Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 624,669 G→T 94.2% T221T (ACG→ACT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273624,6690GT94.2% 544.3 / 23.9 190T221T (ACG→ACT) entA2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (6/5);  new base T (93/86);  total (99/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

Only 100 of 190 total aligned reads displayed.

CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACA  >  CP009273/624524‑624812
                                                                                                                                                 |                                                                                                                                               
cGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAt                                                                                                                                               <  1:208050/148‑1 (MQ=255)
cGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAt                                                                                                                                               <  1:208046/148‑1 (MQ=255)
  ccTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                             <  1:1123857/148‑1 (MQ=255)
  ccTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAttt                                                                                                                                             >  2:330602/1‑148 (MQ=255)
   cTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTAtttt                                                                                                                                            >  2:1696606/1‑148 (MQ=255)
    tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTg                                                                                                                                           <  1:3541398/148‑1 (MQ=255)
    tGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTg                                                                                                                                           <  1:1782910/148‑1 (MQ=255)
      gCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                         >  2:3352243/1‑148 (MQ=255)
      gCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                         <  1:930365/148‑1 (MQ=255)
      gCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGtt                                                                                                                                         >  1:2525329/1‑148 (MQ=255)
         ccACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCt                                                                                                                                      <  2:829770/148‑1 (MQ=255)
             cgacaccgaTATGCAAAGCACGCTGTGGGTTAGCGATGACGCCGAAGAACAGCGTATTAGCGGCTTTGTCGAGCAGTTTAAAATAGGCATTCAGCTGGTGAAAATCGCCAGTCCACAAGAGATCGCCAAAACTATTATGTTCCTctac                                                                                                                                  >  1:2243409/1‑145 (MQ=255)
              gacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCct                                                                                                                                 <  1:2479784/148‑1 (MQ=255)
              gacaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCct                                                                                                                                 <  1:3411274/148‑1 (MQ=255)
               acaccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCctc                                                                                                                                >  2:1422425/1‑148 (MQ=255)
                caccgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCctct                                                                                                                               >  1:2327266/1‑148 (MQ=255)
                   cgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGAc                                                                                                                            >  1:46625/1‑148 (MQ=255)
                   cgaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGAc                                                                                                                            >  1:1966284/1‑148 (MQ=255)
                    gaTATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGAcc                                                                                                                           <  1:1276570/148‑1 (MQ=255)
                       atGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCg                                                                                                                        <  1:1559621/148‑1 (MQ=255)
                         gCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgcc                                                                                                                      >  2:3531364/1‑148 (MQ=255)
                             cgcacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCgccagcc                                                                                                                  >  2:2595462/1‑148 (MQ=255)
                               cacgcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCat                                                                                                                >  2:3292866/1‑148 (MQ=255)
                                  gcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATAtt                                                                                                             <  2:1083274/148‑1 (MQ=255)
                                  gcTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATAtt                                                                                                             <  2:3404891/148‑1 (MQ=255)
                                    tgtgGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTAc                                                                                                           >  2:569803/1‑148 (MQ=255)
                                       gggTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCt                                                                                                        <  1:2993469/148‑1 (MQ=255)
                                        ggTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTa                                                                                                       <  2:1191531/148‑1 (MQ=255)
                                        ggTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTa                                                                                                       >  1:3383298/1‑148 (MQ=255)
                                           gAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAg                                                                                                    >  2:1796135/1‑148 (MQ=255)
                                              cGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGat                                                                                                 >  1:406830/1‑148 (MQ=255)
                                                aTGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGatat                                                                                               <  1:925400/148‑1 (MQ=255)
                                                  gACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATtg                                                                                             <  1:300085/148‑1 (MQ=255)
                                                  gACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATtg                                                                                             >  2:528852/1‑148 (MQ=255)
                                                   aCGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGat                                                                                                 >  2:459589/1‑143 (MQ=255)
                                                   aCGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGat                                                                                                 <  1:459589/143‑1 (MQ=255)
                                                     gCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTgg                                                                                          <  1:821402/148‑1 (MQ=255)
                                                      ccGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGt                                                                                         <  2:399904/148‑1 (MQ=255)
                                                      ccGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGt                                                                                         >  2:3488746/1‑148 (MQ=255)
                                                         aagaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGa                                                                                      >  2:1295277/1‑148 (MQ=255)
                                                          agaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                     >  2:2055639/1‑148 (MQ=255)
                                                          agaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                     >  1:1062324/1‑148 (MQ=255)
                                                          agaaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGAt                                                                                     >  1:1062320/1‑148 (MQ=255)
                                                            aaCTGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                   <  2:1310954/148‑1 (MQ=255)
                                                            aaCAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATgg                                                                                   <  2:3080872/148‑1 (MQ=255)
                                                                 cGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTTCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCt                                                                              >  2:1815448/1‑148 (MQ=255)
                                                                      tCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACg                                                                         <  1:575865/148‑1 (MQ=255)
                                                                       cgcgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGc                                                                        <  1:2257038/148‑1 (MQ=255)
                                                                         cgGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTg                                                                      <  2:2561773/148‑1 (MQ=255)
                                                                           gCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTggg                                                                    >  1:1758704/1‑148 (MQ=255)
                                                                            cTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTgggg                                                                   >  2:166172/1‑148 (MQ=255)
                                                                              ttGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGc                                                                 >  1:2790191/1‑148 (MQ=255)
                                                                               tGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCa                                                                >  1:2463657/1‑148 (MQ=255)
                                                                                 gCGAGCAGTTTAAACTCGGCATTCCGCTTGGGAAAATCGCCCGTCCAAACTATATCGCCAACACTATTGTGTTCCTCGCATCTGACCTCGCCAGCCATATTACACTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATa                                                              >  2:1840568/1‑148 (MQ=255)
                                                                                 gCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATa                                                              <  1:324494/148‑1 (MQ=255)
                                                                                    aGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGc                                                           >  2:2992942/1‑148 (MQ=255)
                                                                                     gCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                          >  1:2171698/1‑148 (MQ=255)
                                                                                     gCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCa                                                          >  1:124387/1‑148 (MQ=255)
                                                                                      cAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATAGTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCAt                                                         >  2:1922961/1‑148 (MQ=255)
                                                                                        gTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGa                                                       <  2:2241263/148‑1 (MQ=255)
                                                                                        gTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGa                                                       <  2:2687390/148‑1 (MQ=255)
                                                                                         tttAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGAt                                                      <  1:2882389/148‑1 (MQ=255)
                                                                                           tAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCt                                                    >  1:1719443/1‑148 (MQ=255)
                                                                                             aaCTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTgg                                                  >  2:2149499/1‑148 (MQ=255)
                                                                                               cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaa                                                <  2:868590/148‑1 (MQ=255)
                                                                                               cTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGaa                                                >  1:732068/1‑148 (MQ=255)
                                                                                                  ggCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACg                                             >  1:2328991/1‑148 (MQ=255)
                                                                                                  ggCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACg                                             >  1:2329272/1‑148 (MQ=255)
                                                                                                       tCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCAtt                                        >  1:3005933/1‑148 (MQ=255)
                                                                                                         cGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTa                                      <  1:1422425/148‑1 (MQ=255)
                                                                                                          gCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTaa                                     <  1:2190427/148‑1 (MQ=255)
                                                                                                            tGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACg                                   <  2:1349140/148‑1 (MQ=255)
                                                                                                             ggggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGACACGCCATTTAACGc                                  >  2:477463/1‑148 (MQ=255)
                                                                                                             ggggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGc                                  >  1:3143348/1‑148 (MQ=255)
                                                                                                              gggAAAATCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCt                                 <  1:876943/148‑1 (MQ=255)
                                                                                                                 aaaaTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcga                              <  2:2244757/148‑1 (MQ=255)
                                                                                                                 aaaaTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATAACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcga                              >  2:3040164/1‑148 (MQ=255)
                                                                                                                    aTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcgacga                           <  2:704587/148‑1 (MQ=255)
                                                                                                                    aTCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTcgacga                           <  2:2784328/148‑1 (MQ=255)
                                                                                                                     tCGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGaa                          >  2:512216/1‑148 (MQ=255)
                                                                                                                     tCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGaa                          <  2:66607/148‑1 (MQ=255)
                                                                                                                      cGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAAc                         <  2:1263447/148‑1 (MQ=255)
                                                                                                                      cGCCCGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAAc                         >  2:1164493/1‑148 (MQ=255)
                                                                                                                         ccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGa                      <  1:2641693/148‑1 (MQ=255)
                                                                                                                         ccGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGa                      <  1:684277/148‑1 (MQ=255)
                                                                                                                          cGTCCACAAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGaa                     >  1:1299624/1‑148 (MQ=255)
                                                                                                                                caAGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccac               >  2:1534133/1‑148 (MQ=255)
                                                                                                                                 aaGAGATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccacc              <  2:143851/148‑1 (MQ=255)
                                                                                                                                  agagATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccacca             <  2:1473592/148‑1 (MQ=255)
                                                                                                                                  agagATCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGccacca             >  2:428352/1‑148 (MQ=255)
                                                                                                                                   gagaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAg            >  1:595369/1‑148 (MQ=255)
                                                                                                                                     gaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCg          <  1:1808848/148‑1 (MQ=255)
                                                                                                                                     gaTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCg          <  1:614730/148‑1 (MQ=255)
                                                                                                                                     gaTCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCg          >  1:334327/1‑148 (MQ=255)
                                                                                                                                      aTCGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGa         >  1:985663/1‑148 (MQ=255)
                                                                                                                                        cGCCAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATa       <  2:2074136/148‑1 (MQ=255)
                                                                                                                                           cAACACTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAaca    <  1:2398171/148‑1 (MQ=255)
                                                                                                                                             acacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca  <  1:2065879/148‑1 (MQ=255)
                                                                                                                                             acacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca  <  1:769766/148‑1 (MQ=255)
                                                                                                                                             acacTATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAAcaca  >  2:896214/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CGCCTGGCTCCACCGACACCGATATGCAACGCACGCTGTGGGTGAGCGATGACGCCGAAGAACAGCGTATTCGCGGCTTTGGCGAGCAGTTTAAACTCGGCATTCCGCTGGGGAAAATCGCCCGTCCACAAGAGATCGCCAACACGATTTTGTTCCTCGCCTCTGACCTCGCCAGCCATATTACCCTACAGGATATTGTGGTCGATGGCGGCTCAACGCTGGGGGCATAAGCATGATCTGGAAACGCCATTTAACGCTCGACGAACTGAACGCCACCAGCGATAACACA  >  CP009273/624524‑624812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑