Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,224,292 G→A 100% D8N (GAT→AAT)  ycgN → UPF0153 family cysteine cluster protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,224,2920GA100.0% 767.0 / ‑6.7 234D8N (GAT→AAT) ycgNUPF0153 family cysteine cluster protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (100/133);  minor base T (0/1);  total (100/134)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

CTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTC  >  CP009273/1224147‑1224436
                                                                                                                                                 |                                                                                                                                                
cTGACTGTCACTTTCGATGGGCATTCTTTGACAACTCGCGATTTGTAATACTGTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAAt                                                                                                                                                <  2:2681890/148‑1 (MQ=255)
cTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAAt                                                                                                                                                >  2:1271278/1‑148 (MQ=255)
   aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTa                                                                                                                                             <  1:587874/148‑1 (MQ=255)
   aCAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGGGCGTTTTCACGTAATGGCGGAACACCTGATGAGCACTGTa                                                                                                                                             >  2:1905633/1‑148 (MQ=255)
    cAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTAc                                                                                                                                            >  2:1715474/1‑148 (MQ=255)
        cACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCttt                                                                                                                                        <  2:2827169/148‑1 (MQ=255)
         aCTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTc                                                                                                                                       <  1:2951989/148‑1 (MQ=255)
          cTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCt                                                                                                                                      >  2:3043907/1‑148 (MQ=255)
            ttCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTgg                                                                                                                                    <  2:1598196/148‑1 (MQ=255)
             tCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGc                                                                                                                                   <  2:36248/148‑1 (MQ=255)
             tCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGc                                                                                                                                   <  2:37939/148‑1 (MQ=255)
               gATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCaa                                                                                                                                 <  1:875967/148‑1 (MQ=255)
                aTGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCaaa                                                                                                                                <  2:1592684/148‑1 (MQ=255)
                  gggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGt                                                                                                                              >  1:3284439/1‑148 (MQ=255)
                  gggCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGt                                                                                                                              >  2:652607/1‑148 (MQ=255)
                    gCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaa                                                                                                                            >  1:3543748/1‑148 (MQ=255)
                     cATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTaaa                                                                                                                           <  1:209015/148‑1 (MQ=255)
                        tCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAcc                                                                                                                        >  2:682955/1‑148 (MQ=255)
                        tCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAcc                                                                                                                        <  2:2885379/148‑1 (MQ=255)
                        tCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAcc                                                                                                                        >  2:3063143/1‑148 (MQ=255)
                         cTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAccc                                                                                                                       <  1:47570/148‑1 (MQ=255)
                         cTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAAccc                                                                                                                       >  1:713241/1‑148 (MQ=255)
                           ttGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTg                                                                                                                     >  2:2478927/1‑148 (MQ=255)
                            tGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTgg                                                                                                                    <  1:1314558/148‑1 (MQ=255)
                              aCAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAc                                                                                                                  >  2:62106/1‑148 (MQ=255)
                                  cTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGaaa                                                                                                              >  2:2290634/1‑148 (MQ=255)
                                  cTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGaaa                                                                                                              <  2:1692686/148‑1 (MQ=255)
                                    cgcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATg                                                                                                            <  2:3087029/148‑1 (MQ=255)
                                     gcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAACTGa                                                                                                           >  2:1508754/1‑148 (MQ=255)
                                     gcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGa                                                                                                           <  2:1925493/148‑1 (MQ=255)
                                     gcgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGa                                                                                                           <  2:2415697/148‑1 (MQ=255)
                                      cgTTTTGTAATACTTTTTGCCGCCTGAAGGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAg                                                                                                          <  2:1865581/148‑1 (MQ=255)
                                      cgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTACAACCCTGGACGAAATGAg                                                                                                          >  2:2647880/1‑148 (MQ=255)
                                      cgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAg                                                                                                          >  2:1169057/1‑148 (MQ=255)
                                      cgTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAg                                                                                                          >  2:2941815/1‑148 (MQ=255)
                                       gTTTTTTAATTGTTTTTGCCGCCTGAAAGCGGCGGAAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCTATGTACCTTTATGGCAAAGTAAAACCCTGGACGAAATGAGc                                                                                                         <  1:2144783/148‑1 (MQ=255)
                                        ttttGTAATACTTTTTGCCGCCTGAAAGCGGCGGCACAACTTGCATCGCTGTGCCAGACTGGTTCTAAGGTGCGTTTTAACGTACTGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCg                                                                                                        >  2:110466/1‑148 (MQ=255)
                                        ttttGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCg                                                                                                        >  2:110439/1‑148 (MQ=255)
                                        ttttGTAATACTTTTTGCCGCATGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCg                                                                                                        <  1:480755/148‑1 (MQ=255)
                                        ttttGTAATACTTTTTGCCGCATGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCg                                                                                                        <  1:781008/148‑1 (MQ=255)
                                         tttGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGa                                                                                                       <  2:890776/148‑1 (MQ=255)
                                           tGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATg                                                                                                     <  2:798674/148‑1 (MQ=255)
                                               aTACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGa                                                                                                 >  1:37640/1‑148 (MQ=255)
                                               aTACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGa                                                                                                 >  1:3350872/1‑148 (MQ=255)
                                                tACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGaa                                                                                                >  1:1145377/1‑148 (MQ=255)
                                                tACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGaa                                                                                                >  1:2917851/1‑148 (MQ=255)
                                                         ccgccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCg                                                                                       <  2:3131603/148‑1 (MQ=255)
                                                          cgccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATATGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGt                                                                                      <  2:205037/148‑1 (MQ=255)
                                                           gccTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGtt                                                                                     >  1:194017/1‑148 (MQ=255)
                                                                       cggcAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTg                                                                         <  1:563028/148‑1 (MQ=255)
                                                                             aaCTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCa                                                                   <  2:3170631/148‑1 (MQ=255)
                                                                             aaCTTGCATCGCTGTGCCAGACTGGGTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGAGGTCa                                                                   >  1:2299487/1‑148 (MQ=255)
                                                                               cTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAgt                                                                 >  1:1102931/1‑148 (MQ=255)
                                                                               cTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAgt                                                                 >  1:2615333/1‑148 (MQ=255)
                                                                                ttGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATTAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGAAGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAgtg                                                                <  2:432225/148‑1 (MQ=255)
                                                                                ttGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAgtg                                                                <  2:323967/148‑1 (MQ=255)
                                                                                    aTCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGc                                                            >  2:2475253/1‑148 (MQ=255)
                                                                                     tCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGcc                                                           <  2:583869/148‑1 (MQ=255)
                                                                                     tCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGcc                                                           >  2:2602694/1‑148 (MQ=255)
                                                                                      cGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCt                                                          >  2:2531267/1‑148 (MQ=255)
                                                                                      cGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCt                                                          >  1:1017767/1‑148 (MQ=255)
                                                                                        cTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGc                                                        <  1:3102907/148‑1 (MQ=255)
                                                                                           tgCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATa                                                     >  2:474654/1‑148 (MQ=255)
                                                                                             ccAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACTAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATaaa                                                   <  2:1383170/148‑1 (MQ=255)
                                                                                               aGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACt                                                 >  1:2188863/1‑148 (MQ=255)
                                                                                               aGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGTTGGTTGCGGGCAGGGTTGCCTGCATaaaat                                                 >  1:1515846/1‑146 (MQ=255)
                                                                                                  cTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgat                                              <  1:314866/148‑1 (MQ=255)
                                                                                                     gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatgga                                           <  2:1779260/148‑1 (MQ=255)
                                                                                                     gTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatgga                                           <  2:26151/148‑1 (MQ=255)
                                                                                                      ttATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTtatggat                                          >  2:1007137/1‑148 (MQ=255)
                                                                                                      ttATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTgatggat                                          >  2:3328893/1‑148 (MQ=255)
                                                                                                        ataAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGa                                        <  1:2522787/148‑1 (MQ=255)
                                                                                                          aaGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAg                                      <  2:2368056/148‑1 (MQ=255)
                                                                                                          aaGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAg                                      <  2:1652209/148‑1 (MQ=255)
                                                                                                            ggTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGac                                    <  1:2767283/148‑1 (MQ=255)
                                                                                                             gTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGaca                                   >  2:464213/1‑148 (MQ=255)
                                                                                                             gTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGTTGGTTTGGGTCAGTGTTGGCTGCATAAACTGATGGTTGAAGaca                                   >  1:91798/1‑148 (MQ=255)
                                                                                                                   tttAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACcgacg                             >  1:1182996/1‑148 (MQ=255)
                                                                                                                   tttAACGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACcgacg                             >  2:821986/1‑148 (MQ=255)
                                                                                                                        cGTAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATc                        <  2:1141200/148‑1 (MQ=255)
                                                                                                                          tAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTa                      <  1:2794564/148‑1 (MQ=255)
                                                                                                                          tAATGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTa                      <  2:717281/148‑1 (MQ=255)
                                                                                                                           aaTGGCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTAc                     >  1:1654789/1‑148 (MQ=255)
                                                                                                                               gCGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCa                 <  1:110439/148‑1 (MQ=255)
                                                                                                                                cGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCAc                <  1:2435805/148‑1 (MQ=255)
                                                                                                                                cGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCAc                <  2:2123297/148‑1 (MQ=255)
                                                                                                                                cGGAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCAc                <  1:652607/148‑1 (MQ=255)
                                                                                                                                  gAACACCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATTGTTTGGGTAAGTGTTGCCAGCATAAAATGATGGATTAATACAACGAACTAATCAAATTCAcaa              >  1:3337683/1‑146 (MQ=255)
                                                                                                                                    acacCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAAc            <  2:2933748/148‑1 (MQ=255)
                                                                                                                                     cacCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACg           >  1:1873406/1‑148 (MQ=255)
                                                                                                                                      acCTTATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGt          >  2:3325336/1‑148 (MQ=255)
                                                                                                                                      acCTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGCTGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACCAAATCTACTTCACTAACGt          >  2:2096650/1‑148 (MQ=255)
                                                                                                                                       ccTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTc         >  2:1068713/1‑148 (MQ=255)
                                                                                                                                        cTGATGAGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCg        >  2:277561/1‑148 (MQ=255)
                                                                                                                                         tgatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGc       <  1:2471112/148‑1 (MQ=255)
                                                                                                                                         tgatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTGGTGTGATGGTTGGGGTCAGTGTTGCCTGCATAAACTGATGGATTAAGAAACCGACGAAATATACTTCACTAAAGTCGc       >  1:1610636/1‑148 (MQ=255)
                                                                                                                                          gatgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGcc      <  2:3454535/148‑1 (MQ=255)
                                                                                                                                            tgaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGTTGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTg    >  1:3177618/1‑148 (MQ=255)
                                                                                                                                             gaGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGt   >  2:3289258/1‑148 (MQ=255)
                                                                                                                                              aGCAATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTc  >  1:1939311/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTGACAGTCACTTTCGATGGGCATTCTTTGACAACTCGCGTTTTGTAATACTTTTTGCCGCCTGAAAGCGGCGGCAAAACTTGCATCGCTGTGCCAGACTGGTTATAAGGTGCGTTTTAACGTAATGGCGGAACACCTGATGAGCGATGTACCTTTCTGGCAAAGTAAAACCCTGGACGAAATGAGCGATGCGGAATGGGAGTCGTTGTGTGATGGTTGCGGTCAGTGTTGCCTGCATAAACTGATGGATGAAGACACCGACGAAATCTACTTCACTAACGTCGCCTGTC  >  CP009273/1224147‑1224436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑