Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 994,328 G→T 100% G128V (GGA→GTA)  elfD → putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273994,3280GT98.4% 617.8 / 0.7 192G128V (GGA→GTA) elfDputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (94/94);  total (95/97)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

TTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA  >  CP009273/994184‑994472
                                                                                                                                                |                                                                                                                                                
ttaCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCACCAACGAAGCGGAAAGAGGTaaa                                                                                                                                               <  2:2888323/148‑1 (MQ=255)
 taCACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAAt                                                                                                                                              >  2:1801547/1‑148 (MQ=255)
    accaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAcc                                                                                                                                           <  2:124547/148‑1 (MQ=255)
    accaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAcc                                                                                                                                           <  1:2295410/148‑1 (MQ=255)
     ccaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAccc                                                                                                                                          >  2:2640999/1‑148 (MQ=255)
     ccaccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATAccc                                                                                                                                          >  1:2469904/1‑148 (MQ=255)
        ccGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGa                                                                                                                                       >  1:1234598/1‑148 (MQ=255)
          gCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAg                                                                                                                                     <  2:1866000/148‑1 (MQ=255)
           cTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGa                                                                                                                                    <  2:864936/148‑1 (MQ=255)
            tATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGAt                                                                                                                                   <  1:188398/148‑1 (MQ=255)
              tttGTGCTGAATCCGGCAAATGAGAATCTGGTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTg                                                                                                                                 >  1:3268085/1‑148 (MQ=255)
               ttGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGc                                                                                                                                >  1:3071952/1‑148 (MQ=255)
               ttGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGc                                                                                                                                >  1:850936/1‑148 (MQ=255)
               ttGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGc                                                                                                                                <  2:924614/148‑1 (MQ=255)
                 gtgCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGTAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCca                                                                                                                              <  1:1178797/148‑1 (MQ=255)
                 gtgCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCca                                                                                                                              <  1:1272311/148‑1 (MQ=255)
                  tgCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcac                                                                                                                             >  1:270447/1‑148 (MQ=255)
                    cTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcacac                                                                                                                           <  1:3427327/148‑1 (MQ=255)
                     tGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCcacaca                                                                                                                          >  1:1038030/1‑148 (MQ=255)
                      gAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACaa                                                                                                                         <  1:852267/148‑1 (MQ=255)
                        aTCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACACTTTTCTTCACTAGCGTACGGGCAGTCCCTACAACAACGAAGCGGAAATAGGTAAAAACCCTGAAGATTGCAACACAAAg                                                                                                                       >  1:3210476/1‑148 (MQ=255)
                          ccGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCg                                                                                                                     <  2:3211938/148‑1 (MQ=255)
                           cGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGt                                                                                                                    <  1:3245708/148‑1 (MQ=255)
                            ggCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGtc                                                                                                                   <  1:3316749/148‑1 (MQ=255)
                                  tGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCaaa                                                                                                             >  2:2804954/1‑148 (MQ=255)
                                  tGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCaaa                                                                                                             >  1:3462761/1‑148 (MQ=255)
                                  tGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCaaa                                                                                                             >  2:3398390/1‑148 (MQ=255)
                                  tGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCaaa                                                                                                             <  2:641529/148‑1 (MQ=255)
                                    agaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACt                                                                                                           >  2:1635315/1‑148 (MQ=255)
                                     gaATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACtt                                                                                                          >  1:160974/1‑148 (MQ=255)
                                      aaTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                         >  1:963467/1‑148 (MQ=255)
                                      aaTCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACttt                                                                                                         >  1:963152/1‑148 (MQ=255)
                                        tCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTc                                                                                                       >  2:2324804/1‑148 (MQ=255)
                                        tCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTc                                                                                                       >  2:2325280/1‑148 (MQ=255)
                                         cTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCt                                                                                                      <  2:170864/148‑1 (MQ=255)
                                         cTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCt                                                                                                      <  2:170754/148‑1 (MQ=255)
                                            ttACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGc                                                                                                   >  1:3147396/1‑148 (MQ=255)
                                             tACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCg                                                                                                  >  1:536343/1‑148 (MQ=255)
                                                       gTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGt                                                                                        <  1:3372167/148‑1 (MQ=255)
                                                       gTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGt                                                                                        <  1:831292/148‑1 (MQ=255)
                                                        tACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGAc                                                                                                >  1:1656347/1‑139 (MQ=255)
                                                        tACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGAc                                                                                                <  2:1656347/139‑1 (MQ=255)
                                                            ttGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGc                                                                                   >  2:1139162/1‑148 (MQ=255)
                                                             tGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCg                                                                                  >  2:2503338/1‑148 (MQ=255)
                                                             tGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCg                                                                                  >  2:956795/1‑148 (MQ=255)
                                                              ggAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGt                                                                                 <  1:2066210/148‑1 (MQ=255)
                                                                   gcCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATccc                                                                            <  2:2911698/148‑1 (MQ=255)
                                                                     cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCtt                                                                          <  2:787853/148‑1 (MQ=255)
                                                                     cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCtt                                                                          <  2:787640/148‑1 (MQ=255)
                                                                     cGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCtt                                                                          <  2:3408319/148‑1 (MQ=255)
                                                                       ttGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                        >  2:2751274/1‑148 (MQ=255)
                                                                       ttGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATAGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAg                                                                        <  2:1940700/148‑1 (MQ=255)
                                                                        tGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAgg                                                                       <  1:1513809/148‑1 (MQ=255)
                                                                          gCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCg                                                                     <  1:133524/148‑1 (MQ=255)
                                                                          gCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCg                                                                     <  1:2639794/148‑1 (MQ=255)
                                                                           cGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGa                                                                    >  1:415390/1‑148 (MQ=255)
                                                                             aaaGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAgg                                                                  >  2:2055459/1‑148 (MQ=255)
                                                                               aGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCt                                                                <  2:2537398/148‑1 (MQ=255)
                                                                                gACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTc                                                               <  2:2981822/148‑1 (MQ=255)
                                                                                 aCAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTcc                                                              >  2:244625/1‑148 (MQ=255)
                                                                                   agagAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCgg                                                            <  1:1826633/148‑1 (MQ=255)
                                                                                   agagAAACCCGTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCgg                                                            <  2:1847775/148‑1 (MQ=255)
                                                                                    gagaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGc                                                           >  1:2747104/1‑148 (MQ=255)
                                                                                     agaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACACTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCg                                                          >  1:2280152/1‑148 (MQ=255)
                                                                                      gaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGa                                                         >  1:1966926/1‑148 (MQ=255)
                                                                                      gaAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGa                                                         >  2:3048133/1‑148 (MQ=255)
                                                                                       aaaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaa                                                        <  2:178348/148‑1 (MQ=255)
                                                                                       aaaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaa                                                        >  2:2558826/1‑148 (MQ=255)
                                                                                       aaaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaa                                                        >  1:2768139/1‑148 (MQ=255)
                                                                                        aaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaaa                                                       <  1:1720420/148‑1 (MQ=255)
                                                                                        aaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaaa                                                       <  1:1714229/148‑1 (MQ=255)
                                                                                        aaCCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCAGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGaaa                                                       <  1:28928/148‑1 (MQ=255)
                                                                                             ttttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCg                                                  <  2:1225049/148‑1 (MQ=255)
                                                                                             ttttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCg                                                  >  1:2983198/1‑148 (MQ=255)
                                                                                              tttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGt                                                 <  2:3507514/148‑1 (MQ=255)
                                                                                              tttCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGt                                                 <  2:3531299/148‑1 (MQ=255)
                                                                                               ttcttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGtt                                                <  1:263464/148‑1 (MQ=255)
                                                                                                tcttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTg                                               >  1:1721125/1‑148 (MQ=255)
                                                                                                tcttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTg                                               >  1:2870768/1‑148 (MQ=255)
                                                                                                 cttcACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGc                                              <  1:179055/148‑1 (MQ=255)
                                                                                                    cACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACt                                           >  2:1299459/1‑148 (MQ=255)
                                                                                                        aGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATNAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGt                                       <  1:1805581/148‑1 (MQ=255)
                                                                                                        aGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGt                                       >  2:1268694/1‑148 (MQ=255)
                                                                                                         gCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTc                                      <  2:668105/148‑1 (MQ=255)
                                                                                                         gCGGACGCGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACACAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTc                                      <  2:2449360/148‑1 (MQ=255)
                                                                                                          cGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCa                                     >  1:45272/1‑148 (MQ=255)
                                                                                                                ggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGAc                               <  2:724656/148‑1 (MQ=255)
                                                                                                                ggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGAc                               <  2:1742678/148‑1 (MQ=255)
                                                                                                                ggCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGAc                               >  1:1879797/1‑148 (MQ=255)
                                                                                                                 gCAGTCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACa                              <  2:963152/148‑1 (MQ=255)
                                                                                                                     tCCCTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGt                          >  2:3299516/1‑148 (MQ=255)
                                                                                                                     tCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGt                          <  2:3167944/148‑1 (MQ=255)
                                                                                                                      cccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGtt                         >  1:710580/1‑148 (MQ=255)
                                                                                                                       ccTTCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTa                        >  1:2343026/1‑148 (MQ=255)
                                                                                                                         ttCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACg                      >  1:1032558/1‑148 (MQ=255)
                                                                                                                          tCAACAACGAAGCGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACgg                     <  1:2640999/148‑1 (MQ=255)
                                                                                                                                      cGGAAAGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCaa         >  2:2169946/1‑148 (MQ=255)
                                                                                                                                         aaaGAGGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAacac      <  1:690234/148‑1 (MQ=255)
                                                                                                                                            gagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCtt   >  2:1899191/1‑148 (MQ=255)
                                                                                                                                            gagGTAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCtt   >  1:1123139/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTACACCACCGCTATTTGTGCTGAATCCGGCAAATGAGAATCTGTTACGCATTATGTACATTGGAGCGCCGTTGGCGAAAGACAGAGAAACCCTTTTCTTCACTAGCGTACGGGCAGTCCCTTCAACAACGAAGCGGAAAGAGGGAAATACCCTGAAGATTGCCACACAAAGCGTCATCAAACTTTTCTGGCGACCAAAAGGTTTAGCGTATCCCTTAGGCGAGGCTCCGGCGAAACTGCGTTGCACTTCGTCAGCTGACATGGTTACGGTCAGTAACCCAACACCTTA  >  CP009273/994184‑994472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑