Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 724,813 A→T 100% intergenic (+17/‑226) ybfA → / → rhsC DUF2517 family protein/Rhs family putative polymorphic toxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273724,8130AT99.5% 623.8 / ‑6.4 205intergenic (+17/‑226)ybfA/rhsCDUF2517 family protein/Rhs family putative polymorphic toxin
Reads supporting (aligned to +/- strand):  ref base A (0/4);  new base T (82/119);  total (82/123)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

TGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGA  >  CP009273/724669‑724956
                                                                                                                                                |                                                                                                                                               
 gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAtt                                                                                                                                             >  1:3482514/1‑148 (MQ=255)
 gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAtt                                                                                                                                             <  1:1332150/148‑1 (MQ=255)
 gCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCAtt                                                                                                                                             >  2:584443/1‑148 (MQ=255)
  ccTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTg                                                                                                                                            >  1:1748644/1‑148 (MQ=255)
     ttCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaa                                                                                                                                         >  2:3030820/1‑148 (MQ=255)
     ttCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGaaa                                                                                                                                         >  1:2557769/1‑148 (MQ=255)
       cATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAAc                                                                                                                                       <  2:2478202/148‑1 (MQ=255)
          gCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGc                                                                                                                                    >  2:2915189/1‑148 (MQ=255)
          gCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGATACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGc                                                                                                                                    >  2:632505/1‑148 (MQ=255)
           cTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGcc                                                                                                                                   >  2:3259189/1‑148 (MQ=255)
             tttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                 <  1:512822/148‑1 (MQ=255)
             tttCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAg                                                                                                                                 >  2:3392294/1‑148 (MQ=255)
                cTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCgg                                                                                                                              <  2:1511856/148‑1 (MQ=255)
                  ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaa                                                                                                                            <  1:780864/148‑1 (MQ=255)
                  ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaa                                                                                                                            <  1:937730/148‑1 (MQ=255)
                   gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaaa                                                                                                                           >  2:1509046/1‑148 (MQ=255)
                   gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGaaa                                                                                                                           <  2:3068639/148‑1 (MQ=255)
                      aGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGc                                                                                                                        <  1:309747/148‑1 (MQ=255)
                      aGACCGCGCCCGCTTTTACAGCTACCTGAATCGCGTCTGGTCGAAAACCATCGATAAACCGGTGTGGATGTATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGc                                                                                                                        <  1:3363125/148‑1 (MQ=255)
                       gACCGCGCCCGCATTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAACAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCt                                                                                                                       <  2:2744363/148‑1 (MQ=255)
                        aCCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTg                                                                                                                      <  2:2723595/148‑1 (MQ=255)
                           gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCg                                                                                                                   >  1:2018537/1‑148 (MQ=255)
                              cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                <  1:3460671/148‑1 (MQ=255)
                               ccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGACTTCATTGAAAACCGCCAGCGGAAACGCGGGCGGttt                                                                                                               >  2:1964761/1‑148 (MQ=255)
                                cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGtttt                                                                                                              <  2:597205/148‑1 (MQ=255)
                                cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttt                                                                                                              <  2:1711697/148‑1 (MQ=255)
                                 gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTGTTGAATTCATTGAAATCCGCCAGCGGAAACGCTGGCGGtgttt                                                                                                             >  2:47859/1‑148 (MQ=255)
                                   ttttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                           >  1:650553/1‑148 (MQ=255)
                                     ttACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTa                                                                                                         <  2:3480226/148‑1 (MQ=255)
                                          gCTACCTGCATCGCGTGTGGTCGAAAACCAGCGATATACCTTTGTGTATGTTTCAGGCCGAAAAGGCTACTTGTGATTTTTATTTTTTTCTTAGTTTTGAATTCATTGAAAACCTCCAGCGGAAACGCTGGCGGTTTTTTGTATCAgg                                                                                                    <  2:1412371/148‑1 (MQ=255)
                                           cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCATGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTAATTGAAAACCGCCATCGGAAACGCTGGCGGTTTTTTTTATAAGGa                                                                                                   >  1:169171/1‑148 (MQ=255)
                                             aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGTATGGATCAGGCCTAAAAGGAAACTGTTGATTTTTATTGATTGCTGAGTTTTGATTTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                 <  1:3448424/148‑1 (MQ=255)
                                             aCCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                 <  2:609317/148‑1 (MQ=255)
                                              ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGa                                                                                                <  1:1398080/148‑1 (MQ=255)
                                                 gCATCGCGTCTGGTCTAAAACCAGCTATAAACCGGTGTGTATGGATCCGGCCGAAAAGGCAAGTGGTGATTTTTATTGATTGCTGAGTTTTTAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                             <  1:717901/148‑1 (MQ=255)
                                                 gCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                             <  2:3226555/148‑1 (MQ=255)
                                                    tCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCa                                                                                          >  1:1109649/1‑148 (MQ=255)
                                                      gcgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCaga                                                                                        <  2:2962698/148‑1 (MQ=255)
                                                       cgTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCagag                                                                                       <  1:2331654/148‑1 (MQ=255)
                                                          cTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGaa                                                                                    <  2:3150067/148‑1 (MQ=255)
                                                             gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAatat                                                                                 <  1:2743481/148‑1 (MQ=255)
                                                              tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                >  2:1832942/1‑148 (MQ=255)
                                                               cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                               >  1:207748/1‑148 (MQ=255)
                                                                 aaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                             <  1:515501/148‑1 (MQ=255)
                                                                 aaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                             >  2:2361371/1‑148 (MQ=255)
                                                                 aaaaCCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                             <  2:210758/148‑1 (MQ=255)
                                                                       aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCg                                                                       <  1:1949444/148‑1 (MQ=255)
                                                                       aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCg                                                                       <  2:3233362/148‑1 (MQ=255)
                                                                        gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                      <  2:1227314/148‑1 (MQ=255)
                                                                        gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                      <  1:427329/148‑1 (MQ=255)
                                                                           aTAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtat                                                                   <  2:1839639/148‑1 (MQ=255)
                                                                            tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtata                                                                  >  2:875489/1‑148 (MQ=255)
                                                                            tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtata                                                                  <  2:2661083/148‑1 (MQ=255)
                                                                             aaaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGATGTTAAGAGGAATATTTAGACATAGTtataa                                                                 >  1:2443143/1‑147 (MQ=255)
                                                                              aaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                >  2:1129186/1‑148 (MQ=255)
                                                                                ccGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCaa                                                              <  2:2627080/148‑1 (MQ=255)
                                                                                 cGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAAt                                                             <  1:2915189/148‑1 (MQ=255)
                                                                                      tggatggaTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaaa                                                        <  1:1233789/148‑1 (MQ=255)
                                                                                       ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAc                                                       <  1:103451/148‑1 (MQ=255)
                                                                                         atggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                     >  1:1450960/1‑148 (MQ=255)
                                                                                           ggatCAGGCCGAAACGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatat                                                   <  1:983217/148‑1 (MQ=255)
                                                                                           ggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatat                                                   <  2:2297228/148‑1 (MQ=255)
                                                                                            gatCAGGCCGAAAAGTCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTTAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatata                                                  <  2:2048355/148‑1 (MQ=255)
                                                                                            gatCAGGCCGAAAAGGCAACTGTTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatata                                                  <  1:538084/148‑1 (MQ=255)
                                                                                            gatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatata                                                  <  2:1189154/148‑1 (MQ=255)
                                                                                            gatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatata                                                  <  2:3302448/148‑1 (MQ=255)
                                                                                             atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                 <  2:1177731/148‑1 (MQ=255)
                                                                                             atCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACatatat                                                 >  2:358518/1‑148 (MQ=255)
                                                                                              tCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATAtt                                                <  1:96620/148‑1 (MQ=255)
                                                                                               cAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTc                                               <  2:739042/148‑1 (MQ=255)
                                                                                                 ggCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTAAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTAAt                                             >  2:727894/1‑148 (MQ=255)
                                                                                                    cGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaa                                          <  2:1344577/148‑1 (MQ=255)
                                                                                                        aaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatat                                      >  2:167524/1‑148 (MQ=255)
                                                                                                          ggCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatat                                    <  1:2700755/148‑1 (MQ=255)
                                                                                                          ggCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatat                                    >  2:861230/1‑148 (MQ=255)
                                                                                                           gCAACTTGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatata                                   <  2:1887526/148‑1 (MQ=255)
                                                                                                           gCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatata                                   <  2:2002116/148‑1 (MQ=255)
                                                                                                             aaCTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAAtatatata                                 >  2:2736833/1‑148 (MQ=255)
                                                                                                               cTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATaaa                               >  2:2957242/1‑148 (MQ=255)
                                                                                                               cTGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTTATACCAATCGAAACATATATTCATGAAATATATATaaa                               >  2:1246560/1‑148 (MQ=255)
                                                                                                                tGGTGATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATCAGGAAGAATTTCAGAGGAAAATTTATACAAAGTTATACCAATCCAAACAAAAAATAAATAAAAATATATaaaa                              >  1:1048930/1‑147 (MQ=255)
                                                                                                                   tGATTTTTATGGCTTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAtt                           <  1:2607537/148‑1 (MQ=255)
                                                                                                                    gATTTTTTTGGATTTCTGTGTTTTGAATTCTCTGAAAACCGCCAGCGGAAAGTCTGGGGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACAAATCGAAACATTTATTCATGAAATATATATAAATAttt                          <  1:2592433/148‑1 (MQ=255)
                                                                                                                    gATTTTTATTGTTTGCTGAGTTTTGAATACATAGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                          <  1:1849695/148‑1 (MQ=255)
                                                                                                                    gATTTTTATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATAttt                          >  2:528005/1‑148 (MQ=255)
                                                                                                                      tttttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                        <  1:3259189/148‑1 (MQ=255)
                                                                                                                       ttttATTGATTGCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTcc                       <  1:873462/148‑1 (MQ=255)
                                                                                                                           attgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATTAAATATATATAAAAATTTTaataat                   >  2:2451143/1‑148 (MQ=255)
                                                                                                                           attgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAt                   <  1:3255303/148‑1 (MQ=255)
                                                                                                                            ttgattgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAATATTTTCAGTGGAAAAATTATACAAAGTTATAAAAATAGAAAAAAAAAATAAATAAATAAATATAAAAAttttaaaaaat                  >  1:410650/1‑141 (MQ=255)
                                                                                                                               attgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGtt               <  2:1126692/148‑1 (MQ=255)
                                                                                                                                ttgCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGtta              >  2:2832629/1‑147 (MQ=255)
                                                                                                                                  gCTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCtt            <  1:2962605/148‑1 (MQ=255)
                                                                                                                                   cTGAGTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTa           >  2:1445005/1‑148 (MQ=255)
                                                                                                                                       gTTTTGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGTAGATGTTAAGAGGAATATTTTGAAATAGTTATAACCAACGAAACAAATATTCATGAAATATATATAAATATTTTACTAATTGTTATTATATg       >  2:1209044/1‑148 (MQ=255)
                                                                                                                                        ttttGAATTCATTCAAAACCGCCAGCTGAATCGGTGGCGGTTTTTTGTATCATGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTAATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGa      <  1:903660/148‑1 (MQ=255)
                                                                                                                                           tGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg   <  2:1109649/148‑1 (MQ=255)
                                                                                                                                           tGAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg   <  2:160457/148‑1 (MQ=255)
                                                                                                                                           tGAATTAATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg   <  1:584493/148‑1 (MQ=255)
                                                                                                                                            gAATTCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTCATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGa  <  1:3391407/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TGCCTTTCATGCTTTTCTGGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGA  >  CP009273/724669‑724956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑