Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 723,859 G→T 100% V110V (GTC→GTA)  kdpA ← potassium translocating ATPase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273723,8590GT96.4% 594.2 / 10.4 194V110V (GTC→GTA) kdpApotassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (99/88);  total (102/92)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 194 total aligned reads displayed.

CTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAG  >  CP009273/723715‑724002
                                                                                                                                                |                                                                                                                                               
ctGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACa                                                                                                                                              >  1:2850668/1‑148 (MQ=255)
 tGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACaa                                                                                                                                             <  1:2139035/148‑1 (MQ=255)
    cGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGc                                                                                                                                          <  2:2744038/148‑1 (MQ=255)
    cGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGc                                                                                                                                          <  2:1647288/148‑1 (MQ=255)
       gTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGa                                                                                                                                       <  2:1709267/148‑1 (MQ=255)
         aaaCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcg                                                                                                                                     >  1:2144406/1‑148 (MQ=255)
         aaaCGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCCGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTgggg                                                                                                                                     >  2:3386825/1‑145 (MQ=255)
             gCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGAcggcgg                                                                                                                                 >  2:3499208/1‑148 (MQ=255)
                  gATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTc                                                                                                                            <  2:123830/148‑1 (MQ=255)
                    tGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTCTTCAg                                                                                                                          >  2:2067544/1‑148 (MQ=255)
                     gAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAgc                                                                                                                         >  2:2850684/1‑148 (MQ=255)
                       gggCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAgcgc                                                                                                                       >  2:1497069/1‑148 (MQ=255)
                       gggCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAgcgc                                                                                                                       <  1:55791/148‑1 (MQ=255)
                        ggCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGcc                                                                                                                      >  1:1007654/1‑148 (MQ=255)
                         gCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCa                                                                                                                     >  1:3381392/1‑148 (MQ=255)
                         gCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCa                                                                                                                     >  1:453711/1‑148 (MQ=255)
                          cAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAg                                                                                                                    >  2:2935200/1‑148 (MQ=255)
                          cAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAg                                                                                                                    >  1:607285/1‑148 (MQ=255)
                            aaaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGAt                                                                                                                  >  2:2254699/1‑148 (MQ=255)
                             aaaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATc                                                                                                                 >  2:1972452/1‑148 (MQ=255)
                              aaTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATcc                                                                                                                >  2:2859931/1‑148 (MQ=255)
                               aTCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATccc                                                                                                               >  1:1721827/1‑148 (MQ=255)
                                tCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTCTAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATGCAGCGCCAGATCCCa                                                                                                              >  2:3218216/1‑148 (MQ=255)
                                tCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCa                                                                                                              <  2:2359744/148‑1 (MQ=255)
                                  aCCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACg                                                                                                            >  2:3411605/1‑148 (MQ=255)
                                      cAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAg                                                                                                        >  1:1261129/1‑148 (MQ=255)
                                        aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGcc                                                                                                      <  1:1879445/148‑1 (MQ=255)
                                        aTCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGcc                                                                                                      <  2:259369/148‑1 (MQ=255)
                                          cccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCt                                                                                                    <  1:1249966/148‑1 (MQ=255)
                                          cccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCt                                                                                                    >  2:1814431/1‑148 (MQ=255)
                                          cccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCt                                                                                                    <  1:1248960/148‑1 (MQ=255)
                                          cccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCt                                                                                                    <  1:3025320/148‑1 (MQ=255)
                                          cccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCt                                                                                                    >  1:2497469/1‑148 (MQ=255)
                                           ccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTg                                                                                                   <  1:3403647/148‑1 (MQ=255)
                                           ccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTg                                                                                                   >  1:878446/1‑148 (MQ=255)
                                           ccGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTg                                                                                                   >  2:2341786/1‑148 (MQ=255)
                                                    gcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgct                                                                                          >  1:2387267/1‑148 (MQ=255)
                                                    gcAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgct                                                                                          <  2:1007654/148‑1 (MQ=255)
                                                     cAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAActgctg                                                                                         <  1:684751/148‑1 (MQ=255)
                                                       gaaagaaaGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg                                                                                       <  1:2746846/148‑1 (MQ=255)
                                                       gaaagaaaGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCtgtg                                                                                       >  1:2210744/1‑148 (MQ=255)
                                                        aaagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTgg                                                                                      <  1:3523918/148‑1 (MQ=255)
                                                         aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATCAGATTGACAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGa                                                                                     <  1:1966508/148‑1 (MQ=255)
                                                         aagaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGCGGa                                                                                     <  2:1397263/148‑1 (MQ=255)
                                                          agaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAt                                                                                    <  2:673179/148‑1 (MQ=255)
                                                          agaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAt                                                                                    >  1:765025/1‑148 (MQ=255)
                                                           gaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAtt                                                                                   >  1:2329522/1‑148 (MQ=255)
                                                           gaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGAtt                                                                                   <  2:3002407/148‑1 (MQ=255)
                                                           gaaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACCGCTGTGGAtt                                                                                   <  2:157672/148‑1 (MQ=255)
                                                            aaagTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTa                                                                                  <  2:39919/148‑1 (MQ=255)
                                                                  ttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGc                                                                            <  2:220881/148‑1 (MQ=255)
                                                                  ttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGc                                                                            <  2:1961994/148‑1 (MQ=255)
                                                                  ttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGc                                                                            <  1:1972792/148‑1 (MQ=255)
                                                                  ttGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGc                                                                            <  1:3221988/148‑1 (MQ=255)
                                                                     cACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGa                                                                         <  2:336766/148‑1 (MQ=255)
                                                                       ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                       <  2:2885749/148‑1 (MQ=255)
                                                                       ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                       <  2:2429002/148‑1 (MQ=255)
                                                                       ccGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATa                                                                       <  2:1673789/148‑1 (MQ=255)
                                                                          ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCTGCAGATAGTg                                                                    >  1:3342822/1‑148 (MQ=255)
                                                                             aGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTg                                                                 <  2:1589659/148‑1 (MQ=255)
                                                                               cccGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGAc                                                               <  1:2278559/148‑1 (MQ=255)
                                                                                ccGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGAcc                                                              >  1:2546545/1‑148 (MQ=255)
                                                                                      tCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCaa                                                        >  1:3340649/1‑148 (MQ=255)
                                                                                      tCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGAATACAGCGCCAGATCCCACGACAGCACTGGCAACTGATGTGGATTAAGCGGAAGATAGTGCTGACCGAGCaa                                                        >  1:2360777/1‑148 (MQ=255)
                                                                                       cTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAAc                                                       >  2:3237512/1‑148 (MQ=255)
                                                                                       cTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAAc                                                       >  1:1866527/1‑148 (MQ=255)
                                                                                        tGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACa                                                      >  1:2926182/1‑148 (MQ=255)
                                                                                             gAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaa                                                 >  2:941810/1‑148 (MQ=255)
                                                                                             gAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGACAGTGCTGACCGAGCAACATaaaa                                                 >  1:579571/1‑148 (MQ=255)
                                                                                              aaaTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATaaaaa                                                <  2:666042/148‑1 (MQ=255)
                                                                                                aTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAAc                                              >  2:2130230/1‑148 (MQ=255)
                                                                                                 tAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACa                                             >  2:2830041/1‑148 (MQ=255)
                                                                                                 tAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACa                                             >  2:162646/1‑148 (MQ=255)
                                                                                                    cTCAACGTGGTTTCACCGCTATAAGATTGCCCGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCa                                          >  2:3104915/1‑148 (MQ=255)
                                                                                                      cAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCAcc                                        >  2:564954/1‑148 (MQ=255)
                                                                                                          gTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCa                                    <  2:133954/148‑1 (MQ=255)
                                                                                                             gTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcc                                 >  2:2390138/1‑148 (MQ=255)
                                                                                                              tttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGccc                                >  1:3465781/1‑148 (MQ=255)
                                                                                                               ttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcccc                               >  1:116066/1‑148 (MQ=255)
                                                                                                               ttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcccc                               >  2:2285948/1‑148 (MQ=255)
                                                                                                               ttCACCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGcccc                               >  2:496999/1‑148 (MQ=255)
                                                                                                                  aCCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGc                            <  1:1570508/148‑1 (MQ=255)
                                                                                                                  aCCGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGc                            >  2:3449640/1‑148 (MQ=255)
                                                                                                                   ccGCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCa                           >  1:1099187/1‑148 (MQ=255)
                                                                                                                     gCTATAAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATg                         <  1:546695/148‑1 (MQ=255)
                                                                                                                       tataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGtt                       >  2:3193267/1‑148 (MQ=255)
                                                                                                                       tataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGtt                       <  2:1124092/148‑1 (MQ=255)
                                                                                                                        ataAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTc                      <  1:1367473/148‑1 (MQ=255)
                                                                                                                         taAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCa                     >  1:3082028/1‑148 (MQ=255)
                                                                                                                         taAGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGAGGCAGATAGTGCTGACCGAGCAACATAAAAAAAAGCACAGACAGCCCCAGCATGTTCa                     >  1:3177598/1‑148 (MQ=255)
                                                                                                                          aaGATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAg                    >  2:783008/1‑148 (MQ=255)
                                                                                                                            gATTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAGCAGCACCGCCAGCCCCAGCATGTTCAGGc                  <  2:3199155/148‑1 (MQ=255)
                                                                                                                             aTTGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGcc                 >  1:484044/1‑148 (MQ=255)
                                                                                                                               tGCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCga               >  2:990738/1‑148 (MQ=255)
                                                                                                                                gCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCgag              <  2:1323894/148‑1 (MQ=255)
                                                                                                                                gCCAGTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCgag              >  1:2041895/1‑148 (MQ=255)
                                                                                                                                    gTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATg          <  1:2859931/148‑1 (MQ=255)
                                                                                                                                    gTTGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATg          >  1:1905446/1‑148 (MQ=255)
                                                                                                                                      tGGTATTGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGc        >  1:123332/1‑148 (MQ=255)
                                                                                                                                            tGGTTACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAg  <  2:2780252/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CTGGCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAG  >  CP009273/723715‑724002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑