Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 7,522 G→T 100% F146L (TTC→TTA)  yaaJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092737,5220GT99.0% 633.1 / ‑1.8 193F146L (TTC→TTA) yaaJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (99/92);  total (100/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

GAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACG  >  CP009273/7377‑7664
                                                                                                                                                 |                                                                                                                                              
 aGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAAc                                                                                                                                             <  1:1224184/148‑1 (MQ=255)
   tGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAg                                                                                                                                           >  2:2814006/1‑148 (MQ=255)
    gATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGa                                                                                                                                          <  1:3130966/148‑1 (MQ=255)
    gATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGa                                                                                                                                          <  1:2167080/148‑1 (MQ=255)
        gCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATCAGCAAAAAGACGGCTAACAGAACGc                                                                                                                                      <  2:3359597/148‑1 (MQ=255)
            gcagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCAt                                                                                                                                  <  1:2765769/148‑1 (MQ=255)
             cagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATc                                                                                                                                 >  1:906012/1‑148 (MQ=255)
             cagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATc                                                                                                                                 >  1:3028918/1‑148 (MQ=255)
             cagAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATc                                                                                                                                 >  1:2722298/1‑148 (MQ=255)
              agagTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATcc                                                                                                                                >  2:2015188/1‑148 (MQ=255)
                   aaaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAgcgc                                                                                                                           <  2:61035/148‑1 (MQ=255)
                    aaGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCa                                                                                                                          >  1:2315593/1‑148 (MQ=255)
                      gACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATc                                                                                                                        >  1:1042855/1‑148 (MQ=255)
                      gACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATc                                                                                                                        >  2:1793165/1‑148 (MQ=255)
                      gACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATc                                                                                                                        >  2:1264186/1‑148 (MQ=255)
                      gACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATc                                                                                                                        >  1:1232916/1‑148 (MQ=255)
                       aCAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATcc                                                                                                                       <  1:397160/148‑1 (MQ=255)
                           cGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAg                                                                                                                   >  2:211318/1‑148 (MQ=255)
                              taataatGCCTGTCACCAGCGGAGTAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGccc                                                                                                                <  1:2011183/147‑1 (MQ=255)
                              gaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGccc                                                                                                                >  2:1980509/1‑148 (MQ=255)
                              gaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGccc                                                                                                                >  2:3181414/1‑148 (MQ=255)
                              gaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGccc                                                                                                                >  2:3181563/1‑148 (MQ=255)
                              gaATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGccc                                                                                                                >  2:182795/1‑148 (MQ=255)
                                      ccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCa                                                                                                        >  2:2232061/1‑148 (MQ=255)
                                       cTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCat                                                                                                       <  2:250314/148‑1 (MQ=255)
                                        tGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCata                                                                                                      >  2:384626/1‑148 (MQ=255)
                                        tGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCata                                                                                                      >  2:2865237/1‑148 (MQ=255)
                                          tCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCAtata                                                                                                    >  1:2745655/1‑148 (MQ=255)
                                           cACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATAc                                                                                                   >  2:2848334/1‑148 (MQ=255)
                                             ccAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCa                                                                                                 >  1:2858739/1‑148 (MQ=255)
                                               aGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATg                                                                                               >  2:1091104/1‑148 (MQ=255)
                                                  gggggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGATTGAACTCCGCTTAAAATTATGCCATAGGCTATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCg                                                                                            <  1:776798/148‑1 (MQ=255)
                                                   ggggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCgg                                                                                           >  2:2360113/1‑148 (MQ=255)
                                                    gggAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGt                                                                                          >  1:1572089/1‑148 (MQ=255)
                                                          aTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCa                                                                                    >  2:3103426/1‑148 (MQ=255)
                                                          aTCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCa                                                                                    >  1:2078011/1‑148 (MQ=255)
                                                           tCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCAc                                                                                   >  1:1305707/1‑148 (MQ=255)
                                                           tCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCAc                                                                                   >  1:1346956/1‑148 (MQ=255)
                                                             aaaaGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGa                                                                                 >  2:1722979/1‑148 (MQ=255)
                                                                aGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAAc                                                                              <  1:2553883/148‑1 (MQ=255)
                                                                aGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCAGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAAc                                                                              <  2:1177770/148‑1 (MQ=255)
                                                                    aaaCTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGcc                                                                          <  1:408437/148‑1 (MQ=255)
                                                                       cTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCAt                                                                       <  1:1448241/148‑1 (MQ=255)
                                                                         cAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTg                                                                     <  2:1461165/148‑1 (MQ=255)
                                                                         cAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTg                                                                     >  2:1774914/1‑148 (MQ=255)
                                                                          aGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGa                                                                    <  1:317232/148‑1 (MQ=255)
                                                                          aGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGa                                                                    <  1:384626/148‑1 (MQ=255)
                                                                          aGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGa                                                                    <  2:1691876/148‑1 (MQ=255)
                                                                          aGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGa                                                                    <  1:2175017/148‑1 (MQ=255)
                                                                           gggCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGAc                                                                   >  2:2297224/1‑148 (MQ=255)
                                                                             gcgcGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGt                                                                 <  1:387211/148‑1 (MQ=255)
                                                                               gcgGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCa                                                               <  2:1042855/148‑1 (MQ=255)
                                                                                 gggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACg                                                             <  2:2650876/148‑1 (MQ=255)
                                                                                  ggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGt                                                            >  1:2378719/1‑148 (MQ=255)
                                                                                  ggCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGt                                                            <  2:3259522/148‑1 (MQ=255)
                                                                                   gCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGTCGATGAGCAAAAAGACGGCTAACAGAACGACCATCCAGCGCATCCCCAGCACGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGtt                                                           <  1:2976851/148‑1 (MQ=255)
                                                                                     aaCGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCt                                                         <  1:164664/148‑1 (MQ=255)
                                                                                       cGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCttt                                                       <  2:45670/148‑1 (MQ=255)
                                                                                       cGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCttt                                                       <  1:2130849/148‑1 (MQ=255)
                                                                                        ggCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTta                                                      >  1:1798917/1‑148 (MQ=255)
                                                                                            ttCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATaaa                                                  <  2:1258876/148‑1 (MQ=255)
                                                                                             tCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                 <  2:370172/148‑1 (MQ=255)
                                                                                             tCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                 >  1:169809/1‑148 (MQ=255)
                                                                                             tCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAg                                                 <  2:545073/148‑1 (MQ=255)
                                                                                              cGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGt                                                <  1:607480/148‑1 (MQ=255)
                                                                                              cGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGt                                                <  1:3380815/148‑1 (MQ=255)
                                                                                               gCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGtt                                               >  1:686573/1‑148 (MQ=255)
                                                                                                 ttGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTtgt                                             >  2:93633/1‑148 (MQ=255)
                                                                                                   gAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGc                                           >  2:3466454/1‑148 (MQ=255)
                                                                                                   gAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGc                                           <  1:2177341/148‑1 (MQ=255)
                                                                                                   gAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGc                                           <  1:3507719/148‑1 (MQ=255)
                                                                                                       tCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCaga                                       >  1:723095/1‑148 (MQ=255)
                                                                                                         cGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAgaga                                     <  1:2885898/148‑1 (MQ=255)
                                                                                                         cGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAgaga                                     <  1:1026579/148‑1 (MQ=255)
                                                                                                         cGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAgaga                                     <  2:396492/148‑1 (MQ=255)
                                                                                                           cTGACAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATAGACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATTAAGTTGTGCCAGAGAAc                                   <  2:2921885/148‑1 (MQ=255)
                                                                                                             gAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAt                                 >  1:3302078/1‑148 (MQ=255)
                                                                                                              aaaaTTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACAtt                                <  2:1295578/148‑1 (MQ=255)
                                                                                                               aaaTTATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTc                               <  2:586650/148‑1 (MQ=255)
                                                                                                                  ttATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATNTACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGc                            >  1:1803732/1‑148 (MQ=255)
                                                                                                                  ttATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGc                            >  1:1470951/1‑148 (MQ=255)
                                                                                                                   tATGCCATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCa                           >  1:3311722/1‑148 (MQ=255)
                                                                                                                       ccATAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACg                       >  2:1942935/1‑148 (MQ=255)
                                                                                                                         aTAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAg                     <  1:3518009/148‑1 (MQ=255)
                                                                                                                         aTAGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAg                     <  1:1761029/148‑1 (MQ=255)
                                                                                                                           aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGACCATTCGGCAAACGAGGt                   >  2:3517109/1‑148 (MQ=255)
                                                                                                                           aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGt                   <  1:2814006/148‑1 (MQ=255)
                                                                                                                           aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGt                   <  1:457218/148‑1 (MQ=255)
                                                                                                                           aGGCGATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGCCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGt                   >  2:2719865/1‑148 (MQ=255)
                                                                                                                               gATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGcc               <  1:1115731/148‑1 (MQ=255)
                                                                                                                               gATGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGcc               <  1:416493/148‑1 (MQ=255)
                                                                                                                                aTGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCa              <  1:969348/148‑1 (MQ=255)
                                                                                                                                 tGAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCAt             >  2:2469762/1‑148 (MQ=255)
                                                                                                                                  gAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATg            >  1:1958991/1‑148 (MQ=255)
                                                                                                                                  gAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATg            >  2:2330908/1‑148 (MQ=255)
                                                                                                                                  gAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATg            >  2:2960611/1‑148 (MQ=255)
                                                                                                                                  gAGCAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATg            <  2:863945/148‑1 (MQ=255)
                                                                                                                                     cAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCg         <  2:2741306/148‑1 (MQ=255)
                                                                                                                                       aaaaGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGAt       >  1:1099953/1‑148 (MQ=255)
                                                                                                                                            aCGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAcg  <  1:2269017/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACG  >  CP009273/7377‑7664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑