Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 628,980 T→A 19.4% intergenic (‑47/‑62) ybdH ← / → ybdL putative oxidoreductase/methionine aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273628,9800TA19.4% 456.0 / 102.4 191intergenic (‑47/‑62)ybdH/ybdLputative oxidoreductase/methionine aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (78/76);  new base A (18/19);  total (96/95)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 193 total aligned reads displayed.

GTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTGGCGCAGCA  >  CP009273/628834‑629127
                                                                                                                                                  |                                                                                                                                                   
gttgttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGAGTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTg                                                                                                                                                    >  2:2761102/1‑148 (MQ=255)
  tgttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGcc                                                                                                                                                  >  1:2572773/1‑148 (MQ=255)
  tgttCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGcc                                                                                                                                                  >  2:259122/1‑148 (MQ=255)
       atcaGTGAAAAAATCGTGCAGGTGATTGACACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATa                                                                                                                                             <  2:2201758/148‑1 (MQ=255)
       atcaGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATa                                                                                                                                             >  2:885318/1‑148 (MQ=255)
        tcaGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATAc                                                                                                                                            <  2:2700594/148‑1 (MQ=255)
         caGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACt                                                                                                                                           >  1:2204429/1‑148 (MQ=255)
          aGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTa                                                                                                                                          <  1:2592927/148‑1 (MQ=255)
           gTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAg                                                                                                                                         >  1:1165603/1‑148 (MQ=255)
           gTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAg                                                                                                                                         >  1:754223/1‑148 (MQ=255)
            tGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGc                                                                                                                                        >  1:1163325/1‑148 (MQ=255)
             gAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCa                                                                                                                                       <  2:801417/148‑1 (MQ=255)
              aaaaaaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAGCGTCTTGATTGCCAAATACTAGCAt                                                                                                                                      <  1:673360/148‑1 (MQ=255)
              aaaaaaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCAt                                                                                                                                      >  2:479813/1‑148 (MQ=255)
              aaaaaaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCAt                                                                                                                                      <  1:673325/148‑1 (MQ=255)
              aaaaaaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCAt                                                                                                                                      <  2:343571/148‑1 (MQ=255)
               aaaaaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATc                                                                                                                                     <  2:1831477/148‑1 (MQ=255)
               aaaaaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATc                                                                                                                                     >  2:260823/1‑148 (MQ=255)
                 aaaTCGTGCAGGTGAATGAAACTTCCTCCATGTCAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATATGATTGTGATGCATGTTGTGTTGTCATTATTGTTTTATTTAGACATCTAAACGTCTTGATTGACAAATAATAGCATCgt                                                                                                                                   >  1:203221/1‑148 (MQ=255)
                  aaTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCgtg                                                                                                                                  <  2:206462/148‑1 (MQ=255)
                   agcGTGCAGGTGATTGAAACTTCCTGGATGTGAAAGGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCgtgt                                                                                                                                 >  2:1953338/3‑148 (MQ=255)
                   aTCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCgtgt                                                                                                                                 <  1:7828/148‑1 (MQ=255)
                    tCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGtt                                                                                                                                <  2:1636837/148‑1 (MQ=255)
                     cGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTta                                                                                                                               >  2:3163146/1‑148 (MQ=255)
                      gTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTtat                                                                                                                              <  2:1523515/148‑1 (MQ=255)
                      gTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTtat                                                                                                                              <  2:1499422/148‑1 (MQ=255)
                      gTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTtat                                                                                                                              <  2:3289412/148‑1 (MQ=255)
                       tGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTtata                                                                                                                             >  2:1618761/1‑148 (MQ=255)
                           ggTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGc                                                                                                                         <  2:2710165/148‑1 (MQ=255)
                           ggTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGc                                                                                                                         <  2:936151/148‑1 (MQ=255)
                            gTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGcc                                                                                                                        >  1:1472409/1‑148 (MQ=255)
                            gTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGcc                                                                                                                        >  1:2514189/1‑148 (MQ=255)
                                ttGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCa                                                                                                                    <  1:398817/148‑1 (MQ=255)
                                 tGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCaa                                                                                                                   >  1:1131112/1‑148 (MQ=255)
                                     aCTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACg                                                                                                               <  2:1377245/148‑1 (MQ=255)
                                     aCTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACg                                                                                                               >  1:1735173/1‑148 (MQ=255)
                                     aCTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACg                                                                                                               <  1:1574009/148‑1 (MQ=255)
                                      cTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGc                                                                                                              >  2:1787183/1‑148 (MQ=255)
                                        tgcTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCaa                                                                                                            >  1:483269/3‑148 (MQ=255)
                                        tCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCaa                                                                                                            >  2:1397566/1‑148 (MQ=255)
                                           tGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACtt                                                                                                         >  1:1099692/1‑148 (MQ=255)
                                              aTGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGt                                                                                                      >  1:434432/1‑148 (MQ=255)
                                                 tgAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAg                                                                                                   >  1:3387874/1‑148 (MQ=255)
                                                      agtagtTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACa                                                                                              >  1:1858807/1‑148 (MQ=255)
                                                           ttAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATaaa                                                                                         >  2:590698/1‑148 (MQ=255)
                                                             aGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATaaaaa                                                                                       <  2:1434689/148‑1 (MQ=255)
                                                             aGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATaaaaa                                                                                       <  1:1397566/148‑1 (MQ=255)
                                                             aGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATaaaaa                                                                                       <  2:2622988/148‑1 (MQ=255)
                                                                  ggCCGACGACCACGCGGATAGGATTGTGAGGAATGTTGTGTTGTCCTTATTGTTTTATTTATACATATAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACa                                                                                  <  1:3280793/148‑1 (MQ=255)
                                                                   gCCGACGACCACGCGGATAGGATTGTGAGGCATTTTGTGTTGTCCTTATTGTTTTATTTAGAAATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACaa                                                                                 <  1:3034873/148‑1 (MQ=255)
                                                                    ccGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACaaa                                                                                <  1:731960/148‑1 (MQ=255)
                                                                     cgacgaCCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAAt                                                                               <  2:433452/148‑1 (MQ=255)
                                                                        cgacCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAAc                                                                            >  1:911137/1‑148 (MQ=255)
                                                                        cgacCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTAAAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAAc                                                                            >  2:1580127/1‑148 (MQ=255)
                                                                                cgGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGAt                                                                    <  1:1884157/148‑1 (MQ=255)
                                                                                cgGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGAt                                                                    <  1:337192/148‑1 (MQ=255)
                                                                                 ggATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGAtt                                                                   <  1:2680158/148‑1 (MQ=255)
                                                                                  gATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTc                                                                  <  2:2636648/148‑1 (MQ=255)
                                                                                   aTAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTcc                                                                 >  2:2383194/1‑148 (MQ=255)
                                                                                    tAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCca                                                                <  1:2697369/148‑1 (MQ=255)
                                                                                    tAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCca                                                                <  2:766987/148‑1 (MQ=255)
                                                                                     aGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCcac                                                               >  1:2190341/1‑148 (MQ=255)
                                                                                        aTTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACaaa                                                            >  2:2181257/1‑148 (MQ=255)
                                                                                             gAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCaaa                                                       <  2:2103175/148‑1 (MQ=255)
                                                                                             gAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCaaa                                                       >  1:3184263/1‑148 (MQ=255)
                                                                                                gCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACtt                                                    >  1:110932/1‑148 (MQ=255)
                                                                                                    gttgtgttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCcac                                                >  2:2497879/1‑148 (MQ=255)
                                                                                                     ttgtgttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCcaca                                               >  1:3089833/1‑148 (MQ=255)
                                                                                                     ttgtgttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCcaca                                               <  2:2523457/148‑1 (MQ=255)
                                                                                                     ttgtgttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCcaca                                               <  1:2379912/148‑1 (MQ=255)
                                                                                                     ttgtgttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCcaca                                               >  2:1394731/1‑148 (MQ=255)
                                                                                                      tgtgttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACaa                                              <  2:1036582/148‑1 (MQ=255)
                                                                                                        tgttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACt                                            >  1:3364790/1‑148 (MQ=255)
                                                                                                          ttgtCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTg                                          >  2:1639075/1‑148 (MQ=255)
                                                                                                              ccTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCAACAACTTGGcac                                      >  2:2861054/1‑148 (MQ=255)
                                                                                                                ttATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGcacca                                    >  2:3510692/1‑148 (MQ=255)
                                                                                                                 tATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGcaccac                                   <  1:3409958/148‑1 (MQ=255)
                                                                                                                 tATTGTTTTATTTAGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGcaccac                                   >  1:1795918/1‑148 (MQ=255)
                                                                                                                   ttGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTa                                 <  1:1953338/148‑1 (MQ=255)
                                                                                                                       tttatttaGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTAtttt                             >  2:2039416/1‑148 (MQ=255)
                                                                                                                         tatttaGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCa                           <  2:1131112/148‑1 (MQ=255)
                                                                                                                         tatttaGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCa                           <  2:2935169/148‑1 (MQ=255)
                                                                                                                         tatttaGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCa                           >  1:954685/1‑148 (MQ=255)
                                                                                                                         tatttaGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCa                           >  1:2237608/1‑148 (MQ=255)
                                                                                                                         tatttaGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCa                           >  1:514359/1‑148 (MQ=255)
                                                                                                                         tatttaGACATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCa                           <  2:2372709/148‑1 (MQ=255)
                                                                                                                               gACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGa                     >  1:134727/1‑148 (MQ=255)
                                                                                                                               gACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGa                     >  1:1235212/1‑148 (MQ=255)
                                                                                                                                aCATCTAAACGTCTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGAt                    <  2:108386/148‑1 (MQ=255)
                                                                                                                                 cATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATg                   <  1:1618780/148‑1 (MQ=255)
                                                                                                                                   tCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAg                 <  2:3039603/148‑1 (MQ=255)
                                                                                                                                     tAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAgcg               >  2:952612/1‑148 (MQ=255)
                                                                                                                                       aaCGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAgcgcg             >  2:3128520/1‑148 (MQ=255)
                                                                                                                                       aaCGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAgcgcg             >  2:3128046/1‑148 (MQ=255)
                                                                                                                                       aaCGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAgcgcg             >  1:3357128/1‑148 (MQ=255)
                                                                                                                                        aCGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAgcgcgc            >  2:3344421/1‑148 (MQ=255)
                                                                                                                                           tCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTgg         >  2:120466/1‑148 (MQ=255)
                                                                                                                                           tCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTgg         >  1:3036908/1‑148 (MQ=255)
                                                                                                                                            cTTGATAGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTGgc        >  1:2858386/1‑148 (MQ=255)
                                                                                                                                                  tGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTGGCgcagca  >  1:2905806/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTTGTTCATCAGTGAAAAAATCGTGCAGGTGATTGAAACTTCCTGGATGTGAAAAGTAGTTAGCCGGGCCGACGACCACGCGGATAGGATTGTGAGGCATGTTGTGTTGTCCTTATTGTTTTATTTAGACATCTAAACGTCTTGATTGCCAAATACTAGCATCGTGTTATAGTGCCTTCAACACGCAACTTCGTCAGGTACAATAAAAATGACAAATAACCCTCTGATTCCACAAAGCAAACTTCCACAACTTGGCACCACTATTTTCACCCAGATGAGCGCGCTGGCGCAGCA  >  CP009273/628834‑629127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑