Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,412,385 G→T 100% N34K (AAC→AAA)  kilR ← Rac prophage; inhibitor of ftsZ, killing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,412,3850GT95.5% 662.3 / 19.3 223N34K (AAC→AAA) kilRRac prophage; inhibitor of ftsZ, killing protein
Reads supporting (aligned to +/- strand):  ref base G (3/7);  new base T (119/94);  total (122/101)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 228 total aligned reads displayed.

TAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTC  >  CP009273/1412240‑1412529
                                                                                                                                                 |                                                                                                                                                
tAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATtttt                                                                                                                                                >  1:1627425/1‑148 (MQ=255)
tAAGCAAATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATtttt                                                                                                                                                <  2:148760/148‑1 (MQ=255)
      caTTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGa                                                                                                                                          >  1:1582161/1‑148 (MQ=255)
           taatttCGTAGTCCTCACCATGACTCCGCCTGTACAGGTAACCCATCACGCCCGAGGCAGACTTTAATCCTGCGGTCAGTAAGGACTGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCaa                                                                                                                                     <  1:2638521/145‑1 (MQ=255)
             ttttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAata                                                                                                                                   >  2:1894985/1‑148 (MQ=255)
              tttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAatat                                                                                                                                  <  2:3427255/148‑1 (MQ=255)
              tttCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAatat                                                                                                                                  <  2:1284152/148‑1 (MQ=255)
                  ttAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGt                                                                                                                              <  2:490880/148‑1 (MQ=255)
                  ttAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGt                                                                                                                              <  2:2858756/148‑1 (MQ=255)
                  ttAGTAATCACCATTTCTCCTCATTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTTTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGACGAGGCAATATATGt                                                                                                                              <  1:2270019/148‑1 (MQ=255)
                     gTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACTACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCg                                                                                                                           >  1:1825714/1‑148 (MQ=255)
                          caCCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGACTGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCtt                                                                                                                      >  2:767589/1‑148 (MQ=255)
                               tGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATga                                                                                                                 >  2:178569/1‑148 (MQ=255)
                               tGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATga                                                                                                                 <  2:2176581/148‑1 (MQ=255)
                                taCTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaa                                                                                                                >  2:1474010/2‑148 (MQ=255)
                                gACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaa                                                                                                                >  1:1342585/1‑148 (MQ=255)
                                 aCTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCACTATATGTCCGGCCTTCATgaag                                                                                                               >  2:640547/1‑148 (MQ=255)
                                 aCTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaag                                                                                                               <  2:894856/148‑1 (MQ=255)
                                  cTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaaga                                                                                                              <  2:2651110/148‑1 (MQ=255)
                                  cTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaaga                                                                                                              >  2:1317744/1‑148 (MQ=255)
                                  cTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaaga                                                                                                              >  1:1727060/1‑148 (MQ=255)
                                  cTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaaga                                                                                                              >  1:3115388/1‑148 (MQ=255)
                                  atCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaaga                                                                                                              >  1:3486705/2‑148 (MQ=255)
                                   tCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATgaagaa                                                                                                             >  1:1193648/1‑148 (MQ=255)
                                     cGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACa                                                                                                           <  2:2393700/148‑1 (MQ=255)
                                        cTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATaa                                                                                                        >  2:3040828/1‑148 (MQ=255)
                                         tttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAAt                                                                                                       >  2:1551979/1‑148 (MQ=255)
                                         tttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAAt                                                                                                       >  1:2063713/1‑148 (MQ=255)
                                         tttACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAAt                                                                                                       <  1:3267599/148‑1 (MQ=255)
                                                gTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAgg                                                                                                >  1:93100/1‑148 (MQ=255)
                                                     ccATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTca                                                                                           <  2:3297623/148‑1 (MQ=255)
                                                       aTCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTcaca                                                                                         >  1:2529604/1‑148 (MQ=255)
                                                         cACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTcacaca                                                                                       <  2:1103164/148‑1 (MQ=255)
                                                           cGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACt                                                                                     >  2:3129356/1‑148 (MQ=255)
                                                            gACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTg                                                                                    <  2:917126/148‑1 (MQ=255)
                                                             aCCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGa                                                                                   <  2:2027138/148‑1 (MQ=255)
                                                                  ggAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTgg                                                                              >  1:1015352/1‑148 (MQ=255)
                                                                  ggAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTgg                                                                              >  2:3181226/1‑148 (MQ=255)
                                                                  ggAAGAATTTAATCATGCGGTCATTAATTAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGAAATATATGTCCGGCATTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTgg                                                                              <  1:624602/148‑1 (MQ=255)
                                                                     aGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTAt                                                                           >  2:2908951/1‑148 (MQ=255)
                                                                          ttAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtcat                                                                      >  2:339565/1‑148 (MQ=255)
                                                                            aaTCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTtcatca                                                                    <  1:1377948/148‑1 (MQ=255)
                                                                               cATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGtt                                                                 <  1:1648553/148‑1 (MQ=255)
                                                                                  gCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCg                                                              >  1:579090/1‑148 (MQ=255)
                                                                                     gTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAg                                                           <  1:3292318/148‑1 (MQ=255)
                                                                                     gTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAg                                                           >  1:2181177/1‑148 (MQ=255)
                                                                                        aGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGa                                                        >  2:3330984/1‑148 (MQ=255)
                                                                                         gTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAt                                                       >  1:2798803/1‑148 (MQ=255)
                                                                                          tAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAtg                                                      >  2:912875/1‑148 (MQ=255)
                                                                                            atgaatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGAtgtg                                                    >  2:3222201/1‑148 (MQ=255)
                                                                                             tgaatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGc                                                   >  2:350820/1‑148 (MQ=255)
                                                                                             tgaatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGc                                                   >  2:350408/1‑148 (MQ=255)
                                                                                              gaatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCa                                                  >  1:1741762/1‑148 (MQ=255)
                                                                                               aatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGTTATTTCATCAGTTCCGAAGTGATGTGCaa                                                 >  2:2879525/1‑148 (MQ=255)
                                                                                               aatgTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCaa                                                 <  1:1914539/148‑1 (MQ=255)
                                                                                                   tttttGTGGTCAGGTTACTAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCAt                                             <  1:3353755/148‑1 (MQ=255)
                                                                                                   tttttGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCAt                                             >  1:3145383/1‑148 (MQ=255)
                                                                                                        gtgGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTa                                        <  1:1983632/148‑1 (MQ=255)
                                                                                                        gtgGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATAGGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTa                                        <  1:447578/148‑1 (MQ=255)
                                                                                                           gTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTAtct                                     <  2:2365463/148‑1 (MQ=255)
                                                                                                            tCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTAtctc                                    <  2:1727926/148‑1 (MQ=255)
                                                                                                             cAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTcc                                   >  1:2598520/1‑148 (MQ=255)
                                                                                                             cAGGTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTcc                                   <  2:812250/148‑1 (MQ=255)
                                                                                                                gTTACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGTGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAtt                                >  1:1388194/1‑148 (MQ=255)
                                                                                                                 ttACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAttt                               >  1:2468636/1‑148 (MQ=255)
                                                                                                                  tACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAtttt                              >  1:2447767/1‑148 (MQ=255)
                                                                                                                  tACGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCAtttt                              >  2:1880601/1‑148 (MQ=255)
                                                                                                                    cGAATATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTa                            <  2:1582161/148‑1 (MQ=255)
                                                                                                                    cGAATATATAGTTTTCGATTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTa                            <  2:26551/148‑1 (MQ=255)
                                                                                                                      aaTATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACa                          >  1:964056/1‑148 (MQ=255)
                                                                                                                      aaTATATAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACa                          >  2:1323358/1‑148 (MQ=255)
                                                                                                                       atatatAGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACaa                         >  1:829601/1‑148 (MQ=255)
                                                                                                                         atataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAAt                       >  2:2494452/1‑148 (MQ=255)
                                                                                                                         atataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAAt                       <  2:1105348/148‑1 (MQ=255)
                                                                                                                         atataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAAt                       <  2:2837098/148‑1 (MQ=255)
                                                                                                                          tataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATg                      >  2:624895/1‑148 (MQ=255)
                                                                                                                           ataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGa                     >  1:1112630/1‑148 (MQ=255)
                                                                                                                           ataGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGa                     <  2:133818/148‑1 (MQ=255)
                                                                                                                            taGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGaa                    <  1:3222201/148‑1 (MQ=255)
                                                                                                                            taGTTTTCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGaa                    <  1:1928277/148‑1 (MQ=255)
                                                                                                                               ttttCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACtt                 <  2:3071099/148‑1 (MQ=255)
                                                                                                                               ttttCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACtt                 >  2:1747682/1‑148 (MQ=255)
                                                                                                                                tttCGCTTTTTAATATTTTTTTCCGAGGCAATATATGTCCGGCCATCAATAATAACATAAACGCCAGGAGTCACACACTGAAGTTGTATTTCATAATATCCGAAGTGATGTTCAAACATAATTATCTCCATTTTTAAAAAAGAACatt                >  1:2774794/1‑148 (MQ=255)
                                                                                                                                 ttCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttg               <  1:2981751/148‑1 (MQ=255)
                                                                                                                                 ttCGATTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttg               <  1:798699/148‑1 (MQ=255)
                                                                                                                                  tCGCTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttgt              >  2:1390612/1‑148 (MQ=255)
                                                                                                                                  tCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTttgt              <  1:3373163/148‑1 (MQ=255)
                                                                                                                                     cTTTTTAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGa           <  1:178569/148‑1 (MQ=255)
                                                                                                                                       ttttAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATg         <  1:2518259/148‑1 (MQ=255)
                                                                                                                                       ttttAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATg         <  1:3555797/148‑1 (MQ=255)
                                                                                                                                        tttAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACCTAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATTATTATCTCCATTTTTACAAATGAACTTTGTTGATGc        <  2:1825714/148‑1 (MQ=255)
                                                                                                                                         ttAATATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCg       >  1:2221899/1‑148 (MQ=255)
                                                                                                                                           aaTATTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgt     >  1:2149535/1‑148 (MQ=255)
                                                                                                                                            atatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtg    >  1:1079418/1‑148 (MQ=255)
                                                                                                                                             tatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtgt   >  2:385009/1‑148 (MQ=255)
                                                                                                                                             tatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtgt   <  2:516005/148‑1 (MQ=255)
                                                                                                                                             tatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtgt   >  1:909907/1‑148 (MQ=255)
                                                                                                                                             tatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtgt   >  1:1277996/1‑148 (MQ=255)
                                                                                                                                             tatTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGgtgt   >  2:257010/1‑148 (MQ=255)
                                                                                                                                              atTTTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTc  >  1:1995362/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAAGCACATTTTATTTTCTTAGTCATCACCATGACTCCGCCTTTACAGGTAAACCATCACGACCGAGGAAGACTTTAATCATGCGGTCAGTAATGAATGTTTTTGTGGTCAGGTTACGAATATATAGTTTTCGCTTTTTAATATTGTTTGCCGAGGCAATATATGTCCGGCCTTCATGAAGAACATAATCGCCAGGAGTCACACACTGACGTGGTATTTCATCAGTTCCGAAGTGATGTGCAATCATAATTATCTCCATTTTTACAAATGAACTTTGTTGATGCGGTGTC  >  CP009273/1412240‑1412529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑