Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,249,716 G→T 7.1% R565S (CGC→AGC)  ycgV ← putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,249,7160GT 7.1% 692.7 / 18.8 195R565S (CGC→AGC) ycgVputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (85/93);  new base T (9/5);  total (94/101)
Fisher's exact test for biased strand distribution p-value = 2.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 198 total aligned reads displayed.

CTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAA  >  CP009273/1249570‑1249863
                                                                                                                                                  |                                                                                                                                                   
cTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCgg                                                                                                                                                    <  1:1788566/148‑1 (MQ=255)
  cccAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGAt                                                                                                                                                  <  2:3105866/148‑1 (MQ=255)
  cccAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGAt                                                                                                                                                  >  1:2849530/1‑148 (MQ=255)
     aGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGc                                                                                                                                               >  2:2293340/1‑148 (MQ=255)
       ttAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCa                                                                                                                                             >  1:1209856/1‑148 (MQ=255)
        tAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCaa                                                                                                                                            <  2:187002/148‑1 (MQ=255)
            gCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACg                                                                                                                                        <  1:841161/148‑1 (MQ=255)
              cATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAACACGTg                                                                                                                                      <  1:2295623/148‑1 (MQ=255)
               aTTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGa                                                                                                                                     <  1:1113608/148‑1 (MQ=255)
                tttttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGAt                                                                                                                                    >  2:2554209/1‑148 (MQ=255)
                  tttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTa                                                                                                                                  <  1:496985/148‑1 (MQ=255)
                   ttACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTAc                                                                                                                                 <  1:3261916/148‑1 (MQ=255)
                        cacaTCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCa                                                                                                                            <  1:3501708/148‑1 (MQ=255)
                             cGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCactact                                                                                                                       <  2:173172/148‑1 (MQ=255)
                             cGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCactact                                                                                                                       <  2:242327/148‑1 (MQ=255)
                                 cAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCg                                                                                                                   <  2:2967130/148‑1 (MQ=255)
                                 cAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCg                                                                                                                   <  2:3419369/148‑1 (MQ=255)
                                   gATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCt                                                                                                                 >  2:18973/1‑148 (MQ=255)
                                     tatCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGa                                                                                                               <  2:1284490/148‑1 (MQ=255)
                                      atCCCCCCACCTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGat                                                                                                              <  2:2497542/148‑1 (MQ=255)
                                      atCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGat                                                                                                              <  1:2889423/148‑1 (MQ=255)
                                      atCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGat                                                                                                              <  2:1237678/148‑1 (MQ=255)
                                       tCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGata                                                                                                             >  1:999960/1‑148 (MQ=255)
                                         cccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATAtt                                                                                                           <  1:6681/148‑1 (MQ=255)
                                         cccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATAtt                                                                                                           <  1:1634595/148‑1 (MQ=255)
                                         cccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATAtt                                                                                                           <  1:1634464/148‑1 (MQ=255)
                                          ccccAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTc                                                                                                          >  2:2719999/1‑148 (MQ=255)
                                             cAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAAt                                                                                                       >  1:1165515/1‑148 (MQ=255)
                                               aCTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATaa                                                                                                     <  1:1018298/148‑1 (MQ=255)
                                                  cAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATc                                                                                                  <  1:1855395/148‑1 (MQ=255)
                                                    aCCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATccc                                                                                                >  2:2677833/1‑148 (MQ=255)
                                                    aCCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATccc                                                                                                >  1:2402520/1‑148 (MQ=255)
                                                      cTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCt                                                                                              <  1:1874118/148‑1 (MQ=255)
                                                      cTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCt                                                                                              <  2:2958598/148‑1 (MQ=255)
                                                       tGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCtt                                                                                             <  1:712009/148‑1 (MQ=255)
                                                         agaagaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTtta                                                                                           <  2:671926/148‑1 (MQ=255)
                                                           aagaCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTttatt                                                                                         <  2:1044893/148‑1 (MQ=255)
                                                              aCGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATACTCAATAAATCCCCTttattatt                                                                                      >  1:2361036/1‑148 (MQ=255)
                                                                 cgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCGTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAAc                                                                                   >  1:2095041/1‑148 (MQ=255)
                                                                 cgcTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAAc                                                                                   >  1:1883370/1‑148 (MQ=255)
                                                                   cTGAACGAGGCCGCGGCGTGAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCAGATAGCCAATACGTGATTACCAGCAATACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGc                                                                                 <  1:3329854/148‑1 (MQ=255)
                                                                   cTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGc                                                                                 <  1:2874827/148‑1 (MQ=255)
                                                                    tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGcc                                                                                <  2:600876/148‑1 (MQ=255)
                                                                    tGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGAGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGcc                                                                                <  1:2648897/148‑1 (MQ=255)
                                                                     gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCa                                                                               <  2:1797229/148‑1 (MQ=255)
                                                                     gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCa                                                                               <  2:1492644/148‑1 (MQ=255)
                                                                     gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCa                                                                               <  2:1590089/148‑1 (MQ=255)
                                                                     gAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCa                                                                               <  1:2059171/148‑1 (MQ=255)
                                                                      aaCGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAt                                                                              <  2:356463/148‑1 (MQ=255)
                                                                       aCGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                             >  2:955551/1‑148 (MQ=255)
                                                                       aCGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                             >  1:1387860/1‑148 (MQ=255)
                                                                       aCGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCAtt                                                                             >  1:2088758/1‑148 (MQ=255)
                                                                       aCGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGGTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTGTATTATTAACGCCAtt                                                                             >  2:2723247/1‑148 (MQ=255)
                                                                        cGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTa                                                                            >  2:2037149/1‑148 (MQ=255)
                                                                          aGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAcc                                                                          <  2:1141091/148‑1 (MQ=255)
                                                                           ggCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTAccc                                                                         <  2:2911031/148‑1 (MQ=255)
                                                                           ggCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACATCAATTCCCCTTTTGGCCTCGCAGCCCTGGTTGCGGATACCCAATAAGTGATTAACAGCACTACTCCCGCTGAAATTCAATAAATCCCCTTTATTATAAACCCCATAAccc                                                                         >  1:1546741/1‑148 (MQ=255)
                                                                            gCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCt                                                                        >  1:1876768/1‑148 (MQ=255)
                                                                               gcgcCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGc                                                                     >  1:3385439/1‑148 (MQ=255)
                                                                                cgcCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGcc                                                                    >  1:1977998/1‑148 (MQ=255)
                                                                                   cGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaac                                                                 >  1:3461706/1‑148 (MQ=255)
                                                                                     tCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCcaacaa                                                               >  2:1138757/1‑148 (MQ=255)
                                                                                      cAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCaacaac                                                              >  1:2764531/1‑148 (MQ=255)
                                                                                       aGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAacaaca                                                             >  1:791277/1‑148 (MQ=255)
                                                                                         tGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATc                                                           >  1:1464398/1‑148 (MQ=255)
                                                                                         tGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATc                                                           >  1:1896584/1‑148 (MQ=255)
                                                                                         tGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCACCTTTATTATTAACGCCATTACCCTCGCCAACAACATc                                                           >  1:3308042/1‑148 (MQ=255)
                                                                                          ggTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCa                                                          >  1:1849372/1‑148 (MQ=255)
                                                                                            tttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGc                                                        >  1:1731801/1‑148 (MQ=255)
                                                                                             ttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCa                                                       <  2:2850461/148‑1 (MQ=255)
                                                                                             ttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCa                                                       >  2:3200973/1‑148 (MQ=255)
                                                                                             ttttACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCa                                                       <  2:1077523/148‑1 (MQ=255)
                                                                                              tttACCACTGTCAGAACTTCATTTCCCGTAGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCAc                                                      >  2:3315583/1‑148 (MQ=255)
                                                                                                tACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGc                                                    <  2:1069551/148‑1 (MQ=255)
                                                                                                tACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGc                                                    <  2:1957916/148‑1 (MQ=255)
                                                                                                  ccACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCat                                                  >  2:2129664/1‑148 (MQ=255)
                                                                                                       gTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCAtaataa                                             >  2:938684/1‑148 (MQ=255)
                                                                                                       gTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCAtaataa                                             <  2:3026461/148‑1 (MQ=255)
                                                                                                        tCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATaaa                                            >  2:3220197/1‑148 (MQ=255)
                                                                                                         cAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCTGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAg                                           <  2:1360638/148‑1 (MQ=255)
                                                                                                         cAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAg                                           >  2:3050038/1‑148 (MQ=255)
                                                                                                           gAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGt                                         <  1:3090546/148‑1 (MQ=255)
                                                                                                           gAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGt                                         <  1:3091099/148‑1 (MQ=255)
                                                                                                               ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTg                                     >  1:104211/1‑148 (MQ=255)
                                                                                                               ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTg                                     >  2:823284/1‑148 (MQ=255)
                                                                                                               ttCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTg                                     <  1:885894/148‑1 (MQ=255)
                                                                                                                  aTTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTa                                  <  2:478658/148‑1 (MQ=255)
                                                                                                                  aTTTCCCGTTGTGGCCTCGCCGCCCCCCCCCCCCATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTa                                  <  2:3388193/148‑1 (MQ=255)
                                                                                                                   tttCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTAc                                 <  2:1755629/148‑1 (MQ=255)
                                                                                                                     tCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCg                               <  2:3439396/148‑1 (MQ=255)
                                                                                                                     tCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCg                               <  2:2542850/148‑1 (MQ=255)
                                                                                                                         gTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCa                           >  1:1173154/1‑148 (MQ=255)
                                                                                                                         gTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCa                           <  2:225363/148‑1 (MQ=255)
                                                                                                                           tggcgCCACGCTGCCCACGTTGCCGAGAGCCAATACGTGATTACCACCACTACTCCCGCTGATATTCAATAAATCCCCATTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAgg                         <  2:2819317/144‑1 (MQ=255)
                                                                                                                               gCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTtct                     <  2:2088758/148‑1 (MQ=255)
                                                                                                                                   cGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTAc                 >  1:2809657/1‑148 (MQ=255)
                                                                                                                                   cGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTAc                 >  1:1435073/1‑148 (MQ=255)
                                                                                                                                        cccTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTa            <  1:13338/148‑1 (MQ=255)
                                                                                                                                            ggTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAAtgt        >  1:2242427/1‑148 (MQ=255)
                                                                                                                                                  ggATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCaaa  <  2:691789/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTCCCAGTTAGTGCCATTTTTACGCACATCGTACAGATATCCCCCCAACTCAACCTGAGAAGACGCGCTGAACGAGGCCGCGCCGTCAGTGGTTTTTACCACTGTCAGAACTTCATTTCCCGTTGTGGCCTCGCTGCCCTGGTTGCGGATAGCCAATACGTGATTACCAGCACTACTCCCGCTGATATTCAATAAATCCCCTTTATTATTAACGCCATTACCCTCGCCAACAACATCAGCACGCATAATAAAGGTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAA  >  CP009273/1249570‑1249863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑