Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,574,523 T→A 100% L175F (TTA→TTT)  ydeM ← putative YdeN‑specific sulfatase‑maturating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,574,5230TA96.3% 715.0 / 11.9 223L175F (TTA→TTT) ydeMputative YdeN‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base T (8/0);  new base A (99/115);  total (108/115)
Fisher's exact test for biased strand distribution p-value = 2.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

TTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTG  >  CP009273/1574378‑1574667
                                                                                                                                                 |                                                                                                                                                
ttGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTaaa                                                                                                                                                <  1:3139091/148‑1 (MQ=255)
   aCATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTaaaaaa                                                                                                                                             >  1:423147/1‑148 (MQ=255)
   aCATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTaaaaaa                                                                                                                                             <  2:2298491/148‑1 (MQ=255)
     aTAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAAt                                                                                                                                           >  1:1147748/1‑148 (MQ=255)
      tAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATg                                                                                                                                          >  1:3285288/1‑148 (MQ=255)
      tAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATg                                                                                                                                          >  2:1918933/1‑148 (MQ=255)
      tAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATg                                                                                                                                          >  1:1578703/1‑148 (MQ=255)
       aaaCTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGa                                                                                                                                         <  2:1543379/148‑1 (MQ=255)
        aaCTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGAt                                                                                                                                        <  2:261138/148‑1 (MQ=255)
          cTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATaa                                                                                                                                      <  2:317485/148‑1 (MQ=255)
           ttGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATaaa                                                                                                                                     <  2:720704/148‑1 (MQ=255)
            tGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAAc                                                                                                                                    >  2:1083064/1‑148 (MQ=255)
             gCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAAcc                                                                                                                                   <  1:42950/148‑1 (MQ=255)
                 tAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACAGCCGATAGATTTAAAAAAATGATAAACCTCaa                                                                                                                               >  2:1452874/1‑148 (MQ=255)
                  aaGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAag                                                                                                                              >  1:2744535/1‑148 (MQ=255)
                   aGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAaga                                                                                                                             <  1:3167608/148‑1 (MQ=255)
                   aGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAaga                                                                                                                             <  1:80996/148‑1 (MQ=255)
                     ccGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAgg                                                                                                                           <  2:442236/148‑1 (MQ=255)
                     ccGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAgg                                                                                                                           <  2:3466765/148‑1 (MQ=255)
                     ccGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAgg                                                                                                                           <  2:193946/148‑1 (MQ=255)
                     ccGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAgg                                                                                                                           >  1:229653/1‑148 (MQ=255)
                     ccGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAgg                                                                                                                           >  1:2025833/1‑148 (MQ=255)
                       gTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGt                                                                                                                         >  2:3222585/1‑148 (MQ=255)
                       gTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGt                                                                                                                         <  1:1437960/148‑1 (MQ=255)
                           gAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATg                                                                                                                     >  1:1682686/1‑148 (MQ=255)
                              gCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGAc                                                                                                                  >  2:225342/1‑148 (MQ=255)
                               cacaGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACa                                                                                                                 <  2:3130242/148‑1 (MQ=255)
                                  aGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTa                                                                                                              <  1:2693318/148‑1 (MQ=255)
                                  aGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTa                                                                                                              >  2:269815/1‑148 (MQ=255)
                                  aGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTa                                                                                                              <  2:291446/148‑1 (MQ=255)
                                   gAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTaa                                                                                                             <  2:3433392/148‑1 (MQ=255)
                                       aaTCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGtt                                                                                                         <  1:1778333/148‑1 (MQ=255)
                                       aaTCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGtt                                                                                                         <  2:2145656/148‑1 (MQ=255)
                                        aTCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGtta                                                                                                        <  2:211017/148‑1 (MQ=255)
                                          cAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGttatt                                                                                                      >  2:851406/1‑148 (MQ=255)
                                           aaTGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGttatta                                                                                                     <  2:757846/148‑1 (MQ=255)
                                           aaTGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGttatta                                                                                                     <  1:1495444/148‑1 (MQ=255)
                                           aaTGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGttatta                                                                                                     <  2:1588730/148‑1 (MQ=255)
                                            aTGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTaa                                                                                                    <  2:240692/148‑1 (MQ=255)
                                              gATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATg                                                                                                  >  2:2639583/1‑148 (MQ=255)
                                                tACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGAc                                                                                                >  1:823893/1‑148 (MQ=255)
                                                 aCGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACg                                                                                               >  1:2664491/1‑148 (MQ=255)
                                                  cGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACgg                                                                                              <  1:460001/148‑1 (MQ=255)
                                                  cGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACgg                                                                                              <  1:1577115/148‑1 (MQ=255)
                                                  cGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACgg                                                                                              >  2:3065409/1‑148 (MQ=255)
                                                    gAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGtt                                                                                            >  2:251543/1‑148 (MQ=255)
                                                        gtgtTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACg                                                                                        >  2:1602171/1‑148 (MQ=255)
                                                          gtTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTa                                                                                      >  1:1014944/1‑148 (MQ=255)
                                                           ttCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTAt                                                                                     >  1:11294/1‑148 (MQ=255)
                                                              tcACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTaa                                                                                  >  1:3199442/1‑148 (MQ=255)
                                                                  tATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGt                                                                                       >  1:1646299/1‑139 (MQ=255)
                                                                     gACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTAc                                                                           >  1:2911841/1‑148 (MQ=255)
                                                                       ccACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACtt                                                                         <  2:553895/148‑1 (MQ=255)
                                                                           tGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGata                                                                     >  2:2595722/1‑148 (MQ=255)
                                                                            gAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGatat                                                                    >  2:3270487/1‑148 (MQ=255)
                                                                               aTCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGAt                                                                 <  1:2591548/148‑1 (MQ=255)
                                                                                tCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGAtt                                                                >  1:3530596/1‑148 (MQ=255)
                                                                                tCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGAtt                                                                >  1:1399828/1‑148 (MQ=255)
                                                                                tCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGAtt                                                                <  1:3088604/148‑1 (MQ=255)
                                                                                 cAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGAttt                                                               >  1:2064787/1‑148 (MQ=255)
                                                                                   atatTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTGACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCa                                                             <  1:2625826/148‑1 (MQ=255)
                                                                                    tatTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAg                                                            <  1:524897/148‑1 (MQ=255)
                                                                                    tatTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAg                                                            >  2:719466/1‑148 (MQ=255)
                                                                                    tatTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAAACTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTAAg                                                            >  2:528299/1‑148 (MQ=255)
                                                                                       tCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACg                                                         >  2:3084170/1‑148 (MQ=255)
                                                                                       tCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACg                                                         >  2:1313345/1‑148 (MQ=255)
                                                                                        cGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGc                                                        <  1:2544046/148‑1 (MQ=255)
                                                                                         ggCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCt                                                       >  1:1089781/1‑148 (MQ=255)
                                                                                          gCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTc                                                      >  2:493587/1‑148 (MQ=255)
                                                                                          gCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTc                                                      >  1:1863447/1‑148 (MQ=255)
                                                                                           cGTCCCGGTTTCTACCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCACGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCg                                                     <  2:83756/148‑1 (MQ=255)
                                                                                             tCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGAt                                                   <  1:3421364/148‑1 (MQ=255)
                                                                                               ccGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATgg                                                 <  2:3310669/148‑1 (MQ=255)
                                                                                                cGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGgc                                                >  2:1522703/1‑148 (MQ=255)
                                                                                                  gTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGgctg                                              <  2:489082/148‑1 (MQ=255)
                                                                                                   tttCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGgctgc                                             >  2:2614559/1‑148 (MQ=255)
                                                                                                       tAGCAATTCGATACATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATc                                         <  1:2502069/148‑1 (MQ=255)
                                                                                                        aGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCa                                        <  1:975985/148‑1 (MQ=255)
                                                                                                          cAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACt                                      >  2:1911914/1‑148 (MQ=255)
                                                                                                            aTTCGATAAATTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACttt                                    >  2:2947707/1‑148 (MQ=255)
                                                                                                                   aaaTTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCaaaa                             >  1:2025535/1‑148 (MQ=255)
                                                                                                                     aTTGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGt                           <  1:1918933/148‑1 (MQ=255)
                                                                                                                       tGCATATGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTAc                         >  1:917269/1‑148 (MQ=255)
                                                                                                                            atGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTa                    <  1:719446/148‑1 (MQ=255)
                                                                                                                             tGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTAc                   >  2:1734097/1‑148 (MQ=255)
                                                                                                                             tGTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTAc                   >  2:2521694/1‑148 (MQ=255)
                                                                                                                              gTTTACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTAcc                  <  2:456273/148‑1 (MQ=255)
                                                                                                                               tttACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCt                 <  2:1089781/148‑1 (MQ=255)
                                                                                                                                ttACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTCTTGCAAAAGTACCGTTACCTg                >  2:1162125/1‑148 (MQ=255)
                                                                                                                                 tACTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGa               <  2:1370698/148‑1 (MQ=255)
                                                                                                                                  aCTGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGaa              <  1:2433642/148‑1 (MQ=255)
                                                                                                                                    tGCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAAtt            >  2:3264998/1‑148 (MQ=255)
                                                                                                                                     gCCGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTa           <  2:823893/148‑1 (MQ=255)
                                                                                                                                      ccGATAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTAc          <  2:1320793/148‑1 (MQ=255)
                                                                                                                                        gATAGATTTAAAAAAATGATACACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTg        <  2:283384/148‑1 (MQ=255)
                                                                                                                                          tAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCg      <  2:229653/148‑1 (MQ=255)
                                                                                                                                          tAGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCg      <  2:2025969/148‑1 (MQ=255)
                                                                                                                                           aGATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTGACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGt     <  2:399859/148‑1 (MQ=255)
                                                                                                                                            gATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTc    >  2:1463241/1‑148 (MQ=255)
                                                                                                                                            gATTTAAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTc    >  1:3008087/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGACATAAACTTGCCATAAGCCGTGGGAGGCACAGAAAAATCAATGATACGGAATGTGTTCTCACTATGACCACTGAAATCAATATTCGGCGTCCCGGTTTCTAGCAATTCGATAAATTGCATATGTTTACTGCCGATAGATTTTAAAAAATGATAAACCTCAAGAGGGTAATGGACATTAACGTTATTAATGACGGTTAACGTATTAAACTCTACTTGATATGATTTCAGACGCTCGATGGCTGCTATCACTTTTGCAAAAGTACCGTTACCTGAATTACTGCGTCTG  >  CP009273/1574378‑1574667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑