Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 639,231 G→A 100% L65F (CTT→TTT)  rnk ← regulator of nucleoside diphosphate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273639,2310GA99.6% 763.3 / ‑4.3 231L65F (CTT→TTT) rnkregulator of nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (121/109);  minor base C (1/0);  total (122/109)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

CCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATAT  >  CP009273/639086‑639374
                                                                                                                                                 |                                                                                                                                               
  cGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTg                                                                                                                                             <  2:1408148/148‑1 (MQ=255)
   ggAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGc                                                                                                                                            >  2:624633/1‑148 (MQ=255)
      aGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGa                                                                                                                                         <  1:401737/148‑1 (MQ=255)
       gTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                        >  2:608506/1‑148 (MQ=255)
       gTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGaa                                                                                                                                        <  2:2511988/148‑1 (MQ=255)
          cccAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                     >  1:558167/1‑148 (MQ=255)
          cccAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAAttt                                                                                                                                     <  2:1437179/148‑1 (MQ=255)
           ccAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTa                                                                                                                                    <  2:3272750/148‑1 (MQ=255)
           ccAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTa                                                                                                                                    <  2:3109694/148‑1 (MQ=255)
            cAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTaa                                                                                                                                   <  2:2215693/148‑1 (MQ=255)
             aGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAc                                                                                                                                  <  1:1347514/148‑1 (MQ=255)
             aGTGAATGGAATCGCCAACGAGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAAc                                                                                                                                  <  2:290868/148‑1 (MQ=255)
                gAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCg                                                                                                                               >  2:1169144/1‑148 (MQ=255)
                gAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCg                                                                                                                               >  2:1170526/1‑148 (MQ=255)
                 aaTGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCgg                                                                                                                              <  2:1600446/148‑1 (MQ=255)
                   tGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCt                                                                                                                            <  2:3405379/148‑1 (MQ=255)
                   tGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCt                                                                                                                            >  2:1304685/1‑148 (MQ=255)
                   tGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCt                                                                                                                            >  1:211275/1‑148 (MQ=255)
                        tCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCa                                                                                                                       >  2:389568/1‑148 (MQ=255)
                         cGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCGGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAt                                                                                                                      >  1:1283839/1‑148 (MQ=255)
                         cGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCAt                                                                                                                      >  1:221146/1‑148 (MQ=255)
                            cAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGt                                                                                                                   >  2:2080354/1‑148 (MQ=255)
                            cAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGt                                                                                                                   <  1:1418012/148‑1 (MQ=255)
                             aaCGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTc                                                                                                                  <  2:1797573/148‑1 (MQ=255)
                                   cAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcg                                                                                                            <  2:2777446/148‑1 (MQ=255)
                                   cAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcg                                                                                                            <  1:3041382/148‑1 (MQ=255)
                                      ccccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcg                                                                                                         >  2:281011/1‑148 (MQ=255)
                                      ccccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCAcgtcg                                                                                                         >  2:3128381/1‑148 (MQ=255)
                                        ccAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCgtg                                                                                                       >  2:588812/1‑148 (MQ=255)
                                            cagTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGc                                                                                                   <  2:881129/148‑1 (MQ=255)
                                            cagTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGc                                                                                                   >  1:2289235/1‑148 (MQ=255)
                                             agTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCa                                                                                                  <  1:1132599/148‑1 (MQ=255)
                                               tGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTCTTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtc                                                                                                >  2:3344233/1‑148 (MQ=255)
                                               tGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtc                                                                                                >  1:3125762/1‑148 (MQ=255)
                                               tGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtc                                                                                                >  1:1932418/1‑148 (MQ=255)
                                               tGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtc                                                                                                >  2:1585071/1‑148 (MQ=255)
                                                 cGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtctc                                                                                              <  1:968261/148‑1 (MQ=255)
                                                  ggCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATctct                                                                                             <  2:1371807/148‑1 (MQ=255)
                                                   gCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCtctt                                                                                            <  2:739309/148‑1 (MQ=255)
                                                      ccTCCCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTg                                                                                         <  2:2137789/148‑1 (MQ=255)
                                                        tACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGc                                                                                       >  2:2181892/1‑148 (MQ=255)
                                                           cGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGaa                                                                                    <  1:1019534/148‑1 (MQ=255)
                                                              aGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGAGAacac                                                                                 >  2:3330726/1‑148 (MQ=255)
                                                                ccATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAt                                                                               <  1:2170684/148‑1 (MQ=255)
                                                                  aTAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACAttt                                                                             >  2:3386524/1‑148 (MQ=255)
                                                                   tAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTg                                                                            >  1:681353/1‑148 (MQ=255)
                                                                   tAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTg                                                                            >  1:2261878/1‑148 (MQ=255)
                                                                   tAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTg                                                                            >  1:1210437/1‑148 (MQ=255)
                                                                    aaCGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTgg                                                                           >  1:1994782/1‑148 (MQ=255)
                                                                     aCGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTggg                                                                          >  2:1862236/1‑148 (MQ=255)
                                                                      cGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGgc                                                                         >  1:1596641/1‑148 (MQ=255)
                                                                       ggAAAGCTGAGTAGTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACTCTCTTCGCCATCGCTAAAATTGCGGAATTTAAACCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGgcg                                                                        <  1:3454400/148‑1 (MQ=255)
                                                                         aaaGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGcgcg                                                                      >  2:3204741/1‑148 (MQ=255)
                                                                         aaaGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGcgcg                                                                      >  2:2889480/1‑148 (MQ=255)
                                                                          aaGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGa                                                                     <  2:37481/148‑1 (MQ=255)
                                                                               gAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCaa                                                                <  2:1010966/148‑1 (MQ=255)
                                                                                  tATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAActc                                                             >  2:792322/1‑148 (MQ=255)
                                                                                     tGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGTCGAACACATTTGGGCGCGATCCAACTCTGc                                                          >  1:3418528/1‑148 (MQ=255)
                                                                                      gCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCg                                                         <  2:822924/148‑1 (MQ=255)
                                                                                      gCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCg                                                         <  2:3030527/148‑1 (MQ=255)
                                                                                       cTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGt                                                        <  1:1837906/148‑1 (MQ=255)
                                                                                         aTCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGttt                                                      >  1:1734313/1‑148 (MQ=255)
                                                                                              tCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAAcgc                                                 >  2:1470266/1‑148 (MQ=255)
                                                                                                aTTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGt                                               <  1:3105569/148‑1 (MQ=255)
                                                                                                 tttttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTc                                              >  1:1402935/1‑148 (MQ=255)
                                                                                                 tttttGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTc                                              >  1:1035470/1‑148 (MQ=255)
                                                                                                       ccGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAt                                        <  1:1322820/148‑1 (MQ=255)
                                                                                                       ccGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATCGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGAt                                        >  2:1646041/1‑148 (MQ=255)
                                                                                                            tACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCa                                   <  1:3422866/148‑1 (MQ=255)
                                                                                                            tACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCa                                   <  1:3500734/148‑1 (MQ=255)
                                                                                                             acacCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGaaa                                  >  2:3515986/1‑148 (MQ=255)
                                                                                                              cacCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCaaa                                 <  1:3190340/148‑1 (MQ=255)
                                                                                                               acCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCAtctc                                                                                              <  2:672411/86‑1 (MQ=255)
                                                                                                               acCAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAAc                                >  1:128782/1‑148 (MQ=255)
                                                                                                                ccAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAAcc                               >  1:2631391/1‑148 (MQ=255)
                                                                                                                 cAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCa                              >  2:2847273/1‑148 (MQ=255)
                                                                                                                 cAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCa                              >  1:1690380/1‑148 (MQ=255)
                                                                                                                 cAGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCa                              <  2:258766/148‑1 (MQ=255)
                                                                                                                  aGCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAg                             <  1:3242975/148‑1 (MQ=255)
                                                                                                                   gCGTGCGCACACTCACTTCGCAATCTCTAAAATTGCGTAATTTATCCCGGCTGTTCATTGTCACCACGTCGTGTGTCATCTCTTCTGGCGAACACATTTTGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGc                            <  2:3113520/148‑1 (MQ=255)
                                                                                                                   gCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGc                            <  1:2476894/148‑1 (MQ=255)
                                                                                                                   gCGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGc                            <  2:1465679/148‑1 (MQ=255)
                                                                                                                    cGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCa                           >  2:2277290/1‑148 (MQ=255)
                                                                                                                    cGTGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGAATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCa                           <  1:3073297/148‑1 (MQ=255)
                                                                                                                      tGCGCACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATa                         <  1:624633/148‑1 (MQ=255)
                                                                                                                       gcgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAg                        >  2:25888/1‑148 (MQ=255)
                                                                                                                       gcgcACACGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAg                        <  2:3139340/148‑1 (MQ=255)
                                                                                                                           acacGCACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCGTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCgg                    <  1:1355411/148‑1 (MQ=255)
                                                                                                                                cACTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGc               <  2:126501/148‑1 (MQ=255)
                                                                                                                                 aCTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCt              <  2:27570/148‑1 (MQ=255)
                                                                                                                                  cTTCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTc             <  1:1170318/148‑1 (MQ=255)
                                                                                                                                    tCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCca           >  1:289690/1‑148 (MQ=255)
                                                                                                                                    tCGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCca           >  1:723293/1‑148 (MQ=255)
                                                                                                                                     cGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCcag          >  2:529034/1‑148 (MQ=255)
                                                                                                                                     cGCCATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCcag          >  2:2316368/1‑148 (MQ=255)
                                                                                                                                       ccATCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGGCTAGGCGGGTTGCTCcagca        >  1:1534427/1‑148 (MQ=255)
                                                                                                                                         aTCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGa      >  1:2069169/1‑148 (MQ=255)
                                                                                                                                          tCGCTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGaa     >  2:1714419/1‑148 (MQ=255)
                                                                                                                                             cTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAatat  <  2:221111/148‑1 (MQ=255)
                                                                                                                                             cTAAAATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAatat  >  2:2258573/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCCGGAAGTTCCCAGTGAATGGAATCGCCAACGCGCAGCCCCAGCAGTGCGGCACCTACCGGAGCCATAACGGAAAGCTGAGTATTGCTATCGGTCATTTTTGCCGGATACACCAGCGTGCGCACACGCACTTCGCCATCGCTAAGATTGCGGAATTTAACCCGGCTGTTCATTGTCACCACGTCGTGTGGCATCTCTTCTGGCGAACACATTTGGGCGCGATCCAACTCTGCGTTTAACGCGTCGGCGATTGGCAAACCAGCATAGGCGGGTTGCTCCAGCAGAATAT  >  CP009273/639086‑639374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑