Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,322,140 T→A 100% T159S (ACG→TCG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,322,1400TA98.9% 595.0 / ‑2.4 179T159S (ACG→TCG) btuRcob(I)yrinic acid a,c‑diamide adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (99/78);  total (100/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 181 total aligned reads displayed.

CCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATG  >  CP009273/1321995‑1322284
                                                                                                                                                 |                                                                                                                                                
ccGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCgaga                                                                                                                                                >  2:3064426/1‑148 (MQ=255)
ccGGACGTCATATCCGGCATTTTTACAGATTAATAATAGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGAAAACCACGACCCgaga                                                                                                                                                >  1:991102/1‑148 (MQ=255)
 cGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGAt                                                                                                                                               <  1:1822264/148‑1 (MQ=255)
           atCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTc                                                                                                                                     <  1:1219566/148‑1 (MQ=255)
             ccGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTg                                                                                                                                   <  2:3375949/148‑1 (MQ=255)
             ccGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTg                                                                                                                                   >  2:1605453/1‑148 (MQ=255)
                gCATTTTTACAGATTAATACTCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTg                                                                                                                                <  2:2706876/148‑1 (MQ=255)
                gCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTg                                                                                                                                <  2:2685905/148‑1 (MQ=255)
                  aTTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAt                                                                                                                              <  1:1311360/148‑1 (MQ=255)
                   tttttACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtg                                                                                                                             <  1:717674/148‑1 (MQ=255)
                   tttttACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtg                                                                                                                             <  2:2509376/148‑1 (MQ=255)
                    ttttACATATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGAtgt                                                                                                                            <  2:742690/148‑1 (MQ=255)
                      ttACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTgg                                                                                                                          >  2:1700732/1‑148 (MQ=255)
                       tACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGa                                                                                                                         >  1:2704022/1‑148 (MQ=255)
                         cAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACg                                                                                                                       <  1:2340844/148‑1 (MQ=255)
                           gATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGtt                                                                                                                     <  1:1748484/148‑1 (MQ=255)
                           gATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGtt                                                                                                                     >  1:2864776/1‑148 (MQ=255)
                             ttAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCa                                                                                                                   >  2:2989499/1‑148 (MQ=255)
                              taataaTCGATACCTATCTGCGCTTTTACACCGGCATCAAACGAATGTTTGACGGGGCGTAATTGACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCTCGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCAt                                                                                                                  <  2:2009987/148‑1 (MQ=255)
                                 taatCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTa                                                                                                               >  1:253816/1‑148 (MQ=255)
                                  actCGATCCCTAGCTGCGCTTTTACACCGGCATCAACCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTaa                                                                                                              <  1:779246/146‑1 (MQ=255)
                                    tCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACg                                                                                                            <  2:1517738/148‑1 (MQ=255)
                                     cGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCNAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGc                                                                                                           >  1:1806050/1‑148 (MQ=255)
                                       aTCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCt                                                                                                         >  2:1405982/1‑148 (MQ=255)
                                             aTCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcacca                                                                                                   <  2:1919243/148‑1 (MQ=255)
                                             aTCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcacca                                                                                                   >  2:2106290/1‑148 (MQ=255)
                                              tCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGcaccac                                                                                                  <  2:2376538/148‑1 (MQ=255)
                                               cTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCAct                                                                                                 >  1:3221211/1‑148 (MQ=255)
                                               cTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCAct                                                                                                 >  1:2374031/1‑148 (MQ=255)
                                                    cTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACttcttc                                                                                            >  1:1010280/1‑148 (MQ=255)
                                                     ttttACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                           >  1:862228/1‑148 (MQ=255)
                                                     ttttACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTAAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTcc                                                                                           <  1:3059474/148‑1 (MQ=255)
                                                        tACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGt                                                                                        <  1:1781953/148‑1 (MQ=255)
                                                           acCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGc                                                                                     >  1:2150652/1‑148 (MQ=255)
                                                               gCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAAt                                                                                 <  2:2512708/148‑1 (MQ=255)
                                                               gCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAAt                                                                                 <  1:2820912/148‑1 (MQ=255)
                                                                cATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATa                                                                                >  2:2049546/1‑148 (MQ=255)
                                                                  tCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                              <  2:126523/148‑1 (MQ=255)
                                                                  tCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                              <  1:1187484/148‑1 (MQ=255)
                                                                  tCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGt                                                                              >  1:1001421/1‑148 (MQ=255)
                                                                  tCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCCTCCAGTGGCAAATAGt                                                                              <  1:1543587/148‑1 (MQ=255)
                                                                   cAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTc                                                                             <  1:3299123/148‑1 (MQ=255)
                                                                   cAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTc                                                                             <  1:539833/148‑1 (MQ=255)
                                                                    aaaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCa                                                                            >  1:1862910/1‑148 (MQ=255)
                                                                     aaCGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCAt                                                                           >  1:1685162/1‑148 (MQ=255)
                                                                        gCATGTTTGTCGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACg                                                                        <  2:1198432/148‑1 (MQ=255)
                                                                         cATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGc                                                                       >  2:2331580/1‑148 (MQ=255)
                                                                                 aCGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCatat                                                               <  1:2489948/148‑1 (MQ=255)
                                                                                  cGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCAtata                                                              <  1:1758616/148‑1 (MQ=255)
                                                                                      gCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTc                                                          <  2:3539699/148‑1 (MQ=255)
                                                                                      gCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTc                                                          <  2:1130428/148‑1 (MQ=255)
                                                                                       cGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGAGGCAAATAGTCATACGCCACCATATACGTCa                                                         <  1:1226056/148‑1 (MQ=255)
                                                                                         tAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGt                                                       <  2:1950435/148‑1 (MQ=255)
                                                                                          aaTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGtt                                                      <  1:3124935/148‑1 (MQ=255)
                                                                                          aaTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGtt                                                      >  2:1827772/1‑148 (MQ=255)
                                                                                          aaTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGtt                                                      >  2:62108/1‑148 (MQ=255)
                                                                                           aTTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtc                                                     <  1:183867/148‑1 (MQ=255)
                                                                                            ttCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtca                                                    <  2:1812219/148‑1 (MQ=255)
                                                                                             tCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcat                                                   >  1:418242/1‑148 (MQ=255)
                                                                                               aCTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTtcatca                                                 <  2:418242/148‑1 (MQ=255)
                                                                                                 ttACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAg                                               >  2:2871139/1‑148 (MQ=255)
                                                                                                        aTCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAcc                                        >  1:2677015/1‑148 (MQ=255)
                                                                                                        aTCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAAcc                                        <  2:492695/148‑1 (MQ=255)
                                                                                                         tCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCa                                       >  1:1563317/1‑148 (MQ=255)
                                                                                                            gCCAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCatat                                    >  1:2019458/1‑148 (MQ=255)
                                                                                                             ccAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATc                                   <  2:1959566/148‑1 (MQ=255)
                                                                                                              cAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc                                  >  2:1503746/1‑148 (MQ=255)
                                                                                                              cAGTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATcc                                  <  1:1444872/148‑1 (MQ=255)
                                                                                                                tttCAATAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAg                                <  1:2331580/147‑1 (MQ=255)
                                                                                                                gTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAg                                <  1:2240828/148‑1 (MQ=255)
                                                                                                                gTTCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCAACACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAg                                >  2:2902269/1‑148 (MQ=255)
                                                                                                                 ttCAAGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGt                               >  1:352498/1‑148 (MQ=255)
                                                                                                                    aaGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAg                            <  2:1405920/148‑1 (MQ=255)
                                                                                                                     aGAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAgg                           >  2:901601/1‑148 (MQ=255)
                                                                                                                      gAATATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGa                          <  1:70160/148‑1 (MQ=255)
                                                                                                                       aaTATCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGaa                         >  2:151384/1‑148 (MQ=255)
                                                                                                                        atatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAAt                        <  1:1580972/148‑1 (MQ=255)
                                                                                                                         tatCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATc                       >  2:1905940/1‑148 (MQ=255)
                                                                                                                           tCCCGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCag                     >  2:3258926/1‑148 (MQ=255)
                                                                                                                            cccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCACAACCATATCCAGTGAGGAATCagc                    >  2:3093407/1‑148 (MQ=255)
                                                                                                                            cccGATGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagc                    >  2:3032900/1‑148 (MQ=255)
                                                                                                                                 tGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGACTCagcaagca               >  1:1959406/1‑148 (MQ=255)
                                                                                                                                 tGACAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCagcaagca               >  1:1076557/1‑148 (MQ=255)
                                                                                                                                    cAACCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATcc            >  1:2899617/1‑148 (MQ=255)
                                                                                                                                     aaCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCg           <  2:1685633/148‑1 (MQ=255)
                                                                                                                                     aaCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCg           >  2:2356578/1‑148 (MQ=255)
                                                                                                                                     aaCCACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCg           <  2:1685162/148‑1 (MQ=255)
                                                                                                                                       ccACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt         <  1:945093/148‑1 (MQ=255)
                                                                                                                                       ccACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt         >  1:1967648/1‑148 (MQ=255)
                                                                                                                                       ccACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt         >  1:532350/1‑148 (MQ=255)
                                                                                                                                       ccACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt         >  2:1265882/1‑148 (MQ=255)
                                                                                                                                       ccACGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCt         >  2:1599007/1‑148 (MQ=255)
                                                                                                                                         aCGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCttt       >  2:1058377/1‑148 (MQ=255)
                                                                                                                                          cGACCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTg      >  2:567452/1‑148 (MQ=255)
                                                                                                                                            aCCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCa    >  1:872958/1‑148 (MQ=255)
                                                                                                                                            aCCCGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCa    >  1:1099086/1‑148 (MQ=255)
                                                                                                                                             cccGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCAt   >  2:2414545/1‑148 (MQ=255)
                                                                                                                                              ccGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATg  <  1:2106290/148‑1 (MQ=255)
                                                                                                                                              ccGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATg  >  2:2150284/1‑148 (MQ=255)
                                                                                                                                              ccGAGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCAACCGCTTTGCATg  >  2:3106643/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGGACGTCATATCCGGCATTTTTACAGATTAATAATCGATCCCTATCTGCGCTTTTACACCGGCATCAAACGCATGTTTGACGGGGCGTAATTCACTTACCGTATCTGCCAGTTCAAGAATATCCCGATGACAACCACGACCCGTGATAATCACCGTCTGTTGATGTGGACGTTCATTTAACGCCTGCACCACTTCTTCCAGTGGCAAATAGTCATACGCCACCATATACGTCAGTTCATCAAGCAAAACCATATCCAGTGAGGAATCAGCAAGCATCCGCTTTGCATG  >  CP009273/1321995‑1322284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑