Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 102,769 G→T 100% V326V (GTG→GTT)  ftsZ → GTP‑binding tubulin‑like cell division protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273102,7690GT96.5% 708.7 / 14.4 230V326V (GTG→GTT) ftsZGTP‑binding tubulin‑like cell division protein
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (138/84);  total (142/88)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 230 total aligned reads displayed.

TAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGATATCC  >  CP009273/102624‑102914
                                                                                                                                                 |                                                                                                                                                 
taggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAc                                                                                                                                                 <  1:1002135/148‑1 (MQ=255)
taggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTAc                                                                                                                                                 <  2:2973910/148‑1 (MQ=255)
  ggtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCa                                                                                                                                               <  2:3170879/148‑1 (MQ=255)
   gtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCaa                                                                                                                                              <  2:155890/148‑1 (MQ=255)
   gtaACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCaa                                                                                                                                              <  2:1951822/148‑1 (MQ=255)
    taACACCATCCGTGCATTTTCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAAt                                                                                                                                             <  2:2233734/148‑1 (MQ=255)
     aaCACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATa                                                                                                                                            <  1:3540443/148‑1 (MQ=255)
      acacCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATaa                                                                                                                                           >  2:2090795/1‑148 (MQ=255)
         ccATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCa                                                                                                                                        <  2:176249/148‑1 (MQ=255)
           aTCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAgg                                                                                                                                      >  2:3312680/1‑148 (MQ=255)
            tCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGt                                                                                                                                     >  2:1665217/1‑148 (MQ=255)
             ccGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGtt                                                                                                                                    >  1:2540607/1‑148 (MQ=255)
             ccGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGtt                                                                                                                                    >  1:746121/1‑148 (MQ=255)
             ccGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGtt                                                                                                                                    <  1:2743288/148‑1 (MQ=255)
             ccGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGtt                                                                                                                                    >  2:1190257/1‑148 (MQ=255)
                 gCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTcagc                                                                                                                                >  1:2818731/1‑148 (MQ=255)
                    tttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCagcagc                                                                                                                             <  2:2861520/148‑1 (MQ=255)
                     ttGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGcc                                                                                                                            >  2:302905/1‑148 (MQ=255)
                         ttCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTg                                                                                                                        <  1:2355067/148‑1 (MQ=255)
                          tCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGa                                                                                                                       >  1:3462750/1‑148 (MQ=255)
                          tCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGATCAGCAGCCAGTGa                                                                                                                       >  2:859399/1‑148 (MQ=255)
                            cGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATg                                                                                                                     >  2:3299609/1‑148 (MQ=255)
                            cGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATg                                                                                                                     <  2:832953/148‑1 (MQ=255)
                            cGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATg                                                                                                                     <  2:1799358/148‑1 (MQ=255)
                            cGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGCCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATg                                                                                                                     <  2:833216/148‑1 (MQ=255)
                              aCAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGa                                                                                                                   >  1:3057301/1‑148 (MQ=255)
                              aCAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGa                                                                                                                   >  2:2720651/1‑148 (MQ=255)
                              aCAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGa                                                                                                                   >  1:3351995/1‑148 (MQ=255)
                              aCAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGAGATGAAGGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGa                                                                                                                   <  1:295566/148‑1 (MQ=255)
                                aaCGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATc                                                                                                                 >  1:456582/1‑148 (MQ=255)
                                    cgACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCGTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTa                                                                                                             <  1:967596/148‑1 (MQ=255)
                                      aCTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTAcc                                                                                                           >  1:2683334/1‑148 (MQ=255)
                                       cTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACca                                                                                                          <  1:3146767/148‑1 (MQ=255)
                                           ggTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagcag                                                                                                      >  1:1058504/1‑148 (MQ=255)
                                           ggTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACcagcag                                                                                                      <  1:1947841/148‑1 (MQ=255)
                                                 cGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATggg                                                                                                <  2:457486/148‑1 (MQ=255)
                                                 cGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATggg                                                                                                >  2:630788/1‑148 (MQ=255)
                                                 cGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATggg                                                                                                >  2:1857155/1‑148 (MQ=255)
                                                 cGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATggg                                                                                                >  2:1152843/1‑148 (MQ=255)
                                                  ggTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGa                                                                                               >  2:3177739/1‑148 (MQ=255)
                                                  ggTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGa                                                                                               >  1:1402360/1‑148 (MQ=255)
                                                     aCTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATgg                                                                                            >  2:1899622/1‑148 (MQ=255)
                                                       ttCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCt                                                                                          >  1:1622059/1‑148 (MQ=255)
                                                          tctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCg                                                                                       >  1:3318116/1‑148 (MQ=255)
                                                           ctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGc                                                                                      >  1:2070539/1‑148 (MQ=255)
                                                           ctTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGc                                                                                      >  1:2559021/1‑148 (MQ=255)
                                                               aCCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGAc                                                                                  >  1:3184833/1‑148 (MQ=255)
                                                                     atatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGa                                                                            >  1:1231510/1‑148 (MQ=255)
                                                                      tatGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAg                                                                           >  2:1603476/1‑148 (MQ=255)
                                                                       atgaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGc                                                                          <  2:2646034/148‑1 (MQ=255)
                                                                       atgaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGc                                                                          >  1:862813/1‑148 (MQ=255)
                                                                       atgaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGc                                                                          >  2:186772/1‑148 (MQ=255)
                                                                        tgaatgaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCa                                                                         >  1:2982252/1‑148 (MQ=255)
                                                                             gaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGc                                                                    >  1:3478332/1‑148 (MQ=255)
                                                                             gaCGAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGc                                                                    <  1:2525980/148‑1 (MQ=255)
                                                                                gAGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCgg                                                                 <  1:3332165/148‑1 (MQ=255)
                                                                                 aGCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGt                                                                <  2:3102006/148‑1 (MQ=255)
                                                                                  gCTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGtt                                                               >  2:581989/1‑148 (MQ=255)
                                                                                   cTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTg                                                              <  2:28454/148‑1 (MQ=255)
                                                                                   cTGCGCGTAACCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTg                                                              >  2:26162/1‑148 (MQ=255)
                                                                                            aCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCg                                                     >  2:2212680/1‑148 (MQ=255)
                                                                                            aCCGTTGTTGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCg                                                     >  2:1503529/1‑148 (MQ=255)
                                                                                               gttgttGCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGa                                                  >  1:3279747/1‑148 (MQ=255)
                                                                                                ttgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGaa                                                 <  1:1069958/148‑1 (MQ=255)
                                                                                                ttgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGaa                                                 >  2:1459238/1‑148 (MQ=255)
                                                                                                 tgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAAt                                                >  1:1264988/1‑148 (MQ=255)
                                                                                                 tgttgCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAAt                                                >  1:1689836/1‑148 (MQ=255)
                                                                                                     gCGACAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACa                                            <  2:1931286/148‑1 (MQ=255)
                                                                                                         cAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgc                                        >  2:2485838/1‑148 (MQ=255)
                                                                                                         cAGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCACTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgc                                        >  2:3226575/1‑148 (MQ=255)
                                                                                                          aGGTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgcg                                       <  2:781679/148‑1 (MQ=255)
                                                                                                           ggTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATgcgc                                      <  1:2720651/148‑1 (MQ=255)
                                                                                                            gTATAGGCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGcgcc                                     >  1:1644222/1‑148 (MQ=255)
                                                                                                                 ggCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCaaa                                >  2:1710836/1‑148 (MQ=255)
                                                                                                                 ggCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCaaa                                >  1:591671/1‑148 (MQ=255)
                                                                                                                  gCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAAc                               >  2:533371/1‑148 (MQ=255)
                                                                                                                  gCATGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAAc                               >  2:1043416/1‑148 (MQ=255)
                                                                                                                     tGGACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGc                            <  2:591671/148‑1 (MQ=255)
                                                                                                                      ggACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCg                           <  1:2014317/148‑1 (MQ=255)
                                                                                                                      ggACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCg                           >  2:242465/1‑148 (MQ=255)
                                                                                                                       gACAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGa                          >  2:2693277/1‑148 (MQ=255)
                                                                                                                        aCAAACGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGaa                         >  2:1618290/1‑148 (MQ=255)
                                                                                                                           aaCGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAaga                      <  1:186772/148‑1 (MQ=255)
                                                                                                                            aCGTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAagag                     >  2:1831506/1‑148 (MQ=255)
                                                                                                                             cGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGGGc                    >  2:3340230/1‑148 (MQ=255)
                                                                                                                              gTCCTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGcc                   <  2:1351378/148‑1 (MQ=255)
                                                                                                                                ccTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCgg                 >  1:3333829/1‑148 (MQ=255)
                                                                                                                                ccTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCgg                 <  2:1922665/148‑1 (MQ=255)
                                                                                                                                ccTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCgg                 >  2:757290/1‑148 (MQ=255)
                                                                                                                                ccTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCgg                 >  2:3473527/1‑148 (MQ=255)
                                                                                                                                 cTGAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGa                >  2:1451480/1‑148 (MQ=255)
                                                                                                                                   gAAATCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGAtt              <  1:1152843/148‑1 (MQ=255)
                                                                                                                                     aaTCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTAt            <  1:3469252/148‑1 (MQ=255)
                                                                                                                                     aaTCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTAt            <  2:1240502/148‑1 (MQ=255)
                                                                                                                                      aTCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATc           <  2:1090994/148‑1 (MQ=255)
                                                                                                                                      aTCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATc           <  2:2540607/148‑1 (MQ=255)
                                                                                                                                       tCACTCTGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCt          >  2:791274/1‑148 (MQ=255)
                                                                                                                                          ctctGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGa       >  1:228453/1‑148 (MQ=255)
                                                                                                                                           tctGGTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGat      >  2:1298256/1‑148 (MQ=255)
                                                                                                                                              ggTTACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGATATc   <  1:1952613/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TAGGTAACACCATCCGTGCATTTGCTTCCGACAACGCGACTGTGGTTATCGGTACTTCTCTTGACCCGGATATGAATGACGAGCTGCGCGTAACCGTTGTTGCGACAGGTATCGGCATGGACAAACGTCCTGAAATCACTCTGGTGACCAATAAGCAGGTTCAGCAGCCAGTGATGGATCGCTACCAGCAGCATGGGATGGCTCCGCTGACCCAGGAGCAGAAGCCGGTTGCTAAAGTCGTGAATGACAATGCGCCGCAAACTGCGAAAGAGCCGGATTATCTGGATATCC  >  CP009273/102624‑102914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑