Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,576,325 T→A 100% N153Y (AAT→TAT)  ydeN ← putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,576,3250TA97.9% 612.6 / 2.2 188N153Y (AAT→TAT) ydeNputative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base T (1/3);  new base A (108/76);  total (109/79)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

GCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGC  >  CP009273/1576181‑1576469
                                                                                                                                                |                                                                                                                                                
gcgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGa                                                                                                                                               <  1:2561988/148‑1 (MQ=255)
 cgTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGaa                                                                                                                                              >  2:3256418/1‑148 (MQ=255)
   tttgtttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATa                                                                                                                                            >  1:248155/1‑148 (MQ=255)
     tgtttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGa                                                                                                                                          >  2:145847/1‑148 (MQ=255)
        ttATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGacac                                                                                                                                       >  1:624434/1‑148 (MQ=255)
            cTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCaaa                                                                                                                                   >  1:1238275/1‑148 (MQ=255)
            cTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCaaa                                                                                                                                   >  2:876718/1‑148 (MQ=255)
            cTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATAGGTATAGGAATAGACACCaaa                                                                                                                                   <  2:1640625/148‑1 (MQ=255)
             ttCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAg                                                                                                                                  >  1:1233005/1‑148 (MQ=255)
               ccGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgcg                                                                                                                                <  1:2013066/148‑1 (MQ=255)
                   tACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAgcgggcg                                                                                                                            <  2:576962/148‑1 (MQ=255)
                   tACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgcgggcg                                                                                                                            >  2:2870394/1‑148 (MQ=255)
                   tACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgcgggcg                                                                                                                            <  1:489729/148‑1 (MQ=255)
                    aCCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAgcgggcgg                                                                                                                           <  2:495381/148‑1 (MQ=255)
                        gCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGc                                                                                                                       <  2:2250764/148‑1 (MQ=255)
                        gCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGc                                                                                                                       <  2:2105639/148‑1 (MQ=255)
                         cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCt                                                                                                                      <  1:49756/148‑1 (MQ=255)
                         cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCt                                                                                                                      >  1:2703741/1‑148 (MQ=255)
                         cacaTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCt                                                                                                                      >  1:2318178/1‑148 (MQ=255)
                           caTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                                                    <  1:589578/148‑1 (MQ=255)
                           caTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                                                    <  1:590760/148‑1 (MQ=255)
                           caTTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCg                                                                                                                    <  1:2459753/148‑1 (MQ=255)
                              tACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGAcc                                                                                                                 >  2:1408436/1‑148 (MQ=255)
                              tACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGAcc                                                                                                                 >  1:1044445/1‑148 (MQ=255)
                                cTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTCTAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCgg                                                                                                               >  2:1973883/1‑148 (MQ=255)
                                cTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCgg                                                                                                               >  1:3013120/1‑148 (MQ=255)
                                cTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATCCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCgg                                                                                                               >  1:3249892/1‑148 (MQ=255)
                                  gATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTc                                                                                                             >  2:1275622/1‑148 (MQ=255)
                                    tttttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCat                                                                                                           <  2:2155361/148‑1 (MQ=255)
                                     ttttGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCatt                                                                                                          >  1:849562/1‑148 (MQ=255)
                                          aCAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgc                                                                                                     >  1:3301051/1‑148 (MQ=255)
                                           cAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcg                                                                                                    >  2:1461703/1‑148 (MQ=255)
                                           cAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTgcg                                                                                                    <  2:440550/148‑1 (MQ=255)
                                                 ccATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCgg                                                                                              >  2:3468235/1‑148 (MQ=255)
                                                  cATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCggg                                                                                             >  2:2166511/1‑148 (MQ=255)
                                                   aTTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGa                                                                                            <  2:2468175/148‑1 (MQ=255)
                                                        ccTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGc                                                                                       >  1:41782/1‑148 (MQ=255)
                                                         cTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGcc                                                                                      >  2:3039097/1‑148 (MQ=255)
                                                         cTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGcc                                                                                      >  1:1039049/1‑148 (MQ=255)
                                                         cTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGcc                                                                                      >  2:3039146/1‑148 (MQ=255)
                                                         cTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGcc                                                                                      >  2:2062953/1‑148 (MQ=255)
                                                           aCTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCgg                                                                                    >  2:3049654/1‑148 (MQ=255)
                                                            ctgctgCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGa                                                                                   >  1:1792200/1‑148 (MQ=255)
                                                                tgcAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAaca                                                                               <  1:207162/148‑1 (MQ=255)
                                                                   aGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCg                                                                            >  2:249092/1‑148 (MQ=255)
                                                                     tgtAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCgtg                                                                          <  2:2225862/148‑1 (MQ=255)
                                                                     tgtAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCgtg                                                                          <  1:876718/148‑1 (MQ=255)
                                                                     tgtAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCgtg                                                                          <  1:1182701/148‑1 (MQ=255)
                                                                      gtAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCgtgt                                                                         >  1:3504630/1‑148 (MQ=255)
                                                                      gtAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCgtgt                                                                         >  2:2367628/1‑148 (MQ=255)
                                                                        aataaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGc                                                                       <  1:2393963/148‑1 (MQ=255)
                                                                          taaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCca                                                                     >  2:377584/1‑148 (MQ=255)
                                                                           aaCCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCcac                                                                    <  2:1908073/148‑1 (MQ=255)
                                                                             ccATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACAt                                                                  >  1:1840855/1‑148 (MQ=255)
                                                                             ccATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACAt                                                                  <  1:1546682/148‑1 (MQ=255)
                                                                              cATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATa                                                                 >  1:966437/1‑148 (MQ=255)
                                                                                     cTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTa                                                          <  2:1621270/148‑1 (MQ=255)
                                                                                     cTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTa                                                          <  1:838368/148‑1 (MQ=255)
                                                                                     cTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTa                                                          <  1:3420596/148‑1 (MQ=255)
                                                                                       ggAATAATTCAGGCAAGTAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGt                                                        <  2:2974144/148‑1 (MQ=255)
                                                                                       ggAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGt                                                        <  1:1062751/148‑1 (MQ=255)
                                                                                       ggAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGt                                                        >  2:283926/1‑148 (MQ=255)
                                                                                        gAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTa                                                       >  2:52862/1‑148 (MQ=255)
                                                                                        gAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTa                                                       >  1:1848080/1‑148 (MQ=255)
                                                                                          ataatTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTaaa                                                     >  1:2953853/1‑148 (MQ=255)
                                                                                          ataatTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTaaa                                                     <  1:60253/148‑1 (MQ=255)
                                                                                           taatTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTaaaa                                                    <  1:1443698/148‑1 (MQ=255)
                                                                                            aatTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAAc                                                   >  1:432251/1‑148 (MQ=255)
                                                                                             atTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACg                                                  >  2:1502439/1‑148 (MQ=255)
                                                                                              ttCAGGGAAGAAAGTTTCTGTTAGCTGAATACCATCCTGAGCATCGGTATAGGAATAGACACCACAGCGGGCGGGAGCTCGACCGGTCATTATTGTGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATATCCGTTAGTAAAACGt                                                 <  1:1738928/148‑1 (MQ=255)
                                                                                              ttCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGTGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGt                                                 <  1:1154592/148‑1 (MQ=255)
                                                                                                  ggCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGc                                             <  1:539131/148‑1 (MQ=255)
                                                                                                   gCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGcc                                            >  1:35665/1‑148 (MQ=255)
                                                                                                   gCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGcc                                            <  1:1942609/148‑1 (MQ=255)
                                                                                                   gCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAAACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGcc                                            >  1:976845/1‑148 (MQ=255)
                                                                                                    cAAGAAAGTTTCTGTTAGCGGAATACCACCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCt                                           >  1:343508/1‑148 (MQ=255)
                                                                                                      aGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTc                                         >  1:2738652/1‑148 (MQ=255)
                                                                                                       gAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCa                                        <  2:279171/148‑1 (MQ=255)
                                                                                                        aaaGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCAt                                       <  2:1044445/148‑1 (MQ=255)
                                                                                                        aaaGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACAACGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCAt                                       >  2:912403/1‑148 (MQ=255)
                                                                                                         aaGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATc                                      >  2:1743557/1‑148 (MQ=255)
                                                                                                            tttCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAATGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAt                                   >  2:760875/1‑148 (MQ=255)
                                                                                                             ttCTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCAtt                                  >  1:3238261/1‑148 (MQ=255)
                                                                                                               cTGTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTaa                                <  2:3356345/148‑1 (MQ=255)
                                                                                                                 gTTAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATg                              <  1:2240103/148‑1 (MQ=255)
                                                                                                                   tAGCGGAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGaa                            >  1:2162324/1‑148 (MQ=255)
                                                                                                                       ggAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGa                        >  1:3465476/1‑148 (MQ=255)
                                                                                                                       ggAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGa                        <  2:604983/148‑1 (MQ=255)
                                                                                                                        gAATACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAg                       <  2:1129106/148‑1 (MQ=255)
                                                                                                                         aaTACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGc                      >  2:107045/1‑148 (MQ=255)
                                                                                                                         aaTACCATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGc                      >  2:667548/1‑148 (MQ=255)
                                                                                                                            aCCGTCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTc                   >  1:689858/1‑148 (MQ=255)
                                                                                                                             ccATCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCg                  >  1:2106619/1‑148 (MQ=255)
                                                                                                                              cATCCTGAGCATCGGAATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTACATACATTAATGAAAGGAGCGTCgg                 >  2:2661142/1‑148 (MQ=255)
                                                                                                                                tCCTGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGTAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCg               >  1:1757775/1‑148 (MQ=255)
                                                                                                                                   tGAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTg            >  2:2845312/1‑148 (MQ=255)
                                                                                                                                    gAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGa           <  1:3039146/148‑1 (MQ=255)
                                                                                                                                    gAGCATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGa           <  1:3039129/148‑1 (MQ=255)
                                                                                                                                       cATCGGTATAGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGAttt        <  2:1647316/148‑1 (MQ=255)
                                                                                                                                             tataGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGc  >  1:1948530/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCGTTTGTTTATCTTCCGGTACCGGCACATTACTGATTTTTGACAAGTGCCATTTACCTACTGCTGCAGTGTAATAACCATGATTCTGGAATAATTCAGGCAAGAAAGTTTCTGTTAGCGGAATACCATCCTGAGCATCGGTATTGGAATAGACACCAAAGCGGGCGGGAGCTCGACCGGTCATTATTGCGGCGCGGGAGGGGCCGGAAACACCGTGTGCCACATAGCCGTTAGTAAAACGTACGCCTTCATCCATTAATGAAAGGAGCGTCGGCGTTGATTTTTGTGC  >  CP009273/1576181‑1576469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑