Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 142,460 T→A 100% F298Y (TTC→TAC)  yadE → putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273142,4600TA100.0% 663.8 / NA 200F298Y (TTC→TAC) yadEputative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (103/97);  total (103/97)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

TATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTC  >  CP009273/142315‑142604
                                                                                                                                                 |                                                                                                                                                
tATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAcc                                                                                                                                                <  2:3234386/148‑1 (MQ=255)
   ccTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGt                                                                                                                                             >  1:2194062/1‑148 (MQ=255)
    cTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTc                                                                                                                                            >  2:3177806/1‑148 (MQ=255)
    cTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTc                                                                                                                                            >  2:169085/1‑148 (MQ=255)
    cTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTc                                                                                                                                            <  1:2675956/148‑1 (MQ=255)
        gtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACAt                                                                                                                                        <  2:2665636/148‑1 (MQ=255)
        gtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACAt                                                                                                                                        >  1:1343194/1‑148 (MQ=255)
        gtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACAt                                                                                                                                        >  1:3113740/1‑148 (MQ=255)
        gtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTAACAGTCACAt                                                                                                                                        >  1:3272031/1‑148 (MQ=255)
         ttGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATa                                                                                                                                       >  1:2488033/1‑148 (MQ=255)
            aaaCAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATAccc                                                                                                                                    >  1:2075960/1‑148 (MQ=255)
              aCAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAt                                                                                                                                  >  1:1039567/1‑148 (MQ=255)
              aCAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAt                                                                                                                                  >  1:1609748/1‑148 (MQ=255)
               cAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtt                                                                                                                                 >  2:12371/1‑148 (MQ=255)
                aaTATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAttt                                                                                                                                <  1:3259362/148‑1 (MQ=255)
                aaTATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTAGCGATGTATTTGATTACCAGTCACATACCAAtat                                                                                                                                >  1:840439/1‑146 (MQ=255)
                 atatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtttt                                                                                                                               <  2:1899144/148‑1 (MQ=255)
                 atatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtttt                                                                                                                               <  1:3370145/148‑1 (MQ=255)
                 atatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtttt                                                                                                                               <  1:2930292/148‑1 (MQ=255)
                 atatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtttt                                                                                                                               >  2:3459136/1‑148 (MQ=255)
                          gAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCgg                                                                                                                      >  1:144993/1‑148 (MQ=255)
                          gAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCgg                                                                                                                      <  2:311274/148‑1 (MQ=255)
                          gAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAAAGAAATTAGCGATGTATTTTATTAACAGTCACATACCCATTTTTTGCATCgg                                                                                                                      >  1:2422728/1‑148 (MQ=255)
                          gAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTATGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCgg                                                                                                                      >  2:1474168/1‑148 (MQ=255)
                               cGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAACTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGa                                                                                                                 <  1:605278/148‑1 (MQ=255)
                                  cGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATgg                                                                                                              <  1:1474168/148‑1 (MQ=255)
                                     cGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                           >  2:2880903/1‑148 (MQ=255)
                                     cGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                           <  2:3492583/148‑1 (MQ=255)
                                     cGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                           >  2:2551410/1‑148 (MQ=255)
                                     cGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                           >  2:105640/1‑148 (MQ=255)
                                     cGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTa                                                                                                           >  1:1361347/1‑148 (MQ=255)
                                      gTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTAt                                                                                                          <  1:3013103/148‑1 (MQ=255)
                                       tttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATc                                                                                                         >  2:160985/1‑148 (MQ=255)
                                        ttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCg                                                                                                        <  2:2397070/148‑1 (MQ=255)
                                         tATTGTTACCGCACGCATCACACGTCACCCGCAGAAGTGGAACCCAAAAGCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGc                                                                                                       <  2:1303139/148‑1 (MQ=255)
                                          aTTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGcc                                                                                                      >  1:2306887/1‑148 (MQ=255)
                                            tGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTGTGAATCGGGTAGATGGTTATCGCCGa                                                                                                    >  2:184887/1‑148 (MQ=255)
                                               tACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTCGATGGTTATCGCCGAccc                                                                                                 >  1:311386/1‑148 (MQ=255)
                                                  cTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCata                                                                                              <  2:2006672/148‑1 (MQ=255)
                                                  cTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCata                                                                                              <  2:1979906/148‑1 (MQ=255)
                                                   tCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCatat                                                                                             <  1:3083714/148‑1 (MQ=255)
                                                    cACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATAtt                                                                                            <  1:2287206/148‑1 (MQ=255)
                                                     aCGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTa                                                                                           >  1:1284240/1‑148 (MQ=255)
                                                     aCGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTa                                                                                           >  2:3542961/1‑148 (MQ=255)
                                                     aCGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTa                                                                                           <  1:50818/148‑1 (MQ=255)
                                                         aTCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTa                                                                                           >  2:2657318/1‑144 (MQ=255)
                                                         aTCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTa                                                                                           <  1:2657318/144‑1 (MQ=255)
                                                           cAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGc                                                                                     <  1:1591335/148‑1 (MQ=255)
                                                             aaCGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCg                                                                                   >  2:595340/1‑148 (MQ=255)
                                                              aCGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAACTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCAGATTACTGAGCCGt                                                                                  >  2:2195729/1‑148 (MQ=255)
                                                               cGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGa                                                                                       >  2:304711/1‑142 (MQ=255)
                                                               cGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                 <  1:663219/148‑1 (MQ=255)
                                                               cGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTa                                                                                 <  2:1405332/148‑1 (MQ=255)
                                                                  aaCCCGCAGAAGTGGAACCCAAAATCGATGCAAATTATGAGCGATTATGAGCTTAACGAAATTCGCGATGTATAAGAAAACCAGTAACATACACATTTTTTGCATCGGGTATATGGTTATCGAAGACACATATTACTGAGCCGTAGTg                                                                              >  2:1619208/2‑148 (MQ=255)
                                                                   aCCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGa                                                                             <  1:2454786/148‑1 (MQ=255)
                                                                        cAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGcaca                                                                        <  1:370064/148‑1 (MQ=255)
                                                                         aGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACaa                                                                       >  2:2065226/1‑148 (MQ=255)
                                                                          gAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAat                                                                      >  2:427713/1‑148 (MQ=255)
                                                                           aaGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAAGTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAata                                                                     >  2:1363060/1‑148 (MQ=255)
                                                                              tGGAACCCAAAATCGCTGCAAATTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTc                                                                  <  2:2539534/148‑1 (MQ=255)
                                                                                gAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTg                                                                <  2:695920/148‑1 (MQ=255)
                                                                                  aCCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGtt                                                              <  2:1427552/148‑1 (MQ=255)
                                                                                  aCCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGtt                                                              <  2:501404/148‑1 (MQ=255)
                                                                                      aaaaTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAt                                                          <  2:2251654/148‑1 (MQ=255)
                                                                                        aaTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAttt                                                        >  1:2956904/1‑148 (MQ=255)
                                                                                          tCGCTTCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg                                                      <  2:3036334/148‑1 (MQ=255)
                                                                                           cGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTga                                                     >  1:1623044/1‑147 (MQ=255)
                                                                                              tGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACg                                                  <  1:1932015/148‑1 (MQ=255)
                                                                                               gCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGt                                                 >  1:3069555/1‑148 (MQ=255)
                                                                                               gCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGt                                                 <  2:2952918/148‑1 (MQ=255)
                                                                                                cAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGtt                                                <  1:2234694/148‑1 (MQ=255)
                                                                                                 aaTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTc                                               <  1:2519894/148‑1 (MQ=255)
                                                                                                   tttATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAc                                             >  1:1302050/1‑148 (MQ=255)
                                                                                                   tttATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAc                                             >  1:2298963/1‑148 (MQ=255)
                                                                                                    ttATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAcg                                            <  1:202258/148‑1 (MQ=255)
                                                                                                         aGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg                                       >  2:1285641/1‑148 (MQ=255)
                                                                                                         aGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATGACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg                                       <  2:2152980/148‑1 (MQ=255)
                                                                                                                   aGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCa                             >  1:2685584/1‑148 (MQ=255)
                                                                                                                    gCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCaa                            <  2:1685562/148‑1 (MQ=255)
                                                                                                                    gCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCaa                            >  1:685409/1‑148 (MQ=255)
                                                                                                                     cTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAt                           >  2:76431/1‑148 (MQ=255)
                                                                                                                      ttAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAtt                          <  2:2402712/148‑1 (MQ=255)
                                                                                                                          cGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAt                      >  1:2856544/1‑148 (MQ=255)
                                                                                                                          cGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAt                      >  1:1084966/1‑148 (MQ=255)
                                                                                                                            aaaTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTa                        >  1:1213007/1‑144 (MQ=255)
                                                                                                                            aaaTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATcc                    >  1:26000/1‑148 (MQ=255)
                                                                                                                               ttCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCa                 <  2:892521/148‑1 (MQ=255)
                                                                                                                                tCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCAt                <  1:3232369/148‑1 (MQ=255)
                                                                                                                                 cgcgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATg               >  2:664872/1‑148 (MQ=255)
                                                                                                                                   cgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTc             >  1:3202558/1‑148 (MQ=255)
                                                                                                                                    gATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCt            <  2:247988/148‑1 (MQ=255)
                                                                                                                                     aTGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTg           >  1:1554352/1‑148 (MQ=255)
                                                                                                                                      tGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTgg          >  1:2943889/1‑148 (MQ=255)
                                                                                                                                      tGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTgg          >  2:617592/1‑148 (MQ=255)
                                                                                                                                      tGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTgg          <  2:1130529/148‑1 (MQ=255)
                                                                                                                                         aTTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt       <  2:3272031/148‑1 (MQ=255)
                                                                                                                                         aTTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt       >  2:3533288/1‑148 (MQ=255)
                                                                                                                                          tttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATc      <  1:3416408/148‑1 (MQ=255)
                                                                                                                                           ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt     <  1:1024457/148‑1 (MQ=255)
                                                                                                                                           ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt     <  2:1039567/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTC  >  CP009273/142315‑142604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑