Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,260,354 A→T 36.9% S395S (TCA→TCT)  hemA → glutamyl tRNA reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,260,3540AT36.9% 212.3 / 236.7 206S395S (TCA→TCT) hemAglutamyl tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (56/74);  new base T (32/44);  total (88/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

GTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCC  >  CP009273/1260210‑1260500
                                                                                                                                                |                                                                                                                                                  
gTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCtt                                                                                                                                                 >  2:3415490/1‑148 (MQ=255)
    cGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAAc                                                                                                                                             >  1:2816123/1‑148 (MQ=255)
    cGCAGCCAGGCAGAGCAACTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCATGCCATTATGCAGGATCTGTCATGGAAACTGACTAACCGCTTGATCCATGCGGCAACGAATTCACTTCAAc                                                                                                                                             <  2:2602541/148‑1 (MQ=255)
     gCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGTAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACa                                                                                                                                            <  2:1085563/148‑1 (MQ=255)
         ccAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGgcc                                                                                                                                        >  1:3277021/1‑148 (MQ=255)
            ggCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGgccgcc                                                                                                                                     >  2:2948744/1‑148 (MQ=255)
             gCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGccc                                                                                                                                    >  2:3349676/1‑148 (MQ=255)
              cAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCg                                                                                                                                   >  2:286538/1‑148 (MQ=255)
                 agCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAACTCTCTTCAACAGGCCGCCCGTGa                                                                                                                                >  2:2795200/1‑148 (MQ=255)
                 agCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGa                                                                                                                                <  2:1675863/148‑1 (MQ=255)
                     aGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACggg                                                                                                                            <  2:2490848/148‑1 (MQ=255)
                     aGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACggg                                                                                                                            >  1:1128922/1‑148 (MQ=255)
                      gTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTACCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACgggg                                                                                                                           <  1:1705991/148‑1 (MQ=255)
                         cgcgATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATa                                                                                                                        >  2:3142583/1‑148 (MQ=255)
                         cgcgATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATa                                                                                                                        <  1:2751440/148‑1 (MQ=255)
                         cgcgATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATa                                                                                                                        <  1:567367/148‑1 (MQ=255)
                         cgcgATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATa                                                                                                                        <  1:775001/148‑1 (MQ=255)
                         cgcgATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATa                                                                                                                        <  1:100511/148‑1 (MQ=255)
                           cgATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaac                                                                                                                      >  2:3315036/1‑148 (MQ=255)
                             aTGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATaacga                                                                                                                    >  1:1617548/1‑148 (MQ=255)
                              tGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaacgaa                                                                                                                   >  1:2522377/1‑148 (MQ=255)
                              tGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATATGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaacgaa                                                                                                                   <  1:2780040/148‑1 (MQ=255)
                              tGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATATGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaacgaa                                                                                                                   <  1:1105025/148‑1 (MQ=255)
                              tGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATATGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaacgaa                                                                                                                   <  1:2878131/148‑1 (MQ=255)
                               gAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaacgaac                                                                                                                  >  2:1935350/1‑148 (MQ=255)
                                aGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATaacgaacg                                                                                                                 >  2:11410/1‑148 (MQ=255)
                                 gTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGc                                                                                                                <  2:954563/148‑1 (MQ=255)
                                      ccGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACTGGGATAACGAACGCCTGaa                                                                                                           >  1:2216250/1‑148 (MQ=255)
                                      ccGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGaa                                                                                                           >  1:298035/1‑148 (MQ=255)
                                      cagCCAAAGCGTTAGCGGCCCTTGATCAGGGCGTCGTCGCGATCGCCATTATGCTTGATCTGGCATGTAAACTTAGTAACCGCTTGATCATTTCGACAACGAAATAACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGaa                                                                                                           <  2:209189/146‑1 (MQ=255)
                                       cGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAat                                                                                                          <  2:1812287/148‑1 (MQ=255)
                                       cGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAat                                                                                                          <  2:1154540/148‑1 (MQ=255)
                                        gCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAata                                                                                                         <  2:1830619/148‑1 (MQ=255)
                                            aaGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGACATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCt                                                                                                     >  2:107422/1‑148 (MQ=255)
                                            aaGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATCACGAACGCCTGAATATTCt                                                                                                     >  2:538844/1‑148 (MQ=255)
                                             aGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTg                                                                                                    >  2:2456945/1‑148 (MQ=255)
                                             aGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTg                                                                                                    >  1:687387/1‑148 (MQ=255)
                                                  tAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGa                                                                                               >  2:3075485/1‑148 (MQ=255)
                                                          cTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAAGCTCTGCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCg                                                                                       >  1:1131794/1‑148 (MQ=255)
                                                           ttGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCgg                                                                                      >  2:2398416/1‑148 (MQ=255)
                                                                cAGGGCGGCGTAGCGAAAGCCATTATTCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTgg                                                                                 <  1:3144073/148‑1 (MQ=255)
                                                                 aGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGa                                                                                <  1:1919776/148‑1 (MQ=255)
                                                                 aGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGa                                                                                <  1:2141775/148‑1 (MQ=255)
                                                                   ggcggcGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGt                                                                              <  1:2094615/148‑1 (MQ=255)
                                                                   ggcggcGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGt                                                                              >  1:1995379/1‑148 (MQ=255)
                                                                    gcggcgACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTa                                                                             <  2:512126/148‑1 (MQ=255)
                                                                       gcgACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCa                                                                          <  2:2199557/148‑1 (MQ=255)
                                                                        cgACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAg                                                                         <  2:1342064/148‑1 (MQ=255)
                                                                           cgcgCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAc                                                                      <  2:1414578/148‑1 (MQ=255)
                                                                            gcgcAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAca                                                                     >  2:3526053/1‑148 (MQ=255)
                                                                             cgcAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAcat                                                                    <  1:30547/148‑1 (MQ=255)
                                                                                aaGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAcatcat                                                                 >  2:1188438/1‑148 (MQ=255)
                                                                                 aGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAtt                                                                <  2:2970593/148‑1 (MQ=255)
                                                                                  gCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAttt                                                               <  1:910322/148‑1 (MQ=255)
                                                                                  gCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAttt                                                               >  1:963013/1‑148 (MQ=255)
                                                                                   ccATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAtttt                                                              <  1:486173/148‑1 (MQ=255)
                                                                                   ccATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACGTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAtttt                                                              <  1:485139/148‑1 (MQ=255)
                                                                                     aTTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCt                                                            >  2:2592706/1‑148 (MQ=255)
                                                                                      ttATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                                                           >  2:407449/1‑148 (MQ=255)
                                                                                      ttATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                                                           >  1:3188837/1‑148 (MQ=255)
                                                                                      ttATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                                                           <  2:769126/148‑1 (MQ=255)
                                                                                      ttATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                                                           <  1:3072682/148‑1 (MQ=255)
                                                                                      ttATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                                                           <  1:2737883/148‑1 (MQ=255)
                                                                                        aTGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtttt                                                         <  2:128907/148‑1 (MQ=255)
                                                                                        aTGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtttt                                                         <  2:268159/148‑1 (MQ=255)
                                                                                        aTGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtttt                                                         <  2:3010364/148‑1 (MQ=255)
                                                                                         tGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttt                                                        >  2:1102278/1‑148 (MQ=255)
                                                                                          gCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtttttt                                                       <  2:2028901/148‑1 (MQ=255)
                                                                                              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa                                                   >  2:2307405/1‑148 (MQ=255)
                                                                                              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa                                                   >  1:232201/1‑148 (MQ=255)
                                                                                              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa                                                   >  1:229892/1‑148 (MQ=255)
                                                                                                 cTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAggg                                                <  1:3016779/148‑1 (MQ=255)
                                                                                                  tGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt                                               <  2:1128922/148‑1 (MQ=255)
                                                                                                    gCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGc                                             <  1:1559665/148‑1 (MQ=255)
                                                                                                     cATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCa                                            <  1:3161247/148‑1 (MQ=255)
                                                                                                     cATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCa                                            >  2:3467180/1‑148 (MQ=255)
                                                                                                       tGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCAtt                                          <  1:3518701/148‑1 (MQ=255)
                                                                                                       tGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCAGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCAtt                                          >  2:1634166/1‑148 (MQ=255)
                                                                                                        ggAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCAttt                                         >  1:1447633/1‑148 (MQ=255)
                                                                                                           aaCTGACTAACCGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACg                                      <  1:1333104/148‑1 (MQ=255)
                                                                                                             cTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGcc                                    >  2:582088/1‑148 (MQ=255)
                                                                                                                      cGCTTGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGcc                           <  2:2235673/148‑1 (MQ=255)
                                                                                                                      cGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGcc                           <  2:3258464/148‑1 (MQ=255)
                                                                                                                          tGATCCATGCGCCAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCt                       <  2:1223754/148‑1 (MQ=255)
                                                                                                                          tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCt                       <  1:2597613/148‑1 (MQ=255)
                                                                                                                            aTCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTAt                     >  2:1043914/1‑148 (MQ=255)
                                                                                                                              ccATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCg                   >  2:3418378/1‑148 (MQ=255)
                                                                                                                               cATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGt                  <  2:1458896/148‑1 (MQ=255)
                                                                                                                               cATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGt                  <  2:585313/148‑1 (MQ=255)
                                                                                                                               cATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGt                  <  2:1880218/148‑1 (MQ=255)
                                                                                                                                 tGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTg                >  1:1154525/1‑148 (MQ=255)
                                                                                                                                 tGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTg                >  1:2966047/1‑148 (MQ=255)
                                                                                                                                      cAACGAACTCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCaaa           <  2:875821/148‑1 (MQ=255)
                                                                                                                                      cAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCaaa           <  2:2859778/148‑1 (MQ=255)
                                                                                                                                      cAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCaaa           <  1:2506994/148‑1 (MQ=255)
                                                                                                                                      cAACGAAATCTCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCaaa           >  2:3101373/1‑148 (MQ=255)
                                                                                                                                              tCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACCTCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGc   >  1:354570/1‑148 (MQ=255)
                                                                                                                                              tCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGc   >  1:354788/1‑148 (MQ=255)
                                                                                                                                               cACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGcc  <  1:2714214/148‑1 (MQ=255)
                                                                                                                                               cACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGcc  >  1:963044/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GTATCGCAGCCAGGCAGAGCAAGTTCGCGATGAGTTAACCGCCAAAGCGTTAGCGGCCCTTGAGCAGGGCGGCGACGCGCAAGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCC  >  CP009273/1260210‑1260500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑