Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,257,232 G→T 100% R34S (CGC→AGC)  prs ← phosphoribosylpyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,257,2320GT95.5% 623.6 / 17.7 212R34S (CGC→AGC) prsphosphoribosylpyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (6/4);  new base T (118/84);  total (124/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG  >  CP009273/1257087‑1257376
                                                                                                                                                 |                                                                                                                                                
gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTAc                                                                                                                                                <  2:2853319/148‑1 (MQ=255)
gCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTAc                                                                                                                                                <  2:2858837/148‑1 (MQ=255)
 cGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTAcc                                                                                                                                               <  2:3036775/148‑1 (MQ=255)
      gCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAg                                                                                                                                          >  2:3034036/1‑148 (MQ=255)
         cgacgCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAgcgg                                                                                                                                       <  2:14026/148‑1 (MQ=255)
                 ggCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCg                                                                                                                               >  1:3062318/1‑148 (MQ=255)
                 ggCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCg                                                                                                                               >  2:3257399/1‑148 (MQ=255)
                 ggCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATAGCTAAAGCTACCTACAGCGGCGTCGCCg                                                                                                                               >  2:2905941/1‑148 (MQ=255)
                      cAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCNCCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGa                                                                                                                          >  1:1806721/1‑148 (MQ=255)
                      cAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGa                                                                                                                          >  1:1740388/1‑148 (MQ=255)
                       aaCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGaa                                                                                                                         <  2:3193052/148‑1 (MQ=255)
                       aaCCATAACGACTAAGTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGaa                                                                                                                         <  2:3052624/148‑1 (MQ=255)
                        aCCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAg                                                                                                                        <  1:2384262/148‑1 (MQ=255)
                             aaCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTAc                                                                                                                   >  2:734640/1‑148 (MQ=255)
                              aCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACa                                                                                                                  >  2:3158189/1‑148 (MQ=255)
                              aCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACa                                                                                                                  >  1:3116462/1‑148 (MQ=255)
                              aCGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACa                                                                                                                  >  2:2331659/1‑148 (MQ=255)
                               cGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAg                                                                                                                 >  2:481084/1‑148 (MQ=255)
                               cGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAg                                                                                                                 >  1:544201/1‑148 (MQ=255)
                                gACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAgg                                                                                                                <  1:1939290/148‑1 (MQ=255)
                                 aCTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGc                                                                                                               <  2:816310/148‑1 (MQ=255)
                                  cTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCg                                                                                                              >  1:737353/1‑148 (MQ=255)
                                  cTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCCTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTCAAGTGTACAGGCg                                                                                                              >  1:3399722/1‑148 (MQ=255)
                                      ttCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTg                                                                                                          >  1:1048026/1‑148 (MQ=255)
                                      ttCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTg                                                                                                          >  2:3430050/1‑148 (MQ=255)
                                       tCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTgg                                                                                                         >  2:515531/1‑148 (MQ=255)
                                         cATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCa                                                                                                       >  1:2596560/1‑148 (MQ=255)
                                           tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAt                                                                                                     <  2:1040765/148‑1 (MQ=255)
                                           tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAt                                                                                                     >  2:2427052/1‑148 (MQ=255)
                                           tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAt                                                                                                     >  1:3140049/1‑148 (MQ=255)
                                           tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAt                                                                                                     <  1:1092715/148‑1 (MQ=255)
                                           tCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAAt                                                                                                     <  1:1262660/148‑1 (MQ=255)
                                             aGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATAc                                                                                                   >  1:1455805/1‑148 (MQ=255)
                                              ggTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACg                                                                                                  >  2:543614/1‑148 (MQ=255)
                                               gTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGt                                                                                                 <  1:1550683/148‑1 (MQ=255)
                                               gTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGt                                                                                                 <  2:1742241/148‑1 (MQ=255)
                                               gTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGt                                                                                                 >  1:766390/1‑148 (MQ=255)
                                                ttGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGtt                                                                                                <  1:2892068/148‑1 (MQ=255)
                                                ttGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGtt                                                                                                >  2:2213969/1‑148 (MQ=255)
                                                    cGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGc                                                                                            >  1:132371/1‑148 (MQ=255)
                                                    cGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGc                                                                                            >  2:1365695/1‑148 (MQ=255)
                                                       tagtagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAg                                                                                         >  1:118811/1‑148 (MQ=255)
                                                          tagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTc                                                                                      <  1:3365208/148‑1 (MQ=255)
                                                          tagGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTc                                                                                      <  2:1167089/148‑1 (MQ=255)
                                                            ggggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCg                                                                                    <  2:33547/148‑1 (MQ=255)
                                                              ggCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCggg                                                                                  <  2:3005036/148‑1 (MQ=255)
                                                                  cacgTGGACTGGATGCTGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGCAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGGTCCGGGGTgg                                                                              <  2:2052176/145‑1 (MQ=255)
                                                                    aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCg                                                                            >  2:2529005/1‑148 (MQ=255)
                                                                    aGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCg                                                                            <  2:3106425/148‑1 (MQ=255)
                                                                     gTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGt                                                                           >  2:1626491/1‑148 (MQ=255)
                                                                     gTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGt                                                                           <  1:1362226/148‑1 (MQ=255)
                                                                       ggACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTa                                                                         <  2:3475107/148‑1 (MQ=255)
                                                                         aCTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTAcc                                                                       <  2:2546182/148‑1 (MQ=255)
                                                                           tGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAg                                                                     >  1:473855/1‑148 (MQ=255)
                                                                            ggATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGc                                                                    >  1:2738854/1‑148 (MQ=255)
                                                                             gatgatGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCa                                                                   >  1:692907/1‑148 (MQ=255)
                                                                               tgatgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaa                                                                 >  1:2529893/1‑148 (MQ=255)
                                                                                 atgaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCaaaaa                                                               >  1:1591269/1‑148 (MQ=255)
                                                                                   gaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTGCCGGGGTGGCGTTACCAGCAAAAAGc                                                             >  1:2013786/1‑148 (MQ=255)
                                                                                   gaAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGc                                                             >  1:3057719/1‑148 (MQ=255)
                                                                                     aaaTATCACGTCCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTTGCAATACGTTGTGCTAGTTCCGGGGTGGAGTTACCAGCTAAAAGAtt                                                           <  2:2888816/148‑1 (MQ=255)
                                                                                      aaTATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTc                                                          >  2:1256122/1‑148 (MQ=255)
                                                                                       atatCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCa                                                         >  1:2288598/1‑148 (MQ=255)
                                                                                         atCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCata                                                       >  2:3120688/1‑148 (MQ=255)
                                                                                              caccGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAgg                                                  <  1:1119052/148‑1 (MQ=255)
                                                                                                  gcgTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACg                                              <  2:1417333/148‑1 (MQ=255)
                                                                                                     tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga                                           >  1:344194/1‑148 (MQ=255)
                                                                                                     tACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACgaga                                           >  2:1713920/1‑148 (MQ=255)
                                                                                                      aCATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                          <  2:65/148‑1 (MQ=255)
                                                                                                      aCATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaa                                          >  2:1539390/1‑148 (MQ=255)
                                                                                                       cATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGagaag                                         >  1:1930982/1‑148 (MQ=255)
                                                                                                           ttCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAAcc                                     >  1:905909/1‑148 (MQ=255)
                                                                                                            tCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCt                                    <  2:177246/148‑1 (MQ=255)
                                                                                                             cATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTc                                   >  2:757114/1‑148 (MQ=255)
                                                                                                             cATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTc                                   >  1:856584/1‑148 (MQ=255)
                                                                                                             cATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTc                                   >  1:1837297/1‑148 (MQ=255)
                                                                                                             cATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTc                                   >  1:857079/1‑148 (MQ=255)
                                                                                                              aTTAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCa                                  <  2:1352068/148‑1 (MQ=255)
                                                                                                               ttAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAg                                 >  2:2682119/1‑148 (MQ=255)
                                                                                                                tAATTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAgg                                >  1:1443776/1‑148 (MQ=255)
                                                                                                                 aaTTTGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGc                               >  1:1870457/1‑148 (MQ=255)
                                                                                                                    ttGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATg                            <  1:3142055/148‑1 (MQ=255)
                                                                                                                    ttGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATg                            >  2:1976718/1‑148 (MQ=255)
                                                                                                                    ttGTACGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATg                            >  1:2376739/1‑148 (MQ=255)
                                                                                                                        aCGCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTc                        <  2:3123338/148‑1 (MQ=255)
                                                                                                                          gCTGACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCa                      <  1:532676/148‑1 (MQ=255)
                                                                                                                             gACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                   <  1:3191941/148‑1 (MQ=255)
                                                                                                                             gACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                   >  1:982607/1‑148 (MQ=255)
                                                                                                                             gACTTCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtg                   <  2:2289/148‑1 (MQ=255)
                                                                                                                                ttCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtg                >  1:1631576/1‑148 (MQ=255)
                                                                                                                                ttCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtg                <  2:151709/148‑1 (MQ=255)
                                                                                                                                ttCGCCATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATtggtg                <  2:151571/148‑1 (MQ=255)
                                                                                                                                     cATCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAg           >  1:185030/1‑148 (MQ=255)
                                                                                                                                      aTCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGa          <  1:2427052/148‑1 (MQ=255)
                                                                                                                                      aTCGCTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGa          <  1:2858975/148‑1 (MQ=255)
                                                                                                                                          cTAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCt      <  2:2041767/148‑1 (MQ=255)
                                                                                                                                           tacaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTg     <  2:2887762/145‑1 (MQ=255)
                                                                                                                                           tAAAGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTg     >  1:2154666/1‑148 (MQ=255)
                                                                                                                                             aaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGcc   >  2:2640194/1‑148 (MQ=255)
                                                                                                                                             aaGCTACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGcc   <  2:2096119/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCGGAAGCACGACGCAGGGCATCAACCATAACGACTAATTCCATCAGGTTGTCGTTAGTAGGGGCACAAGTGGACTGGATGATGAAAATATCACCACCGCGTACATTTTCATTAATTTGTACGCTGACTTCGCCATCGCTAAAGCGACCTACAGCGGCGTCGCCGAGTGAAGTGTACAGGCGGTTGGCAATACGTTGTGCTAGTTCCGGGGTGGCGTTACCAGCAAAAAGCTTCATATCAGGCACGAGAAGAACCTCAGGCATGCGTCCATTGGTGGAAAGAATCTGCCG  >  CP009273/1257087‑1257376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑