Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 12,901 G→A 100% D247N (GAT→AAT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927312,9010GA100.0% 727.9 / ‑6.7 218D247N (GAT→AAT) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (125/91);  minor base T (0/2);  total (125/93)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 223 total aligned reads displayed.

TACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAAC  >  CP009273/12756‑13045
                                                                                                                                                 |                                                                                                                                                
tACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCGGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAAt                                                                                                                                                <  2:1731085/148‑1 (MQ=255)
  cTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCa                                                                                                                                              >  2:2374318/1‑148 (MQ=255)
    ttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAgg                                                                                                                                            >  1:2260418/1‑148 (MQ=255)
      cGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGc                                                                                                                                          <  2:1104615/148‑1 (MQ=255)
       gATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCa                                                                                                                                         <  2:2824832/148‑1 (MQ=255)
        atttttCTATTTTCGAAATCGACGAAGTTGACGGCTAAAAAACCTTCGAAGTTCTGGCAAACAACGGTGATACCCAGCTGGGGGTTGAAGACTTCGACAGCCTTCTTATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAt                                                                                                                                        <  1:1044894/145‑1 (MQ=255)
         tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       >  1:871670/1‑148 (MQ=255)
         tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       >  1:1504829/1‑148 (MQ=255)
         tatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAtt                                                                                                                                       >  1:580963/1‑148 (MQ=255)
            ttCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAc                                                                                                                                    <  2:1236159/148‑1 (MQ=255)
            ttCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAc                                                                                                                                    <  2:1284421/148‑1 (MQ=255)
            ttCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAc                                                                                                                                    >  1:2681458/1‑148 (MQ=255)
            ttCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAc                                                                                                                                    >  2:510982/1‑148 (MQ=255)
                attatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTgc                                                                                                                                >  2:463818/1‑148 (MQ=255)
                   atCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCa                                                                                                                             >  2:3310591/1‑148 (MQ=255)
                      gAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACg                                                                                                                          <  2:550912/148‑1 (MQ=255)
                      gAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACg                                                                                                                          <  2:550914/148‑1 (MQ=255)
                       aaaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGa                                                                                                                         <  1:2633450/148‑1 (MQ=255)
                       aaaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGa                                                                                                                         >  1:710850/1‑148 (MQ=255)
                         aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATc                                                                                                                       >  1:932305/1‑148 (MQ=255)
                           cgacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCg                                                                                                                     >  2:3533164/1‑148 (MQ=255)
                            gacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGc                                                                                                                    >  2:3221308/1‑148 (MQ=255)
                              cgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTg                                                                                                                  >  2:630577/1‑148 (MQ=255)
                                 aGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCa                                                                                                               <  1:2336994/148‑1 (MQ=255)
                                  gTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCaa                                                                                                              >  1:173250/1‑148 (MQ=255)
                                      aCGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCa                                                                                                          >  1:1603557/1‑148 (MQ=255)
                                          cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc                                                                                                      <  2:3116966/148‑1 (MQ=255)
                                               aaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaa                                                                                                 <  1:3152509/148‑1 (MQ=255)
                                                 aCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaag                                                                                               >  2:1395654/1‑148 (MQ=255)
                                                 aCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaag                                                                                               >  2:1566773/1‑148 (MQ=255)
                                                 aCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaag                                                                                               >  1:1624852/1‑148 (MQ=255)
                                                  ccTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaaga                                                                                              >  1:1626198/1‑148 (MQ=255)
                                                   cTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaa                                                                                             >  2:116046/1‑148 (MQ=255)
                                                    ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaag                                                                                            <  2:1674564/148‑1 (MQ=255)
                                                           ttCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGa                                                                                     >  1:2118854/1‑148 (MQ=255)
                                                            tCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaa                                                                                    <  1:1381442/148‑1 (MQ=255)
                                                                gCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAg                                                                                <  2:3464026/148‑1 (MQ=255)
                                                                gCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAg                                                                                >  1:602574/1‑148 (MQ=255)
                                                                  aaccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCg                                                                              <  1:1916327/148‑1 (MQ=255)
                                                                  aaccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCg                                                                              <  1:837307/148‑1 (MQ=255)
                                                                   accaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGa                                                                             >  1:1095409/1‑148 (MQ=255)
                                                                   accaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGa                                                                             <  2:739854/148‑1 (MQ=255)
                                                                    ccgacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaa                                                                            >  2:3344022/4‑148 (MQ=255)
                                                                      aacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaaa                                                                          <  1:1816513/148‑1 (MQ=255)
                                                                       acGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAAt                                                                         <  1:2537492/148‑1 (MQ=255)
                                                                         ggTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCg                                                                       >  2:1927384/1‑148 (MQ=255)
                                                                          gTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGa                                                                      <  1:3101786/148‑1 (MQ=255)
                                                                            gATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAAc                                                                    <  2:2861985/148‑1 (MQ=255)
                                                                            gATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAAc                                                                    <  2:580963/148‑1 (MQ=255)
                                                                                cccACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTc                                                                >  2:495532/1‑148 (MQ=255)
                                                                                 ccACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCt                                                               >  1:1460800/1‑148 (MQ=255)
                                                                                 ccACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCt                                                               >  1:2836182/1‑148 (MQ=255)
                                                                                  cACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCtt                                                              <  1:1251473/148‑1 (MQ=255)
                                                                                  cACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCtt                                                              <  2:1667877/148‑1 (MQ=255)
                                                                                     cTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCg                                                           >  1:1210/1‑148 (MQ=255)
                                                                                      gggggggTGAAGACTTCGACAGCCGTCTGATCAACTATCGGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGACGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGc                                                          <  1:1846334/147‑1 (MQ=255)
                                                                                       ggggggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCt                                                         <  2:778244/148‑1 (MQ=255)
                                                                                       ggggggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCt                                                         <  2:1855925/148‑1 (MQ=255)
                                                                                        gggggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTc                                                        >  1:2155506/1‑148 (MQ=255)
                                                                                        gggggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTc                                                        <  2:2468767/148‑1 (MQ=255)
                                                                                             tGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTcagcag                                                   <  1:94985/148‑1 (MQ=255)
                                                                                              gAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGa                                                  >  1:2779310/1‑148 (MQ=255)
                                                                                              gAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGa                                                  >  1:1007528/1‑148 (MQ=255)
                                                                                                aGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGAcc                                                >  2:3052573/1‑148 (MQ=255)
                                                                                                 gACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCg                                               <  1:2374318/148‑1 (MQ=255)
                                                                                                 gACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCg                                               <  1:143817/148‑1 (MQ=255)
                                                                                                    ttCTACAGCAGTCTGATTATATATCTGTTTGAATATTTATATAATTATAAGGGATTTGACATGCGAAACTATCCGATGGATATTCAGGGACTGATAGAAGCGGCTGAAATAGCGAAAATCGATCTGTCTTCCGCTCAGCAGACCGACg                                            <  1:427794/148‑1 (MQ=255)
                                                                                                     tCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGt                                           <  2:932305/148‑1 (MQ=255)
                                                                                                     tCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGt                                           >  2:2588897/1‑148 (MQ=255)
                                                                                                       gACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTa                                         >  1:3413253/1‑148 (MQ=255)
                                                                                                        aCAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTaa                                        >  1:1333194/1‑148 (MQ=255)
                                                                                                        aCAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTaa                                        >  2:139260/1‑148 (MQ=255)
                                                                                                          aGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAAcc                                      >  1:2600302/1‑148 (MQ=255)
                                                                                                            ccGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTg                                    >  1:3006317/1‑148 (MQ=255)
                                                                                                            ccGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTg                                    >  2:865376/1‑148 (MQ=255)
                                                                                                             cGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGc                                   <  2:674523/148‑1 (MQ=255)
                                                                                                               tCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCa                                 >  1:692549/1‑148 (MQ=255)
                                                                                                                cTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCAt                                <  2:1800603/148‑1 (MQ=255)
                                                                                                                  gATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATAc                              >  2:1770434/1‑148 (MQ=255)
                                                                                                                   aTCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACa                             >  2:2778351/1‑148 (MQ=255)
                                                                                                                    tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACAt                            <  1:2145670/148‑1 (MQ=255)
                                                                                                                    tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACAt                            >  2:1222403/1‑148 (MQ=255)
                                                                                                                     cAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCCTACATc                           >  2:2046985/1‑148 (MQ=255)
                                                                                                                     cAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATc                           >  1:756605/1‑148 (MQ=255)
                                                                                                                     cAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATc                           >  1:286188/1‑148 (MQ=255)
                                                                                                                      aaCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCa                          >  2:1017565/1‑148 (MQ=255)
                                                                                                                       aCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                         >  1:1358741/1‑148 (MQ=255)
                                                                                                                       aCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                         >  2:1513129/1‑148 (MQ=255)
                                                                                                                         tATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTg                       <  2:710850/148‑1 (MQ=255)
                                                                                                                           tCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCa                     >  2:2976049/1‑148 (MQ=255)
                                                                                                                           tCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCa                     <  2:946294/148‑1 (MQ=255)
                                                                                                                           tCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCa                     >  2:2217560/1‑148 (MQ=255)
                                                                                                                               gTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcg                 >  2:2896552/1‑148 (MQ=255)
                                                                                                                                ttGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgc                >  2:2720773/1‑148 (MQ=255)
                                                                                                                                 tGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgcg               >  2:1174534/1‑148 (MQ=255)
                                                                                                                                 tGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgcg               >  2:2667243/1‑148 (MQ=255)
                                                                                                                                  gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGa              <  1:2470800/148‑1 (MQ=255)
                                                                                                                                  gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGa              <  1:2499755/148‑1 (MQ=255)
                                                                                                                                     gaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCg           >  1:2415130/1‑148 (MQ=255)
                                                                                                                                              aaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAac  >  2:880858/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAAC  >  CP009273/12756‑13045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑