Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 748,707 A→T 92.8% I406I (ATT→ATA)  gltA ← citrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273748,7070AT92.8% 618.6 / 28.6 202I406I (ATT→ATA) gltAcitrate synthase
Reads supporting (aligned to +/- strand):  ref base A (5/10);  new base T (102/85);  total (107/95)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

TTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAC  >  CP009273/748562‑748851
                                                                                                                                                 |                                                                                                                                                
ttttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTAt                                                                                                                                                >  1:2576532/1‑148 (MQ=255)
 tttttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATc                                                                                                                                               >  1:1264531/1‑148 (MQ=255)
    ttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTc                                                                                                                                            <  2:432038/148‑1 (MQ=255)
    ttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTc                                                                                                                                            >  2:2713131/1‑148 (MQ=255)
    ttGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTc                                                                                                                                            >  1:956575/1‑148 (MQ=255)
          ttAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCg                                                                                                                                      >  1:2863788/1‑148 (MQ=255)
            aGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTc                                                                                                                                    <  1:2393850/148‑1 (MQ=255)
            aGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTc                                                                                                                                    <  1:2393907/148‑1 (MQ=255)
             ggCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCa                                                                                                                                   >  2:2747182/1‑148 (MQ=255)
                atatAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtg                                                                                                                                >  2:1430689/1‑148 (MQ=255)
                 tataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACtgt                                                                                                                               >  1:1751118/1‑148 (MQ=255)
                      aaaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCAtt                                                                                                                          >  2:1809977/1‑148 (MQ=255)
                       aaTCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCAttt                                                                                                                         >  1:3155317/1‑148 (MQ=255)
                         tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCg                                                                                                                       >  2:1827443/1‑148 (MQ=255)
                         tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCg                                                                                                                       >  1:317038/1‑148 (MQ=255)
                         tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCg                                                                                                                       >  2:1257659/1‑148 (MQ=255)
                         tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCg                                                                                                                       <  1:3341427/148‑1 (MQ=255)
                         tCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCg                                                                                                                       <  1:1032931/148‑1 (MQ=255)
                          cAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGc                                                                                                                      >  1:2306671/1‑148 (MQ=255)
                                gCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGt                                                                                                                >  1:2034387/1‑148 (MQ=255)
                                   atatGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTggg                                                                                                             >  2:405473/1‑148 (MQ=255)
                                     atGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCg                                                                                                           >  2:799997/1‑148 (MQ=255)
                                            gcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGc                                                                                                    <  1:2641216/148‑1 (MQ=255)
                                            gcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGc                                                                                                    <  2:2580492/148‑1 (MQ=255)
                                            gcggGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGc                                                                                                    <  1:1854918/148‑1 (MQ=255)
                                              gggTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCa                                                                                                  <  1:1370486/148‑1 (MQ=255)
                                                 ttAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACg                                                                                               >  2:3119656/1‑148 (MQ=255)
                                                 ttAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACg                                                                                               >  2:1547478/1‑148 (MQ=255)
                                                  tAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACgg                                                                                              <  1:2585647/148‑1 (MQ=255)
                                                   aaaaTATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGt                                                                                             >  1:2063441/1‑148 (MQ=255)
                                                      atatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTAGCTCCAGTGGGCGATCCAGCCAACGGTACg                                                                                          >  1:1689138/1‑148 (MQ=255)
                                                      atatTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATACTGTATACAGCTGACGCGGACGGGCAATCTAAATACCGTCACAGTGCAATTAGCTACAGTGGGCGATCCAGCCAACGGTAcc                                                                                          >  1:1687940/1‑147 (MQ=255)
                                                          ttACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGcc                                                                                      >  2:3298126/1‑148 (MQ=255)
                                                           tACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCa                                                                                     >  2:2950903/1‑148 (MQ=255)
                                                            aCAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAt                                                                                    <  2:3252694/148‑1 (MQ=255)
                                                            aCAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCAt                                                                                    >  1:1416315/1‑148 (MQ=255)
                                                              aaCTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTg                                                                                  >  1:705686/1‑148 (MQ=255)
                                                               aCTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGc                                                                                 >  1:151998/1‑148 (MQ=255)
                                                                cTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCCTTGCg                                                                                >  1:2891565/1‑148 (MQ=255)
                                                                cTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCg                                                                                >  1:2365081/1‑148 (MQ=255)
                                                                  tAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCAGTATACAGCAGACCCCGACGGGCTATCTTCATAACCTCACTGTGAAATTCGCTCCAGTGGGCGATCCAGCCACAGGTACGTGCCCTTGGGaa                                                                              >  1:948871/1‑148 (MQ=255)
                                                                   aGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGaaa                                                                             <  2:2863788/148‑1 (MQ=255)
                                                                      aaTCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATg                                                                          <  2:1165860/148‑1 (MQ=255)
                                                                         cAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACg                                                                       >  2:3299678/1‑148 (MQ=255)
                                                                            ccATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTg                                                                    >  2:1039258/1‑148 (MQ=255)
                                                                             cATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGa                                                                   >  1:3539037/1‑148 (MQ=255)
                                                                               ttAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCACCGGTACGTGCCATTGCGAAAATGACGGTGAAc                                                                 >  2:620021/1‑148 (MQ=255)
                                                                                tAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACa                                                                <  1:1302287/148‑1 (MQ=255)
                                                                                tAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACa                                                                >  1:2952575/1‑148 (MQ=255)
                                                                                tAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGAACCAGCCAACGGTACGTGCCCTTGCGAAAATGACGGTGAACa                                                                >  2:2131790/1‑148 (MQ=255)
                                                                                 aaCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACAt                                                               <  2:360453/148‑1 (MQ=255)
                                                                                  aCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATg                                                              >  2:1241447/1‑148 (MQ=255)
                                                                                  aCGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATg                                                              >  2:1597867/1‑148 (MQ=255)
                                                                                       tGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                         >  2:947611/1‑148 (MQ=255)
                                                                                       tGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                         <  2:2558743/148‑1 (MQ=255)
                                                                                       tGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                         <  1:1579379/148‑1 (MQ=255)
                                                                                       tGATATCGCTTTTAAAGTCGCGATTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGa                                                         <  1:110303/148‑1 (MQ=255)
                                                                                         atatCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACg                                                       >  2:366765/1‑148 (MQ=255)
                                                                                           atCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGa                                                     >  1:2206643/1‑148 (MQ=255)
                                                                                             cGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAAt                                                   <  1:3052469/148‑1 (MQ=255)
                                                                                              gCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATa                                                  <  1:1026934/148‑1 (MQ=255)
                                                                                              gCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATa                                                  <  1:1043237/148‑1 (MQ=255)
                                                                                               cTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAc                                                 <  2:194621/148‑1 (MQ=255)
                                                                                                ttttAAAGTCGCGTTTGTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATAcc                                                <  2:1087937/148‑1 (MQ=255)
                                                                                                       gTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCt                                         <  1:489565/148‑1 (MQ=255)
                                                                                                        tCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCtt                                        <  1:1898843/148‑1 (MQ=255)
                                                                                                         cgcgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCttt                                       >  1:1174509/1‑148 (MQ=255)
                                                                                                         cgcgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCttt                                       <  2:3024584/148‑1 (MQ=255)
                                                                                                           cgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCTCTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCa                                     <  1:910781/148‑1 (MQ=255)
                                                                                                           cgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCa                                     >  1:838701/1‑148 (MQ=255)
                                                                                                           cgTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCa                                     <  2:84655/148‑1 (MQ=255)
                                                                                                             tttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAgg                                   >  2:2256364/1‑148 (MQ=255)
                                                                                                             tttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAgg                                   <  2:838024/148‑1 (MQ=255)
                                                                                                              ttttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGga                                  <  2:317038/148‑1 (MQ=255)
                                                                                                                ttCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatg                                <  2:1006298/148‑1 (MQ=255)
                                                                                                                 tCATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatga                               <  2:952398/148‑1 (MQ=255)
                                                                                                                  cATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatgat                              <  1:2468795/148‑1 (MQ=255)
                                                                                                                  cATATCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGgatgat                              <  2:3242244/148‑1 (MQ=255)
                                                                                                                    tatCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAc                            <  2:3155317/148‑1 (MQ=255)
                                                                                                                     atCCTTTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAcc                           >  1:2411679/1‑148 (MQ=255)
                                                                                                                     atCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGACTACCCATCGCTTTCAGGATGATAcc                           >  2:518556/1‑148 (MQ=255)
                                                                                                                     atCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGACTACCCATCGCTTTCAGGATGATAcc                           >  2:1374666/1‑148 (MQ=255)
                                                                                                                     atCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAcc                           <  1:2727313/148‑1 (MQ=255)
                                                                                                                     atCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAcc                           >  2:2170848/1‑148 (MQ=255)
                                                                                                                     atCCTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATAcc                           >  2:1636083/1‑148 (MQ=255)
                                                                                                                       ccTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCag                         <  2:34311/148‑1 (MQ=255)
                                                                                                                        cTGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCaga                        >  1:3180476/1‑148 (MQ=255)
                                                                                                                         tGTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCagag                       >  1:2961972/1‑148 (MQ=255)
                                                                                                                          gTATACAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGt                      >  1:1470831/1‑148 (MQ=255)
                                                                                                                           tataCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTa                     >  1:1223642/1‑148 (MQ=255)
                                                                                                                             taCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGa                   >  2:3504679/1‑148 (MQ=255)
                                                                                                                              aCAGCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGaa                  <  2:674157/148‑1 (MQ=255)
                                                                                                                                 gCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc               <  2:643439/148‑1 (MQ=255)
                                                                                                                                 gCTGACGCGGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATc               <  2:710260/148‑1 (MQ=255)
                                                                                                                                      cgcgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGt          <  2:311161/148‑1 (MQ=255)
                                                                                                                                       gcgGACGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGtt         <  2:2306161/148‑1 (MQ=255)
                                                                                                                                           aCGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCggg     >  2:1796611/1‑148 (MQ=255)
                                                                                                                                           aCGGGCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCggg     <  2:252031/148‑1 (MQ=255)
                                                                                                                                              ggCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGACTACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAc  >  2:1457576/1‑148 (MQ=255)
                                                                                                                                              ggCTATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAc  <  2:3541200/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCGTCACTGTGCATTTCGCTCCAGTGGGCGATCCAGCCAACGGTACGTGCCATTGCGAAAATGACGGTGAACATGGAAGACGGAATACCCATCGCTTTCAGGATGATACCAGAGTAGAAATCGACGTTCGGGTAC  >  CP009273/748562‑748851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑