Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,024,703 G→T 100% G241G (GGC→GGA)  rlmI ← 23S rRNA m(5)C1962 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,024,7030GT95.5% 650.0 / 19.4 220G241G (GGC→GGA) rlmI23S rRNA m(5)C1962 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base T (111/99);  total (116/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 220 total aligned reads displayed.

CGATCGCGATAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCAC  >  CP009273/1024557‑1024846
                                                                                                                                                  |                                                                                                                                               
cgatcgcgatAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCGc                                                                                                                                                <  1:3550162/148‑1 (MQ=255)
      cgatAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCa                                                                                                                                          >  1:474981/1‑148 (MQ=255)
       gatAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCACATCAg                                                                                                                                         <  2:3236617/148‑1 (MQ=255)
         tAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTg                                                                                                                                       >  1:170462/1‑148 (MQ=255)
          aaGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGc                                                                                                                                      >  1:2495629/1‑148 (MQ=255)
           aGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGcc                                                                                                                                     >  2:3521131/1‑148 (MQ=255)
           aGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGcc                                                                                                                                     >  2:2663992/1‑148 (MQ=255)
            gTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCg                                                                                                                                    >  1:812130/1‑148 (MQ=255)
            gTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCg                                                                                                                                    >  2:2199999/1‑148 (MQ=255)
                 cagcaATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACg                                                                                                                               >  2:1786392/1‑148 (MQ=255)
                   gcaATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGc                                                                                                                             >  1:759957/1‑148 (MQ=255)
                    caATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCg                                                                                                                            >  1:1001019/1‑148 (MQ=255)
                      aTTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGaa                                                                                                                          <  2:908874/148‑1 (MQ=255)
                        ttAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAac                                                                                                                        >  1:2906590/1‑148 (MQ=255)
                        ttAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAac                                                                                                                        >  1:2925549/1‑148 (MQ=255)
                        ttAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAac                                                                                                                        >  2:1192998/1‑148 (MQ=255)
                          aaaGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAacca                                                                                                                      <  2:2638851/148‑1 (MQ=255)
                           aaGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAaccac                                                                                                                     <  1:2911797/148‑1 (MQ=255)
                            aGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAaccacc                                                                                                                    <  2:258409/148‑1 (MQ=255)
                            aGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAaccacc                                                                                                                    >  1:1425288/1‑148 (MQ=255)
                             gACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCg                                                                                                                   <  1:3201674/148‑1 (MQ=255)
                             gACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCg                                                                                                                   >  1:1858206/1‑148 (MQ=255)
                              aCATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCgg                                                                                                                  >  1:1722514/1‑148 (MQ=255)
                               catcatCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGt                                                                                                                 >  2:896680/1‑148 (MQ=255)
                               catcatCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGt                                                                                                                 <  2:961710/148‑1 (MQ=255)
                                 tcatcaCGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGtat                                                                                                               >  1:1478844/1‑148 (MQ=255)
                                 tcatcaCGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGtat                                                                                                               >  1:1506172/1‑148 (MQ=255)
                                     caCGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGga                                                                                                           <  2:3078069/148‑1 (MQ=255)
                                     caCGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGga                                                                                                           >  2:2490273/1‑148 (MQ=255)
                                     caCGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGga                                                                                                           >  2:2300512/1‑148 (MQ=255)
                                        ggACAAACTCAGCCTTGCTCAGATCAAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGaa                                                                                                        <  1:3317524/148‑1 (MQ=255)
                                          aCAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAAc                                                                                                      <  1:324027/148‑1 (MQ=255)
                                          aCAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAAc                                                                                                      <  1:2119507/148‑1 (MQ=255)
                                           cAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACa                                                                                                     <  1:3446774/148‑1 (MQ=255)
                                            aaaCTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAg                                                                                                    >  1:3002602/1‑148 (MQ=255)
                                            aaaCTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAg                                                                                                    <  2:2563716/148‑1 (MQ=255)
                                             aaCTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGt                                                                                                   >  1:105544/1‑148 (MQ=255)
                                             aaCTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGt                                                                                                   <  2:1377083/148‑1 (MQ=255)
                                               cTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTc                                                                                                 <  1:2046621/148‑1 (MQ=255)
                                                   gCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGca                                                                                             >  2:1717256/1‑148 (MQ=255)
                                                    ccTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGcac                                                                                            >  2:106594/1‑148 (MQ=255)
                                                        gCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACGGTTCAGCACACGt                                                                                        <  1:2148375/148‑1 (MQ=255)
                                                         cTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGtt                                                                                       >  1:3514295/1‑148 (MQ=255)
                                                           cAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTa                                                                                     >  2:2976157/1‑148 (MQ=255)
                                                           cAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTa                                                                                     >  2:633525/1‑148 (MQ=255)
                                                           cAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTa                                                                                     >  1:451236/1‑148 (MQ=255)
                                                            aGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAACCAGTTCAGCACACGGTTAt                                                                                    >  2:2382626/1‑148 (MQ=255)
                                                            aGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTAt                                                                                    >  2:2306411/1‑148 (MQ=255)
                                                            aGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTAt                                                                                    >  2:519963/1‑148 (MQ=255)
                                                            aGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTAt                                                                                    <  2:1981565/148‑1 (MQ=255)
                                                               tCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTAtttt                                                                                 >  1:492866/1‑148 (MQ=255)
                                                                  aGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCaa                                                                              >  1:3266991/1‑148 (MQ=255)
                                                                  aGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCaa                                                                              <  2:2954926/148‑1 (MQ=255)
                                                                  aGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCaa                                                                              <  2:1035520/148‑1 (MQ=255)
                                                                       gTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAg                                                                         <  1:637047/148‑1 (MQ=255)
                                                                        ttCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAgc                                                                        >  1:741364/1‑148 (MQ=255)
                                                                              tCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTCTCAACGCTGACAACCTGGCTGCAACCTACCATCCGGGCCGATACTGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCggg                                                                  <  2:1841925/148‑1 (MQ=255)
                                                                              tCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCggg                                                                  <  1:3048691/148‑1 (MQ=255)
                                                                                    ttCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCa                                                            >  1:2268170/1‑148 (MQ=255)
                                                                                     tCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAg                                                           >  2:297602/1‑148 (MQ=255)
                                                                                      cTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAgg                                                          >  2:2363165/1‑148 (MQ=255)
                                                                                      cTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAgg                                                          <  2:2307088/148‑1 (MQ=255)
                                                                                         ccGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAggcgg                                                       <  1:2260385/148‑1 (MQ=255)
                                                                                         ccGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAggcgg                                                       <  1:3415669/148‑1 (MQ=255)
                                                                                           gTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCt                                                     <  2:3478855/148‑1 (MQ=255)
                                                                                            tGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTa                                                    >  2:1496412/1‑148 (MQ=255)
                                                                                              cAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATc                                                  >  2:1252459/1‑148 (MQ=255)
                                                                                                atatCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCAc                                                >  1:3205743/1‑148 (MQ=255)
                                                                                                atatCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCAc                                                >  1:3531740/1‑148 (MQ=255)
                                                                                                  atCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGc                                              <  2:812130/148‑1 (MQ=255)
                                                                                                   tCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCt                                             <  1:3265452/148‑1 (MQ=255)
                                                                                                   tCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCt                                             <  1:1786392/148‑1 (MQ=255)
                                                                                                   tCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCt                                             <  1:2680025/148‑1 (MQ=255)
                                                                                                     cAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTgg                                           <  1:3353051/148‑1 (MQ=255)
                                                                                                      aGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGt                                          >  2:2739440/1‑148 (MQ=255)
                                                                                                       gcgcTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTc                                         >  1:1510135/1‑148 (MQ=255)
                                                                                                       gcgcTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTc                                         <  2:759957/148‑1 (MQ=255)
                                                                                                       gcgcTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTc                                         >  1:352206/1‑148 (MQ=255)
                                                                                                       gcgcTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTc                                         <  2:1425288/148‑1 (MQ=255)
                                                                                                        cgcgTCCTGGGAGGTAGCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTcc                                        <  2:299824/148‑1 (MQ=255)
                                                                                                        cgcTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTcc                                        <  2:165217/148‑1 (MQ=255)
                                                                                                             ccTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGgta                                   <  2:2140618/148‑1 (MQ=255)
                                                                                                                 ggAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGtagtag                               >  1:1099247/1‑148 (MQ=255)
                                                                                                                  gAGGTATCAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGc                              <  2:886535/148‑1 (MQ=255)
                                                                                                                         cAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGtttt                       >  1:2890947/1‑148 (MQ=255)
                                                                                                                         cAACGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGtttt                       <  1:3447245/148‑1 (MQ=255)
                                                                                                                          aaCGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTtg                      <  1:573778/148‑1 (MQ=255)
                                                                                                                            cGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTtgtg                    <  2:1439814/148‑1 (MQ=255)
                                                                                                                            cGCTGACAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTtgtg                    >  1:2588213/1‑148 (MQ=255)
                                                                                                                                 aCAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGt               >  1:196122/1‑148 (MQ=255)
                                                                                                                                  cAACCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTGTTGTGTCCGTg              >  2:277174/1‑148 (MQ=255)
                                                                                                                                    aCCTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCt            >  1:2306795/1‑148 (MQ=255)
                                                                                                                                     ccTGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTg           >  2:1746942/1‑148 (MQ=255)
                                                                                                                                       tGGCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGaa         <  1:896680/148‑1 (MQ=255)
                                                                                                                                        ggCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAat        <  1:2711266/148‑1 (MQ=255)
                                                                                                                                        ggCTGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAat        <  1:2300512/148‑1 (MQ=255)
                                                                                                                                         gCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAata       <  1:3360214/148‑1 (MQ=255)
                                                                                                                                           tGCAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATc     <  2:2178090/148‑1 (MQ=255)
                                                                                                                                             cAACCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATcca   <  2:2871432/148‑1 (MQ=255)
                                                                                                                                              aaCCTCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATccac  <  2:2668967/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CGATCGCGATAAGTACGCAGCAATTTAAAGACATCATCACGGACAAACTCAGCCTTGCTCAGATCCAGTTTGTTCAGCTCAACGTTCTGCCGTGCAATATCCAGCGCTTCCTGGGAGGTATCAACGCTGACAACCTGGCTGCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCAC  >  CP009273/1024557‑1024846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑