Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,508,684 G→T 7.0% M199I (ATG→ATT)  ydcU → putative spermidine/putrescine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,508,6840GT 7.0% 769.5 / 18.6 215M199I (ATG→ATT) ydcUputative spermidine/putrescine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (94/104);  new base T (8/9);  total (102/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

GGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTAC  >  CP009273/1508538‑1508830
                                                                                                                                                  |                                                                                                                                                  
ggggTGGCTCATTTGTTTTTACAACATCATGGGGTTGTAACACTGCTGGCTGCGTTCCTTAACTTTCCTGCGGTGGGGGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGa                                                                                                                                                   <  2:2234574/145‑1 (MQ=255)
ggCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGa                                                                                                                                                   <  2:445912/148‑1 (MQ=255)
 gCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGAt                                                                                                                                                  <  2:1342894/148‑1 (MQ=255)
 gCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGAt                                                                                                                                                  <  2:2542679/148‑1 (MQ=255)
   gTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATcc                                                                                                                                                <  1:2837143/148‑1 (MQ=255)
   gTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATcc                                                                                                                                                <  1:471204/148‑1 (MQ=255)
   gTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATcc                                                                                                                                                <  1:1958934/148‑1 (MQ=255)
    tGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCt                                                                                                                                               >  2:2121865/1‑148 (MQ=255)
     ggCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTg                                                                                                                                              <  1:1484191/148‑1 (MQ=255)
      gCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGc                                                                                                                                             <  1:822290/148‑1 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTc                                                                                                                                       <  1:103137/148‑1 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTc                                                                                                                                       <  1:2192812/148‑1 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                       >  1:1763978/1‑148 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                       <  2:1797764/148‑1 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                       >  1:1763804/1‑148 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                       >  1:262674/1‑148 (MQ=255)
            tGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTc                                                                                                                                       >  2:2525564/1‑148 (MQ=255)
                ttttACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAggc                                                                                                                                   >  2:249917/1‑148 (MQ=255)
                         aTCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGa                                                                                                                          <  2:2360648/148‑1 (MQ=255)
                          tCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAg                                                                                                                         >  1:2339665/1‑148 (MQ=255)
                             tGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGt                                                                                                                      <  2:252614/148‑1 (MQ=255)
                             tGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGt                                                                                                                      >  2:520728/1‑148 (MQ=255)
                              gggCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGtt                                                                                                                     <  1:2547153/148‑1 (MQ=255)
                                      aCCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCGGGTGTTTCTGTATAGGTGGTTGCCGTTAATTAAACTGCCCGTTCAGGCGGCGCTTGAGCGTTTGgcgccg                                                                                                             >  1:1607426/1‑148 (MQ=255)
                                       ccACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGt                                                                                                            <  2:3505972/148‑1 (MQ=255)
                                       ccACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGt                                                                                                            >  2:1285378/1‑148 (MQ=255)
                                        cACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTc                                                                                                           <  2:2818748/148‑1 (MQ=255)
                                        cACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTc                                                                                                           >  1:763901/1‑148 (MQ=255)
                                          ctgctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAt                                                                                                         <  2:1852709/148‑1 (MQ=255)
                                             ctgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgt                                                                                                      >  1:278968/1‑148 (MQ=255)
                                              tgACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgtt                                                                                                     <  2:2052553/148‑1 (MQ=255)
                                               gACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCAttgttg                                                                                                    >  2:2886774/1‑148 (MQ=255)
                                                aCTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTTCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGc                                                                                                   <  1:2962301/148‑1 (MQ=255)
                                                aCTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGACGCCGTCATTGTTGc                                                                                                   >  1:2899559/1‑148 (MQ=255)
                                                aCTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGc                                                                                                   <  1:297886/148‑1 (MQ=255)
                                                 cTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCa                                                                                                  <  1:3507618/148‑1 (MQ=255)
                                                    cGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGc                                                                                               >  2:1047668/1‑148 (MQ=255)
                                                      ttCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGt                                                                                             <  2:2723352/148‑1 (MQ=255)
                                                       tCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTc                                                                                            >  2:3519333/1‑148 (MQ=255)
                                                         cTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCgg                                                                                          <  2:2836314/148‑1 (MQ=255)
                                                          ttACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGc                                                                                         <  2:932907/148‑1 (MQ=255)
                                                          ttACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGc                                                                                         <  2:2348728/148‑1 (MQ=255)
                                                          ttACATTACCTGCGGTGGGCGGAAAGACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATGGTTGCAGGCGTCGGc                                                                                         <  2:2707796/148‑1 (MQ=255)
                                                           tACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCt                                                                                        >  2:474299/1‑148 (MQ=255)
                                                              aTTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGAt                                                                                     <  2:537131/148‑1 (MQ=255)
                                                                  ccTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCg                                                                                 >  2:1541546/1‑148 (MQ=255)
                                                                    tGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCGTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGgc                                                                               >  1:2434596/1‑148 (MQ=255)
                                                                     gCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGgcg                                                                              >  2:1685683/1‑148 (MQ=255)
                                                                            gCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTcc                                                                       >  2:821015/1‑148 (MQ=255)
                                                                                  aTACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACa                                                                 >  2:3259704/1‑148 (MQ=255)
                                                                                         gTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCttt                                                          <  1:220777/148‑1 (MQ=255)
                                                                                         gTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCttt                                                          <  2:1763976/148‑1 (MQ=255)
                                                                                         gTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCttt                                                          <  2:1585513/148‑1 (MQ=255)
                                                                                           cAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCg                                                        <  2:1351481/148‑1 (MQ=255)
                                                                                             aCTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCt                                                      >  2:3102194/1‑148 (MQ=255)
                                                                                              cTTCCGGGCTGGGGCGCGTTGTGGTGTTTCTCTATATCTGGTTGCCGTTAATTATACTGCCCGTTCAGGCGGCGCTTGAGCGTTTGGCGCCGGCATTTTTTCAGGCGTCGGCTGATATCGGCGCACGGCCACCACAAAACATTCGCTa                                                     >  1:307567/1‑148 (MQ=255)
                                                                                               ttCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTAt                                                    <  1:185568/148‑1 (MQ=255)
                                                                                                     gCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTAGgtggtg                                              >  2:2033309/1‑148 (MQ=255)
                                                                                                        ggggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctg                                           <  2:2612946/148‑1 (MQ=255)
                                                                                                        ggggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctg                                           <  2:1311206/148‑1 (MQ=255)
                                                                                                        ggggCGCTTTCTGGTGTTTCTCGATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctg                                           <  2:2614368/148‑1 (MQ=255)
                                                                                                         gggCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctgc                                          >  2:3458295/1‑148 (MQ=255)
                                                                                                         gggCGCTTTCTGGTGTATCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGtgctgc                                          >  2:1133856/1‑148 (MQ=255)
                                                                                                           gcgcTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                        <  1:1325782/148‑1 (MQ=255)
                                                                                                           gcgcTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATACTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTTCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCATTCGCTATGTGGTGCTGCCg                                        >  1:3008532/1‑148 (MQ=255)
                                                                                                           gcgcTGTCTGGTGTTTCTCTAGATCTGGTTGCCGTTAATTATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                        <  2:1888055/148‑1 (MQ=255)
                                                                                                           gcgcGTTCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCg                                        >  2:1636001/1‑148 (MQ=255)
                                                                                                              cTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTg                                     <  2:2287212/148‑1 (MQ=255)
                                                                                                               tttCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTgg                                    >  1:3467011/1‑148 (MQ=255)
                                                                                                                ttCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCTCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGc                                   >  2:3285222/1‑148 (MQ=255)
                                                                                                                ttCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGc                                   >  2:2368024/1‑148 (MQ=255)
                                                                                                                 tCTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCa                                  <  1:821015/148‑1 (MQ=255)
                                                                                                                  cTGGTGTTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCaa                                 >  1:3431718/1‑148 (MQ=255)
                                                                                                                     gtgtTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATcc                              >  1:38199/1‑148 (MQ=255)
                                                                                                                     gtgtTTCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATcc                              <  1:2877915/148‑1 (MQ=255)
                                                                                                                         ttCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCggg                          >  2:471134/1‑148 (MQ=255)
                                                                                                                         ttCTCTATATCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCggg                          >  2:471129/1‑148 (MQ=255)
                                                                                                                              tagatCTGGTTGCCGATAAGTATCCGGCCCGTTCAGGCGGCGCTTGAGCGAGTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTg                     <  2:763873/145‑1 (MQ=255)
                                                                                                                                 atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg                  <  1:1110788/148‑1 (MQ=255)
                                                                                                                                 atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg                  <  1:1110421/148‑1 (MQ=255)
                                                                                                                                 atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg                  >  1:1066875/1‑148 (MQ=255)
                                                                                                                                 atCTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCg                  <  2:2989631/148‑1 (MQ=255)
                                                                                                                                  tCTGGTTGCCGTTTATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCAGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCTCGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGc                 <  1:331759/148‑1 (MQ=255)
                                                                                                                                   cTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCt                >  1:3216080/1‑148 (MQ=255)
                                                                                                                                   cTGGTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCt                >  2:586590/1‑148 (MQ=255)
                                                                                                                                     ggTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTgg              >  1:2113739/1‑148 (MQ=255)
                                                                                                                                      gTTGCCGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGc             >  1:2552744/1‑148 (MQ=255)
                                                                                                                                          ccGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTa         <  2:38214/148‑1 (MQ=255)
                                                                                                                                          ccGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTa         >  1:2613962/1‑148 (MQ=255)
                                                                                                                                          ccGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAAACCCGGGTATTGCCGCTGGCTCTa         >  1:1679591/1‑148 (MQ=255)
                                                                                                                                           cGTTAATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTAt        <  1:220827/148‑1 (MQ=255)
                                                                                                                                             ttaaTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCt      >  1:1156615/4‑148 (MQ=255)
                                                                                                                                             ttaaTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCt      >  2:2583361/4‑148 (MQ=255)
                                                                                                                                             ttaaTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCt      >  2:800985/4‑148 (MQ=255)
                                                                                                                                              taaTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCACTCCCGGGTCTTGCCGCTGGCtctctcgg     >  1:500002/3‑146 (MQ=255)
                                                                                                                                              taaTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCtt     >  2:362050/3‑148 (MQ=255)
                                                                                                                                              taaTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCtt     >  1:2298641/3‑148 (MQ=255)
                                                                                                                                               aaTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCttt    <  1:12526/147‑1 (MQ=255)
                                                                                                                                                aTGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTa   >  2:3305151/1‑148 (MQ=255)
                                                                                                                                                 tGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTAc  <  1:2364493/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCGTGGCTCAGTGGTTTTTACAACATCTTGGGCTGGAACCACTGCTGACTGCGTTCCTTACATTACCTGCGGTGGGCGGAAATACGCTGTCAACTTCCGGGCTGGGGCGCTTTCTGGTGTTTCTCTATATCTGGTTGCCGTTCATGATCCTGCCCGTTCAGGCGGCGCTTGAGCGTTTGCCGCCGTCATTGTTGCAGGCGTCGGCTGATCTCGGCGCACGTCCACGACAAACCTTTCGCTATGTGGTGCTGCCGCTGGCAATCCCGGGTATTGCCGCTGGCTCTATCTTTAC  >  CP009273/1508538‑1508830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑