Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 680,846 G→T 100% F180L (TTC→TTA)  gltK ← glutamate, aspartate ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273680,8460GT98.4% 620.1 / 0.7 193F180L (TTC→TTA) gltKglutamate, aspartate ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (121/69);  total (123/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC  >  CP009273/680701‑680990
                                                                                                                                                 |                                                                                                                                                
aGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGgaataa                                                                                                                                                <  2:2281803/148‑1 (MQ=255)
aGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGgaataa                                                                                                                                                <  2:2631153/148‑1 (MQ=255)
  ggTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAAt                                                                                                                                              >  1:3189150/1‑148 (MQ=255)
     aaTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCgg                                                                                                                                           >  2:1615192/1‑148 (MQ=255)
      aTCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGc                                                                                                                                          <  1:2481128/148‑1 (MQ=255)
       tCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGcc                                                                                                                                         >  2:333428/1‑148 (MQ=255)
        cATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCa                                                                                                                                        <  1:428478/148‑1 (MQ=255)
        cATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCa                                                                                                                                        >  1:186745/1‑148 (MQ=255)
           tATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAggg                                                                                                                                     >  1:2664691/1‑148 (MQ=255)
           tATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAggg                                                                                                                                     <  2:2545550/148‑1 (MQ=255)
               cTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGACTAAATCGGCCAGGGATaa                                                                                                                                 >  2:942715/1‑148 (MQ=255)
               cTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATaa                                                                                                                                 <  1:824763/148‑1 (MQ=255)
               cTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATaa                                                                                                                                 >  1:987265/1‑148 (MQ=255)
               cTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATaa                                                                                                                                 <  2:2188133/148‑1 (MQ=255)
                 gTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAaca                                                                                                                               >  2:250987/1‑148 (MQ=255)
                      tcttTTCAAGTAGCGGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATac                                                                                                                          <  2:2394568/148‑1 (MQ=255)
                       cttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATaca                                                                                                                         <  1:574711/148‑1 (MQ=255)
                       cttTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATaca                                                                                                                         >  1:3052192/1‑148 (MQ=255)
                         tttCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACAcc                                                                                                                       >  1:595917/1‑148 (MQ=255)
                          ttCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCa                                                                                                                      <  2:1263300/148‑1 (MQ=255)
                          ttCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCa                                                                                                                      >  2:2056729/1‑148 (MQ=255)
                             atgTACCTTACAAAAAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTg                                                                                                                   <  1:3084169/146‑1 (MQ=255)
                              aGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGa                                                                                                                  >  1:2291218/1‑148 (MQ=255)
                                tAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAgg                                                                                                                <  1:1065612/148‑1 (MQ=255)
                                tAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAgg                                                                                                                >  1:1365325/1‑148 (MQ=255)
                                    tGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATc                                                                                                            <  1:2699582/148‑1 (MQ=255)
                                      aCCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCt                                                                                                          >  1:1562522/1‑148 (MQ=255)
                                      aCCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCt                                                                                                          >  2:515959/1‑148 (MQ=255)
                                      aCCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCt                                                                                                          <  1:1003641/148‑1 (MQ=255)
                                      aCCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCt                                                                                                          <  1:3299226/148‑1 (MQ=255)
                                       ccAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTg                                                                                                         >  2:1470612/1‑148 (MQ=255)
                                          acaacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGaa                                                                                                      >  1:1425982/1‑148 (MQ=255)
                                          acaacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGaa                                                                                                      >  2:2414580/1‑148 (MQ=255)
                                           caacGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAAc                                                                                                     >  1:1720066/1‑148 (MQ=255)
                                                 cgcgCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACg                                                                                               <  1:2428364/148‑1 (MQ=255)
                                                 cgcgCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACg                                                                                               >  1:822343/1‑148 (MQ=255)
                                                      tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                          <  2:1051656/148‑1 (MQ=255)
                                                      tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                          <  2:773586/148‑1 (MQ=255)
                                                      tAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGcc                                                                                          <  2:1179599/148‑1 (MQ=255)
                                                       aaGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGccc                                                                                         >  2:3007612/1‑148 (MQ=255)
                                                        aGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCt                                                                                        >  1:2103047/1‑148 (MQ=255)
                                                          aCTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAACTCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtga                                                                                      <  2:2752454/148‑1 (MQ=255)
                                                          aCTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtga                                                                                      >  1:469232/1‑148 (MQ=255)
                                                          aCTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtga                                                                                      >  2:681418/1‑148 (MQ=255)
                                                          aCTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCCGCAAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGACCtga                                                                                      >  2:2460345/1‑148 (MQ=255)
                                                           cTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgag                                                                                     >  2:937177/1‑148 (MQ=255)
                                                            taataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagt                                                                                    <  2:458388/148‑1 (MQ=255)
                                                            taataaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGAACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagt                                                                                    <  1:1218008/148‑1 (MQ=255)
                                                               taaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtgag                                                                                 <  2:2949870/148‑1 (MQ=255)
                                                               taaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtgag                                                                                 <  1:2189444/148‑1 (MQ=255)
                                                               taaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtgag                                                                                 >  2:2666350/1‑148 (MQ=255)
                                                               taaCGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgagtgag                                                                                 >  2:1733976/1‑148 (MQ=255)
                                                                    aaaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagca                                                                            >  2:699028/1‑148 (MQ=255)
                                                                     aaTAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGagcagcag                                                                           >  1:2862950/1‑148 (MQ=255)
                                                                       tAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCg                                                                         >  2:886239/1‑148 (MQ=255)
                                                                            aaaTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCAcc                                                                    >  2:3201232/1‑148 (MQ=255)
                                                                              aTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                  >  2:409957/1‑148 (MQ=255)
                                                                              aTCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCAt                                                                  >  1:2629302/1‑148 (MQ=255)
                                                                                ccGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATcg                                                                <  2:822343/148‑1 (MQ=255)
                                                                                ccGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATcg                                                                <  2:885129/148‑1 (MQ=255)
                                                                                     aaaCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCgg                                                           <  1:2874976/148‑1 (MQ=255)
                                                                                      aaCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGa                                                          <  2:3237773/148‑1 (MQ=255)
                                                                                       aCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                         >  2:1505600/1‑148 (MQ=255)
                                                                                       aCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                         >  1:1494151/1‑148 (MQ=255)
                                                                                       aCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                         >  1:908732/1‑148 (MQ=255)
                                                                                       aCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                         >  1:443073/1‑148 (MQ=255)
                                                                                       aCAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGaa                                                         >  2:3272573/1‑148 (MQ=255)
                                                                                         aGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACg                                                       <  2:962889/148‑1 (MQ=255)
                                                                                          ggATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGc                                                      <  2:2092284/148‑1 (MQ=255)
                                                                                           gATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGcc                                                     >  2:2323988/1‑148 (MQ=255)
                                                                                            atcatcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCt                                                    >  2:1850324/1‑148 (MQ=255)
                                                                                              catcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                  <  1:2653410/148‑1 (MQ=255)
                                                                                              catcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGc                                                  >  1:3128201/1‑148 (MQ=255)
                                                                                               atcTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCg                                                 >  1:1676359/1‑148 (MQ=255)
                                                                                                 ctcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCtgag                                                                                     <  1:2239667/110‑1 (MQ=255)
                                                                                                 ctcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGc                                               >  2:611523/1‑148 (MQ=255)
                                                                                                 ctcAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGc                                               >  1:3229674/1‑148 (MQ=255)
                                                                                                      ccTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaat                                          >  2:75313/1‑148 (MQ=255)
                                                                                                         gCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGaataat                                       >  1:1985024/1‑148 (MQ=255)
                                                                                                             aCCATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGt                                   >  1:1201874/1‑148 (MQ=255)
                                                                                                               cATCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGttt                                 >  1:3473761/1‑148 (MQ=255)
                                                                                                                aTCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTc                                <  1:3442203/148‑1 (MQ=255)
                                                                                                                 tCACGCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCa                               <  2:187178/148‑1 (MQ=255)
                                                                                                                     gCTCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGa                           >  1:1957389/1‑148 (MQ=255)
                                                                                                                       tCACCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACt                         >  1:2870299/1‑148 (MQ=255)
                                                                                                                         aCCAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGc                       >  1:591416/1‑148 (MQ=255)
                                                                                                                          ccAATGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGcc                      >  2:2947107/1‑148 (MQ=255)
                                                                                                                            aaTGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAg                    <  1:937430/148‑1 (MQ=255)
                                                                                                                              tGGTTGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTg                  >  2:2960414/1‑148 (MQ=255)
                                                                                                                                 ttGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGt               >  2:343634/1‑148 (MQ=255)
                                                                                                                                 ttGAGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGt               <  1:1202333/148‑1 (MQ=255)
                                                                                                                                    aGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCAt            <  2:595917/148‑1 (MQ=255)
                                                                                                                                    aGGCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCAt            >  1:2988436/1‑148 (MQ=255)
                                                                                                                                      gCGGTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTc          >  1:202986/1‑148 (MQ=255)
                                                                                                                                        ggTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTccc        <  1:1615192/148‑1 (MQ=255)
                                                                                                                                         gTACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCa       <  2:2730449/148‑1 (MQ=255)
                                                                                                                                          tACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCaa      >  1:2785485/1‑148 (MQ=255)
                                                                                                                                          tACGGAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCaa      >  1:181730/1‑148 (MQ=255)
                                                                                                                                             ggAATAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGc   >  1:3518736/1‑148 (MQ=255)
                                                                                                                                              gaataaATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGcc  >  2:1925116/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGGGTAATCATTATGCTGTCCTTCTTTTCAAGTAGCTGACCAACAACGACGCGCTAAGACTAATAACGAAATAAACAAATCCGGCAAACAGGATCATCTCAACCTGCGTACCATCACGCTCACCAATGGTTGAGGCGGTACGGAAGAAATCGGCCAGGGATAACACATACACCAGTGAGGTATCCTGGAACAGTACGATGCCCTGAGTGAGCAGCAGCGGCACCATCGCGCGGAACGCCTGCGGCAGAATAATCAGTTTCATCGACTGCCAGTGAGTCATTCCCAACGCC  >  CP009273/680701‑680990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑