Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 882,672 G→T 100% L42I (CTC→ATC)  ybjJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273882,6720GT96.3% 554.4 / 12.1 187L42I (CTC→ATC) ybjJputative transporter
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base T (98/82);  total (102/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 199 total aligned reads displayed.

CCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGA  >  CP009273/882527‑882817
                                                                                                                                                 |                                                                                                                                                 
   aTCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAatat                                                                                                                                              >  2:794074/1‑148 (MQ=255)
      aaTGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcgc                                                                                                                                           <  2:1172259/148‑1 (MQ=255)
      aaTGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATcgc                                                                                                                                           >  2:1441566/1‑148 (MQ=255)
        tGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCgg                                                                                                                                         >  1:1791069/1‑148 (MQ=255)
        tGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCgg                                                                                                                                         >  1:3254767/1‑148 (MQ=255)
            cAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAg                                                                                                                                     >  1:3170200/1‑148 (MQ=255)
            cAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAg                                                                                                                                     <  2:1785240/148‑1 (MQ=255)
              ggACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCa                                                                                                                                   >  2:1386376/1‑148 (MQ=255)
                aCATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAgg                                                                                                                                 <  2:523257/148‑1 (MQ=255)
                  aTCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCg                                                                                                                               <  1:3452283/148‑1 (MQ=255)
                   tCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGcat                                                                                                                              >  1:1927510/1‑146 (MQ=255)
                   tCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGt                                                                                                                              >  2:1477137/1‑148 (MQ=255)
                   tCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGt                                                                                                                              >  1:293415/1‑148 (MQ=255)
                   tCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGt                                                                                                                              >  2:3244817/1‑148 (MQ=255)
                    cGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTa                                                                                                                             <  2:626111/148‑1 (MQ=255)
                        aCCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACggg                                                                                                                         <  2:1495483/148‑1 (MQ=255)
                            ggATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGc                                                                                                                     >  1:2082796/1‑148 (MQ=255)
                             gATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGcc                                                                                                                    <  2:3349280/148‑1 (MQ=255)
                             gATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGcc                                                                                                                    <  1:1527446/148‑1 (MQ=255)
                             gATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGcc                                                                                                                    <  1:1837306/148‑1 (MQ=255)
                             gATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGcc                                                                                                                    >  1:358546/1‑148 (MQ=255)
                                gACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATCTCGCGGATAGCAGGCGTACGGGTTGCCCAg                                                                                                                 >  1:2095239/1‑148 (MQ=255)
                                gACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAg                                                                                                                 >  1:2214145/1‑148 (MQ=255)
                                 aCATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAgg                                                                                                                <  1:2770263/148‑1 (MQ=255)
                                     tACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGc                                                                                                            >  2:105408/1‑148 (MQ=255)
                                     tACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGc                                                                                                            <  1:16842/148‑1 (MQ=255)
                                        gtgtCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCAt                                                                                                         <  2:1290764/148‑1 (MQ=255)
                                          gtCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCagtc                                                                                                       >  2:3524070/1‑145 (MQ=255)
                                          gtCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTa                                                                                                       >  2:3003602/1‑148 (MQ=255)
                                           tCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTaa                                                                                                      >  2:1099044/1‑148 (MQ=255)
                                                acgCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCACAGAGAACACCGCCCAGTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGc                                                                                                 <  1:108212/146‑1 (MQ=255)
                                                aaGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGc                                                                                                 <  1:771427/148‑1 (MQ=255)
                                                 aGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGcc                                                                                                >  1:2580135/1‑148 (MQ=255)
                                                     tttCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGc                                                                                            <  2:639702/148‑1 (MQ=255)
                                                     tttCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGc                                                                                            >  1:533831/1‑148 (MQ=255)
                                                     tttCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGc                                                                                            >  2:489527/1‑148 (MQ=255)
                                                      ttCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCa                                                                                           <  1:102971/148‑1 (MQ=255)
                                                       tCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaa                                                                                          <  2:293415/148‑1 (MQ=255)
                                                         aCTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaaaa                                                                                        >  1:1904456/1‑148 (MQ=255)
                                                         aCTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaaaa                                                                                        >  1:105666/1‑148 (MQ=255)
                                                         aCTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCaaaa                                                                                        >  2:24207/1‑148 (MQ=255)
                                                            aaCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaaga                                                                                     >  1:1246730/1‑148 (MQ=255)
                                                            aaCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaaga                                                                                     <  1:3056308/148‑1 (MQ=255)
                                                             aCCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaa                                                                                    >  1:762030/1‑148 (MQ=255)
                                                              ccACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAaagaag                                                                                   >  1:1608136/1‑148 (MQ=255)
                                                               cACGCCGATCAGAGAATAACGCTCATCGAACCGATCGACAGACAAAAGAGAACACCGCCAATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGAAAAAagaaga                                                                                  <  2:3155681/148‑1 (MQ=255)
                                                                aCGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAgaagaa                                                                                 <  1:2979282/148‑1 (MQ=255)
                                                                aCGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAgaagaa                                                                                 <  1:3201080/148‑1 (MQ=255)
                                                                aCGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAgaagaa                                                                                 <  1:1818834/148‑1 (MQ=255)
                                                                 cGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATCATAGCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCCGTCACAGGCCTGGCACAAagaagacc                                                                                >  2:2614542/1‑146 (MQ=255)
                                                                 cGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGAGAGCAGGCGTACGGGTTGCCCAGGACGCCATGCCCAGGCCTGGCAAAAAGACGAAc                                                                                >  2:458805/1‑148 (MQ=255)
                                                                  gCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACa                                                                               <  1:2027572/148‑1 (MQ=255)
                                                                        cAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACa                                                                         >  2:75975/1‑148 (MQ=255)
                                                                         agagAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAg                                                                        <  2:1083239/148‑1 (MQ=255)
                                                                           agaATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCCTTAACAGGCCTGGCAAAAAGAAGAACATAAACAgcg                                                                      >  2:77696/1‑148 (MQ=255)
                                                                             aaTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGcc                                                                    <  1:1111033/148‑1 (MQ=255)
                                                                             aaTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGcc                                                                    >  1:3164155/1‑148 (MQ=255)
                                                                             aaTACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGcc                                                                    <  1:39380/148‑1 (MQ=255)
                                                                               tACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCAATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCa                                                                  <  2:3057512/148‑1 (MQ=255)
                                                                                aCCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCAATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAg                                                                 <  2:1992649/148‑1 (MQ=255)
                                                                                   gCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGtt                                                              <  2:2825945/148‑1 (MQ=255)
                                                                                        tCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCCTTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCg                                                         >  2:1479588/1‑148 (MQ=255)
                                                                                        tCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCg                                                         >  1:1198480/1‑148 (MQ=255)
                                                                                             ccGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTAGGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCa                                                    <  1:2520664/148‑1 (MQ=255)
                                                                                             ccGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCTGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCa                                                    <  2:1274128/148‑1 (MQ=255)
                                                                                              cGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCTTACGGGTTGCCCAGTACGCAATTAACCGGCCTGGCAAAAAGATGAACATAACCAGCGCCCAGGTTCGGCGTTTCaa                                                   <  1:891556/148‑1 (MQ=255)
                                                                                                aTCGACAGACCAAAGAGAACACCGCCCATTTCAGCTATCGAGACATATATAATATCGCTTTTAGCAGGCGTACGGGTTGCCCAGGACGCAATTAACCGGCATGTAAAAAAGAAGAAAATAAACAGCGCCCAGGTTCGGCGTTTCAATg                                                 <  1:1418544/148‑1 (MQ=255)
                                                                                                aTCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATg                                                 >  2:2505903/1‑148 (MQ=255)
                                                                                                 tCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGc                                                <  1:278860/148‑1 (MQ=255)
                                                                                                    aCAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCAtt                                             >  2:1519706/1‑148 (MQ=255)
                                                                                                      aGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTAc                                           <  2:2545514/148‑1 (MQ=255)
                                                                                                        aCCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGt                                         >  1:1480053/1‑148 (MQ=255)
                                                                                                            aaGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaag                                     <  1:1076874/148‑1 (MQ=255)
                                                                                                            aaGAGAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaag                                     >  1:1551706/1‑148 (MQ=255)
                                                                                                             agagAACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaaga                                    <  2:2082796/148‑1 (MQ=255)
                                                                                                              gagaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTgaagaa                                   >  1:481620/1‑148 (MQ=255)
                                                                                                               agaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCCGGTTCGGCGTTTCAATGCATTACGTGAAGAAt                                  >  1:3134449/1‑148 (MQ=255)
                                                                                                                gaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCCTTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAtt                                 >  1:461311/1‑148 (MQ=255)
                                                                                                                gaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAtt                                 >  2:851489/1‑148 (MQ=255)
                                                                                                                gaACACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAtt                                 <  2:135357/148‑1 (MQ=255)
                                                                                                                gaACACCGCCAATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGATTCAATGCATTACGTCAAGAAtt                                 >  2:3190426/1‑148 (MQ=255)
                                                                                                                 aaCACCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAAttt                                <  2:2214145/148‑1 (MQ=255)
                                                                                                                  acacCGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTa                               >  1:623013/1‑148 (MQ=255)
                                                                                                                     ccGCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACgg                            <  1:2494370/148‑1 (MQ=255)
                                                                                                                       gCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTc                          <  1:3044619/148‑1 (MQ=255)
                                                                                                                       gCCCATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTc                          <  2:547705/148‑1 (MQ=255)
                                                                                                                        cccATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCa                         >  1:2405291/1‑148 (MQ=255)
                                                                                                                         ccATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCAt                        <  1:3244696/148‑1 (MQ=255)
                                                                                                                         ccATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCAt                        <  2:606027/148‑1 (MQ=255)
                                                                                                                         ccATTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCAt                        <  1:1477137/148‑1 (MQ=255)
                                                                                                                           aTTTCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAg                      >  1:2708197/1‑148 (MQ=255)
                                                                                                                            tttCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGa                     >  1:1881680/1‑148 (MQ=255)
                                                                                                                              tCAGCGATCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATc                   <  2:1257621/148‑1 (MQ=255)
                                                                                                                                     tCGAGACAGAGATAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCaa            >  2:1004366/1‑148 (MQ=255)
                                                                                                                                      cGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCaaa           >  2:1303197/1‑148 (MQ=255)
                                                                                                                                            agagatAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaaga     <  1:187016/148‑1 (MQ=255)
                                                                                                                                             gagataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaa    <  1:105408/148‑1 (MQ=255)
                                                                                                                                              agataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaag   >  2:328861/1‑148 (MQ=255)
                                                                                                                                              agataATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATaagaag   <  2:55147/148‑1 (MQ=255)
                                                                                                                                               gagaATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAagaaga  <  2:358547/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAACCACGCCGAGCAGAGAATACCGCTCATCGAACCGATCGACAGACCAAAGAGAACACCGCCCATTTCAGCGATCGAGACAGAGAGAATATCGCGGATAGCAGGCGTACGGGTTGCCCAGGACGCCATTAACAGGCCTGGCAAAAAGAAGAACATAAACAGCGCCCAGGTTCGGCGTTTCAATGCATTACGTGAAGAATTTACGGTCATAGATCACGTCAAAATAAGAAGA  >  CP009273/882527‑882817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑