Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 551,602 A→T 29.4% F136L (TTT→TTA)  ybcI ← DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273551,6020AT29.4% 289.3 / 159.0 180F136L (TTT→TTA) ybcIDUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (64/63);  new base T (29/24);  total (93/87)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

CGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGC  >  CP009273/551456‑551747
                                                                                                                                                  |                                                                                                                                                 
cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCaa                                                                                                                                                  <  1:3043021/148‑1 (MQ=255)
  cTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAAc                                                                                                                                                >  2:1300474/1‑148 (MQ=255)
   tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACg                                                                                                                                               <  1:2188961/148‑1 (MQ=255)
    aTTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACgg                                                                                                                                              <  2:471118/148‑1 (MQ=255)
     ttGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGgc                                                                                                                                             >  1:2008040/1‑148 (MQ=255)
     ttGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGgc                                                                                                                                             >  2:1526650/1‑148 (MQ=255)
         cGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa                                                                                                                                         <  2:993123/148‑1 (MQ=255)
          ggATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGAc                                                                                                                                        <  2:1049997/148‑1 (MQ=255)
            aTGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtt                                                                                                                                      <  2:539782/148‑1 (MQ=255)
             tGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACttt                                                                                                                                     <  2:1372317/148‑1 (MQ=255)
             tGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACttt                                                                                                                                     <  2:3141523/148‑1 (MQ=255)
               cGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTa                                                                                                                                   <  2:2616034/148‑1 (MQ=255)
                   ttttCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAc                                                                                                                               <  1:3182215/148‑1 (MQ=255)
                    tttCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCAcc                                                                                                                              <  1:18240/148‑1 (MQ=255)
                     ttCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCt                                                                                                                             >  2:215610/1‑148 (MQ=255)
                      tCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTg                                                                                                                            >  2:2813524/1‑148 (MQ=255)
                       cGCCTCCGGTTATCGTCTGCTCCACCACCCCCTTCCCATCAGTAGCATCCTCGGCACCCACCCCCACATCAATTCACAAATAAACACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGc                                                                                                                           <  2:1201693/148‑1 (MQ=255)
                       cGCATCCGGTTATCGTCTGCTCCACCCCAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTATTATCACCTGc                                                                                                                           <  2:3211735/148‑1 (MQ=255)
                       cGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGc                                                                                                                           <  2:1255783/148‑1 (MQ=255)
                       cGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGc                                                                                                                           <  2:1255779/148‑1 (MQ=255)
                          aTCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAg                                                                                                                        <  2:1002377/148‑1 (MQ=255)
                            ccGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAggg                                                                                                                      >  2:2741172/1‑148 (MQ=255)
                              ggTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAg                                                                                                                    <  1:3506610/148‑1 (MQ=255)
                              ggTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAg                                                                                                                    <  1:1747928/148‑1 (MQ=255)
                              ggTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAg                                                                                                                    <  1:284582/148‑1 (MQ=255)
                              ggTTATCGTCTGCTCCACCACAACATGCCCAACAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAg                                                                                                                    <  1:2113899/148‑1 (MQ=255)
                               gTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGc                                                                                                                   >  1:1443309/1‑148 (MQ=255)
                                ttATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCg                                                                                                                  >  1:1020237/1‑148 (MQ=255)
                                 tATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGa                                                                                                                 >  2:3008336/1‑148 (MQ=255)
                                   tCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaa                                                                                                               >  1:1612476/1‑148 (MQ=255)
                                    cGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGaaaa                                                                                                              >  2:1895195/1‑148 (MQ=255)
                                      tCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAg                                                                                                            >  1:628850/1‑148 (MQ=255)
                                      tCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAg                                                                                                            >  1:2819678/1‑148 (MQ=255)
                                      tCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAg                                                                                                            >  1:1044371/1‑148 (MQ=255)
                                        tGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCg                                                                                                          <  2:1816100/148‑1 (MQ=255)
                                             caccacAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGAATTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCAt                                                                                                     <  1:1335562/148‑1 (MQ=255)
                                               ccacAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCt                                                                                                   >  1:3119238/1‑148 (MQ=255)
                                                   aacaTTCCCATCAGGAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGAcc                                                                                               <  2:3010645/148‑1 (MQ=255)
                                                    acaTTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCa                                                                                              >  2:2793679/1‑148 (MQ=255)
                                                     caTTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCAc                                                                                             >  2:2985922/1‑148 (MQ=255)
                                                        tCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGc                                                                                          >  2:2930308/1‑148 (MQ=255)
                                                        tCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGc                                                                                          >  1:548383/1‑148 (MQ=255)
                                                        tCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGc                                                                                          <  2:2955033/148‑1 (MQ=255)
                                                         cccATCAGTAGCCTCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCGGATGCCCTTACGGCGGGGTGTAACGAGACAATGCACACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGcc                                                                                         <  1:904324/148‑1 (MQ=255)
                                                         cccATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGcc                                                                                         >  2:1105060/1‑148 (MQ=255)
                                                          ccATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCca                                                                                        <  2:2931589/148‑1 (MQ=255)
                                                             tCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCGGATGCCCGTAGGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcag                                                                                     <  1:229253/148‑1 (MQ=255)
                                                                gTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagcca                                                                                  >  1:2535191/1‑148 (MQ=255)
                                                                 tAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCAcagccag                                                                                 >  1:192751/1‑148 (MQ=255)
                                                                   gCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACagccagcc                                                                               >  1:2853505/1‑148 (MQ=255)
                                                                   gCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACagccagcc                                                                               >  1:1767445/1‑148 (MQ=255)
                                                                    cATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAgccagcca                                                                              >  1:696760/1‑148 (MQ=255)
                                                                    cATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAgccagcca                                                                              >  1:2318438/1‑148 (MQ=255)
                                                                    cATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAgccagcca                                                                              >  1:2732089/1‑148 (MQ=255)
                                                                    cATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAgccagcca                                                                              >  1:530408/1‑148 (MQ=255)
                                                                     aTCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCaa                                                                             <  2:1601021/148‑1 (MQ=255)
                                                                      tCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAAc                                                                            >  2:1935156/1‑148 (MQ=255)
                                                                         ccGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGcc                                                                         <  1:2647158/148‑1 (MQ=255)
                                                                             cAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTa                                                                     >  2:1177198/1‑148 (MQ=255)
                                                                             cAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTGTa                                                                     >  2:349839/1‑148 (MQ=255)
                                                                               aCCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTAcc                                                                   >  2:3095379/1‑148 (MQ=255)
                                                                                 cacaCCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGc                                                                 >  2:3258525/1‑148 (MQ=255)
                                                                                 cacaCCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGc                                                                 <  2:1601075/148‑1 (MQ=255)
                                                                                     cccACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACTAGACAATGCAAACGGCTCGACTTTTATCACCTGCCAGGGAGAGAAAAAGAGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGt                                                             >  1:1572777/1‑148 (MQ=255)
                                                                                         caTCAATGCAGAACTAATCACCTGAGGCCCGTACGGCGTGGTTTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGtt                                                         <  1:3458701/148‑1 (MQ=255)
                                                                                          aTCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGcc                                                                         <  1:3111649/131‑1 (MQ=255)
                                                                                           tCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTAc                                                       >  1:2904524/1‑148 (MQ=255)
                                                                                             aaTTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCg                                                     >  2:1855740/1‑148 (MQ=255)
                                                                                             aaTTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCg                                                     >  2:771568/1‑148 (MQ=255)
                                                                                               ttCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGaa                                                   >  2:2877037/1‑148 (MQ=255)
                                                                                                tCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAAt                                                  >  2:344470/1‑148 (MQ=255)
                                                                                                tCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAAt                                                  <  1:2571993/148‑1 (MQ=255)
                                                                                                tCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAAt                                                  <  1:911912/148‑1 (MQ=255)
                                                                                                 cAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATc                                                 <  2:495435/148‑1 (MQ=255)
                                                                                                   gAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCa                                               >  1:2524451/1‑148 (MQ=255)
                                                                                                   gAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCa                                               >  2:350303/1‑148 (MQ=255)
                                                                                                    aaaTAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAg                                              >  2:2678952/1‑148 (MQ=255)
                                                                                                      ataatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCa                                            <  1:3055381/148‑1 (MQ=255)
                                                                                                      ataatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCa                                            >  1:1664087/1‑148 (MQ=255)
                                                                                                       taatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCaa                                           >  1:1109073/1‑148 (MQ=255)
                                                                                                        aatCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAg                                          >  1:1396929/1‑148 (MQ=255)
                                                                                                         atCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGc                                         <  1:1300474/148‑1 (MQ=255)
                                                                                                          tCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCt                                        >  1:2908575/1‑148 (MQ=255)
                                                                                                          tCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCt                                        >  2:1488132/1‑148 (MQ=255)
                                                                                                            aCCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCtgt                                      >  1:2478689/1‑148 (MQ=255)
                                                                                                                gATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGa                                  >  2:1047800/1‑148 (MQ=255)
                                                                                                                gATGCCCGTACGGCGTGGTGTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGa                                  >  2:2147435/1‑148 (MQ=255)
                                                                                                                     ccGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCa                             <  2:2340684/148‑1 (MQ=255)
                                                                                                                     ccGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCa                             <  2:1702585/148‑1 (MQ=255)
                                                                                                                        tACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACg                          >  1:446819/1‑148 (MQ=255)
                                                                                                                          cGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACgag                        <  2:2300781/148‑1 (MQ=255)
                                                                                                                              gtggtgTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACgg                    <  2:224388/148‑1 (MQ=255)
                                                                                                                              gtggtgTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACgg                    <  2:3106592/148‑1 (MQ=255)
                                                                                                                              gtggtgTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACgg                    <  1:1948954/148‑1 (MQ=255)
                                                                                                                               tggtgTAACGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGt                   >  1:1427011/1‑148 (MQ=255)
                                                                                                                                    tAACGACACAATGCACACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTaaaa              <  1:1526650/148‑1 (MQ=255)
                                                                                                                                      aCGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAc            <  2:1948330/148‑1 (MQ=255)
                                                                                                                                       cGAGACAATGCTAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAca           <  1:809290/148‑1 (MQ=255)
                                                                                                                                          gaCAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAAcagcc        <  1:1440928/148‑1 (MQ=255)
                                                                                                                                                gCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCagcagc  >  1:1183005/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTGCCAGGGAGCGAAAAAGCGTTCATCTGACCACGGCCACAGCCAGCCAACGCCTTTACCGCCAGTGGTTACCGAATCCAGCAAGCTGTGCGATAGCAACGAGACGGTTAAAAACAGCCAGCAGC  >  CP009273/551456‑551747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑