Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 503,944 G→T 100% E91* (GAA→TAA)  ybaQ → putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273503,9440GT98.7% 542.4 / ‑6.2 191E91* (GAA→TAA) ybaQputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (3/1);  major base T (84/92);  minor base A (11/0);  total (98/93)
Fisher's exact test for biased strand distribution p-value = 7.26e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCC  >  CP009273/503799‑504088
                                                                                                                                                  |                                                                                                                                                
tGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGGTGATGTTTGGGGAGTTTTAAACAACATGGG‑CCCCCCGgcagaa                                                                                                                                                >  1:3147164/1‑148 (MQ=255)
 gCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGaataat                                                                                                                                               <  2:2124388/148‑1 (MQ=255)
   aCTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTg                                                                                                                                             <  1:522720/148‑1 (MQ=255)
     tGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTggg                                                                                                                                           <  2:3340163/148‑1 (MQ=255)
      gATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCTAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAACCTCCACGCGGCGGTTGATGTTTTGGGAGTTTTAAAAAACAAGCG‑CACCCAGgaataaattggt                                                                                                                                          >  1:1039688/1‑142 (MQ=255)
        tCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGCTCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAAAAATTTGGACg                                                                                                                                        >  2:1607006/1‑148 (MQ=255)
         cAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACgg                                                                                                                                       >  2:2606546/1‑148 (MQ=255)
           aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAACCAACAGGCGGCGGTTTGTGTTTGGGAAGTTGTAAACAACATTCG‑CACCCCCGCATAAATGGGACggga                                                                                                                                     >  1:2767189/1‑146 (MQ=255)
           aTAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGAt                                                                                                                                     <  1:1531674/148‑1 (MQ=255)
             aaCAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTg                                                                                                                                   >  2:1406514/1‑148 (MQ=255)
               cAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCACGCGGCGGTTGTTATGTTGGTAGTTGAAAACAACATGCG‑CACCCACGAATAATTGGGCAGGAtttta                                                                                                                                 >  1:2529163/1‑145 (MQ=255)
                 aTCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAACCAACAGGCGGCGGTTGATCTTTGGGAAGTTGTAAAAAACAATCG‑CACCCAGGAATAAATTGGAGGGAttttaaa                                                                                                                               >  1:1885294/1‑143 (MQ=255)
                    gTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGAGTTTTTGCTAAACCAACAGGCGGCGGGTGTTGTTTGGGTAGTTTTAAAAAACAAGCG‑CACCCCGGGAAAATTTGGGAGGTTTGTAACact                                                                                                                            >  1:1019509/1‑146 (MQ=255)
                      aaaCTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGGTGATCGTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTgg                                                                                                                          >  2:3527167/1‑148 (MQ=255)
                             ctactGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAata                                                                                                                   <  2:2711812/148‑1 (MQ=255)
                               actGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATAtt                                                                                                                 <  2:2556059/148‑1 (MQ=255)
                                ctGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCACCAGGCGGCGGTTGATCTTTGGGAAGGTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATAttt                                                                                                                >  2:1651543/1‑148 (MQ=255)
                                     aTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAACCTCCAGGCGGGGGGTGCTCTTTTGGTAGTTGTAAACAAAATGCG‑CACCCAGGGATAATTGGGTCGGGGTGTAAAAGTGGCGGAATATTTGGCAc                                                                                                           >  2:2683195/1‑148 (MQ=255)
                                     aTGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAAACTCCAGGCGGCGGTTGATGTTTGGGAAGTTTTAAAAAACAAGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAAAATTTTGCAc                                                                                                           >  2:745353/1‑148 (MQ=255)
                                       ggCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTTGGTAAACCAACAGGCGGCGGGTGGTCTTTTGGAAGTTTAAAAAAAAAAGCGCCCCCCCGCCAAAATTGGG‑CGGGTTTAAACACTGGCTGAATATTTTGCAccc                                                                                                         >  1:1384611/1‑146 (MQ=255)
                                         cATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCg                                                                                                       <  2:1914515/148‑1 (MQ=255)
                                           tttCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAAACTCCAGGCGGGGGGTTGTCTTTTGGAAGTTGTAAAAAACAATCG‑CACCCCGGACTAATTGGGCCGGGTTTGAACACTGGGTGAATATTTTGCACGCCGTg                                                                                                     >  1:830921/1‑148 (MQ=255)
                                            ttCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGa                                                                                                    <  1:2800618/148‑1 (MQ=255)
                                                tCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGGTAAAACTCCAGGCGGCGGTTGTTCTTTTGGAAGTTGAAAAAAAAATGCG‑CACCCCGGGAAAATTGGGACGGTTTGGAACAGTGGCTGTATATTTGGCACGCCGGGTagag                                                                                                >  2:1343115/1‑148 (MQ=255)
                                                  tGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCg                                                                                              <  2:2086590/148‑1 (MQ=255)
                                                    gCGAAAGTTTTTGATACCACAGTCGATTTTTTGCTAAACCTCCAGGCGGCGGTTGTTATTTTGGAAGTTTAAAAAAAAATGCG‑CACCCAGGGATAAATGGGTCGGATTTTAAAACTGGCTGTATATTTTGCAAGCCGTGAAGAGCGTg                                                                                            >  1:2955260/1‑148 (MQ=255)
                                                       aaaGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaa                                                                                         >  2:2373386/1‑148 (MQ=255)
                                                        aaGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaaa                                                                                        <  1:1772242/148‑1 (MQ=255)
                                                          gTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCaaaaa                                                                                      <  2:962498/148‑1 (MQ=255)
                                                            ttttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAg                                                                                    <  1:2557031/148‑1 (MQ=255)
                                                            ttttGATACCACAGTCGATTTTTGGCTAAAACACCACGCGGCGGTTGATATTTGGGAAATTGAAAACAAAAAGCG‑CACCCAGGAATAATTTGGGCGGATTGTAAAAGTGGCTGAATATTTTGCACGCCGGGAAGAGCGTGCAAAAAAg                                                                                    >  1:3516102/1‑148 (MQ=255)
                                                             tttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTTAAACCGTGGCTGAATATTTGGCTCGCCGTGAAGAGCGTGCACAAAAgg                                                                                   >  2:3455797/1‑148 (MQ=255)
                                                             tttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAgg                                                                                   <  1:3084510/148‑1 (MQ=255)
                                                             tttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAgg                                                                                   <  2:1549387/148‑1 (MQ=255)
                                                              ttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGt                                                                                  <  2:401827/148‑1 (MQ=255)
                                                              ttGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGt                                                                                  <  1:864775/148‑1 (MQ=255)
                                                               tGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTc                                                                                 <  1:1995460/148‑1 (MQ=255)
                                                                gATACCACAGTCGATTTTTTGCTAAAACTCCAGGCGGCGGTTGATCTTTGGGAAGTTGTAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGTAGAGCGTGCAAAAAAGGTcg                                                                                >  2:2512260/1‑148 (MQ=255)
                                                                gATACCACAGTCGATTTTTGGGTAAAACTCCAGGCGGGGGTTTATCTTTGGGCAGTTGTAAACAACAAGCG‑CACCCAGGAATAATTGGGGCGGATTGAAACACTGGCTGTATATTTTGCACGCCGTGAAGAGCGTGCAAAAAAGGTcg                                                                                >  2:729795/1‑148 (MQ=255)
                                                                 aTACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCGTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTcgc                                                                               >  2:2849458/1‑148 (MQ=255)
                                                                     cacaGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTaa                                                                           <  1:267380/148‑1 (MQ=255)
                                                                      acaGTCGATTTTTGGCTAAAACACCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTTAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAg                                                                          >  2:2256628/1‑148 (MQ=255)
                                                                           cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaa                                                                     >  2:1751956/1‑148 (MQ=255)
                                                                           cGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaa                                                                     >  2:2303438/1‑148 (MQ=255)
                                                                            gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaa                                                                    >  2:3270151/1‑148 (MQ=255)
                                                                            gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaa                                                                    >  2:51292/1‑148 (MQ=255)
                                                                            gATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaa                                                                    >  2:1504689/1‑148 (MQ=255)
                                                                             aTTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCaaaa                                                                   <  2:623126/148‑1 (MQ=255)
                                                                              tttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                  >  1:1693131/1‑148 (MQ=255)
                                                                              tttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAc                                                                  >  1:3120246/1‑148 (MQ=255)
                                                                               ttttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACa                                                                 >  1:182772/1‑148 (MQ=255)
                                                                                tttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACAt                                                                <  1:1255297/148‑1 (MQ=255)
                                                                                tttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACAt                                                                <  2:1193212/148‑1 (MQ=255)
                                                                                tttGGATAAACCTCGATGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACTGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACAt                                                                <  2:2533441/148‑1 (MQ=255)
                                                                                 ttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACCTGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGGGGCGTAACGGCaaaaaaac                                                               >  1:2125625/1‑144 (MQ=255)
                                                                                 ttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATc                                                               >  1:335983/1‑148 (MQ=255)
                                                                                 ttGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATc                                                               >  1:333808/1‑148 (MQ=255)
                                                                                  tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCg                                                              <  2:1964075/148‑1 (MQ=255)
                                                                                  tGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCg                                                              <  1:1017090/148‑1 (MQ=255)
                                                                                    gCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCt                                                            <  1:585193/148‑1 (MQ=255)
                                                                                     cTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCtt                                                           >  1:2450851/1‑148 (MQ=255)
                                                                                      tAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTc                                                          >  1:3499863/1‑148 (MQ=255)
                                                                                       aaaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGTATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCa                                                         <  2:2137344/148‑1 (MQ=255)
                                                                                       aaaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCa                                                         <  2:2831370/148‑1 (MQ=255)
                                                                                        aaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAt                                                        <  1:3301277/148‑1 (MQ=255)
                                                                                        aaCCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCAt                                                        <  2:2530420/148‑1 (MQ=255)
                                                                                             ccAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGt                                                   <  2:2955260/148‑1 (MQ=255)
                                                                                              cAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTa                                                  <  1:2201106/148‑1 (MQ=255)
                                                                                                ggcggcGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAgg                                                >  2:2487097/1‑148 (MQ=255)
                                                                                                ggcggcGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAgg                                                <  2:2635425/148‑1 (MQ=255)
                                                                                                 gcggcgGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGGACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                               >  2:655250/1‑148 (MQ=255)
                                                                                                 gcggcgGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGc                                               >  2:2842720/1‑148 (MQ=255)
                                                                                                    gcggTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCgg                                            <  1:1194693/148‑1 (MQ=255)
                                                                                                      ggTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGAt                                          <  2:1218826/148‑1 (MQ=255)
                                                                                                       gTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATa                                         <  2:239511/148‑1 (MQ=255)
                                                                                                       gTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATa                                         <  2:2203009/148‑1 (MQ=255)
                                                                                                        ttGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATaa                                        >  2:705666/1‑148 (MQ=255)
                                                                                                         tGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAg                                       <  1:1242908/148‑1 (MQ=255)
                                                                                                            tCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCg                                    <  1:2735255/148‑1 (MQ=255)
                                                                                                               ttGGGAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTc                                 <  2:3555439/148‑1 (MQ=255)
                                                                                                                  ggAAGTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcg                              >  1:2640125/1‑148 (MQ=255)
                                                                                                                      gTTGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCAcgccgc                          <  2:1864484/148‑1 (MQ=255)
                                                                                                                       ttGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgca                         >  1:921154/1‑148 (MQ=255)
                                                                                                                        tGAAAACAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcat                        <  1:3527185/148‑1 (MQ=255)
                                                                                                                          aaaaCAACATGCG‑CACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAAAATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatcc                      >  2:2896283/1‑148 (MQ=255)
                                                                                                                           aaaCAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccg                     >  1:2859629/1‑148 (MQ=255)
                                                                                                                           aaaCAACATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccg                     >  2:2993902/1‑148 (MQ=255)
                                                                                                                            aacaacATGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccgg                    <  1:322016/148‑1 (MQ=255)
                                                                                                                              caacaTGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggca                  >  1:4156/1‑148 (MQ=255)
                                                                                                                               aacaTGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcat                 >  1:59107/1‑148 (MQ=255)
                                                                                                                                 caTGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatcc               >  2:3377604/1‑148 (MQ=255)
                                                                                                                                  aTGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatccg              <  1:2373386/148‑1 (MQ=255)
                                                                                                                                  aTGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCgcatccggcatccg              >  2:2039329/1‑148 (MQ=255)
                                                                                                                                   tGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGa             >  1:1487066/1‑148 (MQ=255)
                                                                                                                                   tGCG‑CACCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGa             >  1:814386/1‑148 (MQ=255)
                                                                                                                                         aCCCAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCAcc        >  1:1636409/1‑148 (MQ=255)
                                                                                                                                          cccAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCa       <  2:1267689/148‑1 (MQ=255)
                                                                                                                                            cAGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCAtt     >  1:2312657/1‑148 (MQ=255)
                                                                                                                                             aGGAATAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTg    >  1:3032410/1‑148 (MQ=255)
                                                                                                                                               gaataaTTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGcc  >  1:151604/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TGCACTGATCAATAACAATCGTAAACTCACTACTGAGATGGCATTTCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCGGTTGATCTTTGGGAAGTTGAAAACAACATGCG‑CACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGAGCGTGCAAAAAAGGTCGCGTAAGGCCAAAACATCGCTTCATCTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCC  >  CP009273/503799‑504088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑