Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,341,886 T→A 13.8% A428A (GCA→GCT)  rnb ← ribonuclease II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,341,8860TA13.8% 620.0 / 75.7 216A428A (GCA→GCT) rnbribonuclease II
Reads supporting (aligned to +/- strand):  ref base T (82/104);  new base A (14/16);  total (96/120)
Fisher's exact test for biased strand distribution p-value = 8.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

ATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTC  >  CP009273/1341740‑1342032
                                                                                                                                                  |                                                                                                                                                  
aTTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTg                                                                                                                                                   >  1:2187752/1‑148 (MQ=255)
 tttCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGc                                                                                                                                                  >  2:79174/1‑148 (MQ=255)
  ttCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGcc                                                                                                                                                 <  2:282693/148‑1 (MQ=255)
  ttCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGcc                                                                                                                                                 >  1:2941685/1‑148 (MQ=255)
      gCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAgcg                                                                                                                                             >  1:2872014/1‑148 (MQ=255)
        aaaTGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGcgcg                                                                                                                                           <  2:1330665/148‑1 (MQ=255)
         aaTGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGt                                                                                                                                          <  1:134420/148‑1 (MQ=255)
          aTGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTc                                                                                                                                         >  1:243750/1‑148 (MQ=255)
          aTGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTc                                                                                                                                         >  1:2847292/1‑148 (MQ=255)
           tGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCg                                                                                                                                        >  2:1808969/1‑148 (MQ=255)
            gACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCgg                                                                                                                                       <  2:620/148‑1 (MQ=255)
             aCTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGc                                                                                                                                      <  1:3484241/148‑1 (MQ=255)
             aCTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGc                                                                                                                                      >  2:2646764/1‑148 (MQ=255)
             aCTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGc                                                                                                                                      <  1:814499/148‑1 (MQ=255)
              cTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCa                                                                                                                                     <  2:3187306/148‑1 (MQ=255)
               tGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCAt                                                                                                                                    <  2:3032013/148‑1 (MQ=255)
                 gAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTc                                                                                                                                  >  2:2834242/1‑148 (MQ=255)
                  aaGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCg                                                                                                                                 >  2:2088988/1‑148 (MQ=255)
                    gCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGcc                                                                                                                               >  2:77841/1‑148 (MQ=255)
                     cgacgaATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCg                                                                                                                              >  2:3364393/1‑148 (MQ=255)
                      gacgaATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCgg                                                                                                                             >  2:37586/1‑148 (MQ=255)
                       acgaATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGa                                                                                                                            >  2:2743349/1‑148 (MQ=255)
                       acgaATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGa                                                                                                                            <  1:682233/148‑1 (MQ=255)
                       acgaATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGa                                                                                                                            <  1:2905862/148‑1 (MQ=255)
                           aTGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCaa                                                                                                                        <  1:1652817/148‑1 (MQ=255)
                             gCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAg                                                                                                                      <  1:2295726/148‑1 (MQ=255)
                               ggCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGcc                                                                                                                    <  1:547433/148‑1 (MQ=255)
                                 cTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGGTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCa                                                                                                                  <  1:321011/148‑1 (MQ=255)
                                  tGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCat                                                                                                                 >  2:1067120/1‑148 (MQ=255)
                                  tGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCat                                                                                                                 <  1:104797/148‑1 (MQ=255)
                                  tGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCat                                                                                                                 <  2:488074/148‑1 (MQ=255)
                                   gTCGAGGAAACCAGGTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTGTCAGCAACGCTGCCAGCGCGTCGGCATGCGCCGGATCAAAGCCCaga                                                                                                                >  2:1658600/1‑146 (MQ=255)
                                    tCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCatat                                                                                                               >  1:1558803/1‑148 (MQ=255)
                                      gAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGc                                                                                                             <  2:1219951/148‑1 (MQ=255)
                                       aGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCa                                                                                                            <  2:1196922/148‑1 (MQ=255)
                                       aGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCa                                                                                                            <  1:2583497/148‑1 (MQ=255)
                                         gAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACg                                                                                                          <  2:499557/148‑1 (MQ=255)
                                          aaaCCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGt                                                                                                         <  2:1449376/148‑1 (MQ=255)
                                            aCCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTta                                                                                                       <  1:1773708/148‑1 (MQ=255)
                                            aCCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTta                                                                                                       <  1:940991/148‑1 (MQ=255)
                                                gttggttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGa                                                                                                   <  2:3356140/148‑1 (MQ=255)
                                                  tggttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATg                                                                                                 >  1:3235413/1‑148 (MQ=255)
                                                  tggttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATg                                                                                                 <  2:963732/148‑1 (MQ=255)
                                                   ggttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGc                                                                                                >  2:3119269/1‑148 (MQ=255)
                                                   ggttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGc                                                                                                >  2:2312428/1‑148 (MQ=255)
                                                     ttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCa                                                                                              >  1:3305172/1‑148 (MQ=255)
                                                     ttgCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCa                                                                                              >  1:3453585/1‑148 (MQ=255)
                                                       gcgcGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCaaa                                                                                            <  2:137840/148‑1 (MQ=255)
                                                        cgcgTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCaaaa                                                                                           <  2:2611110/148‑1 (MQ=255)
                                                        cgcgTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCaaaa                                                                                           <  1:2743596/148‑1 (MQ=255)
                                                        cgcgTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCaaaa                                                                                           <  1:623232/148‑1 (MQ=255)
                                                          cgTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAAcc                                                                                         >  1:355973/1‑148 (MQ=255)
                                                             ccAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAg                                                                                      >  1:2961713/1‑148 (MQ=255)
                                                                  tCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTAt                                                                                 >  2:3157582/1‑148 (MQ=255)
                                                                  tCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTAt                                                                                 >  2:3372171/1‑148 (MQ=255)
                                                                  tCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGGTATAGATGCCAAAACCGAGCTTAt                                                                                 >  1:3482678/1‑148 (MQ=255)
                                                                  tCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTAt                                                                                 >  1:1786565/1‑148 (MQ=255)
                                                                       acgCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCgcgc                                                                            <  1:1881842/148‑1 (MQ=255)
                                                                         gCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAg                                                                          >  2:2805830/1‑148 (MQ=255)
                                                                           aGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTa                                                                        <  2:1732726/148‑1 (MQ=255)
                                                                           aGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTa                                                                        <  2:2170350/148‑1 (MQ=255)
                                                                             tttGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTAcg                                                                      >  1:1158230/1‑148 (MQ=255)
                                                                                 cAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCggg                                                                  <  1:2083327/148‑1 (MQ=255)
                                                                                      aCCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGca                                                             <  2:226716/148‑1 (MQ=255)
                                                                                      aCCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGca                                                             >  2:1234574/1‑148 (MQ=255)
                                                                                      aCCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGca                                                             >  1:2052809/1‑148 (MQ=255)
                                                                                        cGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGcaca                                                           >  1:3308328/1‑148 (MQ=255)
                                                                                          tCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACaaa                                                         <  2:2194906/148‑1 (MQ=255)
                                                                                          tCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACaaa                                                         <  2:1787347/148‑1 (MQ=255)
                                                                                           ccAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAAt                                                        <  1:3339037/148‑1 (MQ=255)
                                                                                               cGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTa                                                    <  2:2296309/148‑1 (MQ=255)
                                                                                               cGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTa                                                    >  2:2999946/1‑148 (MQ=255)
                                                                                                 tGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGc                                                  <  1:2262533/148‑1 (MQ=255)
                                                                                                  gAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCg                                                 <  1:1937995/148‑1 (MQ=255)
                                                                                                   aGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCgg                                                <  1:79174/148‑1 (MQ=255)
                                                                                                     cACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCa                                              >  1:114642/1‑148 (MQ=255)
                                                                                                         tcttcGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAatca                                          <  1:1893746/148‑1 (MQ=255)
                                                                                                         tcttcGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAatca                                          <  2:2941685/148‑1 (MQ=255)
                                                                                                         tcttcGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAatca                                          <  1:94831/148‑1 (MQ=255)
                                                                                                            tcGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCg                                       <  2:1576927/148‑1 (MQ=255)
                                                                                                               gCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGctt                                    <  2:2478126/148‑1 (MQ=255)
                                                                                                                cATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGcttc                                   <  2:1838096/148‑1 (MQ=255)
                                                                                                                cATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGcttc                                   <  2:2996915/148‑1 (MQ=255)
                                                                                                                   cGACATGCAGACCGTGCGTGGTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCttcttc                                <  1:3144565/148‑1 (MQ=255)
                                                                                                                    gACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTcg                               <  2:2207073/148‑1 (MQ=255)
                                                                                                                     aCATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTcga                              <  1:1716385/148‑1 (MQ=255)
                                                                                                                     aCATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTcga                              <  1:762803/148‑1 (MQ=255)
                                                                                                                         gCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGAt                          <  1:1524679/148‑1 (MQ=255)
                                                                                                                            gACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACg                       >  2:833913/1‑148 (MQ=255)
                                                                                                                                gTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTg                   <  2:3309887/148‑1 (MQ=255)
                                                                                                                                 tGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTgg                  >  2:2933081/1‑148 (MQ=255)
                                                                                                                                   cGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCa                <  1:2805830/148‑1 (MQ=255)
                                                                                                                                     ttttCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAAt              <  2:1338806/148‑1 (MQ=255)
                                                                                                                                      tttCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATg             >  2:3488025/1‑148 (MQ=255)
                                                                                                                                           gCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGcgacg        <  1:1271393/148‑1 (MQ=255)
                                                                                                                                           gCAACGCTGCCAGCGCGTCGACATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGcgacg        <  1:1001832/148‑1 (MQ=255)
                                                                                                                                            cAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGcgacga       >  2:710865/1‑148 (MQ=255)
                                                                                                                                              aCGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAgg     >  2:212466/1‑148 (MQ=255)
                                                                                                                                              aCGCAGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAgg     <  1:1442724/148‑1 (MQ=255)
                                                                                                                                                gCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCt   <  1:1094369/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATTTCAGCAAATGACTGGAAGCGACGAATGCGGCTGTCGAGGAAACCAGTTGGTTGCGCGTCCAGTTCACGACGCAGTTTGCAGAAACCGTCCAGCGTGAGCACTTCTTCGGCATCGACATGCAGACCGTGCGTTTTCAGCAACGCTGCCAGCGCGTCGGCATTCGCCGGATCAAAGCCCATATGCACGTTATAGATGCCAAAACCGAGCTTATCGCGCAGTACGCGGGCCGCACAAATGTTAGCGGCAATCATCGCTTCTTCGACGATACGGTTGGCAATGCGACGAGGCTC  >  CP009273/1341740‑1342032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑