Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,071 G→T 100% I214I (ATC→ATA)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,0710GT96.5% 701.5 / 13.1 225I214I (ATC→ATA) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (2/6);  new base T (100/117);  total (102/123)
Fisher's exact test for biased strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 226 total aligned reads displayed.

CGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGA  >  CP009273/136926‑137218
                                                                                                                                                 |                                                                                                                                                   
cGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAt                                                                                                                                                   <  1:843963/148‑1 (MQ=255)
    cTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCa                                                                                                                                               >  1:1284581/1‑148 (MQ=255)
         aaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCgg                                                                                                                                          >  2:1335565/1‑148 (MQ=255)
         aaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCgg                                                                                                                                          <  2:1512005/148‑1 (MQ=255)
           gACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGAt                                                                                                                                        >  2:1049939/1‑148 (MQ=255)
             cTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCg                                                                                                                                      >  1:3242773/1‑148 (MQ=255)
               cGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGtt                                                                                                                                    <  1:2914573/148‑1 (MQ=255)
               cGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGtt                                                                                                                                    >  2:357336/1‑148 (MQ=255)
                  tGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCgg                                                                                                                                 <  1:105840/148‑1 (MQ=255)
                   ggTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgc                                                                                                                                >  2:16293/1‑148 (MQ=255)
                      tttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgctgc                                                                                                                             <  1:487253/148‑1 (MQ=255)
                      tttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGgctgc                                                                                                                             <  2:2329577/148‑1 (MQ=255)
                         cAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTc                                                                                                                          <  2:828376/148‑1 (MQ=255)
                         cAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTc                                                                                                                          <  1:3468025/148‑1 (MQ=255)
                           gCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcac                                                                                                                        >  1:1567423/1‑148 (MQ=255)
                                  aTCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCGTCACCTCGccc                                                                                                                 >  2:3346276/1‑147 (MQ=255)
                                    cGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGt                                                                                                               >  1:922317/1‑148 (MQ=255)
                                    cGTAATGCCATTTCTCTGTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGt                                                                                                               <  2:2689690/148‑1 (MQ=255)
                                      tAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTAc                                                                                                             >  2:2700859/1‑148 (MQ=255)
                                       aaTGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACg                                                                                                            >  2:1690210/1‑148 (MQ=255)
                                       aaTGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACg                                                                                                            >  2:3326941/1‑148 (MQ=255)
                                        aTGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACgg                                                                                                           >  1:211438/1‑148 (MQ=255)
                                         tGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGa                                                                                                          <  1:2250379/148‑1 (MQ=255)
                                          gCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGaa                                                                                                         >  1:2091209/1‑148 (MQ=255)
                                          gCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGaa                                                                                                         <  1:657410/148‑1 (MQ=255)
                                           ccATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAac                                                                                                        >  2:3153690/1‑148 (MQ=255)
                                           ccATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAac                                                                                                        <  2:529012/148‑1 (MQ=255)
                                           ccATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAac                                                                                                        <  2:1501562/148‑1 (MQ=255)
                                             aTTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACATGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAacac                                                                                                      >  1:3188009/1‑148 (MQ=255)
                                              tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                     >  2:101562/1‑148 (MQ=255)
                                               ttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAccc                                                                                                    >  2:2687744/1‑148 (MQ=255)
                                               ttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAccc                                                                                                    >  2:2132770/1‑148 (MQ=255)
                                               ttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAccc                                                                                                    <  2:3031085/148‑1 (MQ=255)
                                                tctcTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCa                                                                                                   >  2:1564279/1‑148 (MQ=255)
                                                tctcTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCa                                                                                                   >  2:2167976/1‑148 (MQ=255)
                                                    tttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGct                                                                                               >  1:454673/1‑148 (MQ=255)
                                                     ttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGctt                                                                                              >  1:1868009/1‑148 (MQ=255)
                                                       gCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttct                                                                                            <  1:716433/148‑1 (MQ=255)
                                                         cGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCttt                                                                                          >  2:701864/1‑148 (MQ=255)
                                                         cGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCttt                                                                                          >  2:700662/1‑148 (MQ=255)
                                                          gCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTc                                                                                         >  1:202495/1‑148 (MQ=255)
                                                              gcggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAt                                                                                     <  2:1750753/148‑1 (MQ=255)
                                                              gcggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAt                                                                                     >  2:2331919/1‑148 (MQ=255)
                                                               cggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAtt                                                                                    <  2:2566529/148‑1 (MQ=255)
                                                               cggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAtt                                                                                    <  2:2603498/148‑1 (MQ=255)
                                                                 gcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTAt                                                                                  >  1:4443/1‑148 (MQ=255)
                                                                 gcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTAt                                                                                  <  1:1530745/148‑1 (MQ=255)
                                                                 gcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTAt                                                                                  <  1:1094051/148‑1 (MQ=255)
                                                                  cATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATg                                                                                 >  2:186907/1‑148 (MQ=255)
                                                                    tCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGa                                                                               >  1:177386/1‑148 (MQ=255)
                                                                        gcgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGtt                                                                           >  1:2236137/1‑148 (MQ=255)
                                                                        gcgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGtt                                                                           <  1:2716523/148‑1 (MQ=255)
                                                                         cgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTa                                                                          <  2:386794/148‑1 (MQ=255)
                                                                              aCAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGc                                                                     <  2:2494055/148‑1 (MQ=255)
                                                                              aCAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGc                                                                     <  1:3225286/148‑1 (MQ=255)
                                                                              aCAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGc                                                                     >  2:723138/1‑148 (MQ=255)
                                                                               cAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCg                                                                    <  2:3309137/148‑1 (MQ=255)
                                                                                     cTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAAtt                                                              <  2:3271283/148‑1 (MQ=255)
                                                                                       ggTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTg                                                            <  2:2837765/148‑1 (MQ=255)
                                                                                       ggTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTg                                                            <  1:2531641/148‑1 (MQ=255)
                                                                                       ggTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTg                                                            <  1:1199772/148‑1 (MQ=255)
                                                                                        gTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGt                                                           <  1:2948899/148‑1 (MQ=255)
                                                                                        gTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGt                                                           >  1:3247999/1‑148 (MQ=255)
                                                                                         tGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGtt                                                          <  1:2454726/148‑1 (MQ=255)
                                                                                          gAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGttt                                                         <  2:2256843/148‑1 (MQ=255)
                                                                                          gAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGttt                                                         <  2:2660329/148‑1 (MQ=255)
                                                                                           aGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTc                                                        <  2:2738601/148‑1 (MQ=255)
                                                                                             cGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAg                                                      >  1:1708039/1‑148 (MQ=255)
                                                                                                 acacAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcggc                                                  >  2:64498/1‑148 (MQ=255)
                                                                                                     aGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGaaa                                              <  1:2808794/148‑1 (MQ=255)
                                                                                                        tAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGc                                           >  1:1783001/1‑148 (MQ=255)
                                                                                                         aaaGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCg                                          <  2:1566766/148‑1 (MQ=255)
                                                                                                           aGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGtt                                        >  1:2612458/1‑148 (MQ=255)
                                                                                                           aGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGtt                                        >  1:2286638/1‑148 (MQ=255)
                                                                                                            gggTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTTTTTCAGCGGCGAAAAGCGTTg                                       >  1:1002152/1‑148 (MQ=255)
                                                                                                             ggTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGa                                      <  1:519630/148‑1 (MQ=255)
                                                                                                              gtgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAc                                     >  2:1143633/1‑148 (MQ=255)
                                                                                                              gtgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAc                                     >  2:125172/1‑148 (MQ=255)
                                                                                                               tgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAcc                                    <  2:420059/148‑1 (MQ=255)
                                                                                                                    cccACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTGAGCGGCGCAAATCGGTGTCCTTcat                               >  1:16370/1‑146 (MQ=255)
                                                                                                                     ccACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTg                              >  2:2505167/1‑148 (MQ=255)
                                                                                                                      cACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGa                             >  1:3413921/1‑148 (MQ=255)
                                                                                                                       aCTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGAt                            <  2:800431/148‑1 (MQ=255)
                                                                                                                        cTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGAtt                           <  1:3301158/148‑1 (MQ=255)
                                                                                                                        cTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGAtt                           <  1:1141808/148‑1 (MQ=255)
                                                                                                                         tttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCGAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTa                          >  2:1576083/1‑148 (MQ=255)
                                                                                                                         tttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTa                          <  2:3188015/148‑1 (MQ=255)
                                                                                                                          ttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAATCGGCGAAAAGCGTTGAACTTACttattaa                         >  1:3053939/1‑147 (MQ=255)
                                                                                                                           tAATCGGCGTCACTTCATTGGTTATTTCACCCTGATAGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGTCTTCTTTCAGATTATTGACGTTATCGGAGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACg                        <  1:1577397/148‑1 (MQ=255)
                                                                                                                               cGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCa                    <  1:1180970/148‑1 (MQ=255)
                                                                                                                               cGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCa                    <  1:2876556/148‑1 (MQ=255)
                                                                                                                               cGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCa                    <  1:2504819/148‑1 (MQ=255)
                                                                                                                                ggCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCAt                   <  2:1495934/148‑1 (MQ=255)
                                                                                                                                  cGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATag                 >  2:784104/1‑148 (MQ=255)
                                                                                                                                    tCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggc               <  1:1015895/148‑1 (MQ=255)
                                                                                                                                      aCTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggcag             >  2:159231/1‑148 (MQ=255)
                                                                                                                                          cATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCa         <  2:2236137/148‑1 (MQ=255)
                                                                                                                                             tGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTc      <  1:3200188/148‑1 (MQ=255)
                                                                                                                                             tGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTc      <  1:1433539/148‑1 (MQ=255)
                                                                                                                                              ggTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTcc     <  1:3553596/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGA  >  CP009273/136926‑137218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑