Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 647,234 C→T 100% E27K (GAA→AAA)  citC ← [citrate [pro‑3S]‑lyase] ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273647,2340CT97.9% 928.5 / 7.0 282E27K (GAA→AAA) citC[citrate [pro‑3S]‑lyase] ligase
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base T (135/141);  total (140/142)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 285 total aligned reads displayed.

GCCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAA  >  CP009273/647092‑647378
                                                                                                                                              |                                                                                                                                                
     tCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAAt                                                                                                                                        >  1:1001121/1‑148 (MQ=255)
        ttCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTgg                                                                                                                                     >  1:2551997/1‑148 (MQ=255)
        ttCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTgg                                                                                                                                     >  1:1876521/1‑148 (MQ=255)
         tCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTggg                                                                                                                                    <  2:2406217/148‑1 (MQ=255)
            ccGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGa                                                                                                                                 >  2:261701/1‑148 (MQ=255)
             cgcgGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGAt                                                                                                                                >  2:2473714/1‑148 (MQ=255)
                ggacggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTc                                                                                                                             >  1:1252184/1‑148 (MQ=255)
                 gacggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTcc                                                                                                                            >  1:3168766/1‑148 (MQ=255)
                  acggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCg                                                                                                                           >  1:2348539/1‑148 (MQ=255)
                  acggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCg                                                                                                                           >  2:1446007/1‑148 (MQ=255)
                  acggaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCg                                                                                                                           >  2:857442/1‑148 (MQ=255)
                     gaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCa                                                                                                                        <  2:656387/148‑1 (MQ=255)
                     gaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCa                                                                                                                        >  2:1536010/1‑148 (MQ=255)
                     gaTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCa                                                                                                                        <  1:1976841/148‑1 (MQ=255)
                     gaTTCACTGATAGCAACGCATTTAATAATATGTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCa                                                                                                                        <  2:2934256/148‑1 (MQ=255)
                      aTTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCAt                                                                                                                       >  2:424228/1‑148 (MQ=255)
                       ttCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCAtt                                                                                                                      <  1:273135/148‑1 (MQ=255)
                       ttCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCAtt                                                                                                                      <  1:2068398/148‑1 (MQ=255)
                         cACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCAtttt                                                                                                                    <  2:2756418/148‑1 (MQ=255)
                              aTAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAt                                                                                                               >  2:2759370/1‑148 (MQ=255)
                                aGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAttt                                                                                                             >  2:2362555/1‑148 (MQ=255)
                                 gCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTAtttt                                                                                                            <  2:760607/148‑1 (MQ=255)
                                  cAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGACTTGGGCGATTTCCGCCATTTTTTTATTTTc                                                                                                           >  1:680339/1‑148 (MQ=255)
                                       cATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCACCATTTTTTTATTTTCTGAAc                                                                                                      >  2:257833/1‑148 (MQ=255)
                                         tttAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGt                                                                                                    <  1:2691166/148‑1 (MQ=255)
                                          ttAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGtt                                                                                                   <  1:218233/148‑1 (MQ=255)
                                            aataatATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGtttt                                                                                                 >  1:737118/1‑148 (MQ=255)
                                             ataataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTa                                                                                                >  1:2594586/1‑148 (MQ=255)
                                             ataataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTa                                                                                                <  1:2511342/148‑1 (MQ=255)
                                             ataataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTa                                                                                                <  1:1266439/148‑1 (MQ=255)
                                              taataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTAc                                                                                               >  2:287037/1‑148 (MQ=255)
                                              taataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTAc                                                                                               >  2:1072729/1‑148 (MQ=255)
                                              taataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTAc                                                                                               >  1:3245325/1‑148 (MQ=255)
                                              taataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTAc                                                                                               >  1:1724159/1‑148 (MQ=255)
                                               aataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTAcg                                                                                              <  2:315859/148‑1 (MQ=255)
                                               aataTTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTAcg                                                                                              >  1:45207/1‑148 (MQ=255)
                                                  atTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCgg                                                                                           <  2:1741098/148‑1 (MQ=255)
                                                   tttCCGGCAATTCCACCGCACGCGATAATCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCggg                                                                                          <  1:1938695/148‑1 (MQ=255)
                                                   tttCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCggg                                                                                          <  1:776825/148‑1 (MQ=255)
                                                        ggCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGaaa                                                                                     <  1:2011851/148‑1 (MQ=255)
                                                           aaTTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCTATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAata                                                                                  <  1:2914785/148‑1 (MQ=255)
                                                            aTTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAatat                                                                                 >  1:563511/1‑148 (MQ=255)
                                                            aTTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAatat                                                                                 >  1:562774/1‑148 (MQ=255)
                                                              tCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCa                                                                               <  1:1665219/148‑1 (MQ=255)
                                                              tCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCa                                                                               <  2:552419/148‑1 (MQ=255)
                                                               ccACCGCACGCGATCAGCTTTTCATCGCGGGTTACGGTAATAAAGACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCAt                                                                              <  2:3278036/148‑1 (MQ=255)
                                                               ccACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCAt                                                                              <  1:823977/148‑1 (MQ=255)
                                                                cACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCAtt                                                                             >  1:1209302/1‑148 (MQ=255)
                                                                 aCCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACTCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTg                                                                            <  1:1599835/148‑1 (MQ=255)
                                                                  ccGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGc                                                                           >  1:853971/1‑148 (MQ=255)
                                                                   cGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGcc                                                                          <  2:1675107/148‑1 (MQ=255)
                                                                           aTAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataa                                                                  <  1:2848892/148‑1 (MQ=255)
                                                                             aaGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACataata                                                                <  1:1222360/148‑1 (MQ=255)
                                                                              aGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACAtaataa                                                               >  1:3518132/1‑148 (MQ=255)
                                                                               gCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaa                                                              >  1:1068870/1‑148 (MQ=255)
                                                                               gCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaa                                                              >  1:282945/1‑148 (MQ=255)
                                                                                 ttttCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaata                                                            <  2:3077626/148‑1 (MQ=255)
                                                                                 ttttCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATaataaata                                                            <  1:552326/148‑1 (MQ=255)
                                                                                   ttCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGt                                                          >  2:3555552/1‑148 (MQ=255)
                                                                                   ttCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGt                                                          <  2:2324961/148‑1 (MQ=255)
                                                                                     cATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTAt                                                        <  2:503222/148‑1 (MQ=255)
                                                                                     cATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTAt                                                        >  2:3302273/1‑148 (MQ=255)
                                                                                      aTCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTAAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATc                                                       <  1:2399267/148‑1 (MQ=255)
                                                                                       tCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATcc                                                      <  1:1377020/148‑1 (MQ=255)
                                                                                       tCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATcc                                                      >  2:3127804/1‑148 (MQ=255)
                                                                                        cgcgGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCt                                                     <  1:3356048/148‑1 (MQ=255)
                                                                                          cgGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGa                                                   >  1:1141919/1‑148 (MQ=255)
                                                                                            ggTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAg                                                 >  1:72317/1‑148 (MQ=255)
                                                                                             gTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAgg                                                >  2:2041538/1‑148 (MQ=255)
                                                                                             gTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAgg                                                <  2:3211038/148‑1 (MQ=255)
                                                                                             gTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAgg                                                >  2:1311079/1‑148 (MQ=255)
                                                                                               tACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTg                                              <  1:2613147/148‑1 (MQ=255)
                                                                                                   gTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtg                                          >  2:700883/1‑148 (MQ=255)
                                                                                                      ataaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttg                                       >  1:3010429/1‑148 (MQ=255)
                                                                                                      ataaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttg                                       <  1:1503855/148‑1 (MQ=255)
                                                                                                       taaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCAtgttgt                                      >  2:1537914/1‑148 (MQ=255)
                                                                                                        aaataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATgttgtt                                     >  1:9476/1‑148 (MQ=255)
                                                                                                          ataCTTCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTAt                                   <  1:1758403/148‑1 (MQ=255)
                                                                                                               tCGACTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGAtt                              <  1:3420763/148‑1 (MQ=255)
                                                                                                                   cTGTGGTGTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCa                          <  2:1123642/148‑1 (MQ=255)
                                                                                                                      tggtgTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACg                       >  2:1944695/1‑148 (MQ=255)
                                                                                                                      tggtgTCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACg                       >  1:256427/1‑148 (MQ=255)
                                                                                                                      tggtgTCAACGCTAAAATCATTTTTATGCAGTAATTGGGCGATTTCCGCCATATTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATACTAAATAGTATCCTGTAGGTGCATGTTGTTATCTATTTGCAACg                       >  1:2249797/1‑148 (MQ=255)
                                                                                                                         tgtCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAt                    >  1:584947/1‑148 (MQ=255)
                                                                                                                         tgtCAACGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAt                    >  2:3328433/1‑148 (MQ=255)
                                                                                                                             aaCGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAAtgttg                <  2:1191695/148‑1 (MQ=255)
                                                                                                                               cGCTCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATgttgtt              >  1:21386/1‑148 (MQ=255)
                                                                                                                                  tCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCaa           <  1:2379418/148‑1 (MQ=255)
                                                                                                                                  tCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCaa           <  1:1230672/148‑1 (MQ=255)
                                                                                                                                  tCAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCaa           >  1:1409468/1‑148 (MQ=255)
                                                                                                                                   cAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAAt          >  2:2522894/1‑148 (MQ=255)
                                                                                                                                   cAAATCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGAATGTTGTTATAGATTTGAAACGAATGTTGTTCAAt          >  2:2688047/1‑148 (MQ=255)
                                                                                                                                    aaaTCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATg         >  2:1643404/1‑148 (MQ=255)
                                                                                                                                    aaaTCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATg         >  2:2536779/1‑148 (MQ=255)
                                                                                                                                       tCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTg      <  2:1252187/148‑1 (MQ=255)
                                                                                                                                       tCATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTg      <  2:2422880/148‑1 (MQ=255)
                                                                                                                                        cATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGc     >  2:323048/1‑148 (MQ=255)
                                                                                                                                        cATTTTTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGc     >  1:472099/1‑148 (MQ=255)
                                                                                                                                          ttttTATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCaa   <  1:3402751/148‑1 (MQ=255)
                                                                                                                                           ttttATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCaaa  <  2:1575188/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GCCAGTCCTTCACCGCGGACGGATTCACTGATAGCAACGCATTTAATAATATTTCCGGCAATTCCACCGCACGCGATAAGCTTTTCATCGCGGGTTACGGTAATAAATACTTCGACTGTGGTGTCAACGCTCAAATCATTTTCATGCAGGAATTGGGCGATTTCCGCCATTTTTTTATTTTCTGAACGTTTTACGCGGGTGAAAATATCATTGCCGAACATAATAAATAGTATCCTGAAGGTGCATGTTGTTATCGATTTGCAACGAATGTTGTTCAATGTTGCAAA  >  CP009273/647092‑647378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑