Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,508,681 C→A 100% F198L (TTC→TTA)  ydcU → putative spermidine/putrescine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,508,6810CA96.1% 613.9 / 13.2 204F198L (TTC→TTA) ydcUputative spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (2/6);  new base A (91/104);  total (93/111)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 204 total aligned reads displayed.

ATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATC  >  CP009273/1508536‑1508825
                                                                                                                                                 |                                                                                                                                                
aTGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAAt                                                                                                                                                >  2:685399/1‑148 (MQ=255)
   gCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGAt                                                                                                                                             <  2:2542679/148‑1 (MQ=255)
   gCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGAt                                                                                                                                             <  2:2355721/148‑1 (MQ=255)
   gCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGAt                                                                                                                                             <  2:1342894/148‑1 (MQ=255)
   gCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGAt                                                                                                                                             <  2:3079223/148‑1 (MQ=255)
     gTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATcc                                                                                                                                           <  1:471735/148‑1 (MQ=255)
     gTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATcc                                                                                                                                           <  1:1958933/148‑1 (MQ=255)
      tGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTTCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCt                                                                                                                                          >  2:3148388/1‑148 (MQ=255)
      tGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCt                                                                                                                                          >  2:1375873/1‑148 (MQ=255)
       ggCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTg                                                                                                                                         <  2:42665/148‑1 (MQ=255)
       ggCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTg                                                                                                                                         >  1:3078983/1‑148 (MQ=255)
        gCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGc                                                                                                                                        <  1:822290/148‑1 (MQ=255)
         cTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGcc                                                                                                                                       <  1:2499953/148‑1 (MQ=255)
              tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTc                                                                                                                                  <  1:103137/148‑1 (MQ=255)
              tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTc                                                                                                                                  <  1:2192812/148‑1 (MQ=255)
              tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                  <  2:1797764/148‑1 (MQ=255)
              tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                  >  2:2233390/1‑148 (MQ=255)
              tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                  >  1:1763976/1‑148 (MQ=255)
              tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                  >  2:2525564/1‑148 (MQ=255)
                 tttttACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATGACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAgg                                                                                                                               <  2:786894/148‑1 (MQ=255)
                  ttttACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAggc                                                                                                                              >  2:249917/1‑148 (MQ=255)
                       caacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGgcgc                                                                                                                         <  1:1243255/148‑1 (MQ=255)
                       caacaTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGgcgc                                                                                                                         <  1:2988733/148‑1 (MQ=255)
                            tCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAg                                                                                                                    >  1:2352488/1‑148 (MQ=255)
                               tGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGt                                                                                                                 <  2:2097829/148‑1 (MQ=255)
                               tGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGt                                                                                                                 <  2:2341309/148‑1 (MQ=255)
                               tGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGt                                                                                                                 <  2:252614/148‑1 (MQ=255)
                               tGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGt                                                                                                                 <  1:2920393/148‑1 (MQ=255)
                                 ggCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGttt                                                                                                               <  1:142354/148‑1 (MQ=255)
                                   cTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgc                                                                                                             <  2:2613627/148‑1 (MQ=255)
                                      gAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgccgc                                                                                                          >  2:3509557/1‑148 (MQ=255)
                                       aaCCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTgccgcc                                                                                                         >  2:2364493/1‑148 (MQ=255)
                                         ccACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGt                                                                                                       >  2:1285378/1‑148 (MQ=255)
                                         ccACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGt                                                                                                       <  1:1668613/148‑1 (MQ=255)
                                          cACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTc                                                                                                      >  1:763901/1‑148 (MQ=255)
                                            ctgctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAt                                                                                                    <  2:1852709/148‑1 (MQ=255)
                                             tgctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAtt                                                                                                   <  1:2314685/148‑1 (MQ=255)
                                               ctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgt                                                                                                 >  1:278968/1‑148 (MQ=255)
                                                tgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgtt                                                                                                <  2:2051369/148‑1 (MQ=255)
                                                 gACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgttg                                                                                               >  2:2886774/1‑148 (MQ=255)
                                                  aCTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGc                                                                                              <  1:2962381/148‑1 (MQ=255)
                                                  aCTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGc                                                                                              <  1:2552405/148‑1 (MQ=255)
                                                   cTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCa                                                                                             <  1:3507618/148‑1 (MQ=255)
                                                     gCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAgg                                                                                           >  1:1749529/1‑148 (MQ=255)
                                                      cGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGc                                                                                          >  2:1047668/1‑148 (MQ=255)
                                                        ttCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGt                                                                                        >  1:1586170/1‑148 (MQ=255)
                                                           cTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCgg                                                                                     <  2:2836297/148‑1 (MQ=255)
                                                            ttACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGc                                                                                    <  2:932907/148‑1 (MQ=255)
                                                            ttACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGc                                                                                    >  1:2365618/1‑148 (MQ=255)
                                                               cATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGa                                                                                 >  1:3243776/1‑148 (MQ=255)
                                                               cATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGGTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGa                                                                                 >  1:3056754/1‑148 (MQ=255)
                                                               cATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCGTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGa                                                                                 >  1:2797848/1‑148 (MQ=255)
                                                                aTTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGAt                                                                                <  2:537131/148‑1 (MQ=255)
                                                                       gCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGgcg                                                                         >  2:1685683/1‑148 (MQ=255)
                                                                              gCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTcc                                                                  >  2:821015/1‑148 (MQ=255)
                                                                               cGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGGTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCa                                                                 >  1:3171082/1‑148 (MQ=255)
                                                                                    aTACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACa                                                            >  2:3259773/1‑148 (MQ=255)
                                                                                          tGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCtt                                                      <  2:3316131/148‑1 (MQ=255)
                                                                                           gTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCttt                                                     <  2:1763976/148‑1 (MQ=255)
                                                                                           gTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCttt                                                     <  1:220777/148‑1 (MQ=255)
                                                                                           gTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCttt                                                     <  2:1585561/148‑1 (MQ=255)
                                                                                                cTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTa                                                >  2:2607699/1‑148 (MQ=255)
                                                                                                  tCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTAtg                                              >  1:2875343/1‑148 (MQ=255)
                                                                                                       gCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTAGgtggtg                                         >  2:2033309/1‑148 (MQ=255)
                                                                                                          ggggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctg                                      <  2:2715977/148‑1 (MQ=255)
                                                                                                          ggggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctg                                      <  2:1311206/148‑1 (MQ=255)
                                                                                                             gcgcTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                   <  2:1675715/148‑1 (MQ=255)
                                                                                                             gcgcTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                   <  1:1325782/148‑1 (MQ=255)
                                                                                                             gcgcTGTCTGGTGTTTCTCTAGATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                   <  2:1888055/148‑1 (MQ=255)
                                                                                                              cgcTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGc                                  <  1:2886774/148‑1 (MQ=255)
                                                                                                                 tttCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTgg                               >  1:3467011/1‑148 (MQ=255)
                                                                                                                  ttCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGc                              >  2:2368086/1‑148 (MQ=255)
                                                                                                                   tCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCa                             <  1:821015/148‑1 (MQ=255)
                                                                                                                     tGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAAt                           <  2:522380/148‑1 (MQ=255)
                                                                                                                       gtgtTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATcc                         >  1:38199/1‑148 (MQ=255)
                                                                                                                        tgtTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATccc                        <  2:1583954/148‑1 (MQ=255)
                                                                                                                          tttCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCgg                      >  2:497101/1‑148 (MQ=255)
                                                                                                                           ttCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCggg                     >  2:471182/1‑148 (MQ=255)
                                                                                                                           ttCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCggg                     >  2:471130/1‑148 (MQ=255)
                                                                                                                           ttCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCggg                     >  2:470987/1‑148 (MQ=255)
                                                                                                                                 atttCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGGTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGc               <  1:2090033/145‑1 (MQ=255)
                                                                                                                                 atatCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGc               >  2:2621128/1‑148 (MQ=255)
                                                                                                                                   atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg             <  1:1110421/148‑1 (MQ=255)
                                                                                                                                   atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg             <  1:1110788/148‑1 (MQ=255)
                                                                                                                                   atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg             >  1:1066875/1‑148 (MQ=255)
                                                                                                                                   atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg             <  2:2989631/148‑1 (MQ=255)
                                                                                                                                   atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg             <  2:2776893/148‑1 (MQ=255)
                                                                                                                                   atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg             <  2:1217734/148‑1 (MQ=255)
                                                                                                                                     cTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCt           >  2:586590/1‑148 (MQ=255)
                                                                                                                                       ggTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTgg         <  2:3527799/148‑1 (MQ=255)
                                                                                                                                       ggTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTgg         >  1:2113739/1‑148 (MQ=255)
                                                                                                                                       ggTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTgg         <  2:225871/148‑1 (MQ=255)
                                                                                                                                        gTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGc        >  1:1779557/1‑148 (MQ=255)
                                                                                                                                        gTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGc        >  1:2552843/1‑148 (MQ=255)
                                                                                                                                         ttGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGct       <  1:435802/148‑1 (MQ=255)
                                                                                                                                            ccGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTa    <  2:38199/148‑1 (MQ=255)
                                                                                                                                            ccGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTa    >  1:2616464/1‑148 (MQ=255)
                                                                                                                                             cGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTAt   <  1:220827/148‑1 (MQ=255)
                                                                                                                                             cGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTAt   >  1:3417501/1‑148 (MQ=255)
                                                                                                                                              gTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATc  <  2:3467011/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATGGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATC  >  CP009273/1508536‑1508825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑