817010/1‑148 (MQ=255) gcGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaa < 2:2779667/148‑1 (MQ=255) gcGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaa < 2:74821/148‑1 (MQ=255) cGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGaaaaaa > 1:783130/1‑148 (MQ=255) aaaaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACgg > 2:1305522/1‑148 (MQ=255) aaaaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACgg > 1:2197639/1‑148 (MQ=255) aaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGgcg > 1:1492471/1‑148 (MQ=255) aGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGgcgc > 1:2332664/1‑148 (MQ=255) aGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGgcgc > 1:348643/1‑148 (MQ=255) gCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCCGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCa < 1:251857/148‑1 (MQ=255) gCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCa > 2:296668/1‑148 (MQ=255) ccTGGAAGGGAACACCTTCTTGCTGGATACCTTGAAAAAGAAACGTTATTAGGTGACGGCGAACAGCGCTCGTCTGGAGAACCTGTTAAAAGTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAg < 1:180497/148‑1 (MQ=255) ccTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAg < 2:3126012/148‑1 (MQ=255) tGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGa > 1:1731293/1‑148 (MQ=255) gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg < 2:289718/148‑1 (MQ=255) gAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAg < 2:2872629/148‑1 (MQ=255) aaGGTAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGc < 1:2921417/148‑1 (MQ=255) aaGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGc > 2:309917/1‑148 (MQ=255) aaGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGc > 2:25075/1‑148 (MQ=255) aaGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGc < 2:3446290/148‑1 (MQ=255) ggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt < 1:3199476/148‑1 (MQ=255) ggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt > 2:1813856/1‑148 (MQ=255) ggAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGt > 2:1813512/1‑148 (MQ=255) gAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACAGAAATAACGCCGCAGGAAGCCGtc > 1:2044908/1‑148 (MQ=255) acacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAATCGCCTGACTTTAACCGAAAAAACGGCGCCGGAAGCCGtctc > 1:2425460/1‑148 (MQ=255) acacCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGtctc > 2:3057707/1‑148 (MQ=255) ccGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTcccc > 1:1841820/1‑148 (MQ=255) ccGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTcccc > 1:436752/1‑148 (MQ=255) ggATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGcc > 1:1323773/1‑148 (MQ=255) gATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg > 2:539627/1‑148 (MQ=255) gATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCcg > 1:3403196/1‑148 (MQ=255) cTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGt < 2:3320054/148‑1 (MQ=255) tGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGTAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAt < 2:2938851/148‑1 (MQ=255) gaaaatGCAACGTGATTCCGTGACGGCGAACAGCGCTCGTGTGGAGCACCATTTACAACTGTTGCAAGAAGCGGTAAACAGCACGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAtt < 1:1670868/146‑1 (MQ=255) gCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGACGCGGTAAACAGCAAGCGCCTGACTTTAACCTAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAtt < 1:2491054/148‑1 (MQ=255) gCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAGCAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTAtt > 2:2050021/1‑148 (MQ=255) aaaaaGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGTTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTc < 1:1085855/147‑1 (MQ=255) aGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGc > 2:1901766/1‑148 (MQ=255) aGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGc > 2:2493170/1‑148 (MQ=255) cAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTa < 1:279549/148‑1 (MQ=255) cAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTa < 2:1538595/148‑1 (MQ=255) cAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTa > 2:2338042/1‑148 (MQ=255) cAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTa > 2:1508192/1‑148 (MQ=255) tGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCg < 2:1394386/148‑1 (MQ=255) gATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGc > 2:1108749/1‑148 (MQ=255) aTTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCt > 1:3472395/1‑148 (MQ=255) ttACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTg > 2:199086/1‑148 (MQ=255) tACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTgg > 2:2421098/1‑148 (MQ=255) aCGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGt < 2:1369838/148‑1 (MQ=255) gTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGa < 2:149096/148‑1 (MQ=255) tGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGaa > 2:290305/1‑148 (MQ=255) gACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAg > 1:880339/1‑148 (MQ=255) cGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGa > 2:3309756/1‑148 (MQ=255) cGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGa < 1:1192436/148‑1 (MQ=255) cGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGa > 1:1002272/1‑148 (MQ=255) aaCAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAAc > 2:2674605/1‑148 (MQ=255) cAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTg > 2:2082647/1‑148 (MQ=255) cAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTg < 2:1530632/148‑1 (MQ=255) cgcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaa > 2:2048983/1‑148 (MQ=255) cGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTa > 1:1505062/1‑148 (MQ=255) cGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTa > 1:920067/1‑148 (MQ=255) cGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTa > 1:362460/1‑148 (MQ=255) cGTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTa > 2:1029272/1‑148 (MQ=255) cGTATGGAGAACCAGTTAAAACTGTTGAAAGAATGTTTAAACCCCAAGCGCCTGAATTTAACCTAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTa < 1:2109277/148‑1 (MQ=255) gTCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTaa < 1:1065710/148‑1 (MQ=255) tCTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAc < 2:2275201/148‑1 (MQ=255) cTGGAGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAAcc < 2:126317/148‑1 (MQ=255) aGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagca < 1:3508643/148‑1 (MQ=255) aGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagca < 1:286412/148‑1 (MQ=255) aGCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagca < 2:2197639/148‑1 (MQ=255) gCACCAGTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACcagcag > 2:36238/1‑148 (MQ=255) aGTTACAACTGTTGAAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAg < 1:455773/148‑1 (MQ=255) gTTACAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGt > 1:468464/1‑148 (MQ=255) aCAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAg < 1:2626764/148‑1 (MQ=255) aCAACTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAg < 2:1176649/148‑1 (MQ=255) aCTGTTGCAAGAATCGTTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGt < 1:1251332/148‑1 (MQ=255) cTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTc < 2:3403196/148‑1 (MQ=255) cTGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTc < 2:398085/148‑1 (MQ=255) tGTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCt > 1:2050253/1‑148 (MQ=255) gTTGCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTg > 1:3475716/1‑148 (MQ=255) gCAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGAtt > 1:1204141/1‑148 (MQ=255) cAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTa < 2:3114036/148‑1 (MQ=255) cAAGAATCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTa < 2:1344431/148‑1 (MQ=255) aagaatCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTAc < 1:664326/148‑1 (MQ=255) | AGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGACTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACC > CP009273/482614‑482900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |