Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 805,678 G→T 30.4% T293T (ACG→ACT)  bioB → biotin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273805,6780GT30.4% 332.8 / 130.1 178T293T (ACG→ACT) bioBbiotin synthase
Reads supporting (aligned to +/- strand):  ref base G (60/64);  new base T (24/30);  total (84/94)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 181 total aligned reads displayed.

TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA  >  CP009273/805531‑805821
                                                                                                                                                   |                                                                                                                                               
tCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACg                                                                                                                                                 >  2:2472211/1‑148 (MQ=255)
tCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATGTTCTACGGTTGCAAACTGCTGACCACg                                                                                                                                                 >  2:2430739/1‑148 (MQ=255)
    aCCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGa                                                                                                                                             <  1:3227415/148‑1 (MQ=255)
    aCCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGa                                                                                                                                             <  2:1980135/148‑1 (MQ=255)
     ccATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGaa                                                                                                                                            >  1:1613517/1‑148 (MQ=255)
     ccATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGaa                                                                                                                                            >  1:3375326/1‑148 (MQ=255)
       aTTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCATGCGATGTGCTTTATGGCAGGCGCAAAGTCGATTTTCTACGGTTGCATACTTATGACGACGCCTAATc                                                                                                                                          <  2:40030/148‑1 (MQ=255)
       aTTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATc                                                                                                                                          <  2:39060/148‑1 (MQ=255)
       aTTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATc                                                                                                                                          >  2:419038/1‑148 (MQ=255)
       aTTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATc                                                                                                                                          >  2:1532053/1‑148 (MQ=255)
        ttGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATcc                                                                                                                                         >  1:902064/1‑148 (MQ=255)
                gcgctGATCTTGATGCCAACCTCTTACGTGCGGATTTCTGCCGGACGATATCATATTAACGAACAGACTCAGGCGATGTGCTTTATGGCAGTAGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGaagaag                                                                                                                                 <  2:3342078/148‑1 (MQ=255)
                     gATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATaaa                                                                                                                            >  2:2469258/1‑148 (MQ=255)
                      aTCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGACTCCGGAAGAAGATAAAg                                                                                                                           >  2:2007539/1‑148 (MQ=255)
                       tCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGa                                                                                                                          >  1:391828/1‑148 (MQ=255)
                        ctttatgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGAACACGCCGAATCCGGAAGAAGATAAAGAc                                                                                                                         <  1:1781680/144‑1 (MQ=255)
                        cATTATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGAc                                                                                                                         <  2:2493909/148‑1 (MQ=255)
                         atgatgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGAcc                                                                                                                        <  1:2644024/148‑1 (MQ=255)
                         atgatgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGAcc                                                                                                                        <  1:863580/148‑1 (MQ=255)
                            atgCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGc                                                                                                                     >  1:2279031/1‑148 (MQ=255)
                               ccAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAAc                                                                                                                  <  2:84681/148‑1 (MQ=255)
                               ccAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAAc                                                                                                                  <  2:84659/148‑1 (MQ=255)
                                cttccTCTTACGTGCGCCTTTCTGCCGGACGCTATCTGATTAAAGAACAGACTCAGGCTATGTTCTTTATGGCAGGCGAAAACTCGATTTTCTACGGTTTCAAACTTATGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACt                                                                                                                 <  1:2430739/145‑1 (MQ=255)
                                 aaCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTg                                                                                                                >  2:1287674/1‑148 (MQ=255)
                                 aaCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTg                                                                                                                <  1:1097751/148‑1 (MQ=255)
                                  aCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGt                                                                                                               >  1:2842587/1‑148 (MQ=255)
                                  aCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGt                                                                                                               >  1:951518/1‑148 (MQ=255)
                                  aCCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGt                                                                                                               >  1:2841341/1‑148 (MQ=255)
                                   ccTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGtt                                                                                                              >  2:686576/1‑148 (MQ=255)
                                   ccTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGtt                                                                                                              <  2:595100/148‑1 (MQ=255)
                                      ctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCg                                                                                                           >  2:3452797/1‑148 (MQ=255)
                                      ctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCg                                                                                                           >  2:921480/1‑148 (MQ=255)
                                      ctTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCg                                                                                                           >  1:2433469/1‑148 (MQ=255)
                                          cGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCaaa                                                                                                       <  2:3431563/148‑1 (MQ=255)
                                          cGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCaaa                                                                                                       <  2:1032068/148‑1 (MQ=255)
                                           gTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAAc                                                                                                      <  2:1805525/148‑1 (MQ=255)
                                             gcgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTg                                                                                                    >  2:3149601/1‑148 (MQ=255)
                                             gcgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTg                                                                                                    <  1:273468/148‑1 (MQ=255)
                                             gcgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTg                                                                                                    >  2:11518/1‑148 (MQ=255)
                                              cgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTgg                                                                                                   <  2:749214/148‑1 (MQ=255)
                                              cgcCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTgg                                                                                                   >  1:3539007/1‑148 (MQ=255)
                                                ccTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTgggg                                                                                                 <  1:1510355/148‑1 (MQ=255)
                                                   ttCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTa                                                                                              >  2:2600757/1‑148 (MQ=255)
                                                    tCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCTCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTaa                                                                                             <  2:2424534/148‑1 (MQ=255)
                                                    tCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTaa                                                                                             <  2:3395667/148‑1 (MQ=255)
                                                    tCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTaa                                                                                             <  2:3396150/148‑1 (MQ=255)
                                                    tCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTaa                                                                                             <  1:1078640/148‑1 (MQ=255)
                                                     cTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTaaa                                                                                            <  2:1378925/148‑1 (MQ=255)
                                                     cTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTaaa                                                                                            >  2:1452741/1‑148 (MQ=255)
                                                      tGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAAt                                                                                           <  1:2751587/148‑1 (MQ=255)
                                                           gACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCgca                                                                                      <  2:1173536/148‑1 (MQ=255)
                                                                gAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCaaa                                                                                 <  2:1808965/148‑1 (MQ=255)
                                                                      aTGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCg                                                                           >  1:3136126/1‑148 (MQ=255)
                                                                          acgaacAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCt                                                                       >  2:1712573/1‑148 (MQ=255)
                                                                              acAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCa                                                                   <  2:1959205/148‑1 (MQ=255)
                                                                               cAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAg                                                                  <  1:2432660/148‑1 (MQ=255)
                                                                                 gACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAggg                                                                <  2:3528281/148‑1 (MQ=255)
                                                                                 gACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAggg                                                                <  2:3528596/148‑1 (MQ=255)
                                                                                  aCTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAgggg                                                               <  2:806735/148‑1 (MQ=255)
                                                                                  aCTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAgggg                                                               >  1:2993605/1‑148 (MQ=255)
                                                                                            tgtgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaaca                                                     >  1:2374017/1‑148 (MQ=255)
                                                                                            tgtgCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGaaca                                                     <  2:2432733/148‑1 (MQ=255)
                                                                                                cTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAg                                                 <  1:737598/148‑1 (MQ=255)
                                                                                                cTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAg                                                 <  1:737662/148‑1 (MQ=255)
                                                                                                 tttATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGc                                                <  1:2377450/148‑1 (MQ=255)
                                                                                                   tATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCaa                                              <  1:34368/148‑1 (MQ=255)
                                                                                                     tGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACg                                            >  2:747891/1‑148 (MQ=255)
                                                                                                       gCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTc                                          >  2:2973999/1‑148 (MQ=255)
                                                                                                       gCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGATAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTc                                          <  1:11518/148‑1 (MQ=255)
                                                                                                          ggCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTg                                       <  1:3121650/148‑1 (MQ=255)
                                                                                                           gcgcAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGa                                      >  2:1584709/1‑148 (MQ=255)
                                                                                                           gcgcAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGa                                      <  1:339781/148‑1 (MQ=255)
                                                                                                              cAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACa                                   <  2:906576/148‑1 (MQ=255)
                                                                                                                aaCTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAgg                                 <  2:2743912/148‑1 (MQ=255)
                                                                                                                aaCTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAgg                                 >  1:2579106/1‑148 (MQ=255)
                                                                                                                 aCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgc                                >  1:448173/1‑148 (MQ=255)
                                                                                                                 aCTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgc                                >  1:1236214/1‑148 (MQ=255)
                                                                                                                  cTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcg                               >  2:2879246/1‑148 (MQ=255)
                                                                                                                  cTCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcg                               <  2:1503800/148‑1 (MQ=255)
                                                                                                                  cTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcg                               >  1:1929082/1‑148 (MQ=255)
                                                                                                                   tCGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGgcgc                              <  2:901930/148‑1 (MQ=255)
                                                                                                                   tCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACTACAGCAACGTCTTGAACAGgcgc                              <  2:2073335/148‑1 (MQ=255)
                                                                                                                    cGATTTTCTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCt                             <  2:715916/148‑1 (MQ=255)
                                                                                                                       ttttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgat                          <  1:1758756/148‑1 (MQ=255)
                                                                                                                         ttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgatga                        <  1:686576/148‑1 (MQ=255)
                                                                                                                         ttCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCtgatga                        >  2:1412959/1‑148 (MQ=255)
                                                                                                                           cTACGGTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAcc                      <  1:1287674/148‑1 (MQ=255)
                                                                                                                            tACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGAccc                     >  1:1520563/1‑148 (MQ=255)
                                                                                                                              cGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCg                   >  1:1305175/1‑148 (MQ=255)
                                                                                                                              cGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCg                   >  1:1305284/1‑148 (MQ=255)
                                                                                                                                gTTGCAAACTGCTGACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGa                 >  1:2703441/1‑148 (MQ=255)
                                                                                                                                  tGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGaca               >  2:484939/1‑148 (MQ=255)
                                                                                                                                  tGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGaca               >  2:939286/1‑148 (MQ=255)
                                                                                                                                   gCAAACTGCTGAACACGCCGATTCCGGAAGAAGATAAAGACCTGCAACTGTTCCGATAACTGGGTGTAAATCCGCAGCAAACTGGCGTGCTGGCAGGGGATAACTAACTAGAGCAACGTCTTGAACAGTCGCTGTTTACCACGTacac              <  2:1587388/148‑1 (MQ=255)
                                                                                                                                      aaCTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcga           >  1:2844627/1‑148 (MQ=255)
                                                                                                                                      aaCTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcga           >  1:2844582/1‑148 (MQ=255)
                                                                                                                                       aCTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACcgac          >  2:2865133/1‑148 (MQ=255)
                                                                                                                                          gctgACCACTCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGaa       <  2:122336/148‑1 (MQ=255)
                                                                                                                                               ccACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTa  >  1:363686/1‑148 (MQ=255)
                                                                                                                                               ccACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTa  >  1:2155569/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TCGCACCATTGCGGTCGCGCGGATCATGATGCCAACCTCTTACGTGCGCCTTTCTGCCGGACGCGAGCAGATGAACGAACAGACTCAGGCGATGTGCTTTATGGCAGGCGCAAACTCGATTTTCTACGGTTGCAAACTGCTGACCACGCCGAATCCGGAAGAAGATAAAGACCTGCAACTGTTCCGCAAACTGGGGCTAAATCCGCAGCAAACTGCCGTGCTGGCAGGGGATAACGAACAACAGCAACGTCTTGAACAGGCGCTGATGACCCCGGACACCGACGAATATTA  >  CP009273/805531‑805821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑