Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,350,891 G→T 100% S159R (AGC→AGA)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,350,8910GT99.4% 564.1 / ‑4.1 171S159R (AGC→AGA) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (87/82);  total (88/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

GTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAA  >  CP009273/1350746‑1351034
                                                                                                                                                 |                                                                                                                                               
gTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGActct                                                                                                                                               <  1:1061007/148‑1 (MQ=255)
 ttGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCtt                                                                                                                                              >  2:1806301/1‑148 (MQ=255)
 ttGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCtt                                                                                                                                              >  2:393944/1‑148 (MQ=255)
   gCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCtttt                                                                                                                                            >  2:174936/1‑148 (MQ=255)
   gCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCtttt                                                                                                                                            <  2:44301/148‑1 (MQ=255)
   gCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCtttt                                                                                                                                            >  1:698648/1‑148 (MQ=255)
   gCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCtttt                                                                                                                                            >  2:3200578/1‑148 (MQ=255)
     tCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCa                                                                                                                                          <  1:1134833/148‑1 (MQ=255)
     tCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTGTGCGGACTCTTTTCa                                                                                                                                          >  2:2560057/1‑148 (MQ=255)
          gCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTa                                                                                                                                     >  2:1439582/1‑148 (MQ=255)
          gCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTa                                                                                                                                     <  2:1618436/148‑1 (MQ=255)
          gCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTa                                                                                                                                     >  1:112144/1‑148 (MQ=255)
             aTCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCg                                                                                                                                  >  2:3060647/1‑148 (MQ=255)
                ttctttctCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCa                                                                                                                               >  2:3090767/1‑148 (MQ=255)
                 tctttctCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCaa                                                                                                                              >  2:1726120/1‑148 (MQ=255)
                 tctttctCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCaa                                                                                                                              >  2:2140315/1‑148 (MQ=255)
                     tctcTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTg                                                                                                                          <  2:2252456/148‑1 (MQ=255)
                       tctAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCa                                                                                                                        >  2:691061/1‑148 (MQ=255)
                        ctAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAg                                                                                                                       <  1:360756/148‑1 (MQ=255)
                          aaCTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGc                                                                                                                     <  2:1585421/148‑1 (MQ=255)
                          aaCTTCCGGGCATATTCTGCCGACATGACCGAAGCATAAGGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGc                                                                                                                     >  1:1009971/1‑148 (MQ=255)
                          aaCGTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGc                                                                                                                     <  1:757104/148‑1 (MQ=255)
                           aCTTCCGGTCATATTCTGCCGACATGACCGAAGCTTAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCt                                                                                                                    <  1:1278387/148‑1 (MQ=255)
                           aCTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCt                                                                                                                    >  1:1471947/1‑148 (MQ=255)
                           aCTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCt                                                                                                                    >  2:100982/1‑148 (MQ=255)
                             ttCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGt                                                                                                                  <  1:1920139/148‑1 (MQ=255)
                              tCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTc                                                                                                                 >  1:1434369/1‑148 (MQ=255)
                                 gggCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGaa                                                                                                              <  1:1797827/148‑1 (MQ=255)
                                 gggCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGaa                                                                                                              <  1:1797791/148‑1 (MQ=255)
                                 gggCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGaa                                                                                                              >  2:3472834/1‑148 (MQ=255)
                                   gCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGt                                                                                                            <  1:2061926/148‑1 (MQ=255)
                                   gCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGt                                                                                                            >  1:3344045/1‑148 (MQ=255)
                                      tatTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAgg                                                                                                         >  1:1674930/1‑148 (MQ=255)
                                       atTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAggg                                                                                                        >  2:2246020/1‑148 (MQ=255)
                                        ttCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAgggg                                                                                                       <  1:912369/148‑1 (MQ=255)
                                        ttCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAgggg                                                                                                       >  2:1204160/1‑148 (MQ=255)
                                           tGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAg                                                                                                    <  1:1166633/148‑1 (MQ=255)
                                              cGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTtg                                                                                                 <  2:981440/148‑1 (MQ=255)
                                               gACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTtgc                                                                                                <  1:2304904/148‑1 (MQ=255)
                                                  aTGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTtgctgc                                                                                             <  2:3477829/148‑1 (MQ=255)
                                                  aTGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTtgctgc                                                                                             >  2:3395767/1‑148 (MQ=255)
                                                    gACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCg                                                                                           <  1:402699/148‑1 (MQ=255)
                                                         aaGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGGTGCTGCCGTTGAc                                                                                      >  1:1815015/1‑148 (MQ=255)
                                                            cATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGtt                                                                                   >  1:257408/1‑148 (MQ=255)
                                                            cATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGtt                                                                                   <  2:1842670/148‑1 (MQ=255)
                                                              tAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGACGTAGGGGAAGTTGCTGCCGTTGACGTTAt                                                                                 >  1:2736447/1‑148 (MQ=255)
                                                               aaTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATg                                                                                <  2:1813629/148‑1 (MQ=255)
                                                               aaTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATg                                                                                >  2:495377/1‑148 (MQ=255)
                                                               aaTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATg                                                                                <  2:2631118/148‑1 (MQ=255)
                                                               aaTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATg                                                                                <  2:1878308/148‑1 (MQ=255)
                                                                   ggTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCAc                                                                            >  1:3270807/1‑148 (MQ=255)
                                                                   ggTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCAc                                                                            <  1:469608/148‑1 (MQ=255)
                                                                       gCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGgt                                                                        <  1:2532038/148‑1 (MQ=255)
                                                                        cGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGgtt                                                                       <  2:2887842/148‑1 (MQ=255)
                                                                         gAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGgttg                                                                      <  1:419479/148‑1 (MQ=255)
                                                                            gTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGttgttg                                                                   >  2:941574/1‑148 (MQ=255)
                                                                             tGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGc                                                                  >  1:2006632/1‑148 (MQ=255)
                                                                             tGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGc                                                                  >  1:2499722/1‑148 (MQ=255)
                                                                               ccACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCgg                                                                >  1:1892042/1‑148 (MQ=255)
                                                                                cacaAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGt                                                               >  2:315529/1‑148 (MQ=255)
                                                                                cacaAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGt                                                               >  2:1026569/1‑148 (MQ=255)
                                                                                  caAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCa                                                             >  2:2033931/1‑148 (MQ=255)
                                                                                   aaaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaa                                                            <  2:2137774/148‑1 (MQ=255)
                                                                                    aaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaaa                                                           >  2:1735105/1‑148 (MQ=255)
                                                                                     aaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaaaa                                                          <  1:1261719/148‑1 (MQ=255)
                                                                                     aaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaaaa                                                          <  1:1332028/148‑1 (MQ=255)
                                                                                      aaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaaaaa                                                         >  1:521155/1‑148 (MQ=255)
                                                                                       aagaagCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAAt                                                        >  2:2235518/1‑148 (MQ=255)
                                                                                        agaagCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAAtt                                                       >  2:2829066/1‑148 (MQ=255)
                                                                                          aagCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCg                                                     >  1:1842681/1‑148 (MQ=255)
                                                                                          aagCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCg                                                     <  1:3145741/148‑1 (MQ=255)
                                                                                             cATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTg                                                  >  2:2760127/1‑148 (MQ=255)
                                                                                               tCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGa                                                >  2:2974622/1‑148 (MQ=255)
                                                                                                ccGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGaa                                               >  2:1247907/1‑148 (MQ=255)
                                                                                                   gCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGt                                            >  1:1150331/1‑148 (MQ=255)
                                                                                                    cTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTa                                           <  1:100982/148‑1 (MQ=255)
                                                                                                     tGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTGTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaa                                          <  2:1720005/148‑1 (MQ=255)
                                                                                                      gggCGATTCGGAACTCAACGGTATGATTATCCAGGTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGTGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaaa                                         <  2:577860/148‑1 (MQ=255)
                                                                                                      gggCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaaa                                         >  2:97165/1‑148 (MQ=255)
                                                                                                      gggCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaaa                                         >  2:907535/1‑148 (MQ=255)
                                                                                                      gggCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaaa                                         >  2:2614874/1‑148 (MQ=255)
                                                                                                      gggCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaaa                                         >  2:2485603/1‑148 (MQ=255)
                                                                                                       ggCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAac                                        >  1:2808861/1‑148 (MQ=255)
                                                                                                             tCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACcaca                                  <  1:3443281/148‑1 (MQ=255)
                                                                                                             tCGGAACTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACcaca                                  <  1:295248/148‑1 (MQ=255)
                                                                                                                  aCTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtcgtc                             <  1:174936/148‑1 (MQ=255)
                                                                                                                  aCTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtcgtc                             >  1:1537887/1‑148 (MQ=255)
                                                                                                                  aCTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtcgtc                             <  2:1146008/148‑1 (MQ=255)
                                                                                                                   cTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtcgtcg                            <  2:3149590/148‑1 (MQ=255)
                                                                                                                   cTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtcgtcg                            <  2:312816/148‑1 (MQ=255)
                                                                                                                   cTCAACGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtcgtcg                            >  2:834824/1‑148 (MQ=255)
                                                                                                                      aaCGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGca                         <  2:268835/148‑1 (MQ=255)
                                                                                                                       aCGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcat                        >  2:3380394/1‑148 (MQ=255)
                                                                                                                        cGGTATGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcatt                       <  2:457616/148‑1 (MQ=255)
                                                                                                                            aTGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcattcatt                   <  1:309543/148‑1 (MQ=255)
                                                                                                                            aTGATTATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcattcatt                   >  1:2817115/1‑148 (MQ=255)
                                                                                                                                ttATCCAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTAc               >  2:989073/1‑148 (MQ=255)
                                                                                                                                     cAGTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTg          <  1:2168390/148‑1 (MQ=255)
                                                                                                                                       gTTTGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTggg        <  1:800526/148‑1 (MQ=255)
                                                                                                                                          tGCGGACTCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACTAGTGGGAGt     <  1:941574/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAA  >  CP009273/1350746‑1351034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑