| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,514,858 | G→A | 100% | E114K (GAA→AAA) | mcbR → | colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,514,858 | 0 | G | A | 95.7% | 820.1 / 20.8 | 253 | E114K (GAA→AAA) | mcbR | colanic acid and biofilm gene transcriptional regulator, MqsR‑controlled |
| Reads supporting (aligned to +/- strand): ref base G (8/3); new base A (90/152); total (98/155) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 255 total aligned reads displayed.
CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGA > CP009273/1514712‑1515002 | tGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGaaa < 2:3445535/148‑1 (MQ=255) tGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGaaa < 1:504021/148‑1 (MQ=255) gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACTAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGc > 2:1158343/1‑148 (MQ=255) gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGc < 2:3097070/148‑1 (MQ=255) gCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGc < 2:1327196/148‑1 (MQ=255) ccTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCg > 2:1162159/1‑148 (MQ=255) cTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCga < 1:3399356/148‑1 (MQ=255) tGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCgag < 1:3269394/148‑1 (MQ=255) acaAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGaaa < 2:3162984/148‑1 (MQ=255) caAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAAt > 1:1452763/1‑148 (MQ=255) aaGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAAtt < 1:1048518/148‑1 (MQ=255) tttACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAcagc < 2:1237674/148‑1 (MQ=255) tACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTAcagcag > 1:913024/1‑148 (MQ=255) ttCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCa < 2:3362155/148‑1 (MQ=255) ttCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCa > 1:2022439/1‑148 (MQ=255) tCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGATACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCaa < 2:601234/148‑1 (MQ=255) ggAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGAACTTGCGGAACTCCAGAAATTGATCGAGAAATTACAGCAGGCGCAAGaa > 1:2425416/1‑148 (MQ=255) gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaa < 1:641234/148‑1 (MQ=255) gAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGaaa < 2:1694081/148‑1 (MQ=255) gTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAgg > 1:254041/1‑148 (MQ=255) gTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAgg < 2:1984575/148‑1 (MQ=255) gTGGGGAAACGTCAATTAGACGAAATCAATCGGATCAGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAgg < 2:687752/148‑1 (MQ=255) tGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAggg > 2:1218943/1‑148 (MQ=255) ggggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGc < 1:3047882/148‑1 (MQ=255) ggAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGa > 2:2138507/1‑148 (MQ=255) gAAACGTCAATGAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGAc < 1:1863836/148‑1 (MQ=255) aCGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATg > 2:467351/1‑148 (MQ=255) cGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATgg > 1:833864/1‑148 (MQ=255) tCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGaa > 2:341465/1‑148 (MQ=255) aTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaa > 1:2789713/1‑148 (MQ=255) aTTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaa < 2:2647130/148‑1 (MQ=255) ttAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACaaa > 2:2854443/1‑148 (MQ=255) tAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAAt < 1:2192996/148‑1 (MQ=255) gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa < 1:1162159/148‑1 (MQ=255) gACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCa < 1:2201115/148‑1 (MQ=255) gAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTa < 1:2280366/148‑1 (MQ=255) aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa < 2:246138/148‑1 (MQ=255) aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa < 2:2247151/148‑1 (MQ=255) aaaTCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTaa < 2:1315189/148‑1 (MQ=255) aatcaatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAt < 1:611701/148‑1 (MQ=255) aatcaatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAAt < 2:1815634/148‑1 (MQ=255) caatcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTa < 2:531323/148‑1 (MQ=255) atcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTaaa < 1:1038912/148‑1 (MQ=255) tcGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAAc < 1:3002327/148‑1 (MQ=255) ggATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAg > 2:587175/1‑148 (MQ=255) ggATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAg > 2:887976/1‑148 (MQ=255) gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg > 2:2504265/1‑148 (MQ=255) gATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAgg < 2:1770012/148‑1 (MQ=255) aTCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGc < 1:1929654/148‑1 (MQ=255) ccGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTa < 1:443629/148‑1 (MQ=255) tcTACGAACTGGAATTAATTGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAtt < 2:3337810/147‑1 (MQ=255) gCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAtt < 1:157158/148‑1 (MQ=255) cTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTAttt < 1:1904266/148‑1 (MQ=255) aCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGATATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCg < 1:3421663/148‑1 (MQ=255) aCGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCg > 2:3320789/1‑148 (MQ=255) gAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAACAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCt < 2:2022448/148‑1 (MQ=255) gAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCt < 2:2737900/148‑1 (MQ=255) aaCTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCtt < 1:1339033/148‑1 (MQ=255) aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt > 2:3494359/1‑148 (MQ=255) aaTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAt > 1:3506624/1‑148 (MQ=255) aTTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAtt > 1:1254041/1‑148 (MQ=255) ttAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt > 1:2036916/1‑148 (MQ=255) ttAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGAttt < 2:1418795/148‑1 (MQ=255) tAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa < 2:1145178/148‑1 (MQ=255) tAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTa > 1:722268/1‑148 (MQ=255) tGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTatca < 1:290057/148‑1 (MQ=255) cAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCg < 2:867562/148‑1 (MQ=255) cAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCg < 2:2589440/148‑1 (MQ=255) aGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGc > 2:2413874/1‑148 (MQ=255) aGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCTCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGc > 2:3239975/1‑148 (MQ=255) aGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGc < 1:920998/148‑1 (MQ=255) ttGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTc > 1:1690969/1‑148 (MQ=255) cTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGcc < 2:1846316/148‑1 (MQ=255) tGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATc > 2:1968776/1‑148 (MQ=255) tGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATc > 2:176448/1‑148 (MQ=255) aaaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCt < 1:717557/148‑1 (MQ=255) aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg > 2:2015026/1‑148 (MQ=255) aaaCCTCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCtg < 1:2994502/148‑1 (MQ=255) tCACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTga < 1:896133/148‑1 (MQ=255) cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag < 2:3455814/148‑1 (MQ=255) cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag < 2:913024/148‑1 (MQ=255) cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag < 1:2333304/148‑1 (MQ=255) cACCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgag < 2:1694591/148‑1 (MQ=255) aCCCCGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTgaga > 2:25615/1‑148 (MQ=255) cccGCAAGACCTTGCGGAACTCCAGATATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatg < 2:73950/148‑1 (MQ=255) cGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAgatgat > 2:102655/1‑148 (MQ=255) cGCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTGATCATCGCTCAAATATGACCATCCTGTGTGAgatgat > 2:3294553/1‑148 (MQ=255) gCAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGAtt > 2:400676/1‑148 (MQ=255) cAAGACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTg < 2:1382822/148‑1 (MQ=255) gACCTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGc > 1:3297720/1‑148 (MQ=255) ccTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCaa > 2:1426768/1‑148 (MQ=255) cTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAAc > 2:1568576/1‑148 (MQ=255) cTTGCGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAAc > 2:2813847/1‑148 (MQ=255) cGGAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACtgtg < 1:1218943/148‑1 (MQ=255) gAACTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTggg < 2:1199301/148‑1 (MQ=255) aCTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTc < 2:1795642/148‑1 (MQ=255) cTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCa > 2:3141538/1‑148 (MQ=255) cTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCa > 1:773347/1‑148 (MQ=255) cTCCAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCa > 1:163271/1‑148 (MQ=255) cAGAAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGa < 2:841451/148‑1 (MQ=255) | CTGTCGCACCTGCACAAGCATTTACAGTTCCGGAAGTGGGGAAACGTCAATTAGACGAAATCAATCGGATCCGCTACGAACTGGAATTAATGGCAGTTGCTCTGGCTGTTGAAAACCTCACCCCGCAAGACCTTGCGGAACTCCAGGAATTGCTCGAGAAATTACAGCAGGCGCAAGAAAAGGGCGACATGGAACAAATCATTAATGTAAACAGGCTATTTCGCTTAGCGATTTATCATCGCTCAAATATGCCCATCCTGTGTGAGATGATTGAGCAACTGTGGGTCAGGA > CP009273/1514712‑1515002 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |