Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,603,440 G→A 100% intergenic (‑161/+46) yneE ← / ← uxaB bestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,603,4400GA100.0% 591.5 / NA 181intergenic (‑161/+46)yneE/uxaBbestrophin family putative inner membrane protein/altronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (98/83);  total (98/83)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 184 total aligned reads displayed.

TGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTC  >  CP009273/1603295‑1603584
                                                                                                                                                 |                                                                                                                                                
   aaaaGTCGCCATATTAAACGTGATTACTATCACATTCTCAAGACGCTAATTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGAACCTGTTAATGATAAAGGCATATAGGATGTTGTAACAAATATGGTAAAATa                                                                                                                                             <  2:2422927/148‑1 (MQ=255)
      aGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGa                                                                                                                                          >  2:1829242/1‑148 (MQ=255)
      aGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGa                                                                                                                                          >  2:728869/1‑148 (MQ=255)
       gTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGaa                                                                                                                                         <  2:1012989/148‑1 (MQ=255)
        tCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGaaa                                                                                                                                        <  2:3474638/148‑1 (MQ=255)
        tCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGaaa                                                                                                                                        <  1:163862/148‑1 (MQ=255)
          gCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAAcc                                                                                                                                      <  1:332571/148‑1 (MQ=255)
              tATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGt                                                                                                                                  >  1:2665019/1‑148 (MQ=255)
              tATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGt                                                                                                                                  >  2:2015691/1‑148 (MQ=255)
               aTTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTc                                                                                                                                 >  1:571796/1‑148 (MQ=255)
               aTTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTc                                                                                                                                 >  1:3021337/1‑148 (MQ=255)
               aTTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTc                                                                                                                                 >  2:2800530/1‑148 (MQ=255)
                 tAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGa                                                                                                                               <  2:343568/148‑1 (MQ=255)
                  aaaCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGaa                                                                                                                              <  2:1895165/148‑1 (MQ=255)
                  aaaCGTGATTACGATCACATTCTCAAGACGCTAATTACAAATTAGCTAAAAGCTTACAACATTAGCAGGCGCTGAATGTGGAACCTGTTTTTGATAAAGGAATATAGGTTTTTGTATCTAATATGGTAATATTAGTAAACGGGTCGat                                                                                                                              <  2:1699112/148‑2 (MQ=255)
                   aaCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGaaa                                                                                                                             >  1:438649/1‑148 (MQ=255)
                   aaCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGaaa                                                                                                                             <  2:1880965/148‑1 (MQ=255)
                    aCGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAAc                                                                                                                            <  2:2537134/148‑1 (MQ=255)
                     cGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAAcc                                                                                                                           <  1:433506/148‑1 (MQ=255)
                         aTTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGt                                                                                                                       >  2:3249817/1‑148 (MQ=255)
                          ttACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGtt                                                                                                                      >  1:590900/1‑148 (MQ=255)
                           tACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTc                                                                                                                     <  2:679221/148‑1 (MQ=255)
                            aCGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCa                                                                                                                    >  1:2270017/1‑148 (MQ=255)
                            aCGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCa                                                                                                                    >  2:2591860/1‑148 (MQ=255)
                               aTCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGaa                                                                                                                 >  1:2811050/1‑148 (MQ=255)
                               aTCACATTCTCAAGACGCTACTTACAAATGACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGaa                                                                                                                 <  2:404671/148‑1 (MQ=255)
                                 cacaTTCTCAAGACGCTGCTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGt                                                                                                               >  2:1854797/1‑148 (MQ=255)
                                 cacaTTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGCTCAGAAGt                                                                                                               <  1:2694630/148‑1 (MQ=255)
                                  acaTTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTa                                                                                                              >  2:1642459/1‑148 (MQ=255)
                                     ttCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCTGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACg                                                                                                           <  2:3557128/148‑1 (MQ=255)
                                     ttCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAgcaccg                                                                                                           >  2:1305807/1‑143 (MQ=255)
                                     ttCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACg                                                                                                           >  1:2522973/1‑148 (MQ=255)
                                     ttCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACg                                                                                                           >  2:2821388/1‑148 (MQ=255)
                                       ctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATTGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgt                                                                                                         <  1:1178966/148‑1 (MQ=255)
                                       ctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAACTAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgt                                                                                                         <  2:3340066/148‑1 (MQ=255)
                                       ctcAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGgt                                                                                                         <  2:3481956/148‑1 (MQ=255)
                                          aaGACGCTACTTACAAATTACCTACAAGCTTACAACATTACCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGtt                                                                                                      <  2:2734275/148‑1 (MQ=255)
                                           aGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGtta                                                                                                     >  1:2262150/1‑148 (MQ=255)
                                            gACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGttat                                                                                                    <  2:1475149/148‑1 (MQ=255)
                                            gACGCTACTTACAAATTACCTACAAGCTTACAACATGAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGttat                                                                                                    <  2:3335036/148‑1 (MQ=255)
                                                    ttACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGACGTAACGGTGTTATTAGCACaa                                                                                            >  1:90819/1‑148 (MQ=255)
                                                         aaTTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGAc                                                                                       <  1:3296058/148‑1 (MQ=255)
                                                          aTTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACg                                                                                      <  2:3188022/148‑1 (MQ=255)
                                                            tACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTNACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTa                                                                                    >  1:1806996/1‑148 (MQ=255)
                                                            tACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTa                                                                                    <  2:2328942/148‑1 (MQ=255)
                                                             aCCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTAc                                                                                   <  2:55354/148‑1 (MQ=255)
                                                               cTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAg                                                                                 >  1:1293522/1‑148 (MQ=255)
                                                               cTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAg                                                                                 >  1:1293297/1‑148 (MQ=255)
                                                                 aCAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCt                                                                               >  2:2951716/1‑148 (MQ=255)
                                                                   aaGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTc                                                                             >  1:1999242/1‑148 (MQ=255)
                                                                         acaacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAt                                                                       <  1:447661/148‑1 (MQ=255)
                                                                         acaacaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCAt                                                                       <  2:3021514/148‑1 (MQ=255)
                                                                          caaccTGAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATc                                                                      <  1:431172/148‑1 (MQ=255)
                                                                            acaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATccc                                                                    <  1:1039901/148‑1 (MQ=255)
                                                                            acaTTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATccc                                                                    >  1:3393972/1‑148 (MQ=255)
                                                                               ttAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCttt                                                                 <  1:817102/148‑1 (MQ=255)
                                                                                tAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCtttt                                                                >  1:1404965/1‑148 (MQ=255)
                                                                                tAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCtttt                                                                >  1:1406089/1‑148 (MQ=255)
                                                                                tAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCtttt                                                                >  2:791340/1‑148 (MQ=255)
                                                                                tAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCtttt                                                                <  1:3115436/148‑1 (MQ=255)
                                                                                   cAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCg                                                             <  2:853810/148‑1 (MQ=255)
                                                                                    aGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCga                                                            >  1:1748412/1‑148 (MQ=255)
                                                                                    aGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCga                                                            <  1:3181794/148‑1 (MQ=255)
                                                                                      gcgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCgaga                                                          >  1:2828104/1‑148 (MQ=255)
                                                                                      gcgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCgaga                                                          >  1:2325665/1‑148 (MQ=255)
                                                                                      gcgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCgaga                                                          <  2:2522973/148‑1 (MQ=255)
                                                                                       cgcTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGaa                                                         <  1:3423929/148‑1 (MQ=255)
                                                                                              tgtgGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATc                                                  <  1:1673352/148‑1 (MQ=255)
                                                                                                 ggCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAg                                               >  2:574317/1‑148 (MQ=255)
                                                                                                 ggCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAg                                               <  1:2616934/148‑1 (MQ=255)
                                                                                                   cACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGt                                             <  1:1493552/148‑1 (MQ=255)
                                                                                                   cACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGt                                             >  2:2253735/1‑148 (MQ=255)
                                                                                                       tGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCAtt                                         >  1:3532007/1‑148 (MQ=255)
                                                                                                        gTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTa                                        >  1:3239134/1‑148 (MQ=255)
                                                                                                          tAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGc                                      >  1:3122632/1‑148 (MQ=255)
                                                                                                          tAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGc                                      >  1:1164844/1‑148 (MQ=255)
                                                                                                              gATAAAGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAAcc                                  >  2:647443/1‑148 (MQ=255)
                                                                                                                  aaGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCt                              <  1:791407/148‑1 (MQ=255)
                                                                                                                   aGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTc                             >  1:2055665/1‑148 (MQ=255)
                                                                                                                   aGGCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTc                             >  2:3427399/1‑148 (MQ=255)
                                                                                                                    ggCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCg                            >  2:858854/1‑148 (MQ=255)
                                                                                                                     gCATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCga                           >  1:1049938/1‑148 (MQ=255)
                                                                                                                      cATATAGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgac                          <  1:194802/148‑1 (MQ=255)
                                                                                                                         ataGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccag                       >  2:2663662/1‑148 (MQ=255)
                                                                                                                          taGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccaga                      <  1:1384228/148‑1 (MQ=255)
                                                                                                                           aGGATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagac                     >  2:3012797/1‑148 (MQ=255)
                                                                                                                             gATGTTGTAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCgaccagacca                   >  2:1982582/1‑148 (MQ=255)
                                                                                                                               tgttgtAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAgg                 <  1:728869/148‑1 (MQ=255)
                                                                                                                               tgttgtAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAgg                 <  2:664443/148‑1 (MQ=255)
                                                                                                                               tgttgtAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAgg                 <  2:1613256/148‑1 (MQ=255)
                                                                                                                                gttgtAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGc                >  1:853843/1‑148 (MQ=255)
                                                                                                                                gttgtAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGc                >  1:614986/1‑148 (MQ=255)
                                                                                                                                gttgtAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGc                >  1:1532130/1‑148 (MQ=255)
                                                                                                                                   gtAACTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACt             <  1:2097347/148‑1 (MQ=255)
                                                                                                                                     aaCTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTg           >  1:466516/1‑148 (MQ=255)
                                                                                                                                     aaCTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTg           >  1:1471864/1‑148 (MQ=255)
                                                                                                                                      aCTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTA‑TAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCg         >  2:1243499/1‑148 (MQ=255)
                                                                                                                                       cTAATATGGTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCCTTAGCAACCTGCTCGACCAGAGCAGGCACTTGCg         >  2:3177732/1‑148 (MQ=255)
                                                                                                                                           tatGGTAAAATAAGAAACCAGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAg     <  1:2778756/148‑1 (MQ=255)
                                                                                                                                              ggTAAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTc  <  2:1509343/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAAGGCATATAGGATGTTGTAACTAATATGGTGAAATAAGAAACCCGGTCGAAACCGGGTTCAGAAGTAACGGTGTTATTAGCACAACGGACGTACAGCTTCGCGCATCCCTTTTTCGAGAATCGCATCCAGGTCATTAGCAACCTGCTCGACCAGACCAGGCACTTGCGTCAGGTC  >  CP009273/1603295‑1603584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑