Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 159,666 G→T 100% G359C (GGT→TGT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273159,6660GT98.7% 527.0 / ‑1.7 171G359C (GGT→TGT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (86/82);  total (88/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 171 total aligned reads displayed.

ACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCG  >  CP009273/159521‑159809
                                                                                                                                                 |                                                                                                                                               
      tAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACACGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTg                                                                                                                                         <  2:599288/148‑1 (MQ=255)
      tAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTg                                                                                                                                         <  2:1570150/148‑1 (MQ=255)
      tAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTg                                                                                                                                         <  1:1241948/148‑1 (MQ=255)
       aGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGa                                                                                                                                        <  1:906504/148‑1 (MQ=255)
       aGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTGTGCTGa                                                                                                                                        <  2:2547251/148‑1 (MQ=255)
             gCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAAt                                                                                                                                  >  2:2439641/1‑148 (MQ=255)
               atccatccCGCAGCCTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCAGTTAATCGCCAAAGAACCCGCAGAACGCGCCGCGGCGCCAAGTGAACCGGAGAACTTACACAGCGATCTTTCCTGATTGCTGATGGAAtga                                                                                                                                <  2:2347236/148‑3 (MQ=255)
               aTCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTa                                                                                                                                <  2:560182/148‑1 (MQ=255)
                tCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACCAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTAc                                                                                                                               >  2:3480368/1‑148 (MQ=255)
                tCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTAc                                                                                                                               <  1:3070425/148‑1 (MQ=255)
                    tGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCa                                                                                                                           >  1:1459116/1‑148 (MQ=255)
                    tGACGCAGCGTGCCGGGCGCGCCGGGCGTCAGGAGCCGGCTATCAGCCTGCATTTAAACGCCAAAGAACAAGCAGAACGCGCCGCGGCGCCAACTGAACCGGCGATATTACAAAGCGAACTTACCTGATTGATGATGGAATTAcatcc                                                                                                                           >  1:1459198/1‑144 (MQ=255)
                      aCGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAAt                                                                                                                         >  2:428138/1‑148 (MQ=255)
                            cGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGAt                                                                                                                   >  1:1687871/1‑148 (MQ=255)
                            cGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGAt                                                                                                                   <  2:1491830/148‑1 (MQ=255)
                             gTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGACAAAGAACAAGCAGAACGCGCCTCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATg                                                                                                                  <  2:3038843/148‑1 (MQ=255)
                              tGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGc                                                                                                                 <  1:1135782/148‑1 (MQ=255)
                               gCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCa                                                                                                                <  1:3218038/148‑1 (MQ=255)
                                ccGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAg                                                                                                               <  2:1072911/148‑1 (MQ=255)
                                   ggCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTAAAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGa                                                                                                            >  1:13113/1‑148 (MQ=255)
                                           gggCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGAAAAGTGAAGCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGgcgc                                                                                                    <  1:1546601/148‑1 (MQ=255)
                                             gCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAg                                                                                                  >  1:478905/1‑148 (MQ=255)
                                             gCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAg                                                                                                  >  2:2520526/1‑148 (MQ=255)
                                             gCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAg                                                                                                  <  2:1924585/148‑1 (MQ=255)
                                              cGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGa                                                                                                 <  2:1161820/148‑1 (MQ=255)
                                               gTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGAt                                                                                                <  1:2628629/148‑1 (MQ=255)
                                               gTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGAt                                                                                                <  1:851022/148‑1 (MQ=255)
                                                tCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATg                                                                                               <  1:1114809/148‑1 (MQ=255)
                                                 cTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGa                                                                                              >  1:2738836/1‑148 (MQ=255)
                                                   ggAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGCGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTCCTGCAATGGGGATGCAGCGATCCGGCGCAGATGAgc                                                                                            <  1:3148173/148‑1 (MQ=255)
                                                      gCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAgctgg                                                                                         <  1:2906474/148‑1 (MQ=255)
                                                          ggTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGctggctgg                                                                                     >  2:2906164/1‑148 (MQ=255)
                                                           gTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGa                                                                                    >  1:2964543/1‑148 (MQ=255)
                                                            tATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGAt                                                                                   >  2:1988828/1‑148 (MQ=255)
                                                             aTCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATc                                                                                  >  2:2082801/1‑148 (MQ=255)
                                                               cAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCaa                                                                                <  2:1666025/148‑1 (MQ=255)
                                                                 gCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAAcc                                                                              >  2:3380078/1‑148 (MQ=255)
                                                                  ccTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCg                                                                             <  2:672650/148‑1 (MQ=255)
                                                                     gCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCa                                                                          >  2:2039216/1‑148 (MQ=255)
                                                                      cATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCag                                                                         <  1:291573/148‑1 (MQ=255)
                                                                        tttAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCagta                                                                       >  1:914433/1‑148 (MQ=255)
                                                                            aTCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCACTGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGa                                                                   >  1:1066579/1‑148 (MQ=255)
                                                                            aTCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGa                                                                   >  1:2120760/1‑148 (MQ=255)
                                                                            aTCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGa                                                                   >  2:1265041/1‑148 (MQ=255)
                                                                            aTCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGa                                                                   >  1:2842483/1‑148 (MQ=255)
                                                                             tCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGaa                                                                  >  2:2352117/1‑148 (MQ=255)
                                                                             tCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGaa                                                                  <  1:2581335/148‑1 (MQ=255)
                                                                               gCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATc                                                                <  1:1962674/148‑1 (MQ=255)
                                                                                ccAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCt                                                               <  1:2651737/148‑1 (MQ=255)
                                                                                 cAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTa                                                              >  2:233481/1‑148 (MQ=255)
                                                                                  aaaGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTAc                                                             >  2:1354124/1‑148 (MQ=255)
                                                                                   aaGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACt                                                            <  1:1151454/148‑1 (MQ=255)
                                                                                   aaGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACGGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACt                                                            <  1:630437/148‑1 (MQ=255)
                                                                                    aGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTg                                                           >  1:887479/1‑148 (MQ=255)
                                                                                        cAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCcg                                                       <  1:283615/148‑1 (MQ=255)
                                                                                          aGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCcgcg                                                     >  1:2339418/1‑148 (MQ=255)
                                                                                          aGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCcgcg                                                     >  2:1764897/1‑148 (MQ=255)
                                                                                          aGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCcgcg                                                     >  2:1764857/1‑148 (MQ=255)
                                                                                           gCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTTCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGa                                                    >  1:1839141/1‑148 (MQ=255)
                                                                                           gCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGa                                                    >  2:2486410/1‑148 (MQ=255)
                                                                                           gCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGa                                                    >  2:2485846/1‑148 (MQ=255)
                                                                                            cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGaa                                                   <  2:3216229/148‑1 (MQ=255)
                                                                                            cAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGaa                                                   <  2:1864895/148‑1 (MQ=255)
                                                                                               aaCGCGCCGCGGCGCACAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAACGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACg                                                <  1:2094438/148‑1 (MQ=255)
                                                                                               aaCGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACg                                                <  1:2322765/148‑1 (MQ=255)
                                                                                               aaCGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACg                                                >  1:393757/1‑148 (MQ=255)
                                                                                                aCGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGt                                               <  2:467178/148‑1 (MQ=255)
                                                                                                aCGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGt                                               >  1:963252/1‑148 (MQ=255)
                                                                                                 cgcgCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTc                                              <  1:161350/148‑1 (MQ=255)
                                                                                                 cgcgCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTc                                              >  1:179415/1‑148 (MQ=255)
                                                                                                   cgccgcGGCGCAAAGTGAACCGGAGATCTTACAACGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTg                                            <  2:2715239/148‑1 (MQ=255)
                                                                                                         ggcgCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACaa                                      <  2:3289651/148‑1 (MQ=255)
                                                                                                          gcgcAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACaaa                                     <  2:2323884/148‑1 (MQ=255)
                                                                                                            gcAAAGTGAACCGGAGATCTGACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATg                                   <  1:3318169/148‑1 (MQ=255)
                                                                                                             cAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGc                                  <  1:2082801/148‑1 (MQ=255)
                                                                                                               aaGTGAACCGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTg                                >  1:1406290/1‑148 (MQ=255)
                                                                                                               aaGTGAACCGGAGATCTTACAAAGCGATCATTCCTGTTTGCTGATGGAATTACTGCACTGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGACACGTCTGTTACAAATGCTg                                >  1:1405872/1‑148 (MQ=255)
                                                                                                                      ccGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCAc                         <  1:3179961/148‑1 (MQ=255)
                                                                                                                      ccGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCAc                         >  1:3263063/1‑148 (MQ=255)
                                                                                                                      ccGGAGATCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCAc                         >  1:3263227/1‑148 (MQ=255)
                                                                                                                           gaTCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCCCTGGAg                    >  2:2942707/1‑148 (MQ=255)
                                                                                                                           gaTCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAg                    <  2:36932/148‑1 (MQ=255)
                                                                                                                            aTCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAgg                   >  1:2809153/1‑148 (MQ=255)
                                                                                                                            aTCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAgg                   <  2:1585048/148‑1 (MQ=255)
                                                                                                                             tCTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAggg                  <  2:685178/148‑1 (MQ=255)
                                                                                                                              cTTACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGt                 <  1:2915576/148‑1 (MQ=255)
                                                                                                                                tACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGa               <  2:2942603/148‑1 (MQ=255)
                                                                                                                                tACAAAGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGa               <  2:2277073/148‑1 (MQ=255)
                                                                                                                                   aaaGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACg            <  1:509103/148‑1 (MQ=255)
                                                                                                                                   aaaGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACg            >  1:525967/1‑148 (MQ=255)
                                                                                                                                    aaGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACgg           >  1:1669864/1‑148 (MQ=255)
                                                                                                                                    aaGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACgg           >  1:1147857/1‑148 (MQ=255)
                                                                                                                                     aGCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGc          >  2:2201542/1‑148 (MQ=255)
                                                                                                                                      gCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCt         <  1:2038860/148‑1 (MQ=255)
                                                                                                                                      gCGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCt         <  1:740033/148‑1 (MQ=255)
                                                                                                                                      gCGATCTTTCCTGTTTGCTGATGGAATTACCGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCt         <  1:2039230/148‑1 (MQ=255)
                                                                                                                                       cGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCtt        >  2:2776920/1‑148 (MQ=255)
                                                                                                                                       cGATCTTTCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCtt        >  1:3378439/1‑148 (MQ=255)
                                                                                                                                       cGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCtt        >  1:3472251/1‑148 (MQ=255)
                                                                                                                                             ttCCTGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTgcg  >  1:1438195/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCGGGCGCGCCGGGCGTCTGGAGCCGGGTATCAGCCTGCATTTAATCGCCAAAGAACAAGCAGAACGCGCCGCGGCGCAAAGTGAACCGGAGATCTTACAAAGCGATCTTTCCGGTTTGCTGATGGAATTACTGCAATGGGGATGCAGCGATCCGGCGCAGATGAGCTGGCTGGATCAACCGCCAGTAGTGAATCTACTGGCCGCGAAACGTCTGTTACAAATGCTGGGGGCACTGGAGGGTGAACGGCTTAGTGCG  >  CP009273/159521‑159809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑