Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,101,777 G→T 6.8% E168* (GAA→TAA)  ymdB → O‑acetyl‑ADP‑ribose deacetylase; RNase III inhibitor during cold shock; putative cardiolipin synthase C regulatory subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,101,7770GT 6.8% 672.9 / 15.7 181E168* (GAA→TAA) ymdBO‑acetyl‑ADP‑ribose deacetylase; RNase III inhibitor during cold shock; putative cardiolipin synthase C regulatory subunit
Reads supporting (aligned to +/- strand):  ref base G (109/59);  new base T (8/5);  total (117/64)
Fisher's exact test for biased strand distribution p-value = 7.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

CCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCGTCTGGCC  >  CP009273/1101630‑1101924
                                                                                                                                                   |                                                                                                                                                   
ccTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACg                                                                                                                                                     >  1:1313821/1‑148 (MQ=255)
   gCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCATTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCTAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGaaa                                                                                                                                                  <  1:2525900/148‑1 (MQ=255)
   gCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGACAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTTTTTTTATGAAGAAAACGCCCACCTCTACGaaa                                                                                                                                                  <  1:2145413/148‑1 (MQ=255)
    cAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGAAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGATTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAg                                                                                                                                                 <  2:3152098/148‑1 (MQ=255)
      aTCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGAc                                                                                                                                               >  1:3276087/1‑148 (MQ=255)
       tCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACt                                                                                                                                              >  2:3486086/1‑148 (MQ=255)
           tACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCtt                                                                                                                                          >  2:2091886/1‑148 (MQ=255)
                 ggTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCcaa                                                                                                                                    >  2:1731720/1‑148 (MQ=255)
                         gTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGgaga                                                                                                                            <  2:1897507/148‑1 (MQ=255)
                          ttACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGACTTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCCACCAGGAGat                                                                                                                           >  2:1847535/1‑148 (MQ=255)
                          ttACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGat                                                                                                                           >  2:2419355/1‑148 (MQ=255)
                           tACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGatg                                                                                                                          >  1:2325771/1‑148 (MQ=255)
                           tACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGatg                                                                                                                          >  1:3192881/1‑148 (MQ=255)
                              ccTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGatgaat                                                                                                                       <  2:1470770/148‑1 (MQ=255)
                              ccTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGatgaat                                                                                                                       <  1:1090954/148‑1 (MQ=255)
                                tCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGatgaatga                                                                                                                     >  1:3120322/1‑148 (MQ=255)
                                tCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGatgaatga                                                                                                                     >  1:1839053/1‑148 (MQ=255)
                                tCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGatgaatga                                                                                                                     >  1:752807/1‑148 (MQ=255)
                                       gCAGCGGCTGAAATCGCAGTAAAACCCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAACACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGccc                                                                                                              <  1:1597980/148‑1 (MQ=255)
                                           cGGCTGAAAGCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGc                                                                                                          <  2:1846545/148‑1 (MQ=255)
                                             gCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTg                                                                                                        <  2:2513491/148‑1 (MQ=255)
                                              cTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTgg                                                                                                       <  1:2127427/148‑1 (MQ=255)
                                                 aaaTCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGa                                                                                                    >  1:507718/1‑148 (MQ=255)
                                                    tCGCAGTAAAAACCTTTTCATAATTTATTACCCGTCACTCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAgcg                                                                                                 <  2:1651862/148‑1 (MQ=255)
                                                        aGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGt                                                                                             <  1:2921469/148‑1 (MQ=255)
                                                         ggaaaaaCCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGtg                                                                                            >  1:2188094/3‑148 (MQ=255)
                                                           aaaaaCCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGtgct                                                                                          >  2:123733/1‑148 (MQ=255)
                                                           aaaaaCCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGtgct                                                                                          >  2:230628/1‑148 (MQ=255)
                                                           aaaaaCCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGtgct                                                                                          >  1:2830437/1‑148 (MQ=255)
                                                             aaaCCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGtgctgc                                                                                        <  2:3334332/148‑1 (MQ=255)
                                                              aaCCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGcc                                                                                       >  1:1296016/1‑148 (MQ=255)
                                                                 cGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACt                                                                                    >  2:2029562/1‑148 (MQ=255)
                                                                   tttCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACtgt                                                                                  >  2:3405839/1‑148 (MQ=255)
                                                                     tCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTgtgt                                                                                <  1:1061453/148‑1 (MQ=255)
                                                                         aaTTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGc                                                                            >  1:2873/1‑148 (MQ=255)
                                                                         aaTTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGc                                                                            >  1:2056340/1‑148 (MQ=255)
                                                                          aTTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCa                                                                           <  2:1802595/148‑1 (MQ=255)
                                                                          aTTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCa                                                                           >  1:2990135/1‑148 (MQ=255)
                                                                          aTTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCa                                                                           <  2:1423886/148‑1 (MQ=255)
                                                                          aTTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCa                                                                           >  2:645422/1‑148 (MQ=255)
                                                                               ttaCCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATc                                                                      >  2:3347927/1‑148 (MQ=255)
                                                                               ttaCCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATc                                                                      <  2:961652/148‑1 (MQ=255)
                                                                               ttaCCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATc                                                                      >  2:1419839/1‑148 (MQ=255)
                                                                                taCCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATcc                                                                     >  2:3238722/1‑148 (MQ=255)
                                                                                taCCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATcc                                                                     >  1:441718/1‑148 (MQ=255)
                                                                                taCCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATcc                                                                     >  1:589286/1‑148 (MQ=255)
                                                                                 aCCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATccc                                                                    >  2:2788323/1‑148 (MQ=255)
                                                                                  cccGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCg                                                                   <  2:666317/148‑1 (MQ=255)
                                                                                    cGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGt                                                                 >  1:3432176/1‑148 (MQ=255)
                                                                                      tCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCa                                                               <  2:1851664/148‑1 (MQ=255)
                                                                                       cACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAg                                                              >  2:400965/1‑148 (MQ=255)
                                                                                       cACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAg                                                              >  1:666259/1‑148 (MQ=255)
                                                                                            tttACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTgg                                                         >  2:797086/1‑148 (MQ=255)
                                                                                             ttACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGc                                                        >  1:1092429/1‑148 (MQ=255)
                                                                                             ttACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGc                                                        >  1:2235396/1‑148 (MQ=255)
                                                                                              tACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAGACTCCTTACCCAACCAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGcc                                                       <  2:1105114/148‑1 (MQ=255)
                                                                                              tACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGcc                                                       <  2:2635391/148‑1 (MQ=255)
                                                                                              tACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGcc                                                       <  2:2878309/148‑1 (MQ=255)
                                                                                              tACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGcc                                                       <  1:2710313/148‑1 (MQ=255)
                                                                                               aCCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCt                                                      >  2:337006/1‑148 (MQ=255)
                                                                                               aCCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCt                                                      <  2:2987431/148‑1 (MQ=255)
                                                                                                cccGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCtt                                                     >  1:1439137/1‑148 (MQ=255)
                                                                                                   gAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCttttt                                                  >  1:2984695/1‑148 (MQ=255)
                                                                                                    aaCAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTc                                                 >  1:3085024/1‑148 (MQ=255)
                                                                                                    aaCAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTc                                                 >  2:108517/1‑148 (MQ=255)
                                                                                                     aCAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTcc                                                >  1:1754893/1‑148 (MQ=255)
                                                                                                     aCAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTcc                                                <  2:3090464/148‑1 (MQ=255)
                                                                                                      cAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCct                                               >  1:1371811/1‑148 (MQ=255)
                                                                                                      cAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACTAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCct                                               >  1:2240363/1‑148 (MQ=255)
                                                                                                      cAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCct                                               >  2:2011391/1‑148 (MQ=255)
                                                                                                        ggTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCctct                                             <  2:774823/148‑1 (MQ=255)
                                                                                                            taCTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGgag                                         >  2:2743766/1‑148 (MQ=255)
                                                                                                             aCTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGgaga                                        <  2:1326404/148‑1 (MQ=255)
                                                                                                               tttGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGaaa                                      <  1:3363528/148‑1 (MQ=255)
                                                                                                                 tGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAg                                    >  1:717362/1‑148 (MQ=255)
                                                                                                                   tCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTc                                  >  1:1566787/1‑148 (MQ=255)
                                                                                                                      gTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTgg                               <  2:3092571/148‑1 (MQ=255)
                                                                                                                      gTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTgg                               <  2:1556666/148‑1 (MQ=255)
                                                                                                                      gTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTgg                               >  1:1213863/1‑148 (MQ=255)
                                                                                                                       ttATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGa                              >  2:720581/1‑148 (MQ=255)
                                                                                                                       ttATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGa                              <  2:3276087/148‑1 (MQ=255)
                                                                                                                       ttATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGa                              <  2:3348777/148‑1 (MQ=255)
                                                                                                                            atgaAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGt                         >  1:687168/1‑148 (MQ=255)
                                                                                                                            atgaAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGt                         <  2:460787/148‑1 (MQ=255)
                                                                                                                             tgaAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGtt                        >  1:1034792/1‑148 (MQ=255)
                                                                                                                             tgaAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGtt                        >  2:1293863/1‑148 (MQ=255)
                                                                                                                              gaagaaAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGttt                       >  1:3032495/1‑148 (MQ=255)
                                                                                                                              gaagaaAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGttt                       >  2:2693720/1‑148 (MQ=255)
                                                                                                                               aagaaAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGACAAGTCTGGATGCGTTTg                      >  2:3363871/1‑148 (MQ=255)
                                                                                                                                agaaAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTgc                     >  1:109451/1‑148 (MQ=255)
                                                                                                                                    aaCGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTgccgcc                 >  2:1280178/1‑148 (MQ=255)
                                                                                                                                        cccACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGt             <  2:666259/148‑1 (MQ=255)
                                                                                                                                          cACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTAt           >  2:2126304/1‑148 (MQ=255)
                                                                                                                                            ccTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCg         >  1:3175986/1‑148 (MQ=255)
                                                                                                                                            ccTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCg         >  1:1406529/1‑148 (MQ=255)
                                                                                                                                            ccTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCg         >  2:1075008/1‑148 (MQ=255)
                                                                                                                                            ccTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTATCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCg         >  2:2571850/1‑148 (MQ=255)
                                                                                                                                              tctACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCGTc       <  1:2577303/148‑1 (MQ=255)
                                                                                                                                                  cGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCGTCTGGc   >  1:163388/1‑148 (MQ=255)
                                                                                                                                                   gAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCGTCTGGcc  <  2:2159242/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTGGCCTTTTTCCTCTGGAGAAAAGTCTGGATGCGTTTGCCGCCCGGTATCGTCTGGCC  >  CP009273/1101630‑1101924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑