Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,249,227 G→T 100% R728S (CGT→AGT)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,249,2270GT96.0% 522.0 / 9.8 174R728S (CGT→AGT) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (6/2);  new base T (91/75);  total (97/77)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 185 total aligned reads displayed.

CTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTT  >  CP009273/1249082‑1249370
                                                                                                                                                 |                                                                                                                                               
cTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACttt                                                                                                                                               <  1:756949/148‑1 (MQ=255)
cTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACttt                                                                                                                                               <  2:3178002/148‑1 (MQ=255)
cTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACttt                                                                                                                                               <  1:1606447/148‑1 (MQ=255)
cTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACttt                                                                                                                                               <  1:1606626/148‑1 (MQ=255)
cTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACttt                                                                                                                                               <  1:2620458/148‑1 (MQ=255)
cTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACttt                                                                                                                                               <  2:144197/148‑1 (MQ=255)
 tGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACtttt                                                                                                                                              <  2:3105681/148‑1 (MQ=255)
  gTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTa                                                                                                                                             >  1:1184842/1‑148 (MQ=255)
   tAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTAt                                                                                                                                            >  2:881308/1‑148 (MQ=255)
    aaaaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATc                                                                                                                                           <  1:2978077/148‑1 (MQ=255)
      aaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATccc                                                                                                                                         >  2:1677618/1‑148 (MQ=255)
      aaaCCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATccc                                                                                                                                         >  2:1677569/1‑148 (MQ=255)
         ccGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCccac                                                                                                                                      >  2:767375/1‑148 (MQ=255)
           gTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCccacca                                                                                                                                    <  1:3529780/148‑1 (MQ=255)
             tttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCaaa                                                                                                                                  >  2:2140476/1‑148 (MQ=255)
             tttGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCaaa                                                                                                                                  >  2:2140438/1‑148 (MQ=255)
                gtgCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTg                                                                                                                               <  2:942364/148‑1 (MQ=255)
                 tgCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTgg                                                                                                                              <  1:1241847/148‑1 (MQ=255)
                  gCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGa                                                                                                                             <  2:585527/148‑1 (MQ=255)
                  gCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGa                                                                                                                             <  2:2407892/148‑1 (MQ=255)
                  gCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGa                                                                                                                             <  1:1779466/148‑1 (MQ=255)
                   ccATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGAt                                                                                                                            <  1:1329583/148‑1 (MQ=255)
                    cATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATa                                                                                                                           <  2:2383285/148‑1 (MQ=255)
                    cATGTAACTGGCGTACAGTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATa                                                                                                                           <  2:2384270/148‑1 (MQ=255)
                     aTGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATAc                                                                                                                          >  2:356608/1‑148 (MQ=255)
                     aTGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATAc                                                                                                                          >  2:3463507/1‑148 (MQ=255)
                     aTGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATAc                                                                                                                          >  2:1777872/1‑148 (MQ=255)
                          aCTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTg                                                                                                                     >  2:1225151/1‑148 (MQ=255)
                          aCTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTg                                                                                                                     <  2:1012420/148‑1 (MQ=255)
                           cTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGt                                                                                                                    >  1:1470924/1‑148 (MQ=255)
                            tGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTa                                                                                                                   >  1:2183835/1‑148 (MQ=255)
                             ggCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTaa                                                                                                                  <  2:2914992/148‑1 (MQ=255)
                              gCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAAc                                                                                                                 >  2:2137278/1‑148 (MQ=255)
                               cGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCTACCAAACTGGATACCGCTGTAAcc                                                                                                                <  2:2530914/148‑1 (MQ=255)
                               cGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAAcc                                                                                                                <  1:1708819/148‑1 (MQ=255)
                                      tCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCa                                                                                                         <  2:278499/148‑1 (MQ=255)
                                         cATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAg                                                                                                      <  2:1262384/148‑1 (MQ=255)
                                             tAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGc                                                                                                  >  1:2897581/1‑148 (MQ=255)
                                                tCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCa                                                                                               <  1:70951/148‑1 (MQ=255)
                                                  tGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGt                                                                                             <  2:2578625/148‑1 (MQ=255)
                                                   gAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGtt                                                                                            >  1:1261750/1‑148 (MQ=255)
                                                     aCGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTg                                                                                          <  1:1869670/148‑1 (MQ=255)
                                                     aCGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTg                                                                                          >  2:1281912/1‑148 (MQ=255)
                                                             tACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGc                                                                                  >  2:1826825/1‑148 (MQ=255)
                                                             tACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGc                                                                                  >  2:78505/1‑148 (MQ=255)
                                                             tACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGc                                                                                  >  1:3443710/1‑148 (MQ=255)
                                                             tACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGc                                                                                  >  1:278113/1‑148 (MQ=255)
                                                                cGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCaaa                                                                               >  1:1872205/1‑148 (MQ=255)
                                                                  tCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAgg                                                                             <  2:136781/148‑1 (MQ=255)
                                                                      cTCCGCTATAGTCCGGCGATGCATGTGTTGAGCAAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCTCTGTAACCAATGTAAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTc                                                                         <  1:2604633/148‑1 (MQ=255)
                                                                        ccGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCa                                                                       >  1:426046/1‑148 (MQ=255)
                                                                             ataGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCtt                                                                  >  2:226734/1‑148 (MQ=255)
                                                                             ataGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCtt                                                                  >  2:2021505/1‑148 (MQ=255)
                                                                              taGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTc                                                                 <  2:1087231/148‑1 (MQ=255)
                                                                              taGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTc                                                                 >  1:2195032/1‑148 (MQ=255)
                                                                                   cGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCa                                                            >  2:1350896/1‑148 (MQ=255)
                                                                                   cGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCa                                                            <  1:2137349/148‑1 (MQ=255)
                                                                                     gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          <  1:2003016/148‑1 (MQ=255)
                                                                                     gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          >  2:1555957/1‑148 (MQ=255)
                                                                                     gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          >  1:982574/1‑148 (MQ=255)
                                                                                     gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          <  2:459268/148‑1 (MQ=255)
                                                                                     gCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATa                                                          >  1:3404457/1‑148 (MQ=255)
                                                                                     gCGATACATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCACCCATa                                                          >  2:2698310/1‑148 (MQ=255)
                                                                                       gATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAAc                                                        <  2:979599/148‑1 (MQ=255)
                                                                                         tGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTg                                                      >  2:566719/1‑148 (MQ=255)
                                                                                          gCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgc                                                     >  1:2705671/1‑148 (MQ=255)
                                                                                           cATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgcg                                                    <  1:2202741/148‑1 (MQ=255)
                                                                                           cATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTgcg                                                    <  2:26949/148‑1 (MQ=255)
                                                                                             tgtgTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCa                                                  >  1:2291002/1‑148 (MQ=255)
                                                                                               tgtTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATACACACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTAGACACAGGCAAACGAGTCCAGGCTTCCCCCATAACTGCGCAAc                                                >  1:2851952/1‑148 (MQ=255)
                                                                                                gtTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTTACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAAcc                                               <  1:2467072/148‑1 (MQ=255)
                                                                                                 ttGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCa                                              >  1:316486/1‑148 (MQ=255)
                                                                                                 ttGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCa                                              >  1:316516/1‑148 (MQ=255)
                                                                                                      ccAATATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGatat                                         >  2:917688/1‑148 (MQ=255)
                                                                                                        aaTATACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTa                                       >  2:1404480/1‑148 (MQ=255)
                                                                                                         atatACAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTAc                                      <  1:965776/148‑1 (MQ=255)
                                                                                                           ataCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCg                                    >  1:2096562/1‑148 (MQ=255)
                                                                                                            taCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGt                                   <  1:309522/148‑1 (MQ=255)
                                                                                                            taCAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGt                                   >  2:136909/1‑148 (MQ=255)
                                                                                                              cAGACCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCt                                 <  2:1217369/148‑1 (MQ=255)
                                                                                                                 aCCGACATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTa                              >  2:2478480/1‑148 (MQ=255)
                                                                                                                     aCATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActct                          <  1:2363503/148‑1 (MQ=255)
                                                                                                                     aCATACAACGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTActct                          >  2:1891864/1‑148 (MQ=255)
                                                                                                                            aCGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCg                   >  1:2819836/1‑148 (MQ=255)
                                                                                                                            aCGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCg                   <  2:516960/148‑1 (MQ=255)
                                                                                                                            aCGGCATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCg                   >  2:1591204/1‑148 (MQ=255)
                                                                                                                                cATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAgg               <  2:1184842/148‑1 (MQ=255)
                                                                                                                                cATTACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAgg               <  2:1431792/148‑1 (MQ=255)
                                                                                                                                  ttACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTc             <  1:3237166/148‑1 (MQ=255)
                                                                                                                                   tACATCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCa            >  2:3130289/1‑148 (MQ=255)
                                                                                                                                       tCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCa        >  1:1400955/1‑148 (MQ=255)
                                                                                                                                       tCAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCa        >  1:1400966/1‑148 (MQ=255)
                                                                                                                                        cAGAGAGACTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCAt       >  2:2931695/1‑148 (MQ=255)
                                                                                                                                          gagagaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATcc     >  2:2971511/1‑148 (MQ=255)
                                                                                                                                           agagaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCg    >  2:186554/1‑148 (MQ=255)
                                                                                                                                           agagaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCg    >  1:1043020/1‑148 (MQ=255)
                                                                                                                                           agagaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCg    >  1:1042691/1‑148 (MQ=255)
                                                                                                                                            gagaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGt   <  2:404443/148‑1 (MQ=255)
                                                                                                                                            gagaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGt   <  1:755539/148‑1 (MQ=255)
                                                                                                                                             agaCTTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGtt  >  2:743357/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGTAAAAACCGTTTTGTGCCATGTAACTGGCGTACATTCCCATGTAGTCTGAACGTGCGGTACCGTCGCCTCCGCTATAGTCCGGCGATGCATGTGTTGAGCCAATATACAGACCGACATACAACGGCATTACATCAGAGAGACGTTTATCCCCACCAAACTGGATACCGCTGTAACCCATGTCAAAGCCGCTCAGTTTGCCACTGGCAAAGGAGTCCAGGCTTCCCCCATAACTGCGCAACCAGATATTACCGTCTTTACTCTGATTTCGCAGGTCACCCATCCGTT  >  CP009273/1249082‑1249370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑