Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 500,234 C→A 9.8% intergenic (‑82/‑136) fsr ← / → ushA putative fosmidomycin efflux system protein/bifunctional UDP‑sugar hydrolase/5'‑nucleotidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273500,2340CA 9.8% 717.8 / 35.4 204intergenic (‑82/‑136)fsr/ushAputative fosmidomycin efflux system protein/bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base C (97/87);  new base A (8/12);  total (105/99)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 219 total aligned reads displayed.

TGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTG  >  CP009273/500087‑500381
                                                                                                                                                   |                                                                                                                                                   
 gTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgtcg                                                                                                                                                    <  2:846121/148‑1 (MQ=255)
  ttCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgtcgt                                                                                                                                                   >  2:2282856/1‑148 (MQ=255)
  ttCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgtcgt                                                                                                                                                   >  1:139290/1‑148 (MQ=255)
  ttCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGcgtcgt                                                                                                                                                   >  2:492157/1‑148 (MQ=255)
    cGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCt                                                                                                                                                 <  1:529000/148‑1 (MQ=255)
      ggCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTga                                                                                                                                               >  1:403074/1‑148 (MQ=255)
       gCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTgaa                                                                                                                                              <  2:292505/148‑1 (MQ=255)
       gCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTgaa                                                                                                                                              <  1:13815/148‑1 (MQ=255)
               cGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGtct                                                                                                                                      <  1:3202824/148‑1 (MQ=255)
                 ttGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCa                                                                                                                                    <  1:2167717/148‑1 (MQ=255)
                  tGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCaa                                                                                                                                   >  2:358978/1‑148 (MQ=255)
                    aCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACg                                                                                                                                 >  2:1139671/1‑148 (MQ=255)
                    aCGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACg                                                                                                                                 >  2:444300/1‑148 (MQ=255)
                       cAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCg                                                                                                                              <  1:2706040/148‑1 (MQ=255)
                         gCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGaa                                                                                                                            <  1:1333651/148‑1 (MQ=255)
                            gcgcCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATAc                                                                                                                         <  2:2806381/148‑1 (MQ=255)
                                ccgccACAGGCTGGGGTTGTTCACTCATTGACATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGaa                                                                                                                     <  2:1311290/148‑1 (MQ=255)
                                 cgccACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAAt                                                                                                                    <  1:287770/148‑1 (MQ=255)
                                     acaGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCt                                                                                                                <  2:2952682/148‑1 (MQ=255)
                                        ggCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAAt                                                                                                             >  2:1697891/1‑148 (MQ=255)
                                         gCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATATGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGACGGACGTCTCAACGCCGAATACAGAATTTCTAATc                                                                                                            >  2:2859150/1‑148 (MQ=255)
                                            ggggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTgg                                                                                                         >  1:1778441/1‑148 (MQ=255)
                                            ggggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTgg                                                                                                         >  1:2569192/1‑148 (MQ=255)
                                              ggTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGAt                                                                                                       >  2:1679492/1‑148 (MQ=255)
                                               gttgttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATg                                                                                                      >  1:632275/1‑148 (MQ=255)
                                                ttgttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGc                                                                                                     >  2:1451171/1‑148 (MQ=255)
                                                ttgttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGc                                                                                                     <  1:269120/148‑1 (MQ=255)
                                                   ttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGa                                                                                                  <  1:2465305/148‑1 (MQ=255)
                                                   ttCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGa                                                                                                  <  2:2083422/148‑1 (MQ=255)
                                                    tCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGAt                                                                                                 <  1:3292498/148‑1 (MQ=255)
                                                      aCTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGAttt                                                                                               >  1:3171358/1‑148 (MQ=255)
                                                       cTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTa                                                                                              >  2:369243/1‑148 (MQ=255)
                                                       cTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTa                                                                                              >  1:1436808/1‑148 (MQ=255)
                                                        tCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTAt                                                                                             <  1:267347/148‑1 (MQ=255)
                                                            tGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTc                                                                                         <  1:2063220/148‑1 (MQ=255)
                                                            tGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTc                                                                                         >  1:2370697/1‑148 (MQ=255)
                                                             gCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCa                                                                                        <  2:1906636/148‑1 (MQ=255)
                                                             gCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCa                                                                                        <  2:2741807/148‑1 (MQ=255)
                                                             gCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTAGATGCAGATTTATCTTCa                                                                                        <  2:3385004/148‑1 (MQ=255)
                                                                 tAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCgg                                                                                    >  1:2289592/1‑148 (MQ=255)
                                                                 tAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCgg                                                                                    >  1:1238463/1‑148 (MQ=255)
                                                                 tAGGAAAGTCACATTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCgg                                                                                    >  1:2050712/1‑148 (MQ=255)
                                                                  aGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGa                                                                                   >  2:2563056/1‑148 (MQ=255)
                                                                     aaaGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGc                                                                                >  2:20115/1‑148 (MQ=255)
                                                                       aGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAg                                                                              >  2:534564/1‑148 (MQ=255)
                                                                          cACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACt                                                                           <  1:869919/148‑1 (MQ=255)
                                                                            cTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTg                                                                         >  1:3084309/1‑148 (MQ=255)
                                                                             tttttCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGt                                                                        >  2:2641222/1‑148 (MQ=255)
                                                                                ttCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGGCGCAGACTTGTCTa                                                                     <  2:2318580/148‑1 (MQ=255)
                                                                                  cAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatg                                                                   <  2:3427619/148‑1 (MQ=255)
                                                                                   aGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatga                                                                  <  2:3522716/148‑1 (MQ=255)
                                                                                    gggTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatgat                                                                 <  1:2235388/148‑1 (MQ=255)
                                                                                    gggTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACAGACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatgat                                                                 <  1:447469/148‑1 (MQ=255)
                                                                                     ggTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTatgatg                                                                >  2:206968/1‑148 (MQ=255)
                                                                                       ttGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTc                                                              >  2:728299/1‑148 (MQ=255)
                                                                                        tGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTcg                                                             >  2:2038984/1‑148 (MQ=255)
                                                                                            aTGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGTTGTCGCGTc                                                         >  2:1959677/1‑148 (MQ=255)
                                                                                              gTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCAt                                                       <  1:1139671/148‑1 (MQ=255)
                                                                                              gTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCAt                                                       <  2:1509901/148‑1 (MQ=255)
                                                                                               tAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATa                                                      >  2:2735505/1‑148 (MQ=255)
                                                                                                aaaGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATAc                                                     >  2:2265800/1‑148 (MQ=255)
                                                                                                aaaGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATAc                                                     >  2:2774037/1‑148 (MQ=255)
                                                                                                aaaGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATAc                                                     >  2:1044423/1‑148 (MQ=255)
                                                                                                 aaGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACt                                                    >  2:807601/1‑148 (MQ=255)
                                                                                                 aaGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACt                                                    <  2:403074/148‑1 (MQ=255)
                                                                                                   gAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTAt                                                  >  1:1033632/1‑148 (MQ=255)
                                                                                                    aaTGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTAtt                                                 >  1:339249/1‑148 (MQ=255)
                                                                                                    aaTGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTAtt                                                 >  1:149154/1‑148 (MQ=255)
                                                                                                     aTGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTAttt                                                <  2:2795354/148‑1 (MQ=255)
                                                                                                          cTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCaa                                           <  2:1064889/148‑1 (MQ=255)
                                                                                                            tATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAaca                                         <  1:3447495/148‑1 (MQ=255)
                                                                                                            tATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAaca                                         >  2:1603209/1‑148 (MQ=255)
                                                                                                            tATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAaca                                         <  1:1283582/148‑1 (MQ=255)
                                                                                                               ttGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGt                                      <  1:3483364/148‑1 (MQ=255)
                                                                                                                tgtgATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGtt                                     >  1:831117/1‑148 (MQ=255)
                                                                                                                   gATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGaa                                  <  1:1012209/148‑1 (MQ=255)
                                                                                                                   gATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGaa                                  <  2:1249553/148‑1 (MQ=255)
                                                                                                                     ttattaCCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAt                                <  1:2774037/148‑1 (MQ=255)
                                                                                                                     ttattaCCAGACTAACATACCTGTATGCGTAGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAt                                >  2:1555757/1‑148 (MQ=255)
                                                                                                                      tattaCCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtc                               <  2:623385/148‑1 (MQ=255)
                                                                                                                         taCCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcagg                            >  2:2208939/1‑148 (MQ=255)
                                                                                                                         taCCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcagg                            <  2:3461514/148‑1 (MQ=255)
                                                                                                                         taCCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcagg                            >  2:3437364/1‑148 (MQ=255)
                                                                                                                           ccAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcaggtc                          >  1:1336563/1‑148 (MQ=255)
                                                                                                                           ccAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcaggtc                          >  2:191324/1‑148 (MQ=255)
                                                                                                                             aGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAAtcaggtcag                        <  1:2990382/148‑1 (MQ=255)
                                                                                                                                   aCATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAATTCAGGTCAGGgagag                  >  1:1235363/1‑148 (MQ=255)
                                                                                                                                       aCCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGt              <  1:2013010/148‑1 (MQ=255)
                                                                                                                                       aCCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGt              >  1:1225179/1‑148 (MQ=255)
                                                                                                                                          tGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATg           <  2:3171358/148‑1 (MQ=255)
                                                                                                                                          tGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATg           >  1:777851/1‑148 (MQ=255)
                                                                                                                                          tGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATg           <  1:158550/148‑1 (MQ=255)
                                                                                                                                           gTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGa          >  1:2361405/1‑148 (MQ=255)
                                                                                                                                            tATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGaa         <  1:1982914/148‑1 (MQ=255)
                                                                                                                                             aTGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGaaa        >  2:3088829/1‑148 (MQ=255)
                                                                                                                                             aTGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGaaa        <  2:3004087/148‑1 (MQ=255)
                                                                                                                                              tGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAat       >  2:184194/1‑148 (MQ=255)
                                                                                                                                                cgtcgtCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAatta     <  2:1041712/148‑1 (MQ=255)
                                                                                                                                                   cgtcTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTg  <  1:1697891/148‑1 (MQ=255)
                                                                                                                                                   cgtcTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTg  <  1:231617/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGTTCGGGCCTTGGTCGTTGACGCAGCCGCGCCCGCCACAGGCTGGGGTTGTTCACTCATTGCCATAGGAAAGTCACTTTTTCAGGGTTGCGATGTAAAGAATGATCTTATTTGTGATTATTACCAGACTAACATACCTGTATGCGTCGTCTGAAGGAAGTCTCAACGCCGAATACAGAATTTCTAATCTGGATGCAGATTTATCTTCACCGGACGCAGACTTGTCTATGATGTCGCGTCATACTATTTTTCAACACGTTGAAATCAGGTCAGGGAGAGAAGTATGAAATTATTG  >  CP009273/500087‑500381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑