Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,108 A→T 100% V522V (GTT→GTA)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,1080AT100.0% 780.9 / NA 234V522V (GTT→GTA) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (125/109);  total (125/109)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

GGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCA  >  CP009273/661963‑662252
                                                                                                                                                 |                                                                                                                                                
ggTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTac                                                                                                                                                >  1:1987078/1‑148 (MQ=255)
 gTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTaca                                                                                                                                               >  1:169832/1‑148 (MQ=255)
     cGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGt                                                                                                                                           >  2:217169/1‑148 (MQ=255)
       tAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTAc                                                                                                                                         >  2:2147615/1‑148 (MQ=255)
        aaaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACa                                                                                                                                        >  2:222132/1‑148 (MQ=255)
        aaaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACa                                                                                                                                        >  1:1639017/1‑148 (MQ=255)
         aaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAt                                                                                                                                       >  2:3501086/1‑148 (MQ=255)
          aCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATa                                                                                                                                      <  2:1792756/148‑1 (MQ=255)
           cGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATAc                                                                                                                                     >  2:2010985/1‑148 (MQ=255)
           cGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATAc                                                                                                                                     >  1:2683081/1‑148 (MQ=255)
              tCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGt                                                                                                                                  <  2:460064/148‑1 (MQ=255)
                ggCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCt                                                                                                                                <  2:859753/148‑1 (MQ=255)
                 gCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                               <  2:3032235/148‑1 (MQ=255)
                 gCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                               >  2:1498831/1‑148 (MQ=255)
                 gCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                               >  1:2725925/1‑148 (MQ=255)
                    aTTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCg                                                                                                                            <  1:1113741/148‑1 (MQ=255)
                     ttttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGc                                                                                                                           >  1:604640/1‑148 (MQ=255)
                     ttttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGc                                                                                                                           <  2:3312353/148‑1 (MQ=255)
                                 tataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTa                                                                                                               >  1:2083190/1‑148 (MQ=255)
                                  ataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTaa                                                                                                              <  1:1334847/148‑1 (MQ=255)
                                  ataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTaa                                                                                                              <  2:1424382/148‑1 (MQ=255)
                                   taGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAc                                                                                                             >  1:2155828/1‑148 (MQ=255)
                                    aGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAcc                                                                                                            >  1:1918490/1‑148 (MQ=255)
                                        gtcgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                        <  2:3395666/147‑1 (MQ=255)
                                            ttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaa                                                                                                    >  1:1556419/1‑148 (MQ=255)
                                             tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaat                                                                                                   >  1:831457/1‑148 (MQ=255)
                                             tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaat                                                                                                   >  2:3042326/1‑148 (MQ=255)
                                               gCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                 <  1:737904/148‑1 (MQ=255)
                                               gCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                 <  2:1887228/148‑1 (MQ=255)
                                                cTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATc                                                                                                >  2:1087794/1‑148 (MQ=255)
                                                cTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATc                                                                                                >  1:503475/1‑148 (MQ=255)
                                                 tttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCg                                                                                               <  1:2808/148‑1 (MQ=255)
                                                 tttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCg                                                                                               <  2:1176136/148‑1 (MQ=255)
                                                    cAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCg                                                                                            >  2:232167/1‑148 (MQ=255)
                                                     aGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGa                                                                                           >  1:3127342/1‑148 (MQ=255)
                                                     aGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGa                                                                                           <  2:2975802/148‑1 (MQ=255)
                                                     aGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGa                                                                                           >  1:2440628/1‑148 (MQ=255)
                                                       aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                         >  1:2832193/1‑148 (MQ=255)
                                                         cGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACggg                                                                                       <  2:3125664/148‑1 (MQ=255)
                                                          gAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGc                                                                                      >  2:2782528/1‑148 (MQ=255)
                                                            aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                    >  2:1610855/1‑148 (MQ=255)
                                                            aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                    >  1:1425893/1‑148 (MQ=255)
                                                            aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                    >  2:3276488/1‑148 (MQ=255)
                                                            aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                    >  1:2542649/1‑148 (MQ=255)
                                                             gACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGt                                                                                   >  2:690032/1‑148 (MQ=255)
                                                              aCCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGtt                                                                                  >  1:1540343/1‑148 (MQ=255)
                                                                 tGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCAt                                                                               >  1:1099609/1‑148 (MQ=255)
                                                                  gAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATg                                                                              <  2:1296198/148‑1 (MQ=255)
                                                                   aGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGc                                                                             >  2:1309104/1‑148 (MQ=255)
                                                                         aCCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGt                                                                       >  1:3516076/1‑148 (MQ=255)
                                                                          ccGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTa                                                                      <  2:2575873/148‑1 (MQ=255)
                                                                           cGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTCCATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAc                                                                     >  1:3034571/1‑148 (MQ=255)
                                                                           cGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAc                                                                     >  2:2990608/1‑148 (MQ=255)
                                                                           cGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAc                                                                     >  1:3387512/1‑148 (MQ=255)
                                                                                ttCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATg                                                                >  2:3360183/1‑148 (MQ=255)
                                                                                 tCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATgg                                                               <  1:2760685/148‑1 (MQ=255)
                                                                                 tCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATgg                                                               <  2:2393858/148‑1 (MQ=255)
                                                                                     cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAcc                                                           >  2:1656869/1‑148 (MQ=255)
                                                                                      gcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                          >  2:1161721/1‑148 (MQ=255)
                                                                                       cAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                         >  1:2326902/1‑148 (MQ=255)
                                                                                        aaTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGt                                                        <  2:1794263/148‑1 (MQ=255)
                                                                                         aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGtt                                                       <  1:990839/148‑1 (MQ=255)
                                                                                         aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAGACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGtt                                                       <  1:3421110/148‑1 (MQ=255)
                                                                                            ttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGc                                                    <  1:2952958/148‑1 (MQ=255)
                                                                                                  ggTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCtt                                              <  1:3305459/148‑1 (MQ=255)
                                                                                                   gTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTc                                             >  2:165652/1‑148 (MQ=255)
                                                                                                        cTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTcggcgg                                        >  1:526177/1‑148 (MQ=255)
                                                                                                           gCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGc                                     <  2:1918490/148‑1 (MQ=255)
                                                                                                            cAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCt                                    >  2:2758759/1‑148 (MQ=255)
                                                                                                             aaaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtc                                   <  2:1205838/148‑1 (MQ=255)
                                                                                                              aaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtca                                  <  2:1994630/148‑1 (MQ=255)
                                                                                                                gTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatc                                >  1:321198/1‑148 (MQ=255)
                                                                                                                  aTTTCTGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCTGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGTCGGTGATCATCAg                              <  2:1362873/148‑1 (MQ=255)
                                                                                                                  aTTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAg                              >  1:332808/1‑148 (MQ=255)
                                                                                                                      aTGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCaaa                          <  1:772873/148‑1 (MQ=255)
                                                                                                                       tGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAAt                         <  1:47569/148‑1 (MQ=255)
                                                                                                                        gcgcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATg                        >  1:2368099/1‑148 (MQ=255)
                                                                                                                         cgcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGa                       <  2:503475/148‑1 (MQ=255)
                                                                                                                          gcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAg                      <  1:1893864/148‑1 (MQ=255)
                                                                                                                           ccGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCACATGAgg                     >  1:3443649/1‑148 (MQ=255)
                                                                                                                           ccGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAgg                     <  1:363290/148‑1 (MQ=255)
                                                                                                                           ccGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAgg                     >  1:1187457/1‑148 (MQ=255)
                                                                                                                               aCCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAcc                 >  1:2933103/1‑148 (MQ=255)
                                                                                                                               aCCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAcc                 <  2:2978049/148‑1 (MQ=255)
                                                                                                                                 cGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCtt               >  2:3231276/1‑148 (MQ=255)
                                                                                                                                  gTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGTGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTc              <  1:383457/148‑1 (MQ=255)
                                                                                                                                  gTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGTGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTc              <  1:2191967/148‑1 (MQ=255)
                                                                                                                                  gTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTc              <  2:2083190/148‑1 (MQ=255)
                                                                                                                                  gTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTc              <  2:1981903/148‑1 (MQ=255)
                                                                                                                                    tAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAc            >  2:1010472/1‑148 (MQ=255)
                                                                                                                                     aGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACg           <  2:2898884/148‑1 (MQ=255)
                                                                                                                                      gggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGa          <  1:1837697/148‑1 (MQ=255)
                                                                                                                                       ggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt         >  1:2915571/1‑148 (MQ=255)
                                                                                                                                       ggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt         >  1:861583/1‑148 (MQ=255)
                                                                                                                                        gCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATa        <  1:1497804/148‑1 (MQ=255)
                                                                                                                                          ggTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATAcc      <  1:1679483/148‑1 (MQ=255)
                                                                                                                                            ttAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGt    >  2:2592192/1‑148 (MQ=255)
                                                                                                                                             tAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTc   <  2:1244539/148‑1 (MQ=255)
                                                                                                                                             tAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTc   <  2:407622/148‑1 (MQ=255)
                                                                                                                                              aGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCa  <  1:3042326/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCA  >  CP009273/661963‑662252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑