Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,031,861 G→A 10.3% G18S (GGC→AGC)  hyaE → putative HyaA chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,031,8610GA10.3% 605.4 / 40.2 185G18S (GGC→AGC) hyaEputative HyaA chaperone
Reads supporting (aligned to +/- strand):  ref base G (93/72);  new base A (14/5);  total (108/77)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

TAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCC  >  CP009273/1031714‑1032006
                                                                                                                                                   |                                                                                                                                                 
tAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACTCCATTTGATGCGTTGTGGCAACGAATGCTGGcgcgcg                                                                                                                                                   >  1:1027059/1‑148 (MQ=255)
 aaTGAATCGCGCTGTCTCAATTATGACTGTCGGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCgg                                                                                                                                                  <  2:3395500/148‑1 (MQ=255)
  aTGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGc                                                                                                                                                 <  1:313132/148‑1 (MQ=255)
    gAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCCACGAATGCTGGCGCGCGGCTg                                                                                                                                               >  2:1067464/1‑148 (MQ=255)
    gAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTg                                                                                                                                               >  2:3215732/1‑148 (MQ=255)
     aaTCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTgg                                                                                                                                              <  1:2658370/148‑1 (MQ=255)
      aTCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGa                                                                                                                                             >  2:701507/1‑148 (MQ=255)
      aTCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGa                                                                                                                                             >  2:108062/1‑148 (MQ=255)
      aTCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGa                                                                                                                                             >  2:2578791/1‑148 (MQ=255)
       tCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGAc                                                                                                                                            >  2:1544238/1‑148 (MQ=255)
         gcgcTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGc                                                                                                                                          <  2:1700558/148‑1 (MQ=255)
               tctcAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCcagtca                                                                                                                                    >  1:3437950/1‑148 (MQ=255)
               tctcAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCcagtca                                                                                                                                    >  2:2644201/1‑148 (MQ=255)
               tctcAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCcagtca                                                                                                                                    >  1:843416/1‑148 (MQ=255)
                ctcAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCcagtcag                                                                                                                                   <  2:2742090/148‑1 (MQ=255)
                     ttATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAAt                                                                                                                              >  1:2411406/1‑148 (MQ=255)
                      tATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATc                                                                                                                             <  2:3542718/148‑1 (MQ=255)
                        tGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATccc                                                                                                                           >  1:1849551/1‑148 (MQ=255)
                        tGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATccc                                                                                                                           >  1:1956469/1‑148 (MQ=255)
                           ctgtctgtCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTc                                                                                                                        <  2:1232996/148‑1 (MQ=255)
                             gtctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCtt                                                                                                                      >  2:592805/1‑148 (MQ=255)
                             gtctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCtt                                                                                                                      >  2:1869079/1‑148 (MQ=255)
                              tctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTg                                                                                                                     >  1:1047890/1‑148 (MQ=255)
                              tctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTg                                                                                                                     <  1:2513474/148‑1 (MQ=255)
                              tctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTg                                                                                                                     >  2:2654773/1‑148 (MQ=255)
                              tctgtcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTg                                                                                                                     <  1:2143390/148‑1 (MQ=255)
                                  tcGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGTGCAGGGATGAGCAACGACACGCCATTTTATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTgacga                                                                                                                 <  1:3314255/148‑1 (MQ=255)
                                     aTGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTg                                                                                                              >  1:2648394/1‑148 (MQ=255)
                                      tGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTgg                                                                                                             <  1:1068406/148‑1 (MQ=255)
                                      tGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTgg                                                                                                             >  2:615302/1‑148 (MQ=255)
                                      tGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTgg                                                                                                             >  2:615191/1‑148 (MQ=255)
                                      tGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCAGTTGATGCGTTGTGGCACCGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTgg                                                                                                             >  2:232265/1‑148 (MQ=255)
                                       ggAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGc                                                                                                            >  1:816468/1‑148 (MQ=255)
                                        gAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCt                                                                                                           <  1:398633/148‑1 (MQ=255)
                                           aaTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTAc                                                                                                        <  2:1457285/148‑1 (MQ=255)
                                           aaTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTAc                                                                                                        >  2:2998500/1‑148 (MQ=255)
                                           aaTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTAc                                                                                                        >  2:2232428/1‑148 (MQ=255)
                                            aTTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACg                                                                                                       >  1:2658589/1‑148 (MQ=255)
                                              tACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCa                                                                                                     <  2:1197598/148‑1 (MQ=255)
                                              tACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCa                                                                                                     <  2:1128372/148‑1 (MQ=255)
                                                     gCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCa                                                                                              >  2:16849/1‑148 (MQ=255)
                                                      cGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAg                                                                                             <  1:1664047/148‑1 (MQ=255)
                                                        ttCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCNACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGAc                                                                                           <  1:1807706/148‑1 (MQ=255)
                                                        ttCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGAc                                                                                           <  1:338701/148‑1 (MQ=255)
                                                           gCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGc                                                                                        >  1:592532/1‑148 (MQ=255)
                                                            cTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCg                                                                                       <  2:439598/148‑1 (MQ=255)
                                                              tGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCgtg                                                                                     <  2:553758/148‑1 (MQ=255)
                                                                  ccAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGgtgt                                                                                 >  1:2270409/1‑148 (MQ=255)
                                                                   cAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGtt                                                                                <  2:2452681/148‑1 (MQ=255)
                                                                   cAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTAAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGtt                                                                                >  2:2285254/1‑148 (MQ=255)
                                                                    aGTACCGGATGACCCAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGtta                                                                               <  1:3433623/148‑1 (MQ=255)
                                                                           gATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCa                                                                        >  2:871171/1‑148 (MQ=255)
                                                                            aTGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAg                                                                       >  2:2508471/1‑148 (MQ=255)
                                                                            aTGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAg                                                                       >  1:2593959/1‑148 (MQ=255)
                                                                                 acaGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGAcc                                                                  <  2:1478556/148‑1 (MQ=255)
                                                                                 acaGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGAcc                                                                  <  2:1478432/148‑1 (MQ=255)
                                                                                    ggaggaGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGa                                                               >  1:1736985/1‑148 (MQ=255)
                                                                                     gaggagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaa                                                              >  1:2368512/1‑148 (MQ=255)
                                                                                     gaggagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaa                                                              >  2:191383/1‑148 (MQ=255)
                                                                                     gaggagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaa                                                              >  1:3070578/1‑148 (MQ=255)
                                                                                     gaggagCAGGGATGAGCAACGACACGCCATTTGATGCGTGGTGGCAACGAAGGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaa                                                              <  1:1594067/148‑1 (MQ=255)
                                                                                     gaggagCAGGGATGAGCAACGACACGCAATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGaa                                                              <  1:3352712/148‑1 (MQ=255)
                                                                                       ggagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAac                                                            >  1:1506377/1‑148 (MQ=255)
                                                                                       ggagCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAac                                                            >  1:1156124/1‑148 (MQ=255)
                                                                                         agCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgc                                                          <  1:409350/148‑1 (MQ=255)
                                                                                               gATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCa                                                    >  1:354196/1‑148 (MQ=255)
                                                                                               gATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCa                                                    >  1:1812333/1‑148 (MQ=255)
                                                                                                aTGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCag                                                   >  1:2836136/1‑148 (MQ=255)
                                                                                                    gCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGt                                               <  2:354196/148‑1 (MQ=255)
                                                                                                     cAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTc                                              <  2:3026505/148‑1 (MQ=255)
                                                                                                     cAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTc                                              >  2:3266894/1‑148 (MQ=255)
                                                                                                       aCGACACGCCTTTTTATGGGTTGTGGCAACGACTGCTGGCGCGGGGCGGGACGCCAGTCTGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAg                                            <  1:911780/148‑1 (MQ=255)
                                                                                                        cGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGc                                           >  1:2246011/1‑148 (MQ=255)
                                                                                                               ccttttGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATc                                    <  1:1078885/145‑1 (MQ=255)
                                                                                                               ccATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATc                                    >  2:1809919/1‑148 (MQ=255)
                                                                                                                   ttGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt                                >  1:101781/1‑148 (MQ=255)
                                                                                                                   ttGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt                                >  2:3089537/1‑148 (MQ=255)
                                                                                                                   ttGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt                                <  1:2884304/148‑1 (MQ=255)
                                                                                                                   ttGATGCGTTGTGGCAACGAAGGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGt                                <  1:3380469/148‑1 (MQ=255)
                                                                                                                        gCGTTGTGGCAACGACTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGa                           <  1:2167452/148‑1 (MQ=255)
                                                                                                                        gCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGa                           <  1:2998500/148‑1 (MQ=255)
                                                                                                                        gCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGa                           <  1:1544238/148‑1 (MQ=255)
                                                                                                                        gCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGa                           <  2:3270191/148‑1 (MQ=255)
                                                                                                                        gCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGa                           >  1:218662/1‑148 (MQ=255)
                                                                                                                         cGTTGTGGCAACGTTTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                          <  1:1632980/148‑1 (MQ=255)
                                                                                                                         cGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                          >  2:2063099/1‑148 (MQ=255)
                                                                                                                         cGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                          >  2:2063172/1‑148 (MQ=255)
                                                                                                                         cGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                          >  2:3380470/1‑148 (MQ=255)
                                                                                                                         cGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                          <  2:843416/148‑1 (MQ=255)
                                                                                                                         cGTTGTGGCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAt                          >  1:814989/1‑148 (MQ=255)
                                                                                                                          gTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGAtt                         <  2:2387217/148‑1 (MQ=255)
                                                                                                                            tgtgGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTgg                       >  2:2748546/1‑148 (MQ=255)
                                                                                                                                gCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                   >  1:1198729/1‑148 (MQ=255)
                                                                                                                                gCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATACTCCGGTAATGATTGGCGaa                   >  1:1891358/1‑148 (MQ=255)
                                                                                                                                gCAACGAATGCTGGCGCGCAGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                   <  1:166054/148‑1 (MQ=255)
                                                                                                                                   aCGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                >  1:2335060/1‑148 (MQ=255)
                                                                                                                                      aaTGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTg             <  1:3218337/148‑1 (MQ=255)
                                                                                                                                         gCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcgc          >  1:732276/1‑148 (MQ=255)
                                                                                                                                                cgcgGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTc   <  1:600136/148‑1 (MQ=255)
                                                                                                                                                 gcgGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTcc  <  2:2663136/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TAATGAATCGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGCCAGTACCGGATGACACAGGAGGAGCAGGGATGAGCAACGACACGCCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCC  >  CP009273/1031714‑1032006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑