Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 658,096 C→A 33.9% M1M (ATG→ATT) † ybeD ← UPF0250 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273658,0960CA33.9% 301.5 / 156.2 191M1M (ATG→ATT) †ybeDUPF0250 family protein
Reads supporting (aligned to +/- strand):  ref base C (55/71);  new base A (37/28);  total (92/99)
Fisher's exact test for biased strand distribution p-value = 9.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 195 total aligned reads displayed.

GCTTGGTTTTACCGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTA  >  CP009273/657949‑658243
                                                                                                                                                   |                                                                                                                                                   
gctTGGTTTTACCGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTc                                                                                                                                                     >  1:793601/1‑148 (MQ=255)
    ggTTTTACCGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGt                                                                                                                                                 <  2:70315/148‑1 (MQ=255)
       tttACCGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAg                                                                                                                                              <  2:310102/148‑1 (MQ=255)
        ttACCGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGc                                                                                                                                             <  1:3384168/148‑1 (MQ=255)
        ttACCGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGc                                                                                                                                             >  1:460376/1‑148 (MQ=255)
         tACCGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCt                                                                                                                                            <  2:1615794/148‑1 (MQ=255)
           ccGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTcc                                                                                                                                          <  1:1022396/148‑1 (MQ=255)
                  ggTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGcgtaa                                                                                                                                   >  1:1930782/1‑148 (MQ=255)
                  ggTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGcgtaa                                                                                                                                   >  2:3176081/1‑148 (MQ=255)
                        gTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAAt                                                                                                                             >  1:3025341/1‑148 (MQ=255)
                           aCCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTaa                                                                                                                          <  2:807109/148‑1 (MQ=255)
                           aCCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTaa                                                                                                                          <  1:1574781/148‑1 (MQ=255)
                            ccTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTaaa                                                                                                                         <  1:1089924/148‑1 (MQ=255)
                                gcgcATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGc                                                                                                                     >  1:52690/1‑148 (MQ=255)
                                    ttGGCGCTGTACCAATTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACt                                                                                                                 <  2:1393575/147‑1 (MQ=255)
                                        cgcTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCg                                                                                                             >  2:898901/1‑148 (MQ=255)
                                            gTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAg                                                                                                         >  1:3337881/1‑148 (MQ=255)
                                               ccACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAg                                                                                                      <  2:1917968/148‑1 (MQ=255)
                                                  cTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCg                                                                                                   >  2:1256035/1‑148 (MQ=255)
                                                   ttCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCgg                                                                                                  <  2:2948266/148‑1 (MQ=255)
                                                    tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggg                                                                                                 <  2:1921902/148‑1 (MQ=255)
                                                    tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggg                                                                                                 <  2:732491/148‑1 (MQ=255)
                                                    tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggg                                                                                                 >  1:1502183/1‑148 (MQ=255)
                                                    tCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCggg                                                                                                 >  2:1607827/1‑148 (MQ=255)
                                                       accaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGt                                                                                              >  2:3385803/1‑148 (MQ=255)
                                                        ccaccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGtt                                                                                             >  2:2489641/1‑148 (MQ=255)
                                                         caccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttt                                                                                            >  1:478873/1‑148 (MQ=255)
                                                         caccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttt                                                                                            <  1:326472/148‑1 (MQ=255)
                                                         caccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttt                                                                                            >  2:982041/1‑148 (MQ=255)
                                                           ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                                                                          <  2:991966/148‑1 (MQ=255)
                                                           ccTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGttttt                                                                                          >  2:2055593/1‑148 (MQ=255)
                                                             tGATCACCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTa                                                                                        <  2:953734/148‑1 (MQ=255)
                                                              gATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTAc                                                                                       <  1:2270504/148‑1 (MQ=255)
                                                                tCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTg                                                                                     >  2:1858243/1‑148 (MQ=255)
                                                                   aCCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCACGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATg                                                                                  <  1:3458882/148‑1 (MQ=255)
                                                                    ccAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGc                                                                                 <  2:1518644/148‑1 (MQ=255)
                                                                    ccAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGc                                                                                 >  1:123658/1‑148 (MQ=255)
                                                                    ccAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGc                                                                                 <  2:3281345/148‑1 (MQ=255)
                                                                      aGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCtt                                                                               <  2:1271144/148‑1 (MQ=255)
                                                                       gCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTa                                                                              <  2:3254656/148‑1 (MQ=255)
                                                                         tCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGt                                                                            >  2:997705/1‑148 (MQ=255)
                                                                          cAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGta                                                                           <  1:2564453/148‑1 (MQ=255)
                                                                           aGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTCGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGtat                                                                          <  2:1948047/148‑1 (MQ=255)
                                                                            ggTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGtata                                                                         <  1:736556/148‑1 (MQ=255)
                                                                               aaCGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgg                                                                      <  1:2748927/148‑1 (MQ=255)
                                                                                aCGCCTGCCCCATAACTATGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATggg                                                                     <  2:331896/148‑1 (MQ=255)
                                                                                 cGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgggg                                                                    <  1:917064/148‑1 (MQ=255)
                                                                                 cGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgggg                                                                    <  1:333971/148‑1 (MQ=255)
                                                                                 cGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgggg                                                                    <  1:25343/148‑1 (MQ=255)
                                                                                 cGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATgggg                                                                    >  1:3529172/1‑148 (MQ=255)
                                                                                     tGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACgg                                                                >  1:2807577/1‑148 (MQ=255)
                                                                                             aaCTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTaca                                                        >  1:611675/1‑148 (MQ=255)
                                                                                             aaCTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTaca                                                        >  1:611680/1‑148 (MQ=255)
                                                                                              aCTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTacac                                                       >  2:3471599/1‑148 (MQ=255)
                                                                                              aCTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTacac                                                       <  1:2863214/148‑1 (MQ=255)
                                                                                               cTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACt                                                      >  1:1031568/1‑148 (MQ=255)
                                                                                                 ttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                    >  2:1175046/1‑148 (MQ=255)
                                                                                                 ttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                    >  2:1480570/1‑148 (MQ=255)
                                                                                                 ttGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACttt                                                    >  2:722790/1‑148 (MQ=255)
                                                                                                   gtaagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCa                                                  <  1:3557419/148‑1 (MQ=255)
                                                                                                    taagtaaAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCaa                                                 <  1:2353962/148‑1 (MQ=255)
                                                                                                         aaaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGtt                                            <  1:2232886/148‑1 (MQ=255)
                                                                                                          aaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGttt                                           >  1:14057/1‑148 (MQ=255)
                                                                                                          aaaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGttt                                           >  1:12928/1‑148 (MQ=255)
                                                                                                           aaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTa                                          <  2:2645581/148‑1 (MQ=255)
                                                                                                           aaGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTa                                          <  2:1502146/148‑1 (MQ=255)
                                                                                                             ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAt                                        >  1:1706734/1‑148 (MQ=255)
                                                                                                             ggAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAt                                        <  1:2451729/148‑1 (MQ=255)
                                                                                                               aGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAttt                                      <  2:1810326/148‑1 (MQ=255)
                                                                                                                 gaGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAAttttt                                    <  2:1062089/147‑1 (MQ=255)
                                                                                                                    ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                 >  2:2170472/1‑148 (MQ=255)
                                                                                                                    ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                 <  2:3073614/148‑1 (MQ=255)
                                                                                                                    ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTAAGCTCCGGCGTAACGGAATTAAATAGCAACACACGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGACATTACACTTTCAAGAGTTTAATTTTTaac                                 >  1:2423043/1‑148 (MQ=255)
                                                                                                                    ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGACATTACACTTTCAAGTGTTTAATTTTTaac                                 >  2:2770880/1‑148 (MQ=255)
                                                                                                                    ggAATTCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGGATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaac                                 >  1:927944/1‑148 (MQ=255)
                                                                                                                     gAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaacc                                <  2:1673629/148‑1 (MQ=255)
                                                                                                                       aTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaa                              <  1:2055593/148‑1 (MQ=255)
                                                                                                                       aTTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaa                              <  1:1726015/148‑1 (MQ=255)
                                                                                                                         tCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaac                            >  2:3139401/1‑148 (MQ=255)
                                                                                                                         tCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTaaccaaac                            >  2:3553359/1‑148 (MQ=255)
                                                                                                                         tCAAGCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGATTAGTATATGGGGACGGACATTACACTTTCAAGTGTTTAATTTTTaaccaaac                            >  2:3335793/1‑148 (MQ=255)
                                                                                                                             gCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTac                                                         <  1:985183/115‑1 (MQ=255)
                                                                                                                             gCAGTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGt                        <  1:1541301/148‑1 (MQ=255)
                                                                                                                               aGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGa                      <  1:3476305/148‑1 (MQ=255)
                                                                                                                                gTTCGTTAAGTTTGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGAt                     <  2:460376/148‑1 (MQ=255)
                                                                                                                                 ttCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATg                    >  1:635586/1‑148 (MQ=255)
                                                                                                                                   cGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGa                  >  1:1190943/1‑148 (MQ=255)
                                                                                                                                    gTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGaa                 <  2:3461067/148‑1 (MQ=255)
                                                                                                                                     ttAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAAc                >  1:419544/1‑148 (MQ=255)
                                                                                                                                         gTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAtta            >  1:1924726/1‑148 (MQ=255)
                                                                                                                                          tttGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaa           <  2:835069/148‑1 (MQ=255)
                                                                                                                                          tttGGTTGTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaa           <  2:2560979/148‑1 (MQ=255)
                                                                                                                                            tGGTTTTAATTTCAGCTCCTGCTTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatt         <  1:2481821/148‑1 (MQ=255)
                                                                                                                                            tGGTTTTAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatt         <  1:2481835/148‑1 (MQ=255)
                                                                                                                                              gTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaattta       >  2:1773375/1‑148 (MQ=255)
                                                                                                                                               ttttAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaa      >  2:2901756/1‑148 (MQ=255)
                                                                                                                                               ttttAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaa      >  2:2901490/1‑148 (MQ=255)
                                                                                                                                                tttCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaat     >  2:1969957/1‑148 (MQ=255)
                                                                                                                                                tttAATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACAttaatttaat     >  2:3027761/1‑148 (MQ=255)
                                                                                                                                                  tCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATTGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAAACAAACCACTGATGGAAAATTAATTTAATGt   >  2:1210939/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GCTTGGTTTTACCGTTGGGGTGTAGTCACCTGGCGCATGGCGCTGTACCACTTCAACCACCTGATCAACCAGCTCAGGTAACGCCTGCCCCATAACTTTGTAAGTAAAAGGAGTAGGGAATTCAAGCAGTTCGTTAAGTTTGGTTTTCATGTCAGCTCCGGCGTAACGTAATTAAATAGCAACTCCCGCCAGAAGGCGGGAGTTTTTTACTGATGCTTAGTATATGGGGACGGAAATTACACTTTCAAGTGTTTAATTTTTAACCAAACCAGTGATGGAACATTAATTTAATGTA  >  CP009273/657949‑658243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑